Systemic Tropheryma whippleii Infection Associated With Monoclonal B-Cell Proliferation: A Helicobacter pylori–Type Pathogenesis?

2003 ◽  
Vol 127 (12) ◽  
pp. 1619-1622
Author(s):  
Sa Wang ◽  
Linda M. Ernst ◽  
Brian R. Smith ◽  
Giovanni Tallini ◽  
John G. Howe ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Cell Proliferation ◽  
Lymph Node ◽  
B Cells ◽  
Lymph Nodes ◽  
B Cell ◽  
Chain Reaction ◽  
Mesenteric Lymph ◽  
Whipple Disease ◽  
Polymerase Chain

Abstract We report a case of Whipple disease in a 55-year-old woman who presented with arthralgia, weight loss, and lymphadenopathy. Tropheryma whippleii bacilli were identified in the mesenteric lymph nodes by diastase-resistant periodic acid–Schiff stain and confirmed by electron microscopy. Retrospectively, previous biopsy specimens from the duodenum and right axillary lymph node of this patient, which were initially considered to demonstrate reactive changes, also showed features consistent with involvement by Whipple disease. At the time of presentation, a large κ-restricted monoclonal B-cell population with the phenotype CD20+CD19+CD5−CD10− was identified in the patient's peripheral blood, lymph nodes, and bone marrow by flow cytometry study. The monoclonality of the mesenteric lymph node B cells was confirmed by immunohistochemical stain for κ chain after antigen retrieval and also by polymerase chain reaction with the primer set targeting FR2-VH. Routine cytogenetic study failed to reveal any chromosomal abnormalities, and polymerase chain reaction for Bcl-2 major and minor breakpoint cluster of t(14:18) was not detected. The monoclonal B cells have persisted in blood for the entire follow-up period (10 months). The possibility of reactive monoclonal B-cell proliferation versus Whipple disease–related B-cell lymphoma is discussed.

Determination of B-Cell Clonality in Paraffin-Embedded Lymph Nodes Using the Polymerase Chain Reaction

1993 ◽  
Vol 2 (1) ◽  
pp. 42-49 ◽  
Author(s):  
Thomas J. Reed ◽  
Ann Reid ◽  
Karen Wallberg ◽  
Timothy J. O??Leary ◽  
Glauco Frizzera
Keyword(s):  
Polymerase Chain Reaction ◽  
Lymph Nodes ◽  
B Cell ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Cell Clonality

scholarly journals Rearrangement and selection of VH11 in the Ly-1 B cell lineage.

1990 ◽  
Vol 172 (1) ◽  
pp. 371-374 ◽  
Author(s):  
C E Carmack ◽  
S A Shinton ◽  
K Hayakawa ◽  
R R Hardy
Keyword(s):  
Polymerase Chain Reaction ◽  
B Cells ◽  
B Cell ◽  
Peritoneal Cavity ◽  
Cell Lineage ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Vh Genes ◽  
Self Antigen ◽  
Selection Of

One of the predominant VH genes utilized to encode the anti-BrMRBC specificity is a member of the small VH11 family rearranged to JH1. Using the polymerase chain reaction (PCR) we have determined that the frequency of B cells with a VH11 rearrangement is 10-20 times higher in Ly-1 B than in Ly-1- "conventional" B cells regardless of location (spleen or peritoneal cavity). Conventional B cells rearrange this gene at comparable levels in pre-B cells and in mature B cells utilizing all JH gene segments. In contrast, the increased levels of VH11 rearrangement in Ly-1 B are restricted to JH1 (and some JH2) and therefore appear to be the result of selection. Furthermore, most peritoneal Ly-1 B cells with VH11 rearrangements fall in a fraction stained by anti-BrMRBC antibody, likely bearing multivalent natural (likely self) antigen constitutively bound to their surface Ig receptors. Thus, we suggest that autoantigens are largely responsible for the accumulation of autoantibody specificities in the Ly-1 B cell lineage with time, whereas they do not exert this effect in the conventional B cells.

