scholarly journals The Red Microalga Galdieria as a Promising Organism for Applications in Biotechnology

2020 ◽  
Author(s):  
Mária Čížková ◽  
Milada Vítová ◽  
Vilém Zachleder
Keyword(s):  
Red Microalga

scholarly journals Transcriptomic survey reveals multiple adaptation mechanisms in response to nitrogen deprivation in marine Porphyridium cruentum

PLoS ONE ◽  
2021 ◽  
Vol 16 (11) ◽  
pp. e0259833
Author(s):  
Li Wei ◽  
Wuxin You ◽  
Zhengru Xu ◽  
Wenfei Zhang
Keyword(s):  
Lipid Metabolism ◽  
De Novo ◽  
Nitrate Assimilation ◽  
Porphyridium Cruentum ◽  
Metabolic Reprogramming ◽  
Nitrogen Stress ◽  
Nitrogen Deprivation ◽  
Marine Microalga ◽  
Nutrient Environment ◽  
Red Microalga

Single-cell red microalga Porphyridium cruentum is potentially considered to be the bioresource for biofuel and pharmaceutical production. Nitrogen is a kind of nutrient component for photosynthetic P. cruentum. Meanwhile, nitrogen stress could induce to accumulate some substances such as lipid and phycoerythrin and affect its growth and physiology. However, how marine microalga Porphyridium cruentum respond and adapt to nitrogen starvation remains elusive. Here, acclimation of the metabolic reprogramming to changes in the nutrient environment was studied by high-throughput mRNA sequencing in the unicellular red alga P. cruentum. Firstly, to reveal transcriptional regulation, de novo transcriptome was assembled and 8,244 unigenes were annotated based on different database. Secondly, under nitrogen deprivation, 2100 unigenes displayed differential expression (1134 upregulation and 966 downregulation, respectively) and some pathways including carbon/nitrogen metabolism, photosynthesis, and lipid metabolism would be reprogrammed in P. cruentum. The result demonstrated that nitrate assimilation (with related unigenes of 8–493 fold upregulation) would be strengthen and photosynthesis (with related unigenes of 6–35 fold downregulation) be impaired under nitrogen deprivation. Importantly, compared to other green algae, red microalga P. cruentum presented a different expression pattern of lipid metabolism in response to nitrogen stress. These observations will also provide novel insight for understanding adaption mechanisms and potential targets for metabolic engineering and synthetic biology in P. cruentum.

scholarly journals Thermostable phycocyanin from the red microalga Cyanidioschyzon merolae, a new natural blue food colorant

2016 ◽  
Vol 29 (3) ◽  
pp. 1233-1239 ◽  
Author(s):  
D. Y. Rahman ◽  
F. D. Sarian ◽  
A. van Wijk ◽  
M. Martinez-Garcia ◽  
M. J. E. C. van der Maarel
Keyword(s):  
Cyanidioschyzon Merolae ◽  
Food Colorant ◽  
Red Microalga

A highly branched storage polyglucan in the thermoacidophilic red microalga Galdieria maxima cells

2007 ◽  
Vol 43 (1) ◽  
pp. 78-83 ◽  
Author(s):  
I. N. Stadnichuk ◽  
L. R. Semenova ◽  
G. P. Smirnova ◽  
A. I. Usov
Keyword(s):  
Red Microalga

Influence of physiological factors on the lysis effect of Cytophaga on the red microalga Rhodella reticulata

2000 ◽  
Vol 88 (2) ◽  
pp. 358-363 ◽  
Author(s):  
T. Toncheva-Panova ◽  
J. Ivanova
Keyword(s):  
Physiological Factors ◽  
Rhodella Reticulata ◽  
Red Microalga

Inhibition of the growth of the red microalga porphyridium sp. by limitation of nutrient transfer

2007 ◽  
Vol 55 (3) ◽  
pp. 263-267 ◽  
Author(s):  
Avi Rotem ◽  
Jose C. Merchuk ◽  
Shoshana Malis Arad
Keyword(s):  
Nutrient Transfer ◽  
Red Microalga

scholarly journals Genes Involved in the Endoplasmic Reticulum N-Glycosylation Pathway of the Red Microalga Porphyridium sp.: A Bioinformatic Study

2014 ◽  
Vol 15 (2) ◽  
pp. 2305-2326 ◽  
Author(s):  
Oshrat Levy-Ontman ◽  
Merav Fisher ◽  
Yoram Shotland ◽  
Yacob Weinstein ◽  
Yoram Tekoah ◽  
...  
Keyword(s):  
Endoplasmic Reticulum ◽  
Glycosylation Pathway ◽  
Red Microalga

GLYCOPROTEIN MOIETY IN THE CELL WALL OF THE RED MICROALGA PORPHYRIDIUM SP. (RHODOPHYTA) AS THE BIORECOGNITION SITE FOR THE CRYPTHECODINIUM COHNII-LIKE DINOFLAGELLATE

