scholarly journals Ambient Biobanking Solutions for Whole Blood Sampling, Transportation, and Extraction

2020 ◽  
Author(s):  
Armaity Nasarabadi Fouts ◽  
Alejandro Romero ◽  
James Nelson ◽  
Mike Hogan ◽  
Shanavaz Nasarabadi
Keyword(s):  
Whole Blood ◽  
Blood Sampling

Time Dependence of Whole Blood Aggregation in Response to Platelet Activating Factor (PAF)

1988 ◽  
Vol 59 (02) ◽  
pp. 162-163 ◽  
Author(s):  
R R Taylor ◽  
J Strophair ◽  
M Sturm ◽  
R Vandongen ◽  
L J Beilin
Keyword(s):  
Time Dependence ◽  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Platelet Activating Factor ◽  
Sampling Time ◽  
Blood Sampling ◽  
Impedance Aggregometry

SummaryThe aggregation/adhesion response to platelet activating factor (PAF) was studied in diluted whole blood by impedance aggregometry. The extent of aggregation varied directly with the interval between blood sampling and aggregation measurement over the first 30 minutes from sampling, then remained stable for the next 60 minutes of observation. This is an effect opposite to that described for aggregation to PAF in platelet rich plasma which, however, cannot be studied soon after sampling. Time dependence of aggregation is important and comparative measurements should be made during the period of stable aggregability.

scholarly journals An Anti-Nucleocapsid Antigen Sars-Cov-2 Total Antibody Assay Finds Comparable Results in Edta-Anticoagulated Whole Blood Obtained from Capillary and Venous Blood Sampling

Data ◽  
2020 ◽  
Vol 5 (4) ◽  
pp. 105
Author(s):  
Martin Risch ◽  
Marc Kovac ◽  
Corina Risch ◽  
Dorothea Hillmann ◽  
Michael Ritzler ◽  
...  
Keyword(s):  
Whole Blood ◽  
Venous Blood ◽  
Capillary Blood ◽  
Blood Sampling ◽  
Attractive Alternative ◽  
Antibody Assay ◽  
Antibody Assays ◽  
Antibody Titers ◽  
Antibody Levels ◽  
Venous Blood Sampling

Although SARS-CoV-2 antibody assays have been found to provide valid results in EDTA-anticoagulated whole blood, so far, they have not demonstrated that antibody levels in whole blood originating from capillary blood samples are comparable to antibody levels measured in blood from a venous origin. Here, blood is drawn simultaneously by capillary and venous blood sampling. Antibody titers are determined by an assay employing electrochemiluminescence (ECLIA) and SARS-CoV-2 total immunoglobulins are detected with specificity directed against the nucleocapsid antigen. Six individuals with confirmed COVID-19 and six individuals without COVID-19 are analyzed. Antibody titers in capillary venous whole blood did not show significant differences, and when corrected for hematocrit, they did not differ from the results obtained from serum. In conclusion, capillary sampled EDTA-anticoagulated whole blood seems to be an attractive alternative matrix for the evaluation of SARS-CoV-2 antibodies when employing ECLIA for detecting total antibodies directed against nucleocapsid antibodies.

A method for large volume blood sampling and transfusion in rats

1986 ◽  
Vol 250 (3) ◽  
pp. E331-E337
Author(s):  
H. R. Berthoud ◽  
W. B. Laughton ◽  
T. L. Powley
Keyword(s):  
Large Volume ◽  
Whole Blood ◽  
Superior Vena ◽  
Vena Cava ◽  
Blood Sampling ◽  
Indwelling Catheters ◽  
Multiple Channel ◽  
Glucose Levels ◽  
Include Blood Pressure ◽  
Volume Blood

A new protocol that makes it feasible to withdraw large volumes of whole blood from an individual rat within 1 h or less is described. This method involves the use of indwelling catheters for withdrawal of blood from the inferior vena cava with concurrent isovolemic replacement of whole blood into the superior vena cava. Simultaneity of the transfusion and withdrawal, strict equality of volumes, and a smooth exchange of blood are assured by the use of separate channels of the same multiple-channel peristaltic pump for withdrawal and replacement. Validation experiments using both anesthetized and unanesthetized rats indicate that several responses remain essentially undisturbed during large volume blood sampling; these parameters include blood pressure, heart rate, hematocrit, plasma hormones including insulin and glucagon, plasma glucose levels, and feeding behavior. Considerations of technical and physiological limitations of the protocol, including choice of catheters and catheter placement, pump, sampling parameters, and obtaining donor blood, are discussed.

