scholarly journals Hairy root culture of Lactuca virosa L.

2014 ◽  
Vol 64 (1) ◽  
pp. 33-39 ◽  
Author(s):  
Anna Stojakowska ◽  
Janusz Malarz ◽  
Wanda Kisiel ◽  
Stanisław Kohlmünzer
Keyword(s):  
Growth Rate ◽  
Hairy Root ◽  
Chromatographic Analysis ◽  
Sesquiterpene Lactones ◽  
Leaf Explants ◽  
Root Culture ◽  
Biomass Accumulation ◽  
Hairy Root Culture ◽  
Nutrient Media ◽  
Lactuca Virosa

A hairy root culture of <i>Lactuca virosa</i> L. (<i>Asteraceae</i>) was initiated by infection of the leaf explants of the aseptic plants with <i>Agrobacterium rhizogenes</i> - strain LBA 9402. One of the obtained clones which showed the most favourable growth rate was used for further investigation. An influence of different nutrient media and sucrose concentrations on the biomass accumulation was studied. The most advantageous for the biomass increment were media: MS and MS containing macronutrients reduced to half concentration, supplemented with 5% sucrose. A preliminary chromatographic analysis of the tissue extract revealed the presence of sesquiterpene lactones characteristic of the roots of the intact plant.

ChemInform Abstract: Sesquiterpene Lactones in Agrobacterium rhizogenes - Transformed Hairy Root Culture of Lactuca virosa

ChemInform ◽  
2010 ◽  
Vol 27 (9) ◽  
pp. no-no
Author(s):  
W. KISIEL ◽  
A. STOJAKOWSKA ◽  
J. MALARZ ◽  
S. KOHLMUENZER
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Sesquiterpene Lactones ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Lactuca Virosa ◽  
Transformed Hairy Root

scholarly journals Effect of pectinase on the production of sesquiterpene lactones in the hairy root culture of Lactuca virosa L.

2014 ◽  
Vol 69 (2) ◽  
pp. 115-117 ◽  
Author(s):  
Janusz Malarz ◽  
Wanda Kisiel
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Sesquiterpene Lactones ◽  
Control Level ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Lactuca Virosa ◽  
Direct Addition

Direct addition of pectinase as an elicitor to the hairy root culture of <em>Lactuca virosa</em> L. transformed with <em>Agrobacterium rhizogenes</em> stimulates the accumulation of constitutive sesquiterpene lactones - lactuside A and crepidiaside B. The highest amounts of lactuside A (80% above the control level) and crepidiaside B (39% above the control level) have been found 24 and 48 hours, respectively, after pectinase treatment.

Sesquiterpene lactones in Agrobacterium rhizogenes—Transformed hairy root culture of Lactuca virosa

1995 ◽  
Vol 40 (4) ◽  
pp. 1139-1140 ◽  
Author(s):  
Wanda Kisiel ◽  
Anna Stojakowska ◽  
Janusz Malarz ◽  
Stanislaw Kohlmüzer
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Sesquiterpene Lactones ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Lactuca Virosa ◽  
Transformed Hairy Root

scholarly journals Effect of methyl jasmonate on the production of sesquiterpene lactones in the hairy root culture of Lactuca virosa L.

2014 ◽  
Vol 68 (2) ◽  
pp. 119-121 ◽  
Author(s):  
Janusz Malarz ◽  
Wanda Kisiel
Keyword(s):  
Methyl Jasmonate ◽  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
Hplc Analysis ◽  
Sesquiterpene Lactones ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Lactuca Virosa ◽  
Methyl Jasmonate Treatment

Hairy roots of <em>Lactuca virosa</em> L. transformed with <em>Agrobacterium rhizogenes</em> and treated with methyl jasmonate, are capable of producing sesquiterpene lactones - crepidiaside B and lactuside A in amounts higher than in untreated roots. An HPLC analysis showed the highest content of crepidiaside B after 24 hours of methyl jasmonate treatment (60% above the control). The increase in the content of lactuside A was relatively small and reached 27% after 48 hours of the treatment, in comparison with the control.

