scholarly journals Hemagglutinating and acid phosphatase (AcPASE) activities in developing seedlings of four species of Cucurbitaceae

2014 ◽  
Vol 68 (4) ◽  
pp. 291-297
Author(s):  
Irena Lorenc-Kubis ◽  
Urszula Uram-Walaszczyk
Keyword(s):  
Acid Phosphatase ◽  
Western Blot ◽  
Cucumis Sativus ◽  
Cucurbita Pepo ◽  
Cucurbita Maxima ◽  
Dot Blot ◽  
Con A ◽  
Seeds Germination

The acid phosphatase and hemagglutinating activities of four species of <em>Cucurbitaceae</em> were determined during seeds germination and seedlings development. In all cases traces of enzyme and hemagglutinating activities were found in dry and imbibided seeds. In developing seedlings of <em>Cucumis sativus</em> the activities increased to maximum on the 3rd day while in other species on the 6th day of germination and than fell down. Dot blot and Western blot techniques have shown that in seeds and seedlings of all investigated species present were proteins which cross-reacted with antibodies raised against lectins: CLBa and Con A. It has been shown that proteins from seeds and seedlings of <em>Cucurbita maxima</em> var. <em>bambino</em>, <em>Cucurbita pepo</em> var. <em>giromontia</em> and <em>Cucumis sativus</em> had more pronounced antigenical similarity to lectin CLBa (from <em>Cucurbitaceae</em>) than Con A, while proteins from cotyledons of <em>Cucurbita pepo</em> var. <em>patissonina</em> reacted better with antibodies raised against Con A (the lectin from <em>Papilionaceae</em>) than with CLBa.

scholarly journals Immunologically related lectins from stems and roots of developing seedlings of Cucurbita ficifolia: purification and some properties of root and stem lectins

2014 ◽  
Vol 70 (1) ◽  
pp. 31-37 ◽  
Author(s):  
Irena Lorenc-Kubis ◽  
Agnieszka Łopuska ◽  
Magellan Kalinowska
Keyword(s):  
Western Blot ◽  
Blood Cells ◽  
Gel Filtration ◽  
Seedling Development ◽  
Dot Blot ◽  
Con A ◽  
Vegetative Tissues ◽  
Human Red Blood Cells ◽  
Cucurbita Ficifolia ◽  
Seeds Germination

Hemagglutinating activity has been found in acetate extracts from roots and stems of squash seedlings (<em>Cucurbita ficifolia</em>). The hemaglutinating activity changes during seeds germination and seedling development. Dot blot and Western blot techniques have shown that proteins from these vegetative tissues cross-reacted with antibodies raised against endogenous cotyledons lectin CLBa and Con A.Lectins were isolated from stems and roots of 6-day old seedlings by precipitation with ethanol, affinity chromatography on Con A-Sepharose, gel filtration on Bio-gel P100 and separated by electrophoresis on polyacrylamide gel. Three purified lectins (RLA<sub>1</sub>, RLA<sub>2</sub>, RLA<sub>3</sub>) were obtained from roots and four from stems (SLA<sub>1</sub>, SLA<sub>2</sub>, SLA<sub>3</sub>, SLA<sub>4</sub>). The purified lectins from roots and stems agglutinated all human red blood cells, but sheep erythrocytes were most sensitive to agglutination. The hemagglutination of the root lectins RLA<sub>2</sub> and RLA<sub>3</sub> was inhibited by a very low concentration of arabinose, while RLA<sub>1</sub>, of xylose and Ga1NAc. Arabinose and Xylose were also found to be the most effective inhibitors of all stem lectins.

Use of monoclonal and polyclonal antibodies to detect prostatic acid phosphatase by immunoblotting.

