scholarly journals Comparative chromosome and molecular studies of some species of genus Arum from eastern Slavonia and Baranya region in Croatia

2011 ◽  
Vol 75 (4) ◽  
pp. 285-292 ◽  
Author(s):  
Mirjana Sabo ◽  
Marija Bedalov ◽  
Anita Lendel ◽  
Tomislav Bačić ◽  
Ljiljana Kristin ◽  
...  

Karyological and molecular studies were done in this paper on three species of genus Arum; <em>Arum italicum</em> Mill. and <em>Arum maculatum</em> L., with two varieties, and <em>Arum alpinum</em> Schott and Kotschy, also with two varieties. The main goal of this paper was to establish whether they were regularly determined exclusively on the principle of morphological parameters. Karyological studies showed that the number of chromosomes for<em> Arum italicum</em> Mill. amounted to 2n=84, for <em>Arum maculatum</em> L. 2n=56 and for <em>Arum alpinum</em> Schott and Kotschy 2n=28. This confirmed that these species are not only clean and separated, but also support the regularity of the morphological determination. Molecular studies, e.g. RAPD method showed that two genetically separated species groups correspond to the three mentioned species. <em>Arum italicum</em> Mill. is the least homogenous species closely related and the variability between populations is high. <em>Arum maculatum</em> L. is more homogenous within the species, two varieties could be differentiated whereas they are closely genetically related and the variability between the populations is too high. <em>Arum alpinum</em> is strongly homogenous and within these species two varieties could also be differentiated. That means that they are closely related and the variability between the populations is very high. These observations mostly coincided with previous morphological investigations.

2020 ◽  
pp. 147-152
Author(s):  
Rainer W. Bussmann ◽  
Ketevan Batsatsashvili ◽  
Zaal Kikvidze ◽  
Narel Y. Paniagua-Zambrana ◽  
Manana Khutsishvili ◽  
...  
Keyword(s):  

1976 ◽  
Vol 24 (2) ◽  
pp. 249 ◽  
Author(s):  
PB Bridgewater

Distribution data for vascular plants in Victoria were recorded on grid squares of 1° latitude by 1.5° longitude. Data for six angiosperm families (Cyperaceae, Liliaceae, Chenopodiaceae, Rhamnaceae, Rutaceae and Myrtaceae) were analysed by the techniques of normal and inverse association analysis. Analyses of the data produced a series of species groups with similar geographical ranges (elements). These elements may be broadly divided into eastern and western groups, with two exceptions-one extending over the southern half of the State and the other occupying the coastal fringe. Geographical elements are helpful in defining the range of plant communities, as well as suggesting hypotheses as to the origins of floras. South central Victoria is seen to be a junction for species of both the eastern and western groups of elements, which may explain the very high number of species recorded from the Melbourne region.


Biologia ◽  
2007 ◽  
Vol 62 (4) ◽  
Author(s):  
Mirjana Sabo ◽  
Tihana Marček ◽  
Tomislav Bačić ◽  
Ljiljana Krstin ◽  
Anita Lendel

AbstractTypes and the number of stomata in the following Arum species: Arum italicum Mill., Arum maculatum var. maculatum L. and Arum maculatum var. immaculatum L., Arum alpinum var. pannonicum Terpo. and Arum alpinum var. intermedium Schur. in three different locations in Zablaće, Normanci and Bilje were investigated. The most prevalent stomata type at both upper and lower epidermis for each Arum species was mostly stomata type paracytic, followed by hexacytic, tetracytic or brachyparacytic as far as locality is concerned. Helicocytic type was more prevalent in Arum alpinum var. pannonicum Terpo. and Arum alpinum var. intermedium Schur. at Bilje. Other stomata types were very rare. A striking regularity in the occurrence of stomata types was not found within a single species. Some stomata types, however, were found either at adaxial or abaxial epidermis or were not present at all. The number of stomata per square mm varied from 25 to 651. A statistically significant difference in the number of stomata per square mm at upper and lower epidermis among Arum species was determined in locations Zablaće and Normanci, whereas no statistically significant differences were found in location Bilje.


2021 ◽  
Vol 17 (2) ◽  
pp. 107-118
Author(s):  
Boychuk S.V. ◽  
Budzhak V.V.

