scholarly journals A Novel Pathway for Bacterial Membrane Vesicle Formation

Oleoscience ◽  
2018 ◽  
Vol 18 (5) ◽  
pp. 227-231
Author(s):  
Tatsuya YAMAMOTO ◽  
Nobuhiko NOMURA ◽  
Masanori TOYOFUKU
Keyword(s):  
Membrane Vesicle ◽  
Vesicle Formation ◽  
Bacterial Membrane

scholarly journals In Vitro Incorporation of GPI-Anchored Proteins Into Human Erythrocytes and Their Fate in the Membrane

Blood ◽  
1998 ◽  
Vol 91 (5) ◽  
pp. 1784-1792 ◽  
Author(s):  
Gianluca Civenni ◽  
Samuel T. Test ◽  
Urs Brodbeck ◽  
Peter Bütikofer
Keyword(s):  
Membrane Vesicle ◽  
Human Erythrocytes ◽  
Membrane Extraction ◽  
Membrane Microdomains ◽  
Membrane Insertion ◽  
Vesicle Formation ◽  
Calcium Loading ◽  
Lipid Moiety ◽  
Triton X

Abstract In many different cells, glycosylphosphatidylinositol (GPI)-anchored molecules are clustered in membrane microdomains that resist extraction by detergents at 4°C. In this report, we identified the presence of such domains in human erythrocytes and examined the ability of exogenously-added GPI-anchored molecules to colocalize with the endogenous GPI-anchored proteins in these detergent-insoluble complexes. We found that the addition to human erythrocytes of three purified GPI-anchored proteins having different GPI lipid moieties resulted in their efficient and correct incorporation into the membrane. The extent of membrane insertion was dependent on the intactness of the GPI lipid moiety. However, unlike the endogenous GPI-anchored proteins, the in vitro incorporated GPI molecules were not resistant to membrane extraction by Triton X-100 at 4°C. In addition, in contrast to the endogenous GPI-anchored proteins, they were not preferentially released from erythrocytes during vesiculation induced by calcium loading of the cells. These results suggest that in vitro incorporated GPI-linked molecules are excluded from pre-existing GPI-enriched membrane areas in human erythrocytes and that these microdomains may represent the sites of membrane vesicle formation.

Membrane vesicle formation is associated with pyocin production under denitrifying conditions inPseudomonas aeruginosa PAO1

2013 ◽  
Vol 16 (9) ◽  
pp. 2927-2938 ◽  
Author(s):  
Masanori Toyofuku ◽  
Shengmin Zhou ◽  
Isao Sawada ◽  
Naoki Takaya ◽  
Hiroo Uchiyama ◽  
...  
Keyword(s):  
Membrane Vesicle ◽  
Vesicle Formation

scholarly journals Reduced Production of Bacterial Membrane Vesicles Predicts Mortality in ST45/USA600 Methicillin-Resistant Staphylococcus aureus Bacteremia

Antibiotics ◽  
2019 ◽  
Vol 9 (1) ◽  
pp. 2 ◽  
Author(s):  
Somrita Dey ◽  
Smitha Gudipati ◽  
Christopher Giuliano ◽  
Marcus J. Zervos ◽  
Jonathan M. Monk ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Clinical Microbiology ◽  
Methicillin Resistant Staphylococcus Aureus ◽  
Membrane Vesicle ◽  
Membrane Vesicles ◽  
Bacterial Membrane ◽  
Staphylococcus Aureus Bacteremia ◽  
Immune Biomarkers ◽  
Microbial Biomarkers

Immune biomarkers can stratify mortality risk in staphylococcal bacteremia. Microbial biomarkers may provide more consistent signals during early infection. We demonstrate that in ST45/USA600 bacteremia, bacterial membrane vesicle production in vitro predicts clinical mortality (773 vs. 116 RFU, survivors vs. decedents, p < 0.0001). Using a threshold of 301 relative fluorescence units (RFU), the sensitivity and specificity of the membrane vesicles to predict mortality are 78% and 90%, respectively. This platform is facile, scalable and can be integrated into clinical microbiology lab workflows.

scholarly journals Interaction of quorum signals with outer membrane lipids: insights into prokaryotic membrane vesicle formation

2008 ◽  
Vol 69 (2) ◽  
pp. 491-502 ◽  
Author(s):  
Lauren Mashburn-Warren ◽  
Jörg Howe ◽  
Patrick Garidel ◽  
Walter Richter ◽  
Frank Steiniger ◽  
...  
Keyword(s):  
Outer Membrane ◽  
Membrane Lipids ◽  
Membrane Vesicle ◽  
Vesicle Formation

scholarly journals Extracellular Release of Antigenic Proteins byHelicobacter pylori