Immunohistochemistry and Reverse Transcriptase Polymerase Chain Reaction on Sentinel Lymph Nodes can Improve the Accuracy of Nodal Staging in Breast Cancer Patients

2001 ◽  
Vol 16 (4) ◽  
pp. 227-232 ◽  
Author(s):  
G. Péley ◽  
J. Tóth ◽  
O. Csuka ◽  
I. Sinkovics ◽  
E. Farkas ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Polymerase Chain Reaction ◽  
Lymph Node ◽  
Lymph Nodes ◽  
Reverse Transcriptase ◽  
Sentinel Lymph Nodes ◽  
Nodal Staging ◽  
Rt Pcr ◽  
Chain Reaction ◽  
Polymerase Chain

In this study the nodal staging sensitivity of sentinel lymph node biopsy (SLNB) with detailed pathological and molecular biological examination has been investigated and compared to that of axillary lymph node dissection (ALND) with routine histological evaluation. Sentinel lymph nodes (SLNs) were removed by the dual-agent injection technique in 68 patients with primary, clinically node-negative breast cancer. Forty-seven patients had negative SLNs according to hematoxylin and eosin (H&E) staining. These H&E-negative SLNs were serially sectioned and examined at 250 μm levels by anticytokeratin immunohistochemistry (IHC). In 14 patients the SLNs were also investigated by cytokeratin 20 (CK20) reverse transcriptase polymerase chain reaction (RT-PCR). SLNB with IHC increased the node-positive rate by 26% (by 40% in tumors less than or equal to 2 cm in size (pT1) and by 9% in tumors more than 2 cm but less than or equal to 5 cm in size (pT2)). The sensitivity of SLNB with IHC was superior to that of ALND with routine histology in pT1 tumors and identical in pT2 tumors. The concordance between histology and RT-PCR was only 21%, and in two of three cases with positive histological results RT-PCR was negative. In conclusion, SLNB with detailed pathological and/or molecular biological evaluation can improve the sensitivity of regional staging. ALND can probably be abandoned in patients with pT1 SLN-negative breast cancer. Further prospective studies are required to determine the clinical significance of these detailed SLN evaluation techniques, but at present these methods are still investigational.

Determination of B-Cell Clonality in Paraffin-Embedded Lymph Nodes Using the Polymerase Chain Reaction

1993 ◽  
Vol 2 (1) ◽  
pp. 42-49 ◽  
Author(s):  
Thomas J. Reed ◽  
Ann Reid ◽  
Karen Wallberg ◽  
Timothy J. O??Leary ◽  
Glauco Frizzera
Keyword(s):  
Polymerase Chain Reaction ◽  
Lymph Nodes ◽  
B Cell ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Cell Clonality

scholarly journals Severe combined immunodeficiency (SCID) in man: B cell-negative (B-) SCID patients exhibit an irregular recombination pattern at the JH locus.

1991 ◽  
Vol 174 (5) ◽  
pp. 1039-1048 ◽  
Author(s):  
K Schwarz ◽  
T E Hansen-Hagge ◽  
C Knobloch ◽  
W Friedrich ◽  
E Kleihauer ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
B Cells ◽  
B Cell ◽  
Heavy Chain ◽  
Polymerase Chain Reaction Assay ◽  
Severe Combined Immunodeficiency ◽  
Combined Immunodeficiency ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
Healthy Persons

Human severe combined immunodeficiency (SCID) patients were analyzed by a polymerase chain reaction assay for their recombination capability at the DHQ52-JH region of the immunoglobulin heavy chain locus. Five patients with B cells (B+ SCID) exhibited a recombination pattern also observed in healthy persons. In contrast, six patients lacking B cells (B- SCID) showed a grossly altered rearrangement pattern characterized by the (partial) absence of regular DHQ52-JH recombinations and the presence of abnormal rearrangements. These events were caused by deletions surpassing the boundaries of immunoglobulin coding elements and thus resemble the pattern of deletional recombinations previously described in SCID mice.

scholarly journals Aberrant Cytokeratin 20 mRNA Expression in Peripheral Blood and Lymph Nodes Indicates Micrometastasis and Poor Prognosis in Patients With Gastric Carcinoma

2019 ◽  
Vol 18 ◽  
pp. 153303381983285
Author(s):  
Xiaolan You ◽  
Yuanjie Wang ◽  
Jian Wu ◽  
Qinghong Liu ◽  
Dehu Chen ◽  
...  
Keyword(s):  
Gastric Cancer ◽  
Polymerase Chain Reaction ◽  
Lymph Node ◽  
Lymph Nodes ◽  
Real Time ◽  
Peripheral Blood ◽  
Messenger Rna ◽  
Cytokeratin 20 ◽  
Chain Reaction ◽  
Polymerase Chain