1999 ◽  
Vol 35 (6) ◽  
pp. 1276-1281 ◽  
Author(s):  
Michal Ucko ◽  
Roshan Prakash Shrestha ◽  
Pnina Mesika ◽  
Dudy Bar-Zvi ◽  
Shoshana Malis Arad
Keyword(s):  
Cell Wall ◽  
Crypthecodinium Cohnii ◽  
Red Microalga

The effect of low temperature on fatty acid composition and tocopherols of the red microalga, Porphyridium cruentum

2007 ◽  
Vol 19 (3) ◽  
pp. 223-227 ◽  
Author(s):  
Yaşar Durmaz ◽  
Margarida Monteiro ◽  
Narcisa Bandarra ◽  
Şevket Gökpinar ◽  
Oya Işik
Keyword(s):  
Fatty Acid Composition ◽  
Fatty Acid ◽  
Low Temperature ◽  
Acid Composition ◽  
Porphyridium Cruentum ◽  
Red Microalga

scholarly journals Harvesting and Separation Technique of Porphyridium cruentum Polysaccharide Using Ultrafiltration Membrane

2016 ◽  
Vol 19 (2) ◽  
pp. 110
Author(s):  
Hasanah Hasanah ◽  
Iriani Setyaningsih ◽  
Uju Uju
Keyword(s):  
Membrane Permeability ◽  
Large Scale ◽  
Internal Resistance ◽  
Ultrafiltration Membrane ◽  
Porphyridium Cruentum ◽  
Membrane Characterization ◽  
Ultrafiltration Process ◽  
Medium Culture ◽  
Red Microalga

Red microalga Porphyridium cruentum secreting polysaccharides into its medium culture. Harvesting<br />and separation of polysaccharide of P. cruentum usually use centrifugation and in large scale needs high<br />cost. The use of ultrafiltration membrane can be one of the alternatives for harvesting and separation of<br />P. cruentum polysaccharide. This study aimed to determine the characteristic of membrane and permeat<br />from harvesting and separation of P. cruentum polysaccharide using ultrafiltration. Research consisted of<br />four stages : membrane characterization, cultivation of P. cruentum, harvesting using 0.05 μm ultrafiltration<br />membrane, and polysaccharide separation using 0.01 μm ultrafiltration membrane. Characterization of<br />membrane permeability and internal resistance on ultrafiltration 0.05 μm dan 0.01 μm were 137.32 L/m2hbar<br />and 62.38 L/m2hbar and 0.01 barm2h/L and 0.02 barm2h/L, respectively. Harvesting using ultrafiltration 0.05<br />μm produced flux 131.37-94.75 L/m2h, biomass rejection 96% and permeate with OD (Optical Density) (0.01<br />± 0.00), viscosity (2.4 ± 0.17 cp), pH (8 ± 0.00), and salinity (42.37 ± 0.11 ‰). Separation of polysaccharide<br />using ultrafiltration 0.05 μm produced flux 58.11-51.53 L/m2h and permeate with viscosity (2.2 ± 0.30 cp),<br />pH (7.8 ± 0.01), and salinity (38.73 ± 0.05 ‰). Ultrafiltration process decreased OD, viscosity, and salinity<br />of permeate.

scholarly journals Biomass and phycocyanin content of heterotrophic Galdieria sulphuraria 074G under maltodextrin and granular starches–feeding conditions

2019 ◽  
Vol 32 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Delicia Yunita Rahman ◽  
Fean Davisunjaya Sarian ◽  
Marc J. E. C. van der Maarel
Keyword(s):  
Carbon Sources ◽  
Natural Food ◽  
Galdieria Sulphuraria ◽  
Microalgal Cultivation ◽  
Food Colorant ◽  
Major Disadvantage ◽  
Enzyme Cocktail ◽  
Cheap Alternative ◽  
Biomass Yields ◽  
Red Microalga

Abstract A major disadvantage of microalgal cultivation is limited biomass yields due to the autotrophic lifestyle of most microalgal species. Heterotrophic growth on a suitable carbon source and oxygen can overcome such limitations. The red microalga Galdieria sulphuraria strain 074G grows heterotrophically on glucose and a number of other carbon sources while constitutively producing photopigments, including the blue-colored phycocyanin, a natural food colorant. Galdieria sulphuraria strain 074G grew well on maltodextrins as well as on granular starch in combination with the enzyme cocktail Stargen002. The maltodextrin cultures produced 2 mg phycocyanin per gram substrate, being slightly more than on glucose. The phycocyanin extracted from maltodextrin-grown cultures was thermostable up to 55 °C. Maltodextrins can be a cheap alternative to glucose syrups for the production of phycocyanin as natural food colorant.

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