Influence of the total concentration and the profile of volatile fatty acids on polyhydroxyalkanoates (PHA) production by mixed microbial cultures

Polyhydroxyalkanoates (PHAs) production from lignocellulosic biomass using mixed microbial cultures (MMC) is a potential cheap alternative for reducing the use of petroleum-based plastics. In this study, an MMC adapted to acidogenic effluent from dark fermentation (DF) of exhausted sugar beet cossettes (ESBC) has been tested in order to determine its capability to produce PHAs from nine different synthetic mixtures of volatile fatty acids (VFAs). The tests consisted of mixtures of acetic, propionic, butyric, and valeric acids in the range of 1.5–9.0 g/L of total acidity and with three different valeric:butyric ratios (10:1, 1:1, and 1:10). Experimental results have shown a consistent preference of the MMC for the butyric and valeric acids as carbon source instead other shorter acids (propionic or acetic) in terms of PHA production yield (estimated in dry cell weight basis), with a maximum value of 23% w/w. Additionally, valeric-rich mixtures have demonstrated to carry out a fast degradation process but with poor final PHA production compared with high butyric mixtures. Finally, high initial butyric and valeric concentrations (1.1 g/L and 4.1 g/L) have demonstrated to be counterproductive to PHA production.

A novel coconut-malt extract medium increases growth rate of morels in pure culture

Morels are gourmet wild edible mushrooms that can grow on several substrates with significant growth rate variations. Such variations have hindered the development of a standardized culture media to promote morel’s sustainable production. The aim of this study is developing a novel culture media that takes advantage of coconut water as a complementary component of culture media. Coconut water has been extensively used as a growth-promoting component for plant tissue cultures; however, its application as component of fungi cultivation medium has not been fully developed. This study confirms that coconut water can be efficiently used as culture media component for morels using a kinetic characterization. Morchella sp. kinetic growth is evaluated in different cultures: agar, malt extract agar (MEA), lactose, coconut water (15%) and combinations of them. Kinetic growth parameters (lag phase, λ and maximum specific growth rate, µmax) are estimated using primary modeling methods. Among the selected models, the best fit is achieved using Baranyi’s model. A significant increase from 15.8% to 43.4% of the µmax values was observed when culture media (agar, lactose, MEA) is supplemented with coconut water. The largest values of µmax are obtained in MEA-coconut cultures (21.13 ± 0.43–22.57 ± 0.35). Micro-sclerotia and late sclerotia are observed in all cultures containing coconut water justifying the development of a feasible and cost-effective way of culturing morels. The results demonstrate that coconut water can be used for formulation of standard media for morel cultivation leading to a cheap alternative to produce dense mycelium and promote sclerotia formation.

Large Eddy Simulation with wall functions of Ahmed body

Large Eddy Simulation with Wall Function (WFLES) is known to be a cheap alternative to classical LES methods for simulation of flow where large and complex computational meshes are typically required. This makes it attractive for engineering applications. However experience of applying such methods to complex turbulent flows with flow separation and reattachment is still little-known in literature. In this work WFLES of flow around simplified car body with slant angle equal to 25 degrees and ReL = 2.8 · 106 is carried out on Octree mesh to demonstrate the capabilities and limitations of the method in such type of the flow. The results on a series of meshes show that even though the general flow topology is well captured, the critical part of the flow on the slant is hardly predicted even on 100 mln mesh. It is concluded that the prediction of separation above the slant requires significant mesh refinement even in the frame of WFLES.

Typhoon: A vortex-lattice code for assessing dynamic stability characteristics of hydrofoil crafts

In this paper an open-source implementation of the vortex-lattice method to perform a dynamic stability analysis for hydrofoil crafts is discussed. The difference with existing vortex-lattice codes is the addition of a free-surface boundary condition which is needed to analyse surface piercing foils. This code, called Typhoon, can be used to perform a dynamic stability analysis (DSA) on hydrofoil vessels. The goal of this code is to have an easy-to-use and cheap alternative to compare different designs in early design stages. This paper gives a brief background to all the concepts used, followed by a short theoretical explanation of the vortex-lattice method. The second part of this paper focuses on a practical example of how this code can be used on an example.

