scholarly journals Microbial Diversity of Traditionally Processed Cheese from Northeastern Region of Transylvania (Romania)

2021 ◽  
Author(s):  
Éva György ◽  
Éva Laslo
Keyword(s):  
Foodborne Pathogens ◽  
Stenotrophomonas Maltophilia ◽  
Microscopic Fungi ◽  
Culture Methods ◽  
Selective Media ◽  
Enterococcus Durans ◽  
Northeastern Region ◽  
Staphylococcus Equorum ◽  
Identification And Characterization

The composition and production technology of the cheese are extremely diverse. There are a wide variety of microbial species on their surface, with a much smaller number inside of the product. The microbiota of the cheese may be composed of beneficial microorganisms, spoilage and foodborne pathogens. Identification and characterization of the microorganisms present in these products are important nutrition, food safety and technological aspects. During our work we evaluated the prevalence of allochthonous bacteria and microscopic fungi in traditionally processed cheeses from northeastern region of Transylvania, with classical microbiological culture methods. Based on the results the microbiota of the analysed cheeses was highly diversified. The identified bacteria with the highest prevalence from different selective media, were as follows: Escherichia coli, Enterococcus durans, Enterococcus faecalis, Shigella flexnerii, Proteus vulgaris, Stenotrophomonas maltophilia, Staphylococcus equorum subsp. equorum, Staphylococcus equorum subsp. linens, Halomonas alkaliphila, Kocuria rhizophila, Hafnia paralvei, Bacillus licheniformis and Klebsiella michiganensis.

Isolation, Identification, and Characterization of Foodborne Pathogens Isolated from Egg Internal Contents in China

2016 ◽  
Vol 79 (12) ◽  
pp. 2107-2112 ◽  
Author(s):  
ZHAO GE ◽  
SONG XUE ◽  
ZHAO JIANMEI ◽  
LI YUEHUA ◽  
WANG JUAN ◽  
...  
Keyword(s):  
Foodborne Pathogens ◽  
World Economy ◽  
Egg Production ◽  
Egg Laying ◽  
Cross Contamination ◽  
E Coli ◽  
Local Risk ◽  
Identification And Characterization ◽  
Foodborne Infections

ABSTRACT Eggs continue to be significant in terms of the world economy and human nutrition. The aim of this study was to estimate the prevalence of foodborne pathogens in a cross section of egg production types in China and to examine the virulence features of the isolated pathogens. Three hundred eggs from three provinces were tested for Salmonella, Escherichia coli, Staphylococcus aureus, and Campylobacter. Two eggs were positive for Salmonella, one was positive for both E. coli and S. aureus, and none were positive for Campylobacter. When comparing different egg laying systems, there were no significant differences in findings between packaged and unpackaged eggs and between battery cage and cage-free eggs, but there were significant differences in the findings among pathogens and among provinces. Other potential foodborne pathogens were identified in addition to the targeted pathogens. The virulence of Serratia marcescens was higher than that of the isolated Salmonella Typhimurium, and the virulence of Staphylococcus saprophyticus was comparable to that of the isolated S. aureus. These findings are important for local risk assessments concerning possible human foodborne infections via cross-contamination of eggs.

scholarly journals Separation, Identification, and Characterization of Microorganisms by Capillary Electrophoresis

2003 ◽  
Vol 67 (1) ◽  
pp. 38-51 ◽  
Author(s):  
Meera J. Desai ◽  
Daniel W. Armstrong
Keyword(s):  
Capillary Electrophoresis ◽  
Consumer Products ◽  
Small Sample ◽  
Instrumental Method ◽  
Additive Concentration ◽  
Culture Methods ◽  
Practical Applications ◽  
Minimal Sample ◽  
Identification And Characterization

SUMMARY The use of capillary electrophoresis (CE) for the analysis, identification, and characterization of microorganisms has been gaining in popularity. The advantages of CE, such as small sample requirements, minimal sample preparation, rapid and simultaneous analysis, ease of quantitation and identification, and viability assessment, make it an attractive technique for the analysis of microbial analytes. As this instrumental method has evolved, higher peak efficiencies have been achieved by optimizing CE conditions, such as pH, ionic strength, and polymer additive concentration. Experimental improvements have allowed better quantitation and more accurate results. Many practical applications of this technique have been investigated. Viability and identification of microbes can be accomplished in a single analysis. This is useful for evaluation of microbial analytes in consumer products. Diagnosis of microbe-based diseases is now possible, in some cases, without the need for culture methods. Microbe-molecule, virus-antibody, or bacteria-antibiotic interactions can be monitored using CE, allowing for the screening of possible drug candidates. Fermentation can be monitored using this system. This instrumental approach can be adapted to many different applications, including assessing the viability of sperm cells. Progress has been made in the development of microelectrophoresis instrumentation. These advances will eventually allow the development of small, dedicated devices for the rapid, repetitive analyses of specific microbial samples. Although these methods may never fully replace traditional approaches, they are proving to be a valuable addition to the collection of techniques used to analyze, quantitate, and characterize microbes. This review outlines the recent developments in this rapidly growing field.

scholarly journals GeneSippr: A Rapid Whole-Genome Approach for the Identification and Characterization of Foodborne Pathogens such as Priority Shiga Toxigenic Escherichia coli

PLoS ONE ◽  
2015 ◽  
Vol 10 (4) ◽  
pp. e0122928 ◽  
Author(s):  
Dominic Lambert ◽  
Catherine D. Carrillo ◽  
Adam G. Koziol ◽  
Paul Manninger ◽  
Burton W. Blais
Keyword(s):  
Escherichia Coli ◽  
Foodborne Pathogens ◽  
Whole Genome ◽  
Identification And Characterization

Identification and characterization of peptidases secreted by quahog parasite unknown (QPX), the protistan parasite of hard clams

2016 ◽  
Vol 122 (1) ◽  
pp. 21-33 ◽  
Author(s):  
E Rubin ◽  
GT Werneburg ◽  
E Pales Espinosa ◽  
DG Thanassi ◽  
B Allam
Keyword(s):  
Quahog Parasite Unknown ◽  
Hard Clams ◽  
Identification And Characterization

Identification and characterization of Vibrio harveyi associated with diseased abalone Haliotis diversicolor

2013 ◽  
Vol 103 (2) ◽  
pp. 133-139 ◽  
Author(s):  
Q Jiang ◽  
L Shi ◽  
C Ke ◽  
W You ◽  
J Zhao
Keyword(s):  
Vibrio Harveyi ◽  
Haliotis Diversicolor ◽  
Identification And Characterization

IDENTIFICATION AND CHARACTERIZATION OF 1,2-BN CYCLOHEXENE USING MICROWAVE SPECTROSCOPY

2016 ◽  
Author(s):  
Stephen Kukolich ◽  
Shih-Yuan Liu ◽  
Jacob Ishibashi ◽  
Adam Daly ◽  
Ming Sun
Keyword(s):  
Microwave Spectroscopy ◽  
Identification And Characterization
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