scholarly journals Supercritical carbon dioxide Cannabis sativa L. and Humulus lupulus extracts and their influence on human macrophages inflammatory state*

2019 ◽  
Vol 73 ◽  
pp. 782-790
Author(s):  
Aleksandra Gregorius ◽  
Wojciech Krzyczkowski ◽  
Marta Wierucka ◽  
Julia Kupińska ◽  
Agnieszka Dębczak ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Inflammatory Cytokines ◽  
Cannabis Sativa ◽  
Humulus Lupulus ◽  
High Concentration ◽  
Inflammatory State ◽  
Pro Inflammatory Cytokines ◽  
Supercritical Carbon

Herbal extracts are promising immunomodulating compounds. Their standardization may improve clinical outcome in various conditions related to inflammatory state. The aim of this study was to assess the utility of Cannabis sativa L. and Humulus lupulus extracts obtained by supercritical carbon dioxide (scCO2) in the reduction of pro-inflammatory cytokines release after LPS stimulation in the in vitro model. After scCO2 extraction, the cytotoxic potential of the obtained compounds was determined. The highest non-cytotoxic concentrations were selected for further inflammatory testing. PMA-differentiated U937 cells were used as an LPS induced model of the inflammation to assess the extracts potential to decrease the level of the pro-inflammatory cytokines IL-1β, IL-6 and TNF-α. Either individually tested or in combination scCO2 extracts markedly reduced the level of released pro-inflammatory cytokines in comparison to LPS stimulated positive control. Our results show that the usage of standardized Cannabis sativa L. and Humulus lupulus extracts might be beneficial in reducing the inflammatory state. Application of the mixed extracts not only reduces the need for a high concentration of pure compounds, but also broadens the possible therapeutic effect. Moreover, scCO2 extraction may serve as the efficient method of obtaining functional anti-inflammatory extracts from either hop cones or cannabis.

Processing of coked Pt-Re/γ-Al2O3 catalysts with high-concentration ozone dissolved in supercritical carbon dioxide

2010 ◽  
Vol 4 (8) ◽  
pp. 1217-1227 ◽  
Author(s):  
S. N. Gaydamaka ◽  
V. V. Timofeev ◽  
Yu. V. Guryev ◽  
D. A. Lemenovskiy ◽  
G. P. Brusova ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
High Concentration ◽  
Supercritical Carbon

scholarly journals Experimental Data and Modelling of the Solubility of High-Carotenoid Paprika Extract in Supercritical Carbon Dioxide

Molecules ◽  
2019 ◽  
Vol 24 (22) ◽  
pp. 4174 ◽  
Author(s):  
Dorota Kostrzewa ◽  
Agnieszka Dobrzyńska-Inger ◽  
August Turczyn
Keyword(s):  
Experimental Data ◽  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Static Method ◽  
Coefficient Of Determination ◽  
High Concentration ◽  
Relative Deviation ◽  
Measurement Results ◽  
Supercritical Carbon

The studies of solubility of the paprika extract with a high concentration of carotenoids in carbon dioxide under the pressure of 20–50 MPa and at temperatures of 313.15–333.15 K were carried out using the static method. The highest solubility of paprika extract was achieved at the temperature of 333.15 K and under the pressure of 50 MPa. The obtained experimental data were correlated with five density-based models, applied for prediction of solubility in the supercritical carbon dioxide (the Chrastil, del Valle and Aguilera, Adachi and Lu, Sparks et al. and Bian et al. models). The accuracy of particular models with reference to measurement results was specified with the average absolute relative deviation (AARD) and coefficient of determination (R2). Results showed that solubility calculated based on the selected models was compliant with experimental data.

scholarly journals Bioaccessibility of Antioxidants in Blackcurrant Juice After Treatment Using Supercritical Carbon Dioxide

2021 ◽  
Author(s):  
Urszula Trych ◽  
Magdalena Buniowska ◽  
Sylwia Skąpska ◽  
Ireneusz Kapusta ◽  
Krystian Marszałek
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Vitamin C ◽  
Model Experiment ◽  
Dehydroascorbic Acid ◽  
Total Anthocyanins ◽  
The Stability ◽  
Supercritical Carbon ◽  
Thermally Treated

Blackcurrant juice (Ribes nigrum L.) was subjected to supercritical carbon dioxide (SCCD) at 10, 30 and 60 MPa for 10 min at 45°C as well as thermally treated at 45°C and 85°C for 10 min to determine the stability, antioxidant capacity (AC) and bioaccessibility (BAc) of vitamin C, total anthocyanins and their individual monomers. An in vitro gastrointestinal digestion model completed with dialysis was used to assess BAc. The use of SCCD at each of the pressures applied improved the stability of vitamin C, total anthocyanins, and AC before in vitro digestion. As a result of digestion, L-ascorbic acid was oxidized to L-dehydroascorbic acid, and finally, the total content of vitamin C, anthocyanins, and AC decreased. SCCD did not significantly improve the BAc of vitamin C and total anthocyanins. The highest BAc of vitamin C was noted in fresh juice (FJ) (40%) and after mild heat treatment at 45°C (T45) (46%). The highest BAc of total anthocyanins was also noted in the FJ (4.4%). The positive effect of the application of SCCD on the BAc of the delphinidin-3-O-glycosides was observed compared to T45 and thermal pasteurization at 85°C (T85). Moreover, cyanidins were generally more bioaccessible than delphinidins in all samples. AC after digestion was higher in SCCD samples compared to thermally treated measured using ABTS+• and DPPH• assays, whereas in dialysate similar trends were observed only for AC measured using the ABTS+• assay. This phenomenon was justified by the formation of individual metabolites detected by UPLC-PDA-MS / MS in the model experiment with delphinidin-3-O-rutinoside. The protocatechuic acid which is well known as a strong antioxidant was detected in the model experiment after digestion. Further research is needed to better understand the metabolic pathway of anthocyanins and the possible uses of SCCD to improve the health properties of fruit products.

In vitro release profiles of β-carotene encapsulated in PHBV by means of supercritical carbon dioxide micronization technique

2011 ◽  
Vol 56 (2) ◽  
pp. 137-143 ◽  
Author(s):  
Wagner L. Priamo ◽  
Alana M. de Cezaro ◽  
Stéphani C. Benetti ◽  
J. Vladimir Oliveira ◽  
Sandra R.S. Ferreira
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
In Vitro Release ◽  
Vitro Release ◽  
Β Carotene ◽  
Release Profiles ◽  
Supercritical Carbon
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