scholarly journals Meat Species Identification using Loop-mediated Isothermal Amplification Assay Targeting Species-specific Mitochondrial DNA

2014 ◽  
Vol 34 (6) ◽  
pp. 799-807 ◽  
Author(s):  
Ae-Ri Cho ◽  
Hee-Jin Dong ◽  
Seongbeom Cho
Keyword(s):  
Mitochondrial Dna ◽  
Species Identification ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification ◽  
Meat Species Identification ◽  
Amplification Assay ◽  
Meat Species ◽  
Species Specific

scholarly journals Meat species identification and Halal authentication analysis using mitochondrial DNA

Meat Science ◽  
2009 ◽  
Vol 83 (1) ◽  
pp. 57-61 ◽  
Author(s):  
Chandrika Murugaiah ◽  
Zainon Mohd Noor ◽  
Maimunah Mastakim ◽  
Lesley Maurice Bilung ◽  
Jinap Selamat ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Species Identification ◽  
Meat Species Identification ◽  
Meat Species ◽  
Halal Authentication

Identification for adulteration of beef with chicken based on single primer-triggered isothermal amplification

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Jiao Chen ◽  
Pansong Zhang ◽  
Haixia Wang ◽  
Yanjing Shi
Keyword(s):  
Limit Of Detection ◽  
Chicken Meat ◽  
Isothermal Amplification ◽  
Work Flow ◽  
Acid Extraction ◽  
Specificity And Sensitivity ◽  
Total Dna ◽  
Meat Species ◽  
Species Specific ◽  
Single Primer

Abstract Adulteration of beef with cheap chicken has become a growing problem worldwide. In this study, a quick, single primer-triggered isothermal amplification (SAMP) combined with a fast nucleic acid extraction method was employed to detect the chicken meat in adulterated beef. Chicken from adulterated beef was identified using the chicken species-specific primer designed according to the Gallus gallus mitochondrial conserved sequences. Our SAMP method displayed good specificity and sensitivity in detecting chicken and beef meat DNA–the limit of detection (LOD) of SAMP is 0.33 pg/μL of chicken and beef total DNA and 2% w/w chicken meat in beef. The whole work flow from DNA extraction to signal detection can be finished within 1 h, fulfilling the requirement of on-site meat species identification.

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