Anti-inflammatory, Antinociceptive Activity of an Essential Oil Recipe Consisting of the Supercritical Fluid CO2 Extract of White Pepper, Long Pepper, Cinnamon, Saffron and Myrrh in vivo

Several studies have investigated the antinociceptive, immunomodulatory and anti-inflammatory properties of compounds found in the lavender essential oil (LEO), however to date, there is still lack of substantial data. The objective of this study was to assess the antioxidant, anti-inflammatory and antinociceptive effects of lavender essential oil. The 1,1-diphenyl-2-picrylhydrazyl radical decolorization assay was used for antioxidant activity evaluation. The anti-inflammatory activity was tested using two models of acute inflammation: carrageenan-induced pleurisy and croton oil-induced ear edema. The antinociceptive activity was tested using the pain model induced by formalin. LEO has antioxidant activity, which is dose-dependent response. The inflammatory response evoked by carrageenan and by croton oil was reduced through the pre-treatment of animals with LEO. In the pleurisy model, the drug used as positive control, dexamethasone, was more efficacious. However, in the ear swelling, the antiedematogenic effect of the oil was similar to that observed for dexamethasone. In the formalin test, LEO consistently inhibited spontaneous nociception and presented a similar effect to that of tramadol. The results of this study reveal (in vivo) the analgesic and anti-inflammatory activities of LEO and demonstrates its important therapeutic potential.

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The anti-inflammatory potential of Cinnamomum camphora (L.) J.Presl essential oil in vitro and in vivo

Journal of Ethnopharmacology ◽

10.1016/j.jep.2020.113516 ◽

2021 ◽

Vol 267 ◽

pp. 113516

Author(s):

Shanshan Xiao ◽

Hang Yu ◽

Yunfei Xie ◽

Yahui Guo ◽

Jiajia Fan ◽

...

Keyword(s):

Essential Oil ◽

Cinnamomum Camphora ◽

Anti Inflammatory

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In vivo anti-inflammatory and antinociceptive activity of the crude extract and fractions from Rosa canina L. fruits

Journal of Ethnopharmacology ◽

10.1016/j.jep.2007.03.029 ◽

2007 ◽

Vol 112 (2) ◽

pp. 394-400 ◽

Cited By ~ 78

Author(s):

Didem Deliorman Orhan ◽

Ali Hartevioğlu ◽

Esra Küpeli ◽

Erdem Yesilada

Keyword(s):

Crude Extract ◽

Antinociceptive Activity ◽

Rosa Canina ◽

Anti Inflammatory

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In vivo Anti-Inflammatory and Antinociceptive Activity Evaluation of Phenolic Compounds from Sideritis stricta

Zeitschrift für Naturforschung C ◽

10.1515/znc-2007-7-810 ◽

2007 ◽

Vol 62 (7-8) ◽

pp. 519-525 ◽

Cited By ~ 24

Author(s):

Esra Küpeli ◽

Erdem Yeșilada ◽

İhsan Caliș ◽

Nurten Ezer ◽

Keyword(s):

Phenolic Compounds ◽

2D Nmr ◽

Inhibitory Effect ◽

Acetone Extract ◽

Antinociceptive Activity ◽

Flavonoid Glycosides ◽

Phenolic Fraction ◽

Phenolic Components ◽

Anti Inflammatory

An acetone extract obtained from aerial parts of S. stricta Boiss. & Heldr. apud Bentham, its fractions and phenolic compounds were investigated for their in vivo anti-inflammatory and antinociceptive activities. For the anti-inflammatory activity and for the antinociceptive activity assessment, carrageenan-induced hind paw edema and p-benzoquinone-induced abdominal constriction tests were used, respectively. The acetone extract of the plant and its phenolic fraction exhibited potent inhibitory activity against both bioassay models in mice. From the active phenolic fraction a well-known phenylethanoid glycoside, verbascoside (acteoside) (1), and two flavonoid glycosides, isoscutellarein 7-O-[6‴-O-acetyl-β-D-allopyranosyl-(1→2)]-β-D-glucopyranoside (2) and isoscutellarein 7-O-[6‴-O-acetyl-β-D-allopyranosyl-(1→2)]-6‴-O-acetyl-β-d-glucopyranoside (3), were isolated. During phytochemical studies we also isolated a methoxyflavone, xanthomicrol (4), from the non-polar fraction. The structures of the isolated compounds were established by spectroscopic evidence (UV, IR, 1D- and 2D-NMR, MS). Although antinociceptive and anti-inflammatory activities of the phenolic components were found not significant in the statistical analysis, compounds 1 to 3 showed a notable activity without inducing any apparent acute toxicity as well as gastric damage. Furthermore, a mixture of flavonoid glycosides (2 + 3) exhibited a significant inhibitory effect in both models at a higher dose

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Anti-inflammatory and Antinociceptive Activity of Ouabain in Mice

Mediators of Inflammation ◽

10.1155/2011/912925 ◽

2011 ◽

Vol 2011 ◽

pp. 1-11 ◽

Cited By ~ 35

Author(s):

Danielle Ingrid Bezerra de Vasconcelos ◽

Jacqueline Alves Leite ◽

Luciana Teles Carneiro ◽

Márcia Regina Piuvezam ◽

Maria Raquel Vitorino de Lima ◽

...