1986 ◽  
Vol 32 (10) ◽  
pp. 1832-1835 ◽  
Author(s):  
P C Patel ◽  
L Aubin ◽  
J Côte
Keyword(s):  
Monoclonal Antibodies ◽  
Acid Phosphatase ◽  
Western Blot ◽  
Polyclonal Antibodies ◽  
Prostatic Acid Phosphatase ◽  
The Other ◽  
Dot Blot ◽  
Western Blots ◽  
Dot Blot Assay ◽  
Polyclonal Antisera

Abstract We investigated two techniques of immunoblotting--the Western blot and the dot blot--for use in detecting prostatic acid phosphatase (PAP, EC 3.1.3.2). We used polyclonal antisera to human PAP, produced in rabbits by hyperimmunization with purified PAP, and PAP-specific monoclonal antibodies in the immunoenzymatic protocols. We conclude that PAP can be readily detected by Western blots with use of polyclonal antisera, but not with monoclonal antibodies. On the other hand, using a dot blot assay, we could easily detect PAP with both polyclonal and monoclonal antibodies.

scholarly journals Acid phosphatases in seeds and developing of squash (Cucurbita ficifolia)

2014 ◽  
Vol 63 (1) ◽  
pp. 37-42
Author(s):  
Irena Lorenc-Kubis
Keyword(s):  
Enzyme Activity ◽  
Acid Phosphatase ◽  
Acid Phosphatase Activity ◽  
Antigenic Relationship ◽  
Acid Phosphatases ◽  
Con A ◽  
Affinity Electrophoresis ◽  
Cucurbita Ficifolia ◽  
Monospecific Antibodies ◽  
Seeds Germination

Changes in protein content and acid phosphatase activity were followed during germination (imbition through seedlings development) in extracts from cotyledons of squash (<i>Cucurbita ficifolia</i>). It has been shown that the activity of acid phosphatase was initially low and than increased to a maximum after 6 days of imbition. Acid phosphates were isolated from cotyledons of seeds and from 6-, 10- and 22-days old seedlings by extraction the proteins with 0.1 M acetate buffer pH 5.1, precipitation with ethanol and by affinity chromatography on con A-Sepharose. Two glycoprotein enzymes AcPase Ba and AcPase Bb which differ in their affinity to immobilized con A were obtained. Both acid phosphatates retained the enzyme activity after binding to free con A. Rocket affinity electrophoresis of AcPase Ba and AcPase Bb, isolated from cotyledons of seeds and seedlings, revealed differences in their ability to bind to con A during seeds germination and seedling develop-ment indicating changes in their sugar component. Con A was found to activate both enzymes. The enzymes cross-reacted with monospecific antibodies raised against grass seed acid phosphatate Ba indicating an antigenic relationship between squash and grass acid phosphatases.

Cunninghamella echinulata. [Descriptions of Fungi and Bacteria].

1966 ◽  
Author(s):  
A. K. Sarbhoy
Keyword(s):  
Geographical Distribution ◽  
World Wide ◽  
Cucurbita Pepo ◽  
Cucurbita Maxima ◽  
Tropical Countries ◽  
Cunninghamella Echinulata

Abstract A description is provided for Cunninghamella echinulata. Information is included on the disease caused by the organism, its transmission, geographical distribution, and hosts. HOSTS: On decaying flowers of Cucurbita pepo, dung and soil. DISEASE: Reported pathogenic to Spanish pumpkin (Cucurbita maxima) at Chandigarh and neighbouring villages in the Punjab, India by Grover (1965). Symptoms recognizable when the flowers begin to die off gradually. Diseased flowers do not open properly. Infected flowers are covered with the cottony mycelium of the pathogen and infected fruits may become detached from the plant and continue to rot on the soil. Leaves and stalks are also frequently infected. GEOGRAPHICAL DISTRIBUTION: World-wide (mostly tropical countries). TRANSMISSION: Air borne; may survive in fragments of mummified pumpkin fruit up to 32 months.

scholarly journals Chaves analíticas para determinação de cultivares de aboboreiras, morangueiras e mogangueiras

1980 ◽  
Vol 37 (2) ◽  
pp. 757-761
Author(s):  
Luiz Antonio Rochelle
Keyword(s):  
Cucurbita Pepo ◽  
Cucurbita Moschata ◽  
Cucurbita Maxima

De sete cultivares de aboboreiras rasteiras, pertencentes à espécie Cucurbita moschata Duch., duas morangueiras e duas mogangueiras, da espécie Cucurbita maxima Duch., uma aboboreira nao rasteira e uma morangueira pertencente a Cucurbita pepo L., elaborou-se chaves analíticas para determina-los, fundamentando-se nos caracteres morfológicos do caule e das folhas.

