Optimization of enzymatic hydrolysis conditions for the production of antioxidant peptides from muscles of Nemipterus japonicus and Exocoetus volitans using response surface methodology

Amino Acids ◽  
2011 ◽  
Vol 43 (1) ◽  
pp. 337-345 ◽  
Author(s):  
Shabeena Yousuf Naqash ◽  
R. A. Nazeer
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Response Surface ◽  
Antioxidant Peptides ◽  
Nemipterus Japonicus ◽  
Hydrolysis Conditions

scholarly journals Antioxidant Hydrolysate Production From Bovine Collagen With Trypsin; Optimized by Response Surface Methodology

2021 ◽  
Author(s):  
Babak Pakbin ◽  
Samaneh Allahyari ◽  
Shaghayegh Pishkhan Dibazar ◽  
Wolfram Manuel Brück ◽  
Roghayeh Vahidi ◽  
...  
Keyword(s):  
Antioxidant Activity ◽  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Response Surface ◽  
Protein Hydrolysate ◽  
Meat Industry ◽  
Bovine Collagen ◽  
Dpph Method ◽  
Hydrolysis Conditions ◽  
Bovine Gelatin

Abstract Objective: Gelatin is a byproduct of meat industry and its hydrolysates showed several functionalities such as antioxidant activity. Response surface methodology (RSM) is a statistical method to mode and optimize biological processes. The purpose of this study was to describe and optimize the enzymatic hydrolysis conditions including time, temperature, pH, and enzyme/substrate ratio (E/S) to produce protein hydrolysate with antioxidant functionality from bovine gelatin by RSM. The scavenging activity was also evaluated using the DPPH method.Results: In this study, we developed and evaluated a model using RSM to describe and optimize conditions of enzymatic hydrolysis of bovine gelatin by trypsin to produce a protein hydrolysate with antioxidant activity. The model was observed and fitted with desirable adequacy and sufficiency. We found that the antioxidant activity increased significantly (P < 0.05) with increasing pH, E/S ratio and time of enzymatic process. However, temperature had no significant (P < 0.05) effect on the antioxidant activity of the hydrolysate. The optimum hydrolysis conditions were observed at the temperature 30-50 °C, pH 8.0, E/S ratio at 2.5 after 2 h of trypsin hydrolysis. The results showed that the hydrolysate under these conditions had greater antioxidant activity.

Optimization of Enzymatic Hydrolysis with Cryotin F on Antioxidative Activities for Shrimp Hydrolysate Using Response Surface Methodology

2009 ◽  
Vol 14 (4) ◽  
pp. 323-328 ◽  
Author(s):  
Yang-Bong Lee ◽  
Sivakumar Raghavan ◽  
Min-Hee Nam ◽  
Mi-Ae Choi ◽  
Navam S. Hettiarachchy ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Response Surface

scholarly journals Optimization of enzymatic hydrolysis of guar gum using response surface methodology

2012 ◽  
Vol 51 (8) ◽  
pp. 1600-1605 ◽  
Author(s):  
Deepak Mudgil ◽  
Sheweta Barak ◽  
B. S. Khatkar
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Response Surface ◽  
Guar Gum ◽  
Hydrolysis Of

Study on Juice Yield by Compound Enzymolysis in Cloudy Apple Juice

2021 ◽  
Vol 1032 ◽  
pp. 45-50
Author(s):  
Tian Cai ◽  
Pu Shun Xi ◽  
Cheng Cheng Kou ◽  
Yue Yi Zhang ◽  
Xue Hui Cao ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Optimal Condition ◽  
Response Surface ◽  
Apple Juice ◽  
Juice Yield

The compound enzymolysis was studied to improve cloudy apple juice yield. Pectinase, cellulase and xylanase with high decomposition activity for cloudy apple juice were selected for compound enzymolysis. Response surface methodology was used to analyze the optimal additive amount. Results showed that, the effect of interaction between cellulase and xylanase on juice yield was extremely significant (P < 0.01). The optimal condition of complex enzymatic hydrolysis was: pectinase 0.16%, cellulase 0.57% and xylanase 0.12%. The better condition of compound enzymolysis was 45 °C for 60 min. The juice yield of cloudy apple juice significantly improved after optimized enzymolysis, which reached up to 83%.

scholarly journals Optimization of alkali pretreatment of wheat straw to be used as substrate for biofuels production  

2013 ◽  
Vol 59 (No. 12) ◽  
pp. 537-542 ◽  
Author(s):  
K. Jaisamut ◽  
L. Paulová ◽  
P. Patáková ◽  
M. Rychtera ◽  
K. Melzoch
Keyword(s):  
Response Surface Methodology ◽  
Enzymatic Hydrolysis ◽  
Sodium Hydroxide ◽  
Wheat Straw ◽  
Response Surface ◽  
Fermentable Sugars ◽  
Alkali Pretreatment ◽  
The Media ◽  
Pretreatment Conditions ◽  
Expert Designer

Alkali pretreatment of wheat straw was optimized by response surface methodology to maximize yields of fermentable sugars in subsequent enzymatic hydrolysis and to remove maximum lignin in order to improve rheological attributes of the media. The effects of pretreatment conditions on biomass properties were studied using the Expert Designer software. Concentration of sodium hydroxide and temperature were the factors most affecting pretreatment efficiency. At the optimum (80&deg;C, 39 min, 0.18 g NaOH and 0.06 g lime per g of raw biomass), 93.1 &plusmn; 1.0% conversion of cellulose to glucose after enzymatic hydrolysis and 80.3 &plusmn; 1.2% yield of monosaccharides (glucose plus xylose and arabinose) from cellulose and hemicellulose of wheat straw were achieved.

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