Preparation and in vitro/in vivo Evaluation of Lacidipine by Adsorption onto Fumed Silica Using Supercritical Carbon Dioxide

2016 ◽  
Vol 13 (7) ◽  
pp. 1053-1064 ◽  
Author(s):  
Yajie Geng ◽  
Qiang Fu ◽  
Bei Guo ◽  
Yun Li ◽  
Xiangrong Zhang ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Fumed Silica ◽  
In Vivo Evaluation ◽  
Supercritical Carbon

scholarly journals Isolation, Development, and Genomic Analysis ofBacillus megateriumSR7 for Growth and Metabolite Production Under Supercritical Carbon Dioxide

2018 ◽  
Author(s):  
Adam J.E. Freedman ◽  
Kyle C. Peet ◽  
Jason T. Boock ◽  
Kevin Penn ◽  
Kristala L. J. Prather ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Enrichment Culture ◽  
Aqueous Media ◽  
Organic Substrates ◽  
Chemical Induction ◽  
Diverse Range ◽  
Supercritical Carbon

AbstractSupercritical carbon dioxide (scCO2) is an attractive substitute for conventional organic solvents due to its unique transport and thermodynamic properties, its renewability and labile nature, and its high solubility for compounds such as alcohols, ketones and aldehydes. However, biological systems that use scCO2are mainly limited toin vitroprocesses due to its strong inhibition of cell viability and growth. To solve this problem, we used a bioprospecting approach to isolate a microbial strain with the natural ability to grow while exposed to scCO2. Enrichment culture and serial passaging of deep subsurface fluids from the McElmo Dome scCO2reservoir in aqueous media under scCO2headspace enabled the isolation of spore-forming strainBacillus megateriumSR7. Sequencing and analysis of the complete 5.51 Mbp genome and physiological characterization revealed the capacity for facultative anaerobic metabolism, including fermentative growth on a diverse range of organic substrates. Supplementation of growth medium withL-alanine for chemical induction of spore germination significantly improved growth frequencies and biomass accumulation under scCO2headspace. Detection of endogenous fermentative compounds in cultures grown under scCO2represents the first observation of bioproduct generation and accumulation under this condition. Culturing development and metabolic characterization ofB. megateriumSR7 represent initial advancements in the effort towards enabling exploitation of scCO2as a sustainable solvent forin vivobioprocessing.

scholarly journals Bioaccessibility of Antioxidants in Blackcurrant Juice After Treatment Using Supercritical Carbon Dioxide

2021 ◽  
Author(s):  
Urszula Trych ◽  
Magdalena Buniowska ◽  
Sylwia Skąpska ◽  
Ireneusz Kapusta ◽  
Krystian Marszałek
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Vitamin C ◽  
Model Experiment ◽  
Dehydroascorbic Acid ◽  
Total Anthocyanins ◽  
The Stability ◽  
Supercritical Carbon ◽  
Thermally Treated

Blackcurrant juice (Ribes nigrum L.) was subjected to supercritical carbon dioxide (SCCD) at 10, 30 and 60 MPa for 10 min at 45°C as well as thermally treated at 45°C and 85°C for 10 min to determine the stability, antioxidant capacity (AC) and bioaccessibility (BAc) of vitamin C, total anthocyanins and their individual monomers. An in vitro gastrointestinal digestion model completed with dialysis was used to assess BAc. The use of SCCD at each of the pressures applied improved the stability of vitamin C, total anthocyanins, and AC before in vitro digestion. As a result of digestion, L-ascorbic acid was oxidized to L-dehydroascorbic acid, and finally, the total content of vitamin C, anthocyanins, and AC decreased. SCCD did not significantly improve the BAc of vitamin C and total anthocyanins. The highest BAc of vitamin C was noted in fresh juice (FJ) (40%) and after mild heat treatment at 45°C (T45) (46%). The highest BAc of total anthocyanins was also noted in the FJ (4.4%). The positive effect of the application of SCCD on the BAc of the delphinidin-3-O-glycosides was observed compared to T45 and thermal pasteurization at 85°C (T85). Moreover, cyanidins were generally more bioaccessible than delphinidins in all samples. AC after digestion was higher in SCCD samples compared to thermally treated measured using ABTS+• and DPPH• assays, whereas in dialysate similar trends were observed only for AC measured using the ABTS+• assay. This phenomenon was justified by the formation of individual metabolites detected by UPLC-PDA-MS / MS in the model experiment with delphinidin-3-O-rutinoside. The protocatechuic acid which is well known as a strong antioxidant was detected in the model experiment after digestion. Further research is needed to better understand the metabolic pathway of anthocyanins and the possible uses of SCCD to improve the health properties of fruit products.

In vitro release profiles of β-carotene encapsulated in PHBV by means of supercritical carbon dioxide micronization technique

2011 ◽  
Vol 56 (2) ◽  
pp. 137-143 ◽  
Author(s):  
Wagner L. Priamo ◽  
Alana M. de Cezaro ◽  
Stéphani C. Benetti ◽  
J. Vladimir Oliveira ◽  
Sandra R.S. Ferreira
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
In Vitro Release ◽  
Vitro Release ◽  
Β Carotene ◽  
Release Profiles ◽  
Supercritical Carbon

scholarly journals Supercritical carbon dioxide Cannabis sativa L. and Humulus lupulus extracts and their influence on human macrophages inflammatory state*

2019 ◽  
Vol 73 ◽  
pp. 782-790
Author(s):  
Aleksandra Gregorius ◽  
Wojciech Krzyczkowski ◽  
Marta Wierucka ◽  
Julia Kupińska ◽  
Agnieszka Dębczak ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Supercritical Carbon Dioxide ◽  
Inflammatory Cytokines ◽  
Cannabis Sativa ◽  
Humulus Lupulus ◽  
High Concentration ◽  
Inflammatory State ◽  
Pro Inflammatory Cytokines ◽  
Supercritical Carbon

Herbal extracts are promising immunomodulating compounds. Their standardization may improve clinical outcome in various conditions related to inflammatory state. The aim of this study was to assess the utility of Cannabis sativa L. and Humulus lupulus extracts obtained by supercritical carbon dioxide (scCO2) in the reduction of pro-inflammatory cytokines release after LPS stimulation in the in vitro model. After scCO2 extraction, the cytotoxic potential of the obtained compounds was determined. The highest non-cytotoxic concentrations were selected for further inflammatory testing. PMA-differentiated U937 cells were used as an LPS induced model of the inflammation to assess the extracts potential to decrease the level of the pro-inflammatory cytokines IL-1β, IL-6 and TNF-α. Either individually tested or in combination scCO2 extracts markedly reduced the level of released pro-inflammatory cytokines in comparison to LPS stimulated positive control. Our results show that the usage of standardized Cannabis sativa L. and Humulus lupulus extracts might be beneficial in reducing the inflammatory state. Application of the mixed extracts not only reduces the need for a high concentration of pure compounds, but also broadens the possible therapeutic effect. Moreover, scCO2 extraction may serve as the efficient method of obtaining functional anti-inflammatory extracts from either hop cones or cannabis.

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