scholarly journals Dose dependence and durability of the therapeutic effects of Asparagus cochinchinensis fermented extract in an ovalbumin-challenged asthma model

2018 ◽  
Vol 34 (3) ◽  
pp. 101 ◽  
Author(s):  
Jun Young Choi ◽  
Ji Won Park ◽  
Ji Eun Kim ◽  
Jin Ju Park ◽  
Mi Rim Lee ◽  
...  
Keyword(s):  
Dose Dependence ◽  
Therapeutic Effects ◽  
Asthma Model ◽  
Asparagus Cochinchinensis

scholarly journals The Anti-Inflammatory Effects of Fermented Herbal Roots of Asparagus cochinchinensis in an Ovalbumin-Induced Asthma Model

2018 ◽  
Vol 7 (10) ◽  
pp. 377 ◽  
Author(s):  
Jun Choi ◽  
Ji Kim ◽  
Jin Park ◽  
Mi Lee ◽  
Bo Song ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Airway Remodeling ◽  
Inflammatory Cells ◽  
Treated Group ◽  
Therapeutic Effects ◽  
Molecular Response ◽  
Asthma Model ◽  
Cholinergic Regulation ◽  
Asparagus Cochinchinensis ◽  
Pharmacologically Active

Introduction: Roots of Asparagus cochinchinensis, which have pharmacologically active ingredients, have received great attention because they show good therapeutic effects for various inflammatory diseases without specific toxicity. This study investigated the anti-asthmatic effects of a butanol extract of Asparagus cochinchinensis roots that had been fermented with Weissella cibaria (BAW) and its possible underlying cholinergic regulation. Methods: Alterations of the anti-asthmatic markers and the molecular response factors were measured in an ovalbumin (OVA)-induced asthma model after treatment with BAW. Results: Treatment with BAW decreased the intracellular reactive oxygen species (ROS) production in lipopolysaccharides (LPS) activated RAW264.7 cells. The results of the animal experiments revealed lower infiltration of inflammatory cells and bronchial thickness, and a significant reduction in the number of macrophages and eosinophils, concentration of OVA-specific IgE, and expression of Th2 cytokines in the OVA + BAW treated group. In addition, a significant recovery of goblet cell hyperplasia, MMP-9 expression, and the VEGF signaling pathway was observed upon airway remodeling in the OVA + BAW treated group. Furthermore, these responses of BAW were linked to recovery of acetylcholine esterase (AChE) activity and muscarinic acetylcholine receptor (mAChR) M3 downstream signaling pathway in epithelial cells, smooth muscle cells, and afferent sensory nerves of OVA + BAW-treated mice. Conclusion: Overall, these findings are the first to provide evidence that the therapeutic effects of BAW can prevent airway inflammation and remodeling through the recovery of cholinergic regulation in structural cells and inflammatory cells of the chronic asthma model.

scholarly journals Therapeutic effects of histone deacetylase inhibitors in a murine asthma model

2016 ◽  
Vol 65 (12) ◽  
pp. 995-1008 ◽  
Author(s):  
Yuan Ren ◽  
Xinming Su ◽  
Lingfei Kong ◽  
Menglu Li ◽  
Xuan Zhao ◽  
...  
Keyword(s):  
Histone Deacetylase ◽  
Histone Deacetylase Inhibitors ◽  
Therapeutic Effects ◽  
Asthma Model ◽  
Murine Asthma Model

Therapeutic effects of anti-B7-1 antibody in an ovalbumin-induced mouse asthma model

2008 ◽  
Vol 8 (9) ◽  
pp. 1190-1195 ◽  
Author(s):  
Wei Ji ◽  
Xuyan Chen ◽  
Chen Zhengrong ◽  
Hu Yumin ◽  
Li Huang ◽  
...  
Keyword(s):  
Therapeutic Effects ◽  
Asthma Model

scholarly journals Saponin-enriched extract of Asparagus cochinchinensis alleviates airway inflammation and remodeling in ovalbumin-induced asthma model

2017 ◽  
Vol 40 (5) ◽  
pp. 1365-1376 ◽  
Author(s):  
Ji-Eun Sung ◽  
Hyun-Ah Lee ◽  
Ji-Eun Kim ◽  
Woo-Bin Yun ◽  
Beum-Soo An ◽  
...  
Keyword(s):  
Airway Inflammation ◽  
Asthma Model ◽  
Asparagus Cochinchinensis

scholarly journals Four amino acids as serum biomarkers for anti-asthma effects in the ovalbumin-induced asthma mouse model treated with extract of Asparagus cochinchinensis

2019 ◽  
Vol 35 (1) ◽  
Author(s):  
Jun Young Choi ◽  
So Hyun Kim ◽  
Ji Eun Kim ◽  
Ji Won Park ◽  
Mi Ju Kang ◽  
...  
Keyword(s):  
Amino Acids ◽  
Profile Analysis ◽  
Inflammatory Cells ◽  
Treated Group ◽  
High Concentration ◽  
Butanol Extract ◽  
Asthma Model ◽  
Asparagus Cochinchinensis ◽  
Endogenous Metabolites ◽  
Vehicle Treated Group