The ontogenetic and vitality structure of eight Muscari botryoides populations from Ukraine have been studied. M. botryoides is a rare, endemic species of the Asparagaceae family, distributed on northeastern limit of its natural range and listed in the 3rd edition of the Red Data Book of Ukraine with conservation status "endangered". The characteristics of the species main ontogenetic states have been given. Age spectra of populations have been constructed. Ontogenetic indices have been calculated according to I.M. Kovalenko. Individuals of eight age states have been identified: seeds (se), seedlings (p), juvenile (j), immature (im), virginal (v), young generative (g1), mature generative (g2) and old generative (g3). Subsenile (ss) and senile (s) individuals were not registered. The populations are normal, noncomplete with left-biased age spectra. Juveniles prevail in all age spectra. . According to A.A. Uranov and O.V. Smirnova all populations are normal young, according to the Delta-Omega classification proposed by L.A. Zhivotovskij, they are young, and according to L.A. Zhukova and T.A. Polyanskaya – perspective. Innovation indices of all populations are very high, and generative, senilis, age indices are low. A factor analysis based on 17 morphological parameters of M. botryoides have been made. The results of the analysis showed, that the following parameters determine vitality of M. botryoides individuals: first leaf length, bulb width, inflorescence length and number of flowers in the inflorescence. Medium vitality individuals dominate in most populations. According to the vitality structure, six populations are prosperous, and two – depressed. Key words: endemic species, age spectrum, ontogenetic indices, vitality, natural population. Досліджено онтогенетичну та віталітетну структуру восьми популяцій Muscari botryoides з території України. M. botryoides – це рідкісний, ендемічний вид з родини Аsparagaceae, який перебуває на північно-східній межі ареалу та занесений до третього видання «Червоної книги України» зі статусом «зникаючий». Подано характеристику основних онтогенетичних станів виду. Побудовано вікові спектри популяцій. Розраховано онтогнетичні індекси за І.М. Коваленко. У складі досліджуваних популяцій M. botryoides виявлено особини восьми вікових станів: насіння (sm), проростки (p), ювенільні (j), іматурні (im), віргінільні (v), молоді генеративні (g1), зрілі генеративні (g2) та старі генеративні (g3). Субсенільні (ss) та сенільні (s) особини не зареєстровані. Вікові спектри всіх досліджуваних популяцій лівосторонні з переважанням рослин ювенільного онтогенетичного стану. За ознаками онтогенетичної структури усі досліджувані популяції – нормальні, неповночленні. Згідно з класифікацією популяцій О.О. Уранова та О.В. Смірнової вони є нормальними молодими, згідно з класифікацією «Дельта-Омега», запропонованою Л.А. Животовським – молодими, згідно з Л.О. Жуковою та Т.А. Полянською – перспективними. Індекси відновлення популяцій дуже високі, а індекси генеративності, старіння та віковості – низькі. Проведено факторний аналіз з урахуванням 17 морфопараметрів M. botryoides, встановлено, що ознаками, які детермінують життєвість особин є: довжина першого листка, ширина цибулини, довжина суцвіття та кількість квіток у суцвітті. Віталітетний аналіз засвідчив, що у більшості досліджуваних популяцій домінують особин середнього рівня життєвості. За віталітетною структурою шість з восьми популяцій відповідають процвітаючому якісному типу, дві популяції перебувають у депресивному стані. Ключові слова: ендемічний вид, віковий спектр, онтогенетичні індекси, життєвість, природна популяція.


HortScience ◽  
1997 ◽  
Vol 32 (3) ◽  
pp. 482E-482
Author(s):  
Hak-Tae Lim

The phylogenetic relationships between Korean endemic, Hanabusaya asiatica, and its allied groups, including four genera and nine species, were investigated at the DNA level using randomly amplified polymorphic DNA (RAPD) method. Ten primers out of 80 primers (10-mer) screened gave rise to very high polymorphism (99%) in all of the tested plants, producing 153 randomly amplified DNA fragments. H. asiatica was differentiated from its allied groups at the 0.62 of similarity index of RAPDs. This results were in accordance with previous classification based on palynological studies. It was confirmed that H. asiatica could be placed into Korean endemic and suggested that RAPD technique be used as an additional method of phylogenetic relationship for plant systematics.


Zootaxa ◽  
2008 ◽  
Vol 1931 (1) ◽  
pp. 25-36 ◽  
Author(s):  
JAN ČERVENKA ◽  
LUKÁŠ KRATOCHVÍL ◽  
DANIEL FRYNTA

Representatives of the gekkonid genus Cyrtopodion and related genera form a highly diversified group of Palearctic geckos, whose taxonomy is still unstable and phylogeny little known. This study tries to improve this situation by attempting to reconstruct the phylogeny based on two molecular markers (partial sequences of mitochondrial cytochrome b and 12S rRNA genes) in the Middle Eastern species of the genus Cyrtopodion and members of the genera Agamura and Bunopus. The results support the monophyly of the species groups within the genus Cyrtopodion as defined by Anderson (1999), but relationships between these groups remain largely unresolved. The kotschyi group (= subgenus Mediodactylus) is not closely related to the rest of the genus Cyrtopodion and should be treated as an autonomous genus. Agamura persica forms a monophyletic group with agamuroides + caspium + scabrum species groups. The genus Bunopus does not seem to be closely related to this clade. We briefly discuss the implications of our findings and stress the importance of further molecular studies in Palearctic geckos.


1989 ◽  
Vol 10 (3) ◽  
pp. 267-275 ◽  
Author(s):  
Timothy F. Sharbel ◽  
David M. Green ◽  

AbstractThe genetic relationships of four species of frogs, genus Rana, from Yunnan, China, were investigated using starch gel electrophoresis of isozymes. Rana chaochiaoensis, R. pleuraden, R. phrynoides and R. limnocharis, representing four different species groups, were compared to each other and to representative species from other parts of Asia: R. amurensis from eastern Siberia, R. japonica from Japan, and R. nigromaculata from Korea. Twenty-four isozyme loci were reliably detected and scored, of which all but one were variable. R. chaochiaoensis, R. japonica and R. amurensis, all members of the Eurasian "brown frog" group, clustered together as a group although genetic distances were comparatively high. The "pond frogs", R. nigromaculata and R. pleuraden, similarly clustered together. Both R. phrynoides and R. limnocharis were highly divergent from the other species, but R. phrynoides seemed to be more closely affiliated with the "pond frogs" than with the "brown frogs". The data indicate that the highly enigmatic 64-chromosome karyotype of R. phrynoides arose from among 26-chromosome ancestors. The relationships of R. limnocharis cannot be precisely defined. Genetic divergence among Asiatic Rana appears to be very high.


1994 ◽  
Vol 144 ◽  
pp. 593-596
Author(s):  
O. Bouchard ◽  
S. Koutchmy ◽  
L. November ◽  
J.-C. Vial ◽  
J. B. Zirker

AbstractWe present the results of the analysis of a movie taken over a small field of view in the intermediate corona at a spatial resolution of 0.5“, a temporal resolution of 1 s and a spectral passband of 7 nm. These CCD observations were made at the prime focus of the 3.6 m aperture CFHT telescope during the 1991 total solar eclipse.


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