A femoral arteriovenous shunt facilitates arterial whole blood sampling in animals

2002 ◽  
Vol 29 (3) ◽  
pp. 319-323 ◽  
Author(s):  
Bruno Weber ◽  
Cyrill Burger ◽  
Peter Biro ◽  
Alfred Buck
Keyword(s):  
Whole Blood ◽  
Blood Sampling ◽  
Arteriovenous Shunt

scholarly journals Detection of the Antibodies against Porcine Cytomegalovirus from Whole Blood Collected on the Blood Sampling Paper.

1994 ◽  
Vol 56 (1) ◽  
pp. 189-190 ◽  
Author(s):  
Tomoko TAJIMA ◽  
Takeshi HIRONAO ◽  
Taketsugu KAJIKAWA ◽  
Yoshihisa SUZUKI ◽  
Hitoshi KAWAMURA
Keyword(s):  
Whole Blood ◽  
Blood Sampling ◽  
Porcine Cytomegalovirus

Levels of Platelet Factor 3 in Citrate Plasma and in Whole Blood as Determined by A Chromogenic Peptide Substrate Assay

1979 ◽  
Author(s):  
H. Sandberg ◽  
A.-K. Gellerbring ◽  
L.-O. Andersson
Keyword(s):  
Whole Blood ◽  
Platelet Rich Plasma ◽  
Blood Sampling ◽  
Chromogenic Substrate ◽  
Plasma Samples ◽  
Peptide Substrate ◽  
Platelet Factor ◽  
No Release ◽  
Sensitive Method

A newly developed sensitive method for determination of platelet factor 3 (PF 3) using a chromogenic substrate (S 2238) (Sandberg and Andersson, Thromb. Res., in press) was used for comparison of the PF 3 levels in platelet-rich plasma (PRP) in citrate with the levels in PRP in the anticoagulant EDTA/citrate/PCE 1/theophylline. It was shown that in citrate PRP, release of PF 3 was started after about 20 minutes from blood sampling. Release of β-thromboglobulin (β-tg) was found to occur simultaneously with the release of PF 3. No release of PF 3 or β-tg could be detected within 3 hours from blood sampling in PRP in the EDTA/citrate/PCE 1/theophylline anticoagulant.The PF 3 level in whole blood was measured by a method which was a modification of the method used for plasma samples. It was found that, using the same donor, the PF 3 level of plasma and blood in the EDTA/citrate/PCE 1/theophylline anticoagulant was essentially the same. The levels of PF 3 found in blood upon standing in citrate or in EDTA/citrate/PCE 1/theophy11ine anticoagulant were in accordance with the levels found in plasma. Experiments with whole blood without any anticoagulant showed that release of PF 3 begin to occur simultaneously with release of β-tg, about 5 minutes before clotting. Thus the data show that PF 3 and βtg are released in parallel.

Capillary microsampling of whole blood for mouse PK studies: an easy route to serial blood sampling

Bioanalysis ◽  
2015 ◽  
Vol 7 (4) ◽  
pp. 449-461 ◽  
Author(s):  
Walter Korfmacher ◽  
Maria Fitzgerald ◽  
Yongyi Luo ◽  
Stacy Ho ◽  
Jie Wang ◽  
...  
Keyword(s):  
Whole Blood ◽  
Blood Sampling ◽  
Serial Blood ◽  
Serial Blood Sampling

Validation of a Non-Invasive Hemoglobin Estimation in Whole Blood Donors.

Blood ◽  
2012 ◽  
Vol 120 (21) ◽  
pp. 2280-2280
Author(s):  
Murtadha K. Al-Khabori ◽  
Khalil Al Farsi ◽  
Mohammed Al-Huneini ◽  
Shahina Daar ◽  
Abdulhakeem Al-Hashim ◽  
...  
Keyword(s):  
Correlation Coefficient ◽  
Whole Blood ◽  
Blood Donors ◽  
Conflicts Of Interest ◽  
Pearson Correlation ◽  
Tertiary Care ◽  
Blood Sampling ◽  
Normal Blood ◽  
Coefficient Of Determination ◽  
Non Invasive

