scholarly journals Investigation of Antimicrobial Activity of Medicinal Plant Extracts on Bacillus cereus Isolated from Soil

2021 ◽  
Vol In Press (In Press) ◽  
Author(s):  
Bahman Fazeli-Nasab ◽  
Laleh Shahraki-Mojahed ◽  
Mohammad Amir Hassanzadeh ◽  
Fatemeh Bidarnamani
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Growth Inhibition ◽  
Bacillus Cereus ◽  
Plant Extracts ◽  
Diffusion Method ◽  
Thymus Vulgaris ◽  
Taxus Baccata ◽  
Medicinal Plant Extracts ◽  
Effect On Growth

Background: Because many plant extracts have a significant inhibitory effect on pathogenic microorganisms, so we aimed to evaluate the antioxidant and antimicrobial activity of some plant extracts on Bacillus cereus isolated from soil. Methods: The chicory (Cichorium intybus L.), hyacinth (Hypericum perforatum L.), lavender (Lavandula angustifolia), yew (Taxus baccata), and thyme (Thymus vulgaris L.) plants were collected and identified in the botanical laboratory of the University of Zabol. To prepare the ethanolic extract, 40 g of dried leaves of plants were used in 400 cc of ethanol. Different strains of B. cereus used in this study were isolated from soil and identified by biochemical, bacteriological, and growth tests as well as standard tests. Antimicrobial effects were investigated by diffusion method in Müller Hinton agar medium using 6 mm paper discs according to Bauer and Kirby instructions as well as microdilution. Statistix ver10 software was used for statistical calculations. Mean comparisons were performed using the LSD at the 1% level, and Excel was also used to draw the figures. Results: The diameter of the growth inhibition zone of plant extracts against B. cereus at a dilution of 100 ppm was investigated, and it was found that different extracts had different effects on inhibiting the growth of B. cereus (P < 0.01). LSD test showed that thyme (T. vulgaris L.) had the highest (15 mm) effect on growth inhibition of strains 2 and 3 of B. cereus and the lowest (1 mm) effect on growth inhibition of strain 1 of B. cereus. The lowest MIC and MBC of thyme (T. vulgaris L.) against B. cereus samples were 3.1 and 6.2 ppm, respectively. Conclusions: Considering the side effects of chemical drugs and antibiotics, as well as the potential effect of medicinal plant extracts, especially T. vulgaris L. on B. cereus, it is recommended that T. vulgaris L. may inhibit the growth of B. cereus.

scholarly journals Screening research of 90% aqueous ethanolic plant extracts antimicrobial activity against Propionobacterium acnes — an etiological factor of acne occurrence

2020 ◽  
Vol 24 (1) ◽  
pp. 75-79
Author(s):  
G.V. Rusko
Keyword(s):  
Antimicrobial Activity ◽  
Side Effects ◽  
Plant Extracts ◽  
Antimicrobial Properties ◽  
Raw Material ◽  
Diffusion Method ◽  
Medicinal And Aromatic Plants ◽  
Evernia Prunastri ◽  
Phytochemical Study ◽  
Medicinal Plant Extracts

Annotation. Prolonged antibacterial therapy of acne, mainly by antibiotics of MLS-group (macrolides, lincosamides and streptogramin B), has a number of side effects, the main of which is the emergence of resistant strains. Considering the risks and side effects of traditional etiotropic acne therapy, the use of herbal medicines may be an alternative of this disease treatment. The purpose of this study was to perform a screening study of 90% aqueous-ethanolic medicinal plant extracts antimicrobial activity against P acnes test strains. Antimicrobial activity of 242 aqueous-ethanolic extracts of 183 medicinal and aromatic plants of various organs against two P. acnes strains: sensitive and MLS resistant by HB (Heart Brain) agar diffusion method was studied. Statistical processing of results has been caried by UTHSCSA ImageTool 2.0, Microsoft Office Excel 2011. Eupatorium cannabinum L. and Grindelia squarrosa (Pursh) Dun. aerial parts, Populus nigra L. fruits and needles, Helichrysum arenarium L., Evernia prunastri (L.) Ach. herbs, Calendula officinalis L. flowers, Geranium pratense L. rhizomes and Larix decidua Mill. needles exhibited strong antimicrobial activity (diameter of growth inhibition≥ 15 mm) against test strains. Representatives with antimicrobial properties against acne agents have been found among medicinal plants of Ukraine's flora. The detection of pronounced antimicrobial properties in various pharmacopoeial and non-pharmacopoeial plants necessitates the need for phytochemical study of the raw material to isolate and identify its active components.