AbstractThe butanol extract of Asparagus cochinchinensis roots fermented with Weissella cibaria (BAW) effectively prevents inflammation and remodeling of airway in the ovalbumin (OVA)-induced asthma model. To characterize biomarkers that can predict the anti-asthmatic effects induced by BAW treatment, we measured the alteration of endogenous metabolites in the serum of OVA-induced asthma mice after administration of low concentration BAW (BAWLo, 250 mg/kg) and high concentration BAW (BAWHi, 500 mg/kg) using 1H nuclear magnetic resonance (1H-NMR) spectral data. The number of immune cells and serum concentration of IgE as well as thickness of the respiratory epithelium and infiltration of inflammatory cells in the airway significantly recovered in the OVA+BAW treated group as compared to the OVA+Vehicle treated group. In the metabolic profile analysis, the pattern recognition showed completely separate clustering of serum analysis parameters between the OVA+Vehicle and OVA+BAW treated groups. Of the total endogenous metabolites, 19 metabolites were upregulated or downregulated in the OVA+Vehicle treated group as compared to the Control treated group. However, only 4 amino acids (alanine, glycine, methionine and tryptophan) were significantly recovered after BAWLo and BAWHi treatment. This study provides the first results pertaining to metabolic changes in the asthma model mice treated with OVA+BAW. Additionally, these findings show that 4 metabolites can be used as one of biomarkers to predict the anti-asthmatic effects.

scholarly journals Anti-Inflammatory Response and Muscarinic Cholinergic Regulation during the Laxative Effect of Asparagus cochinchinensis in Loperamide-Induced Constipation of SD Rats

2019 ◽  
Vol 20 (4) ◽  
pp. 946 ◽  
Author(s):  
Ji Kim ◽  
Ji Park ◽  
Mi Kang ◽  
Hyeon Choi ◽  
Su Bae ◽  
...  
Keyword(s):  
Inflammatory Response ◽  
Inflammatory Responses ◽  
Gastrointestinal Transit ◽  
Treated Group ◽  
Therapeutic Effects ◽  
Sd Rats ◽  
Cholinergic Regulation ◽  
Muscarinic Cholinergic ◽  
Anti Inflammatory ◽  
Asparagus Cochinchinensis

Several types of saponins and herbal plants containing saponins have been reported to have anti-inflammatory or laxative activities. To verify the therapeutic effects of saponin-enriched extracts of Asparagus cochinchinensis (SPA) on the anti-inflammatory response and on the cholinergic regulation in the gastrointestinal system, an alteration on the constipation phenotypes, on the inflammatory responses, and on the muscarinic cholinergic regulation were investigated in the transverse colons of Sprague Dawley (SD) rats with loperamide (Lop)-induced constipation after the treatment of SPA. Significant increases were observed on the total number of stools, the gastrointestinal transit, the thickness of the mucosal layer, the flat luminal surface, the number of paneth cells, and the lipid droplets in the Lop + SPA-treated group as compared to the Lop + Vehicle-treated group. SPA treatment induced the recovery of inflammatory cytokines (TNF-α, IL-1β) and IL-6), inflammatory mediators (NF-κB and iNOS), the total number of infiltered mast cells, and mucin secretion. Also, some similar improvements were observed on the levels of acetylcholine esterase (AChE) activity and on the phosphorylation of myosin light chains (MLC) as well as the expression of muscarinic acetylcholine receptors M2/M3 (mAChR M2/M3) and their mediators. The results presented herein provide the first strong evidence that SPA stimulates anti-inflammatory responses and the muscarinic cholinergic regulation when exerting its laxative effects in the chronic constipation of Lop-induced models.

scholarly journals Acupuncture Has a Positive Effect on Asthmatic Rats in a Glucocorticoid-Independent Manner

2016 ◽  
Vol 34 (6) ◽  
pp. 433-440 ◽  
Author(s):  
Wen-Qian Wang ◽  
Yu-Dong Xu ◽  
Long-Ping Cui ◽  
Lei-Miao Yin ◽  
Yu Wang ◽  
...  
Keyword(s):  
Eosinophil Count ◽  
Alternative Therapy ◽  
Serum Cortisol ◽  
Therapeutic Effects ◽  
Dependent Manner ◽  
Independent Manner ◽  
Beneficial Effects ◽  
Asthma Model ◽  
Positive Effect

Background There is some evidence to support the use of acupuncture as an alternative therapy for asthma. However, the mechanisms underlying its effects are not fully understood. We have reported previously that acupuncture has beneficial effects on asthma without changing the concentration of serum cortisol, although endogenous glucocorticoid (GC) plays an important role in regulating immune responses. Objective In this study, bilateral adrenalectomy (removal of both adrenal glands) was performed in rats before asthma model induction to investigate whether acupuncture influences asthma in a GC-dependent manner. Methods Adrenal-intact and adrenalectomised rats were injected with ovalbumin to induce asthma and then left untreated or treated with manual acupuncture (MA) at GV14, bilateral BL12 and bilateral BL13, or manual restraint without MA. Healthy and sham-adrenalectomised control groups were also included. Pulmonary resistance (RL), serum concentrations of corticosterone, and eosinophil counts were measured at the end of the experimental course. Sera from adrenal-intact and adrenalectomised asthmatic rats treated with acupuncture were injected into untreated adrenal-intact and adrenalectomised asthmatic rats to investigate further the potential role of GC in the effect of acupuncture. Results Acupuncture significantly decreased RL and eosinophil count in both adrenal-intact and adrenalectomised asthmatic rats. Moreover, administration of sera derived from acupuncture-treated adrenal-intact and adrenalectomised asthmatic rats attenuated the increase in RL and eosinophil count in both asthmatic models. Conclusions Results of this study suggest that endogenous GC is not a key contributor to the effects of acupuncture on asthma, and that acupuncture may have potentially therapeutic effects on asthma in a GC-independent manner.

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