scholarly journals Biomass and phycocyanin content of heterotrophic Galdieria sulphuraria 074G under maltodextrin and granular starches–feeding conditions

2019 ◽  
Vol 32 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Delicia Yunita Rahman ◽  
Fean Davisunjaya Sarian ◽  
Marc J. E. C. van der Maarel
Keyword(s):  
Carbon Sources ◽  
Natural Food ◽  
Galdieria Sulphuraria ◽  
Microalgal Cultivation ◽  
Food Colorant ◽  
Major Disadvantage ◽  
Enzyme Cocktail ◽  
Cheap Alternative ◽  
Biomass Yields ◽  
Red Microalga

Abstract A major disadvantage of microalgal cultivation is limited biomass yields due to the autotrophic lifestyle of most microalgal species. Heterotrophic growth on a suitable carbon source and oxygen can overcome such limitations. The red microalga Galdieria sulphuraria strain 074G grows heterotrophically on glucose and a number of other carbon sources while constitutively producing photopigments, including the blue-colored phycocyanin, a natural food colorant. Galdieria sulphuraria strain 074G grew well on maltodextrins as well as on granular starch in combination with the enzyme cocktail Stargen002. The maltodextrin cultures produced 2 mg phycocyanin per gram substrate, being slightly more than on glucose. The phycocyanin extracted from maltodextrin-grown cultures was thermostable up to 55 °C. Maltodextrins can be a cheap alternative to glucose syrups for the production of phycocyanin as natural food colorant.

scholarly journals Thermostable phycocyanin from the red microalga Cyanidioschyzon merolae, a new natural blue food colorant

2016 ◽  
Vol 29 (3) ◽  
pp. 1233-1239 ◽  
Author(s):  
D. Y. Rahman ◽  
F. D. Sarian ◽  
A. van Wijk ◽  
M. Martinez-Garcia ◽  
M. J. E. C. van der Maarel
Keyword(s):  
Cyanidioschyzon Merolae ◽  
Food Colorant ◽  
Red Microalga

scholarly journals Natural food colorant from blackcurrant spray‐dried powder obtained by enzymatic treatment: Characterization and acceptability

2020 ◽  
Author(s):  
Hernando Toscano Martínez ◽  
Maite Gagneten ◽  
Paulo Díaz‐Calderón ◽  
Javier Enrione ◽  
Daniela Salvatori ◽  
...  
Keyword(s):  
Enzymatic Treatment ◽  
Natural Food ◽  
Food Colorant ◽  
Spray Dried

scholarly journals Crocetin Isolated from the Natural Food Colorant Saffron Reduces Intracellular Fat in 3T3-L1 Adipocytes

Foods ◽  
2020 ◽  
Vol 9 (11) ◽  
pp. 1648
Author(s):  
Elena Jiménez-Ortega ◽  
Aitana Braza-Boïls ◽  
Miguel Burgos ◽  
Natalia Moratalla-López ◽  
Manuel Vicente ◽  
...  
Keyword(s):  
Lipid Accumulation ◽  
Low Cost ◽  
Natural Food ◽  
Peroxisome Proliferator ◽  
Synthetic Dyes ◽  
Potential Candidate ◽  
Peroxisome Proliferator Activated Receptor ◽  
Food Colorant ◽  
Enhancer Binding Protein ◽  
Diphenyltetrazolium Bromide

Saffron, as a food colorant, has been displaced by low-cost synthetic dyes. These have unhealthy properties; thus, their replacement with natural food colorants is an emerging trend. Obesity is a worldwide health problem due to its associated comorbidities. Crocetin esters (crocins) are responsible for the red saffron color. Crocetin (CCT) exhibits healthful properties. We aimed to broaden the existing knowledge on the health properties of CCT isolated from saffron, to facilitate its consideration as a healthy natural food colorant in the future. We evaluated the ability of CCT (1 and 5 μM) to reduce lipid accumulation during the differentiation of 3T3-L1 preadipocytes. Intracellular fat was quantified by Oil Red O staining. CTT cytotoxicity was measured using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The number and size of lipid droplets were analyzed using WimLipid software. The expression of adipogenic genes (CCAAT/enhancer-binding protein (C/EBPβ, C/EBPδ, C/EBPα), and peroxisome proliferator-activated receptor γ (PPARγ)) was analyzed using quantitative real-time PCR (qRT-PCR). CCT 5 μM decreased intracellular fat by 22.6%, without affecting viability or lipid droplet generation, via a decrease in C/EBPα expression, implicated in lipid accumulation. Thus, CCT is a potential candidate to be included in dietary therapies aimed at reversing adipose tissue accumulation in obesity.