Sesquiterpene Lactones in a Hairy Root Culture of Cichorium intybus

2002 ◽  
Vol 57 (11-12) ◽  
pp. 994-997 ◽  
Author(s):  
Janusz Malarz ◽  
Anna Stojakowska ◽  
Wanda Kisiel
Keyword(s):  
Stationary Phase ◽  
Sesquiterpene Lactone ◽  
Hairy Root ◽  
Sesquiterpene Lactones ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Cichorium Intybus ◽  
Early Stationary Phase ◽  
Transformed Root Culture

A transformed root culture of Cichorium intybus L. (Asteraceae) was found to produce sesquiterpene lactones of guaiane and germacrane type. Lactucopicrin, 8-desoxylactucin and three sesquiterpene lactone glycosides: crepidiaside B, sonchuside A and ixerisoside D were isolated from the roots. The yield of 8-desoxylactucin reached 0.03 g l-1 at the early stationary phase of the culture.

scholarly journals Optimization of Carbon Source for Hairy Root Growth and Withaferin A and Withanone Production in Withania somnifera

2012 ◽  
Vol 7 (10) ◽  
pp. 1934578X1200701 ◽  
Author(s):  
Ganeshan Sivanandhan ◽  
Manoharan Rajesh ◽  
Muthukrishnan Arun ◽  
Murugaraj Jeyaraj ◽  
Gnanajothi Kapil Dev ◽  
...  
Keyword(s):  
Carbon Source ◽  
Liquid Medium ◽  
Hairy Root ◽  
Root Biomass ◽  
Withania Somnifera ◽  
Carbon Sources ◽  
Root Culture ◽  
Biomass Accumulation ◽  
Hairy Root Culture ◽  
Withaferin A

This study optimized carbon sources in half MS liquid medium for maximum biomass accumulation and withanolides production in hairy root culture of Withania somnifera. The highest production of withaferin A and withanone was achieved when sucrose and sucrose+glucose were used individually as carbon sources. The hairy root suspension culture supplemented with a lower level of sucrose (2%) favored hairy root biomass accumulation (1.41 g DW) followed by sucrose+glucose (2+1) when compared with other carbon sources in half MS liquid medium after 40 days of culture. The hairy roots grown on sucrose (4%) enriched half MS liquid medium stimulated higher production of withaferin A (2.21 mg/g DW) and withanone (2.41 mg/g DW) on the 40th day of culture, followed by sucrose+glucose (4+1%) compared with glucose, fructose, maltose and other combinations tested.

scholarly journals Caffeic acid derivatives from a hairy root culture of Lactuca virosa

2011 ◽  
Vol 34 (1) ◽  
pp. 291-298 ◽  
Author(s):  
Anna Stojakowska ◽  
Janusz Malarz ◽  
Agnieszka Szewczyk ◽  
Wanda Kisiel
Keyword(s):  
Caffeic Acid ◽  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Caffeic Acid Derivatives ◽  
Lactuca Virosa

scholarly journals E-p-methoxycinnamic acid production in hairy root cultures of Scrophularia buergeriana Miquel

2010 ◽  
Vol 62 (3) ◽  
pp. 649-652 ◽  
Author(s):  
Sang Park ◽  
Xiaohua Li ◽  
Seok Eom ◽  
Chung Lee ◽  
Sook Lee
Keyword(s):  
Hairy Root ◽  
Leaf Explants ◽  
Root Culture ◽  
Dry Weight ◽  
Hairy Root Culture ◽  
In Vitro Production ◽  
Methoxycinnamic Acid ◽  
Half Strength ◽  
Vitro Production