1998 ◽  
Vol 66 (6) ◽  
pp. 2984-2986 ◽  
Author(s):  
Ping Cao ◽  
Mark S. McClain ◽  
Mark H. Forsyth ◽  
Timothy L. Cover
Keyword(s):  
Helicobacter Pylori ◽  
Culture Supernatant ◽  
Genomic Library ◽  
Membrane Vesicle ◽  
Broth Culture ◽  
Vesicle Formation ◽  
Antigenic Proteins ◽  
Extracellular Release ◽  
Secretion Pathways ◽  
H Pylori

ABSTRACT Screening a Helicobacter pylori genomic library with antisera raised against H. pylori broth culture supernatant resulted in the identification of six antigens: urease, HspB, Lpp20, DnaK, MsrA, and a cysteine-rich 28-kDa protein (designated HcpA). H. pylori antigens may be released into the extracellular space by multiple mechanisms, including specific secretion pathways, autolysis, and membrane vesicle formation.

scholarly journals 2pB_SS4-1One particle physical property analysis of bacterial membrane vesicle using high-speed AFM

Microscopy ◽  
2018 ◽  
Vol 67 (suppl_2) ◽  
pp. i23-i23
Author(s):  
Yousuke Kikuchi ◽  
Nozomu Obana ◽  
Masanori Toyofuku ◽  
Noriyuki Kodera ◽  
Toshio Ando ◽  
...  
Keyword(s):  
High Speed ◽  
Membrane Vesicle ◽  
Physical Property ◽  
Bacterial Membrane ◽  
Property Analysis

scholarly journals Antibiotics Stimulate Formation of Vesicles inStaphylococcus aureusin both Phage-Dependent and -Independent Fashions and via Different Routes

2018 ◽  
Vol 63 (2) ◽  
pp. e01439-18 ◽  
Author(s):  
Federica Andreoni ◽  
Masanori Toyofuku ◽  
Carmen Menzi ◽  
Ratchara Kalawong ◽  
Srikanth Mairpady Shambat ◽  
...  
Keyword(s):  
Whole Blood ◽  
Ex Vivo ◽  
Membrane Vesicle ◽  
Membrane Vesicles ◽  
Vesicle Formation ◽  
Independent Manner ◽  
Content Type ◽  
Lactam Antibiotic ◽  
Underlying Mechanisms

ABSTRACTBacterial membrane vesicle research has so far focused mainly on Gram-negative bacteria. Only recently have Gram-positive bacteria been demonstrated to produce and release extracellular membrane vesicles (MVs) that contribute to bacterial virulence. Although treatment of bacteria with antibiotics is a well-established trigger of bacterial MV formation, the underlying mechanisms are poorly understood. In this study, we show that antibiotics can induce MVs through different routes in the important human pathogenStaphylococcus aureus. DNA-damaging agents and antibiotics inducing the SOS response triggered vesicle formation in lysogenic strains ofS. aureusbut not in their phage-devoid counterparts. The β-lactam antibiotics flucloxacillin and ceftaroline increased vesicle formation in a prophage-independent manner by weakening the peptidoglycan layer. We present evidence that the amount of DNA associated with MVs formed by phage lysis is greater than that for MVs formed by β-lactam antibiotic-induced blebbing. The purified MVs derived fromS. aureusprotected the bacteria from challenge with daptomycin, a membrane-targeting antibiotic, bothin vitroandex vivoin whole blood. In addition, the MVs protectedS. aureusfrom killing in whole blood, indicating that antibiotic-induced MVs function as a decoy and thereby contribute to the survival of the bacterium.

scholarly journals Molecular Dissection of Internalization of Porphyromonas gingivalis by Cells using Fluorescent Beads Coated with Bacterial Membrane Vesicle

2005 ◽  
Vol 30 (2) ◽  
pp. 81-91 ◽  
Author(s):  
Kayoko Tsuda ◽  
Atsuo Amano ◽  
Kyohei Umebayashi ◽  
Hiroaki Inaba ◽  
Ichiro Nakagawa ◽  
...  
Keyword(s):  
Porphyromonas Gingivalis ◽  
Membrane Vesicle ◽  
Bacterial Membrane ◽  
Molecular Dissection ◽  
Fluorescent Beads
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