A 90-day oral toxicity study of beta-carotene derived from Blakeslea trispora, a natural food colorant, in F344 rats

2005 ◽  
Vol 43 (7) ◽  
pp. 1127-1133 ◽  
Author(s):  
K. Nabae ◽  
T. Ichihara ◽  
A. Hagiwara ◽  
T. Hirota ◽  
Y. Toda ◽  
...  
Keyword(s):  
Beta Carotene ◽  
Toxicity Study ◽  
Blakeslea Trispora ◽  
Natural Food ◽  
Oral Toxicity ◽  
Food Colorant ◽  
F344 Rats

Colour evaluation of freeze-dried roselle extract as a natural food colorant in a model system of a drink

LWT ◽  
2008 ◽  
Vol 41 (8) ◽  
pp. 1437-1445 ◽  
Author(s):  
Kiattisak Duangmal ◽  
Busararat Saicheua ◽  
Suchitra Sueeprasan
Keyword(s):  
Model System ◽  
Natural Food ◽  
Food Colorant ◽  
Freeze Dried

scholarly journals The biosynthetic pathway for a thousand-year-old natural food colorant and citrinin in Penicillium marneffei

2014 ◽  
Vol 4 (1) ◽  
Author(s):  
Patrick C. Y. Woo ◽  
Ching-Wan Lam ◽  
Emily W. T. Tam ◽  
Kim-Chung Lee ◽  
Karrie K. Y. Yung ◽  
...  
Keyword(s):  
Biosynthetic Pathway ◽  
Penicillium Marneffei ◽  
Natural Food ◽  
Food Colorant

Tulip petal as a novel natural food colorant source: Extraction optimization and stability studies

2016 ◽  
Vol 91 ◽  
pp. 215-222 ◽  
Author(s):  
Muhammet Arici ◽  
Salih Karasu ◽  
Mehmet Baslar ◽  
Omer Said Toker ◽  
Osman Sagdic ◽  
...  
Keyword(s):  
Natural Food ◽  
Stability Studies ◽  
Food Colorant ◽  
Extraction Optimization ◽  
Source Extraction

scholarly journals Transport and Utilization of Hexoses and Pentoses in the Halotolerant Yeast Debaryomyces hansenii

1999 ◽  
Vol 65 (8) ◽  
pp. 3594-3598 ◽  
Author(s):  
Alexandra Nobre ◽  
Cândida Lucas ◽  
Cecília Leão
Keyword(s):  
Growth Parameters ◽  
Carbon Sources ◽  
Debaryomyces Hansenii ◽  
Transport Systems ◽  
Facilitated Diffusion ◽  
Xylose Transport ◽  
Halotolerant Yeast ◽  
Consumption Rates ◽  
Biomass Yields ◽  
Proton Uptake

ABSTRACT Debaryomyces hansenii is a yeast species that is known for its halotolerance. This organism has seldom been mentioned as a pentose consumer. In the present work, a strain of this species was investigated with respect to the utilization of pentoses and hexoses in mixtures and as single carbon sources. Growth parameters were calculated for batch aerobic cultures containing pentoses, hexoses, and mixtures of both types of sugars. Growth on pentoses was slower than growth on hexoses, but the values obtained for biomass yields were very similar with the two types of sugars. Furthermore, when mixtures of two sugars were used, a preference for one carbon source did not inhibit consumption of the other. Glucose and xylose were transported by cells grown on glucose via a specific low-affinity facilitated diffusion system. Cells derepressed by growth on xylose had two distinct high-affinity transport systems for glucose and xylose. The sensitivity of labeled glucose and xylose transport to dissipation of the transmembrane proton gradient by the protonophore carbonyl cyanidem-chlorophenylhydrazone allowed us to consider these transport systems as proton symports, although the cells displayed sugar-associated proton uptake exclusively in the presence of NaCl or KCl. When the V max values of transport systems for glucose and xylose were compared with glucose- and xylose-specific consumption rates during growth on either sugar, it appeared that transport did not limit the growth rate.

scholarly journals The Chemical and Cytotoxic Properties of Sambucus nigra Extracts—A Natural Food Colorant

2021 ◽  
Vol 13 (22) ◽  
pp. 12702
Author(s):  
Mariusz Banach ◽  
Barbara Khaidakov ◽  
Daria Korewo ◽  
Magdalena Węsierska ◽  
Wojciech Cyplik ◽  
...  
Keyword(s):  
Column Chromatography ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Membrane Separation ◽  
Natural Food ◽  
Peripheral Blood Mononuclear ◽  
Lung Cancers ◽  
Food Colorant ◽  
Blood Mononuclear Cells

Elderberry fruits contain valuable components that are beneficial to human health. Owing to the high content of anthocyanins, elderberry extracts can be used as natural food colorants with health-promoting properties. Moreover, the development of new natural food dyes enables the reduction in the use of synthetic ones. Anthocyanins-rich elderberry dry extracts (EDE) were prepared from the same batch of frozen fruits applying water extraction, followed by membrane separation (batch B1) or purification by column chromatography (batch B2) and then spray-dried. Subsequently, the content of anthocyanins, flavonols, and polyphenols was determined. The extract obtained with the application of column chromatography (B2) contained 33% anthocyanins, which is more than typical market standards, whereas the extract B1 contained 14% anthocyanins. The color properties of both extracts were also determined. Since water was used as an extractant, the extracts are well soluble in water and can therefore be used as a natural food colorant. The cytotoxic activity of both extracts was additionally determined using the MTT test and the tumor cells of the A-549, A-2780, MCF-7, Caco-2 line, and Peripheral blood mononuclear cells. It was revealed that both EDEs inhibit the proliferation of cancer cells, except those of the lung cancers. Extract B2 showed a much stronger cytotoxic effect. Additionally, both extracts stimulate the proliferation of peripheral blood mononuclear cells since they may have immunostimulatory properties.

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