E-p-methoxycinnamic acid (MCA) is one of the main active constituents of Scrophularia buergeriana Miquel and has hepatoprotective, anti-amnestic, and neuroprotective activities. For studying in vitro production of MCA, we established a hairy root culture of S. buergeriana by infecting leaf explants with Agrobacterium rhizogenes R1000, and tested the growth and MCA production of these cultures using different strengths of media and concentrations of auxins. Hairy roots grown in half-strength MS medium showed the highest levels of growth (10.3 g/l) as well as MCA production (0.83 mg/100 g dry weight). Hairy root culture with the treatment of 0.5 mg/l IBA produced the highest amount of dry weight (11.8 g/l) and MCA (1.26 mg/100 g dry weight) production. These results demonstrate that the hairy root culture of S. buergeriana is a valuable alternative approach for the production of MCA. .

scholarly journals Hairy Root Induction on Justicia gendarussa by Various Density of Agrobacterium rhizogenes strain LB 510

2017 ◽  
Vol 9 (1) ◽  
pp. 26
Author(s):  
Dwi Kusuma Wahyuni ◽  
Siti Nur Hafida ◽  
Tri Muji Ermayanti ◽  
Bambang Prajoga Eko Wardoyo ◽  
Hery Purnobasuki ◽  
...  
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Roots ◽  
Solid Medium ◽  
Leaf Explants ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Root Induction ◽  
Hairy Root Induction ◽  
Industrial Needs

<p>Gandarusa (<em>Justicia gendarussa</em> Burm.f.) is an Indonesian medicinal plant that has many benefits as drug and male contracetive. For industrial needs, Gandarusa must be  available in large quantity. Hairy root culture is one of methode to produce phytochemistry compound. The objective of the study was to examine the effect of various density of <em>Agrobacterium rhizogenes</em> strain LB510 on hairy roots induction of gandarusa (<em>Justicia gendarussa </em>Burm.f.) leaf plant. Leaf explants were inoculated in MS liquid medium with various density of OD<sub>600</sub> = 0.1; 0.2; 0.3; 0.4; and 0.5. Explants were co-cultivated for 2 days on MS solid medium without any hormone then sub-cultured on MS solid medium containing antibiotic cefotaxim 300 ppm, in dark condition. The data were analyzed descriptively and statistically. The results showed that various density of <em>Agrobacterium rhizogenes</em> strain LB510 was affected the lenght of hairy roots induction of <em>J</em><em>.</em><em> gendarussa</em> Burm.f., but these was not effected toward lenght formation time and number of hairy root. The treatment of OD<sub>600</sub> 0.2 was the best treatment for hairy root induction on <em>Justicia gendarussa </em>Burm. f. This data could be used for optimized the quality of methode of hairy root induction. </p>

scholarly journals Advances and Perspectives in Tissue Culture and Genetic Engineering of Cannabis

2021 ◽  
Vol 22 (11) ◽  
pp. 5671
Author(s):  
Mohsen Hesami ◽  
Austin Baiton ◽  
Milad Alizadeh ◽  
Marco Pepe ◽  
Davoud Torkamaneh ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Genetic Engineering ◽  
Suspension Culture ◽  
Secondary Metabolite ◽  
Hairy Root ◽  
Root Culture ◽  
Hairy Root Culture ◽  
Gene Transformation ◽  
Secondary Metabolite Production ◽  
Metabolite Production

For a long time, Cannabis sativa has been used for therapeutic and industrial purposes. Due to its increasing demand in medicine, recreation, and industry, there is a dire need to apply new biotechnological tools to introduce new genotypes with desirable traits and enhanced secondary metabolite production. Micropropagation, conservation, cell suspension culture, hairy root culture, polyploidy manipulation, and Agrobacterium-mediated gene transformation have been studied and used in cannabis. However, some obstacles such as the low rate of transgenic plant regeneration and low efficiency of secondary metabolite production in hairy root culture and cell suspension culture have restricted the application of these approaches in cannabis. In the current review, in vitro culture and genetic engineering methods in cannabis along with other promising techniques such as morphogenic genes, new computational approaches, clustered regularly interspaced short palindromic repeats (CRISPR), CRISPR/Cas9-equipped Agrobacterium-mediated genome editing, and hairy root culture, that can help improve gene transformation and plant regeneration, as well as enhance secondary metabolite production, have been highlighted and discussed.

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