Moisture-Induced Pattern of Gases and Physicochemical Indices in Corn Straw and Cow Manure Composting

This study investigated the altering effect of moisture on the emission pattern of gases and the evolutionary dynamics of physicochemical indices in corn straw and cow manure composting. Exploring this effect was reasonable to unravel the use of moisture as a cheap alternative to control gaseous emissions and improve the final properties of compost. The nutrient dynamics of the compost showed 21.6% losses in total organic carbon content, with a 33.3% increase in total nitrogen content at the end of composting. All the gases (CH4, CO2, N2O and NH3) yielded a common emission pattern despite the differences in moisture content. Except for CH4, the peak and stable emission periods of all the gases were observed on the 5th day (thermophilic phase) and after the 27th day (late mesophilic phase) of composting, respectively. Emission reductions of 89%, 91%, 95% and 100% were recorded for CH4, CO2, N2O and NH3, respectively, during the late mesophilic phase of composting. From the study, the 65% moisture content was efficient in reducing the loss rate of the gasses and nutrient contents of the compost. This study would enable farmers to channel organic residues generated into compost while minimizing pollution and nutrient losses associated with the composting process.

Accumulation pattern of polycyclic aromatic hydrocarbons using Plantago lanceolata L. as passive biomonitor

Biomonitors are considered a cheap alternative of active air samplers, especially where spatial pattern of air quality is to be monitored, requiring numerous parallel measurements. Of higher plants, Plantago lanceolata L. has been proven a good monitor species with proper accumulation capacity. While biomonitoring studies are difficult to compare due to inherent errors such as the diverse plant material used in different studies, the No. 227 OECD GUIDELINE FOR THE TESTING OF CHEMICALS: Terrestrial Plant Test: Vegetative Vigour Test provides a tool to test extract of aerosol samples under controlled laboratory conditions. In our study, this guideline was followed to experimentally treat Plantago with the aqueous extract of a diesel exhaust sample. Accumulation pattern of polyaromatic hydrocarbons (PAHs) was assessed and compared to samples collected in the field. Unlike most studies reported in the literature, both in the experimentally treated and field Plantago samples, high ratio of high molecular weight PAHs was experienced. Distribution pattern of accumulated PAHs showed strong correlation between the experimentally treated sample and most of the field plantain samples, underlying the usefulness of laboratory treatments for bioaccumulation studies.

Remediation of Oily Waste using Soil Organic Nutrient Stimulant

Aims: This present study aim at assessing the efficacy of soil-organic nutrient stimulant in the remediation of oily waste. Study design: Preparation of Soil-goat dung mix was used as stimulant for the remediation of oily waste. Place and duration of study: The study was carried out at the Department of Microbiology University of Uyo, Uyo, Akwa Ibom state, Nigeria in the dry season months of January - March Methodology: Remediation of oily waste using soil - organic (goat dung) nutrient stimulant was assessed for 12 weeks using standard culture-dependent microbiological, chemical and enzyme activity assay procedures. Results: The results indicate increased counts of hydrocarbon utilizing bacteria, fungi and actinomycetes with remediation time. Microorganisms belonging to the genera Bacillus, Pseudomonas, Acinetobacter, Alcaligenes, Serratia, Penicillium, Aspergillus, Cladosporium, Rhodococcus, Nocardia and Streptomyces were recovered from the remediated waste. The pH of the soil-goat dung treatment ranged from 6.5 ± 0.02 to 7.1 ± 0.05. Enzyme activity by dehydrogenase and urease were higher than phenol oxidase with time. PAHs were below detectable limits (< 0.01) and reduction in total petroleum hydrocarbon was 99.3% for the remediated waste. Conclusion: Overall, enhanced microbial activities correlated positively with reduction in Total Petroleum Hydrocarbon and PAH composition which resulted in ecofriendly waste product. Soil-goat dung stimulant can therefore serve as a cheap alternative in the management of oily waste.

Recent advances and perspectives in efforts to reduce the production and application cost of microbial flocculants

Microbial flocculants are macromolecular substances produced by microorganisms. Due to its non-toxic, harmless, and biodegradable advantages, microbial flocculants have been widely used in various industrial fields, such as wastewater treatment, microalgae harvest, activated sludge dewatering, heavy metal ion adsorption, and nanoparticle synthesis, especially in the post-treatment process of fermentation with high safety requirement. However, compared with the traditional inorganic flocculants and organic polymeric flocculants, the high production cost is the main bottleneck that restricts the large-scale production and application of microbial flocculants. To reduce the production cost of microbial flocculant, a series of efforts have been carried out and some exciting research progresses have been achieved. This paper summarized the research advances in the last decade, including the screening of high-yield strains and the construction of genetically engineered strains, search of cheap alternative medium, the extraction and preservation methods, microbial flocculants production as an incidental product of other biological processes, combined use of traditional flocculant and microbial flocculant, and the production of microbial flocculant promoted by inducer. Moreover, this paper prospects the future research directions to further reduce the production cost of microbial flocculants, thereby promoting the industrial production and large-scale application of microbial flocculants.

