scholarly journals Optimization of ultrasonic-assisted enzymatic hydrolysis conditions for the production of antioxidant hydrolysates from porcine liver by using response surface methodology

2017 ◽  
Vol 30 (11) ◽  
pp. 1612-1619 ◽  
Author(s):  
Hui-Chuan Yu ◽  
Fa-Jui Tan
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Response Surface ◽  
Porcine Liver ◽  
Ultrasonic Assisted ◽  
Hydrolysis Conditions

scholarly journals Antioxidant Hydrolysate Production From Bovine Collagen With Trypsin; Optimized by Response Surface Methodology

2021 ◽  
Author(s):  
Babak Pakbin ◽  
Samaneh Allahyari ◽  
Shaghayegh Pishkhan Dibazar ◽  
Wolfram Manuel Brück ◽  
Roghayeh Vahidi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Response Surface ◽  
Protein Hydrolysate ◽  
Meat Industry ◽  
Bovine Collagen ◽  
Dpph Method ◽  
Hydrolysis Conditions ◽  
Bovine Gelatin

Abstract Objective: Gelatin is a byproduct of meat industry and its hydrolysates showed several functionalities such as antioxidant activity. Response surface methodology (RSM) is a statistical method to mode and optimize biological processes. The purpose of this study was to describe and optimize the enzymatic hydrolysis conditions including time, temperature, pH, and enzyme/substrate ratio (E/S) to produce protein hydrolysate with antioxidant functionality from bovine gelatin by RSM. The scavenging activity was also evaluated using the DPPH method.Results: In this study, we developed and evaluated a model using RSM to describe and optimize conditions of enzymatic hydrolysis of bovine gelatin by trypsin to produce a protein hydrolysate with antioxidant activity. The model was observed and fitted with desirable adequacy and sufficiency. We found that the antioxidant activity increased significantly (P < 0.05) with increasing pH, E/S ratio and time of enzymatic process. However, temperature had no significant (P < 0.05) effect on the antioxidant activity of the hydrolysate. The optimum hydrolysis conditions were observed at the temperature 30-50 °C, pH 8.0, E/S ratio at 2.5 after 2 h of trypsin hydrolysis. The results showed that the hydrolysate under these conditions had greater antioxidant activity.

Optimization of Enzymatic Hydrolysis with Cryotin F on Antioxidative Activities for Shrimp Hydrolysate Using Response Surface Methodology

2009 ◽  
Vol 14 (4) ◽  
pp. 323-328 ◽  
Author(s):  
Yang-Bong Lee ◽  
Sivakumar Raghavan ◽  
Min-Hee Nam ◽  
Mi-Ae Choi ◽  
Navam S. Hettiarachchy ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Response Surface

scholarly journals Optimization of enzymatic hydrolysis of guar gum using response surface methodology

2012 ◽  
Vol 51 (8) ◽  
pp. 1600-1605 ◽  
Author(s):  
Deepak Mudgil ◽  
Sheweta Barak ◽  
B. S. Khatkar
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Response Surface ◽  
Guar Gum ◽  
Hydrolysis Of
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