Comparison of Antimicrobial Activity of Fluoride Varnishes Against Streptococcusmutans and Lactobacillus acidophilus: An In Vitro Study

Background: Topical fluoride products such as varnishes prevent dental caries by limiting demineralization and antibacterial properties. The structural and metabolic characteristics of cariogenic microorganisms are different from each other. Also, the formulation properties, concentration, and release behavior of fluoride vary in different varnishes. Objectives: In this study, we investigated the antibacterial effects of three types of common fluoride varnishes against two cariogenic bacteria of Streptococcus mutans (S.M.) and Lactobacillus acidophilus (L.A.). Methods: In this in vitro study, antibacterial effects of MI varnish [containing calcium phosphopeptide-amorphous calcium phosphate (CPP-ACP)], Polimo (containing xylitol), and FluoroDose varnishes were evaluated using disc diffusion method via measuring the diameter of the inhibition zone. The antibiotics of erythromycin and ampicillin were used as the positive control of the test. Analysis of variance (ANOVA) and post hoc tests were applied to compare the mean of non-growth zone diameter in the studied groups. Data were analyzed by SPSS 16. The statistical significance level was determined as P-value < 0.05. Results: The antibacterial effect of each varnish was optimal against both bacteria. In comparison, the mean diameter of the inhibition zone in MI varnish was significantly (P-value=0.019) higher than Polimo and FluoroDose brands on S.M.; however, this difference was not statistically significant for L.A. Furthermore, Polimo and FluoroDose varnishes showed similar antimicrobial effects against these bacteria. Conclusions: The use of these varnishes seems to be suitable for preventing tooth decay. MI varnish is preferable because of its higher antibacterial properties.

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Antiadherent and antibacterial properties of stainless steel and NiTi orthodontic wires coated with silver against Lactobacillus acidophilus—an in vitro study

Progress in Orthodontics ◽

10.1186/s40510-015-0110-0 ◽

2015 ◽

Vol 16 (1) ◽

Cited By ~ 15

Author(s):

Arun Rameshwar Mhaske ◽

Pradeep Chandra Shetty ◽

N. Sham Bhat ◽

C. S. Ramachandra ◽

S. M. Laxmikanth ◽

...

Keyword(s):

Stainless Steel ◽

Lactobacillus Acidophilus ◽

Antibacterial Properties ◽

In Vitro Study ◽

Vitro Study ◽

Orthodontic Wires

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Effectiveness of Metabolite Substance Filtrates of Actinomycetes isolates from Kebun Raya Bogor against the growth of Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi: In Vitro study

JURNAL BIOLOGI TROPIS ◽

10.29303/jbt.v21i1.2466 ◽

2021 ◽

Vol 21 (1) ◽

pp. 281

Author(s):

Muhammad Zaidan Tsani Ariandi ◽

Meiskha Bahar ◽

Hany Yusmaini ◽

Fajriati Zulfa ◽

Cut Fauziah ◽

...

Keyword(s):

Escherichia Coli ◽

Pseudomonas Aeruginosa ◽

In Vitro Study ◽

Inhibition Zone ◽

Salmonella Typhi ◽

Diffusion Method ◽

P Value ◽

E Coli ◽

Botanical Gardens

Actinomycetes are found in soils with loose, humus, dry characteristics and around plant roots. Actinomycetes produce secondary metabolite compounds as antibacterial. Escherichia coli, Pseudomonas aeruginosa and Salmonella typhi are Gram negative bacteria that can cause infection in humans. This study aims to determine the ability of the Actinomycetes metabolite filtrate from Bogor Botanical Gardens as an antibacterial agent against the growth of E. coli, P.aeruginosa and S. typhi in vitro. This study used an experimental design with samples of Actinomycetes isolats originating from the Bogor Botanical Gardens soil using the disc diffusion method on Muller Hinton Agar (MHA) media by looking at the clear zone of bacterial growth around the disc paper. Of the three concentration groups, namely 50%, 60%, and 70%, the largest average inhibition zone is found at a concentration of 70% with the average for the three test bacteria E. coli, P.aeruginosa and S. typhi respectively: 4.23 mm; 3.0 mm and 8.43 mm. The results of the Kruskal - Wallis test with p value = 0.01 showed that there was an effect of the Actinomycetes metabolite filtrate on the growth of the three tested bacteria as antibacterials.

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