scholarly journals Ralstonia solanacearum: A Bacterial Disease and Its Biological Control by Essential Oils on Solanum tuberosum L.

2018 ◽  
Author(s):  
Cristina Ruiz Alvarado ◽  
Ramón Jaime Holguin Peña ◽  
Edgar Omar Rueda Puente
Keyword(s):  
Biological Control ◽  
Solanum Tuberosum ◽  
Essential Oils ◽  
Ralstonia Solanacearum ◽  
Solanum Tuberosum L ◽  
Bacterial Disease

scholarly journals Obtaining of transgenic potato (Solanum tuberosum L.) cultivar IPB CP3 containing LYZ‐C gene resistant to bacterial wilt disease

2021 ◽  
Vol 26 (1) ◽  
pp. 48
Author(s):  
Pasmawati Pasmawati ◽  
Aris Tjahjoleksono ◽  
Suharsono Suharsono
Keyword(s):  
Solanum Tuberosum ◽  
Bacterial Wilt ◽  
Solanum Tuberosum L ◽  
Transgenic Potato ◽  
Potato Production ◽  
Wilt Disease ◽  
Bacterial Disease ◽  
Gene Encoding ◽  
Bacterial Diseases ◽  
Bacterial Wilt Disease

Bacterial wilt caused by Ralstonia solanacearum is one of the most important bacterial diseases in potato production. This study aimed to obtain the transgenic potato (Solanum tuberosum L.) cultivar IPB CP3, containing LYZ‐C gene encoding for lysozyme type C, resistant to bacterial disease caused by R. solanacearum. Genetic transformation using Agrobacterium tumefaciens LBA4404 to 124 internode explants resulted in the transformation efficiency of about 47.58% with a regeneration efficiency of approximately 30.51%. Gene integration analysis showed that 16 clones were confirmed as transgenic clones containing the LYZ‐C gene. Analysis of resistance to R. solanacearum of three transgenic clones showed that all three transgenic clones were more resistant than a non‐transgenic one. This result showed that the LYZ‐C gene integrated in the genome of transgenic potato increased the resistance of potato plants to R. solanacearum. We obtained two transgenic clones considered resistant to bacterial wilt disease.

scholarly journals Razas, biovares y mecanismos de resistencia de aislados de Ralstonia solanacearum en el cultivo de papa (Solanum tuberosum L.) en Nicaragua

La Calera ◽  
2021 ◽  
Vol 21 (37) ◽  
Author(s):  
Jorge Alberto Fornos Blanco ◽  
Juan Carlos Rivera Mora ◽  
Isaías Ezequiel Sánchez Gómez ◽  
Arnulfo José Monzón Centeno ◽  
Danessa Joseph Ramírez Reynoza
Keyword(s):  
Solanum Tuberosum ◽  
Ralstonia Solanacearum ◽  
Solanum Tuberosum L

La papa (Solanum tuberosum L.) es el tercer cultivo alimenticio más importante del mundo en términos de consumo humano, después del arroz y del trigo, es un alimento básico para países desarrollados como Europa y Estados Unidos.  Este cultivo es afectado por problemas fitosanitarios, entre los que sobresale Ralstonia solanacearum. El objetivo de este estudio fue identificar mediante prueba fenotípica los mecanismos de resistencia de la bacteria Ralstonia solanacearum, aislada del cultivo de papa (S. tuberosum L.). La colecta de muestras se realizó en fincas de la reserva natural Miraflor y Tisey del departamento de Estelí. La bacteria fue aislada a partir de tejido vegetal mediante la prueba de flujo bacteriano y por técnica de dilución a partir de las muestras de suelo. La identificación de biovares se realizó con pruebas individuales de carbohidratos y la caracterización de razas fue mediante la prueba de hipersensibilidad Ralstonia solanacearum en hojas de tabaco. Se identificaron 19 aislados con características de Ralstonia solanacearum que manifestaron reacciones positivas a las pruebas de Oxidasa, Catalasa y KOH 3%, además se caracterizaron por su crecimiento acuoso, colonias grandes, blancas y centro de color rosado en el medio agar cloruro de tetrazolium (TZC 1%), con la prueba individual de azúcares se identificaron 14 aislados de R. solanacearum, como biovar 1, y los cinco restantes como biovar 3. Las reacciones en hojas de tabaco después de la infiltración de la bacteria mostraron que los 19 aislados de R. solanacearum pertenecen a la raza 1. Se identificaron 12 aislados de R. solanacearum con mecanismo de resistencia AmpC hiperproducido y siete aislados considerados como salvajes.

Evaluation of Biological Control Agents and Conventional Products for Post-Harvest Application on Potato (Solanum tuberosum L.) to Manage Leak

2020 ◽  
Vol 97 (5) ◽  
pp. 477-488
Author(s):  
Andrew K Hollingshead ◽  
Nora L. Olsen ◽  
Mike Thornton ◽  
Jeff Miller ◽  
David A. Schisler ◽  
...  
Keyword(s):  
Biological Control ◽  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Biological Control Agents ◽  
Post Harvest

scholarly journals Chemical Composition of Floral Essential Oils of Five Potato (Solanum tuberosum L.) Cultivars

2008 ◽  
Vol 51 (5) ◽  
pp. 177-178 ◽  
Author(s):  
Song-Mun Kim ◽  
Jeong-Mi Yu ◽  
Hak-Tae Lim ◽  
Hai-Ying Wang
Keyword(s):  
Solanum Tuberosum ◽  
Chemical Composition ◽  
Essential Oils ◽  
Solanum Tuberosum L

scholarly journals Evaluation of stored potato (Solanum tuberosum L.) for soft rot bacteria in Ibadan, Nigeria

2021 ◽  
Vol 2 (1) ◽  
pp. 53-60
Author(s):  
Durojaye Hammed Abiodun ◽  
Owoeye Tolulope Abisola
Keyword(s):  
Solanum Tuberosum ◽  
Pseudomonas Syringae ◽  
Ralstonia Solanacearum ◽  
Solanum Tuberosum L ◽  
Bacterial Pathogen ◽  
Soft Rot ◽  
Pectobacterium Carotovorum ◽  
Storage House ◽  
Serious Disease ◽  
And Storage

Potato (Solanum tuberosum L.) is a tuberous crop from the Sola-naceae family which is a source of starch and food to many in Nige-ria, however, its production is being hampered in field and storage by bacterial pathogen which causes rot of the stored tubers. To this end, the study aimed at screening potatoes from different stores within Ibadan for the evaluation and diversity of bacteria pathogens responsible for rot in potatoes. Damaged stored potato samples were collected from different locations in Ibadan for studies, these samples were isolated for bacterial pathogen and characterized bio-chemically and observed under the microscope for identification. Three genera of bacteria were observed to be responsible for potato rot in the study and they include Pectobacterium carotovorum, Pseu-domonas syringae and Ralstonia solanacearum. Of all the isolated bacteria, Pectobacterium carotovorum has the highest occurrence with a frequency of 60%, while Pseudomonas syringae has a fre-quency of 33% and Ralstonia solanacearum has a frequency of 27%. The pathogenicity of the isolates were tested and this revealed that Pectobacterium carotovorum is the most virulent with a severity score of 4.3, while Ralstonia solanacearum follows with a score of 3.3 and a score of 2.7 was recorded for Pseudomonas syringae, while the control had a score of 0. The diversity and differences shown in the isolated bacteria indicated that potato rot is a serious disease which is caused by different bacteria and need an integrated ap-proach for its control from the field of harvesting to the storage house.

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