Phenolic characterization and antimicrobial activity of folk medicinal plant extracts for their applications in olive production

2014 ◽  
Vol 35 (11) ◽  
pp. 1709-1718 ◽  
Author(s):  
Joana Boiteux ◽  
Carolina Soto Vargas ◽  
Pablo Pizzuolo ◽  
Gabriela Lucero ◽  
María Fernanda Silva
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plant ◽  
Plant Extracts ◽  
Medicinal Plant Extracts

scholarly journals In Vitro Antibacterial Activities of Various Ethanolic Medicinal Plant Extracts Against Some Human Pathogenic Bacteria

2020 ◽  
Vol 8 (6) ◽  
pp. 1272-1276
Author(s):  
Samir K. Ali ◽  
Ghorbat S. Ali ◽  
Berivan Abdulrahman Abdullah
Keyword(s):  
Medicinal Plant ◽  
Plant Extracts ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Nigella Sativa ◽  
Antibacterial Activities ◽  
Diffusion Method ◽  
Bacterial Isolates ◽  
Foeniculum Vulgare ◽  
Medicinal Plant Extracts

The widespread use of antibiotics often causes increase in the bacterial drugs resistance and causes many side effects in humans. Medical plants have antimicrobial effects against most pathogenic bacteria and can serve as harmless replacement to antibiotics. The aim of this study was to assess the antibacterial effect of five medicinal plant prevailed in Kurdistan region namely; Cinnamon (Cinnamomum cassia (L.) D.Don), Nigella (Nigella sativa L.), Allium (Allium hirtifolium Boiss.), Carrot (Daucus carota L.), and Fennel (Foeniculum vulgare Mill.) against pathogenic Gram positive bacteria (Staphylococcus aureus), and pathogenic Gram negative bacteria (Salmonella typhi and Escherichia coli). The evaluation of antibacterial activity for these plant extracts was carried out using agar-well diffusion method. Results showed that minimum inhibitory concentration (MIC) of A. hirtifolium against tested bacterial isolates were (25 mg/mL), the lowest MIC values for S. aureus were (25 mg/mL) observed with C. cassia, N. sativa and F. vulgare ethanolic extracts and the lowest MIC of D. carota against bacterial isolates were (50 mg/mL), Also, it was observed that S. aureus was more sensitive than S. typhi and E. coli to plant extracts. The ethanol plant extracts had potential antibacterial activities. However, further studies are required to identify the active compounds which could be used for the preparation of new antimicrobial agents and control the bacterial infectious diseases.

scholarly journals Towards Advances in Medicinal Plant Antimicrobial Activity: A Review Study on Challenges and Future Perspectives

2021 ◽  
Vol 9 (10) ◽  
pp. 2041
Author(s):  
Natalia Vaou ◽  
Elisavet Stavropoulou ◽  
Chrysa Voidarou ◽  
Christina Tsigalou ◽  
Eugenia Bezirtzoglou
Keyword(s):  
Antimicrobial Activity ◽  
Medicinal Plants ◽  
Medicinal Plant ◽  
Plant Extracts ◽  
Pathogenic Bacteria ◽  
Antimicrobial Agents ◽  
Mechanisms Of Action ◽  
Future Perspectives ◽  
Pharmacodynamic Profile ◽  
Medicinal Plant Extracts

The increasing incidence of drug- resistant pathogens raises an urgent need to identify and isolate new bioactive compounds from medicinal plants using standardized modern analytical procedures. Medicinal plant-derived compounds could provide novel straightforward approaches against pathogenic bacteria. This review explores the antimicrobial activity of plant-derived components, their possible mechanisms of action, as well as their chemical potential. The focus is put on the current challenges and future perspectives surrounding medicinal plants antimicrobial activity. There are some inherent challenges regarding medicinal plant extracts and their antimicrobial efficacy. Appropriate and optimized extraction methodology plant species dependent leads to upgraded and selective extracted compounds. Antimicrobial susceptibility tests for the determination of the antimicrobial activity of plant extracts may show variations in obtained results. Moreover, there are several difficulties and problems that need to be overcome for the development of new antimicrobials from plant extracts, while efforts have been made to enhance the antimicrobial activity of chemical compounds. Research on the mechanisms of action, interplay with other substances, and the pharmacokinetic and/or pharmacodynamic profile of the medicinal plant extracts should be given high priority to characterize them as potential antimicrobial agents.

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