Abstract Abstract 2280 Introduction: Pre-donation screening of blood donors are currently based on venous or capillary blood sampling. Adoption of a non-invasive hemoglobin estimation may increase blood donor recruitment. Masimo Pronto-7 Pulse CO-oximetry device is a spectrophotometry based device used to estimate the hemoglobin (Hb) level non-invasively, waiving the need of blood sampling. It has not been validated in normal blood donors. The primary objective of our study was to validate the pulse CO-oximetry based hemoglobin estimation in normal blood donors. Methods: We conducted a prospective observational study on 106 whole blood donors attending the blood bank of a tertiary care center over 4 weeks. We estimated a Spot Hemoglobin (Sp Hb) concentration using Masimo Pronto-7 Pulse CO-oximetry device (two measurements per donor) and compared it to a venous sample Hb (Reference Hemoglobin; Ref Hb) measured using Abbott CELL-DYN Sapphire hematology analyzer. We calculated Pearson correlation coefficient and coefficient of determination (R2). The multivariable linear regression model of predicting the estimation differences included age, gender, weight, height, blood pressure and reference hemoglobin. Results: We enrolled 106 donors (98 males, 8 females) with a mean age of 27 years (SD 6.2; 18–49) and a mean Ref Hb of 14.2 g/dL (SD 1.2; 11.5–17). The mean Sp Hb was 14.4 g/dL (SD 1.2; 11.3–16.7). The correlation coefficient between the Sp Hb and Ref Hb was 0.46 (R2 = 21%) with a mean difference of 0.2 g/dL (SD 1.2; −4.5 to 3). In the multivariable model, height (p =0.015) and Ref Hb level (p <0.001) were statistically significant predictors of the difference in measurement. There was a strong correlation between the two CO-oximetry Hb measurements (correlation coefficient 0.78, R2 = 60%). Conclusions: Our study demonstrated the validity of the CO-oximetry Hb measurement in normal blood donors and with good reproducibility. Larger prospective studies are needed to confirm our findings. Disclosures: No relevant conflicts of interest to declare.

scholarly journals Reliable quantification of 18F-GE-180 PET neuroinflammation studies using an individually scaled population-based input function or late tissue-to-blood ratio

2020 ◽  
Vol 47 (12) ◽  
pp. 2887-2900 ◽  
Author(s):  
Ralph Buchert ◽  
Meike Dirks ◽  
Christian Schütze ◽  
Florian Wilke ◽  
Martin Mamach ◽  
...  
Keyword(s):  
Blood Sample ◽  
Whole Blood ◽  
Input Function ◽  
Population Based ◽  
Blood Sampling ◽  
Tracer Activity ◽  
Arterial Blood Sampling ◽  
Arterial Blood ◽  
The Individual ◽  
Individual Input

Abstract Purpose Tracer kinetic modeling of tissue time activity curves and the individual input function based on arterial blood sampling and metabolite correction is the gold standard for quantitative characterization of microglia activation by PET with the translocator protein (TSPO) ligand 18F-GE-180. This study tested simplified methods for quantification of 18F-GE-180 PET. Methods Dynamic 18F-GE-180 PET with arterial blood sampling and metabolite correction was performed in five healthy volunteers and 20 liver-transplanted patients. Population-based input function templates were generated by averaging individual input functions normalized to the total area under the input function using a leave-one-out approach. Individual population-based input functions were obtained by scaling the input function template with the individual parent activity concentration of 18F-GE-180 in arterial plasma in a blood sample drawn at 27.5 min or by the individual administered tracer activity, respectively. The total 18F-GE-180 distribution volume (VT) was estimated in 12 regions-of-interest (ROIs) by the invasive Logan plot using the measured or the population-based input functions. Late ROI-to-whole-blood and ROI-to-cerebellum ratio were also computed. Results Correlation with the reference VT (with individually measured input function) was very high for VT with the population-based input function scaled with the blood sample and for the ROI-to-whole-blood ratio (Pearson correlation coefficient = 0.989 ± 0.006 and 0.970 ± 0.005). The correlation was only moderate for VT with the population-based input function scaled with tracer activity dose and for the ROI-to-cerebellum ratio (0.653 ± 0.074 and 0.384 ± 0.177). Reference VT, population-based VT with scaling by the blood sample, and ROI-to-whole-blood ratio were sensitive to the TSPO gene polymorphism. Population-based VT with scaling to the administered tracer activity and the ROI-to-cerebellum ratio failed to detect a polymorphism effect. Conclusion These results support the use of a population-based input function scaled with a single blood sample or the ROI-to-whole-blood ratio at a late time point for simplified quantitative analysis of 18F-GE-180 PET.

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