scholarly journals Molecular Characterization of Carbapenem-Resistant Klebsiella pneumoniae Species Isolated From a Tertiary Hospital, Ankara, Turkey

2017 ◽  
Vol 10 (10) ◽  
Author(s):  
Nevreste Celikbilek ◽  
Ozlem Unaldi ◽  
Fisun Kirca ◽  
Aysegul Gozalan ◽  
Ziya C Acikgoz ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Molecular Characterization ◽  
Tertiary Hospital ◽  
Carbapenem Resistant

scholarly journals Molecular Characterization of Carbapenem-resistant, Colistin-resistant Klebsiella pneumoniae Isolates from a Tertiary Hospital in Jeonbuk, Korea

2021 ◽  
Vol 51 (3) ◽  
pp. 120-127
Author(s):  
Tae Hee Lee ◽  
Minhyeon Cho ◽  
Jaehyeon Lee ◽  
Joo-Hee Hwang ◽  
Chang-Seop Lee ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Molecular Characterization ◽  
Tertiary Hospital ◽  
Carbapenem Resistant

scholarly journals Molecular characterization of Carbapenem resistant Escherichia coli recovered from a tertiary hospital in Lebanon

PLoS ONE ◽  
2018 ◽  
Vol 13 (9) ◽  
pp. e0203323 ◽  
Author(s):  
Christel Dagher ◽  
Tamara Salloum ◽  
Sahar Alousi ◽  
Harout Arabaghian ◽  
George F. Araj ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Molecular Characterization ◽  
Tertiary Hospital ◽  
Carbapenem Resistant

scholarly journals Characterization of carbapenem‐resistant Klebsiella pneumoniae in a tertiary hospital in Fuzhou, China

2020 ◽  
Vol 129 (5) ◽  
pp. 1220-1226
Author(s):  
D. Chen ◽  
H. Li ◽  
Y. Zhao ◽  
Y. Qiu ◽  
L. Xiao ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Tertiary Hospital ◽  
Carbapenem Resistant

scholarly journals Molecular characterization of carbapenem resistant Klebsiella pneumoniae clinical isolates: Preliminary experience from a tertiary care teaching hospital in the Himalayas

2021 ◽  
Author(s):  
Mohit Bhatia ◽  
Varun Shamanna ◽  
Geetha Nagaraj ◽  
Dharmavaram Sravani ◽  
Pratima Gupta ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Molecular Characterization ◽  
Teaching Hospital ◽  
Tertiary Care ◽  
Resistant Bacteria ◽  
Central Research ◽  
Tertiary Care Teaching Hospital ◽  
Care Teaching ◽  
Carbapenem Resistant

Introduction: Klebsiella pneumoniae is recognized as an urgent threat to human health. Development of novel treatment options is the need of the hour. One of the ways of achieving this goal is by conducting molecular characterization studies of antibiotic resistant bacteria. Hypothesis/Gap Statement: Efforts are being made by the Indian government to perform molecular characterization of MDR and potentially virulent bacteria by whole-genome sequencing (WGS). However, the data that we have at present is skewed as many parts of the country remain underrepresented. Uttarakhand is one such state, with relatively poor access to healthcare and a paucity of research. Aim: To generate WGS based preliminary data about the population structure, multi-locus sequence types (MLST), and virulence factors of CRKp isolates recovered from patients in a tertiary care teaching hospital located in Uttarakhand, India. Methodology: A cross-sectional study was conducted which included twenty-nine randomly selected and archived carbapenem resistant Klebsiella pneumoniae (CR-Kp) isolates obtained from various clinical samples submitted in the Bacteriology laboratory for culture and sensitivity testing, from July 2018 to August 2019. After preliminary identification (ID) and antibiotic susceptibility testing (AST), as per standard guidelines, these isolates were sent to Central Research Laboratory (CRL), Bengaluru, India, for further characterization & WGS. Results: 27 out of 29 test isolates were CRKp. Among the 27 CRKp isolates, ST14 was the most common sequence type (8, 29.6%), followed by ST231 (5, 18.5%) and ST147(3, 11.1%) respectively. KL2 (9/27, 33.3%) and KL51 (8/27, 29.6%) were dominant K loci types in this study. O1 and O2 together accounted for 88.9% (n=27) CRKp isolates. Yersiniabactin and Aerobactin were identified in 88.9% (24/27) & 29.6% (8/27) of the CRKp isolates of the isolate. The predominant plasmid replicons present were ColKP3 (55.5%), IncFII(K) (51.8%), IncFIB(pQil) (44.4%), IncFIB(K) (37%), IncR (33.3%) and Col44 0I (18.5%) respectively. As compared to phenotypic resistance, higher genotypic resistance for aminoglycosides (96.3%) and folate pathway inhibitors (92.6%) respectively, was observed. Conclusion: This study emphasizes the need for continued genomic surveillance of emerging CRKp and other MDR bacteria in Uttarakhand and neighbouring states of India.

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