MO429A NOVEL METHOD LINESCAN-DRIVEN FOR SNGFR MEASUREMENTS AS ALTERNATIVE TO HIGH FULL FRAME MULTIPHOTON MICROSCOPY ACQUISITION

Background and Aims Renal micropuncture, which requires the direct access to the renal tubules, has been for long time the technique of choice to measure the single nephron glomerular filtration rate (SNGFR) in animal models, but this approach is challenging by virtue of complex animal preparation and numerous careful steps. The introduction of intravital multiphoton microscopy (MPM) permitted to improve the study of renal functions exploiting the high laser penetration and the optical sectioning capacity. Previous MPM studies measuring in vivo the SNGFR relied on fast full frame acquisition during the filtration process obtainable with microscope resonant scanners, which represent optional expensive equipment able to reach very high acquisition speed. In this work we propose an innovative linescan-based MPM method to calculate SNGFR in rodents doable without using the fast acquisition rate offered by resonant scanners. Method An in vivo MPM approach was used to measure the SNGFR in control Munich Wistar Frömter rats (MWF) and to test the feasibility of the innovative linescan approach. In order to validate this method in conditions known for reduced and increased SNGFR, it was applied to ischemia reperfusion injury (IRI) and low-dose dopamine treated conditions, respectively. Results The glomeruli connected to S1 proximal tubules extending at least 100 μm from the exit of the Bowman’s space were chosen for the measurement. A linescan path starting from the urinary pole and crossing many times the tubular lumen orthogonally to the cellular wall was hand drawn. The linescan was acquired soon after a i.v. bolus of low-molecular weight fluorescent marker was injected. The tubular length, the mean diameter and the transit time of the fluorescent marker within two lines of interest (called cross1 and cross2) were measured to obtain the SNGFR. SNGFR measured in control rats was comparable with previous reported data both at MPM and micropuncture. Significantly higher values compared to control were obtained in 3 μg/kg/min dopamine-treated rats. In IRI-treated rats the SNGFR was reduced about 35% compared to the controls. Conclusion The results achieved with our linescan method were quite similar to those obtained with conventional micropuncture, suggesting that the two methods overlap for the normal, dopamine and IRI treatment. Our results show that linescan approach is a promising and cheap alternative to the fast full frame acquisition for the investigation of SNGFR in health and disease, offering results comparable to conventional micropuncture with unprecedent temporal resolution.

Alloplastic or Autologous? Bone Chips versus PEEK Cage for Lumbar Interbody Fusion in Degenerative Spondylolisthesis

Introduction This study was conducted to compare bone-filled intervertebral cages with autologous bone chips for instrumented lumbar interbody fusion in patients with spinal stenosis and degenerative spondylolisthesis. Methods Surgery consisted of posterior instrumentation and decompression, diskectomy, and intervertebral fusion using a polyetheretherketone (PEEK) cage surrounded and filled with spongious bone chips (group 1, n = 57) or spongious bone chips alone (group 2, n = 37). The choice of method was left to the discretion of the surgeon. Postoperative results were prospectively evaluated using a standardized protocol. Radiological assessment included fusion rates and vertebral height, while clinical assessment included the visual analog scale (VAS) and Oswestry Disability Index (ODI). Results In group 1, a mean of 1.38 ± 0.64 segments were fused. In group 2, a mean of 1.58 ± 0.65 segments were fused. In both groups, the VAS for back pain and leg pain and the ODI improved without significant differences between the two groups. Osseous fusion was documented by computerized tomography in 73% in group 1 and 89% in group 2 after a mean of 18 months. The loss of height was 2.8 ± 4.0% in group 1 and 2.4 ± 5.2% in group 2. Conclusion Regardless of whether a PEEK cage filled with spongious bone chips or spongious bone chips alone were used for lumbar interbody fusion, clinical parameters improved significantly after surgery. There were no significant differences in the rate of bony fusion and loss of height between the two groups. The results of this nonrandomized cohort study indicate that the implantation of autologous spongious bone chips harvested during the decompression procedure is a useful and cheap alternative to an intervertebral cage in patients with degenerative pseudospondylolisthesis.