scholarly journals EFEITO DO NÍVEL DE UMEDECIMENTO DO SUBSTRATO SOBRE A GERMINAÇÃO DE CUCURBITACEAS

1993 ◽  
Vol 23 (2) ◽  
pp. 157-160 ◽  
Author(s):  
Nilson Lemos de Menezes ◽  
Terezinha Lucia Denardin da Silveira ◽  
Lindolfo Storck
Keyword(s):  
Cucumis Sativus ◽  
Cucumis Melo ◽  
Cucurbita Pepo

Avaliou-se a germinação e o tamanho inicial das plântulas de pepino (Cucumis sativus), melancia (Citrulius lanatus), melão (Cucumis melo) e mogango (Cucurbita pepo) cujas sementes foram submetidas as condições do teste padrão de germinação, em rolos de papel toalha, umedecidas com as seguintes quantidades de água: 1,0; 1,5; 2,0; 2,5; 3,0 e 3,5 vezes o peso do papel. Os graus de umedecimento que proporcionam as maiores porcentagens de germinação para pepino e melancia são 2,0 a 2,5 vezes o peso do papel. Para o melão os melhores resultados de germinação são conseguidos com graus de umedecimento de 2,0 a 3,0 vezes o peso do papel, enquanto que para o mogango as quantidades de água requeridas são maiores do que para as demais espécies, devido ao maior tamanho de suas sementes. O aumento da quantidade de água no substrato proporciona aumentos no tamanho da parte aérea das Cucurbitaceas, porém o comprimento das raízes pode sofrer reduções variáveis, de acordo com a espécie estudada, quando a umidade está acima da faixa considerada ideal.

Purification and characterization of aspartic proteinases from Cucumis sativus and Cucurbita maxima seeds

2016 ◽  
Author(s):  
Antoni Polanowski ◽  
Tadeusz Wilusz ◽  
Maria K. Kołaczkowska ◽  
Maciej Wieczorek ◽  
Anna Wilimowska-Pelc ◽  
...  
Keyword(s):  
Cucumis Sativus ◽  
Cucurbita Maxima ◽  
Purification And Characterization ◽  
Aspartic Proteinases

scholarly journals Effect of Salinity Stress on Growth and Metabolomic Profiling of Cucumis sativus and Solanum lycopersicum

Plants ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1626
Author(s):  
Ibrahim Bayoumi Abdel-Farid ◽  
Marwa Radawy Marghany ◽  
Mohamed Mahmoud Rowezek ◽  
Mohamed Gabr Sheded
Keyword(s):  
Secondary Metabolites ◽  
Solanum Lycopersicum ◽  
Cucumis Sativus ◽  
Salinity Stress ◽  
Photosynthetic Pigments ◽  
Germination Rate ◽  
Pronounced Increase ◽  
Metabolomic Profiling ◽  
Seeds Germination

Seeds germination and seedlings growth of Cucumis sativus and Solanum lycopersicum were monitored in in vitro and in vivo experiments after application of different concentrations of NaCl (25, 50, 100 and 200 mM). Photosynthetic pigments content and the biochemical responses of C. sativus and S. lycopersicum were assessed. Salinity stress slightly delayed the seeds germination rate and significantly reduced the percentage of germination as well as shoot length under the highest salt concentration (200 mM) in cucumber. Furthermore, root length was decreased significantly in all treatments. Whereas, in tomato, a prominent delay in seeds germination rate, the germination percentage and seedlings growth (shoot and root lengths) were significantly influenced under all concentrations of NaCl. Fresh and dry weights were reduced prominently in tomato compared to cucumber. Photosynthetic pigments content was reduced but with pronounced decreasing in tomato compared to cucumber. Secondary metabolites profiling in both plants under stress was varied from tomato to cucumber. The content of saponins, proline and total antioxidant capacity was reduced more prominently in tomato as compared to cucumber. On the other hand, the content of phenolics and flavonoids was increased in both plants with pronounced increase in tomato particularly under the highest level of salinity stress. The metabolomic profiling in stressful plants was significantly influenced by salinity stress and some bioactive secondary metabolites was enhanced in both cucumber and tomato plants. The enhancement of secondary metabolites under salinity stress may explain the tolerance and sensitivity of cucumber and tomato under salinity stress. The metabolomic evaluation combined with multivariate data analysis revealed a similar mechanism of action of plants to mediate stress, with variant level of this response in both plant species. Based on these results, the effect of salinity stress on seeds germination, seedlings growth and metabolomic content of plants was discussed in terms of tolerance and sensitivity of plants to salinity stress.

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