Keyword(s):

Immune Response ◽

Mast Cell ◽

Inflammatory Process ◽

Mast Cell Degranulation ◽

Regulatory Mechanisms ◽

Antinociceptive Activity ◽

Paw Edema ◽

Anti Inflammatory ◽

First Time

Ouabain, an inhibitor of the Na+/K+-ATPase pump, was identified as an endogenous substance of human plasma. Ouabain has been studied for its ability to interfere with various regulatory mechanisms. Despite the studies portraying the ability of ouabain to modulate the immune response, little is known about the effect of this substance on the inflammatory process. The aim of this work was to study the effects triggered by ouabain on inflammation and nociceptive models. Ouabain produced a reduction in the mouse paw edema induced by carrageenan, compound 48/80 and zymosan. This anti-inflammatory potential might be related to the inhibition of prostaglandin E2, bradykinin, and mast-cell degranulation but not to histamine. Ouabain also modulated the inflammation induced by concanavalin A by inhibiting cell migration. Besides that, ouabain presented antinociceptive activity. Taken these data together, this work demonstrated, for the first time, that ouabain presentedin vivoanalgesic and anti-inflammatory effects.

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Glycemic profile, inflammatory markers, biochemical and histopathological parameters of mice fed with fermented dairy beverage with clove essential oil

Research Society and Development ◽

10.33448/rsd-v10i4.13826 ◽

2021 ◽

Vol 10 (4) ◽

pp. e22110413826

Author(s):

Keicy Sandy Silvestre de Souza ◽

Anna Christina de Almeida ◽

Stephanie Pedrosa de Oliveira ◽

Igor Viana Brandi ◽

Sérgio Henrique Sousa Santos ◽

...

Keyword(s):

Essential Oil ◽

Biochemical Parameters ◽

Experimental Period ◽

Potassium Sorbate ◽

Histopathological Analysis ◽

Clove Essential Oil ◽

Anti Inflammatory ◽

Fermented Dairy

Previous studies have shown that the clove essential oil concentration of 200 mg/kg did not present toxicity and histopathological changes in the liver. Based on the evidence presented in this study, we decided to evaluate in vivo the ability of a previously developed fermented dairy beverage, tested only in vitro, in which the standard chemical preservative, potassium sorbate, was replaced by clove essential oil (2 μl/ml), to be a functional food. To this end, twenty-four male Swiss mice were divided into three groups and submitted insulin sensitivity (IST) and glucose tolerance (GTT) testing, evaluation of physiological and biochemical parameters, histopathological analysis and quantification of the expression of anti-inflammatory and pro-inflammatory cytokines in the liver, after administering daily of the fermented dairy beverage with or without clove essential oil by gavage during 30 days. The group that received the fermented dairy beverage with clove essential oil showed a lower glycemic level in GTT, a higher sensitivity to insulin and a higher glucose decay constant rate (Kitt), with relation to the other groups. In the evaluation of physiological parameters there were no signs of toxicity in the mice during the experimental period. The biochemical parameters, histopathological analysis and expression of anti-inflammatory (IL-10) and pro-inflammatory (IL1β, IL6 e TNFα) cytokines in the liver of the mice, was not significantly affected by the treatment. These results corroborated by bioinformatics analysis demonstrate that the fermented dairy beverage with clove essential oil it can function as a substitute for conventional chemical preservatives and reduce glycemic levels.

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Anti-Inflammatory and Physicochemical Characterization of the Croton rhamnifolioides Essential Oil Inclusion Complex in β-Cyclodextrin

Biology ◽

10.3390/biology9060114 ◽

2020 ◽

Vol 9 (6) ◽

pp. 114

Author(s):

Anita Oliveira Brito Pereira Bezerra Martins ◽

Almir Gonçalves Wanderley ◽

Isabel Sousa Alcântara ◽

Lindaiane Bezerra Rodrigues ◽

Francisco Rafael Alves Santana Cesário ◽

...

Keyword(s):

Essential Oil ◽

Vascular Permeability ◽

Inflammatory Diseases ◽

Physicochemical Characterization ◽

Major Constituent ◽

Paw Edema ◽

Popular Medicine ◽

Anti Inflammatory

Croton rhamnifolioides is used in popular medicine for the treatment of inflammatory diseases. The objective of this study was to characterize and evaluate the anti-inflammatory effect of C. rhamnifolioides essential oil complexed in β-cyclodextrin (COEFC). The physicochemical characterization of the complexes was performed using different physical methods. The anti-inflammatory activity was evaluated in vivo by ear edema, paw edema, cotton pellet-induced granuloma, and vascular permeability by Evans blue extravasation. The mechanism of action was validated by molecular docking of the major constituent into the cyclooxygenase-2 (COX-2 enzyme). All doses of the COEFC reduced acute paw edema induced by carrageenan and dextran, as well as vascular permeability. Our results suggest the lowest effective dose of all samples inhibited the response induced by histamine or arachidonic acid as well as the granuloma formation. The complexation process showed that the pharmacological effects were maintained, however, showing similar results using much lower doses. The results demonstrated an involvement of the inhibition of pathways dependent on eicosanoids and histamine. Complexation of β-cyclodextrin/Essential oil (β-CD/EO) may present an important tool in the study of new compounds for the development of anti-inflammatory drugs.

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