Several studies suggest that peripheral blood and lymph node micrometastases may be a causative factor for gastric cancer recurrence. Cytokeratin 20 shows enriched expression in intestinal epithelial cells. This study aimed to evaluate the clinical utility of monitoring cytokeratin 20 levels in peripheral blood and lymph nodes of patients with gastric cancer for detecting micrometastasis and predicting prognosis. We detected messenger RNA levels of cytokeratin 20 in gastric cancer cell lines and in the peripheral blood of 125 patients (85 patients with gastric cancer and 40 patients with benign neoplasm) by fluorescence quantitative real-time polymerase chain reaction both before and after radical resection. In all, 1586 lymph node samples from 85 patients with gastric cancer were evaluated for cytokeratin 20 expression using real-time polymerase chain reaction, as well as by immunohistochemistry staining with anti-pan-keratin and anti-cytokeratin 20 antibodies. All patients underwent follow-up until cancer-related death or for more than 3 years after tumor resection. We found that elevated cytokeratin 20 expression in peripheral blood as detected by quantitative real-time polymerase chain reaction closely correlates with poor clinicopathological characteristics. Detecting cytokeratin 20 messenger RNA in the lymph nodes by quantitative real-time polymerase chain reaction enabled more accurate determination of the clinicopathological staging of gastric cancer, best treatment approach, and prognosis. Our findings show that patients with increased cytokeratin 20 messenger RNA expression in the peripheral blood or lymph nodes have a shorter time to recurrence and poorer overall survival.

Massive Visceral Pentastomiasis Caused by Porocephalus crotali in a Dog

2009 ◽  
Vol 46 (3) ◽  
pp. 460-463 ◽  
Author(s):  
M. D. Brookins ◽  
J. F. X. Wellehan ◽  
J. F. Roberts ◽  
K. Allison ◽  
S. S. Curran ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Lymph Nodes ◽  
18S Rrna ◽  
Reference Sequence ◽  
Intermediate Hosts ◽  
Mesenteric Lymph Nodes ◽  
Chain Reaction ◽  
Mesenteric Lymph ◽  
Pcr Product ◽  
Polymerase Chain

The testes of a 5-year-old, male, crossbred Schnauzer dog were the indicator organs for detection of massive pentastomiasis. Necropsy revealed numerous additional encysted parasites within the mesenteric lymph nodes, omentum, liver, sub-serosa of the small and large intestines, mesentery, and lungs. The nymphs had a pseudosegmented body, containing large eosinophilic glands and a chitinous cuticle with characteristic pores. Their hook configuration was consistent with that of Porocephalus. A pentastomid-specific 18S rRNA polymerase chain reaction (PCR) was designed and used to amplify template for sequencing. The sequence of the PCR product was 99.7% homologous with the reference sequence for P. crotali. This pentastomid parasite has been reported in North American snakes of genera Crotalus and Agkistrodon. Mammals are intermediate hosts, and snakes are the definitive hosts. Porocephalus crotali has been reported in dogs only once, and molecular methods have not been used previously to identify the species in clinical pentastomiasis.

scholarly journals The Effect of Curcumin on an Animal Intestinal Ischemia/Reperfusion Model for Bacterial Translocation and Inflammatory Response

2015 ◽  
Vol 100 (11-12) ◽  
pp. 1352-1359
Author(s):  
Selim Sözen ◽  
Mehmet Aziret ◽  
İlhan Bali ◽  
Seyfi Emir ◽  
Yiğit Ülgen ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Lymph Node ◽  
Inflammatory Response ◽  
Bacterial Translocation ◽  
Mesenteric Lymph Node ◽  
Ischemia Reperfusion ◽  
Chain Reaction ◽  
Blood Samples ◽  
Mesenteric Lymph ◽  
Polymerase Chain

Ischemia/reperfusion (IR) injury of the intestine is a major problem in abdominal pathological condition and is associated with a high morbidity and mortality. The purpose of the study is to investigate the effects of curcumin on the bacterial translocation incidence and inflammatory response in rats submitted to bowel ischemia reperfusion injury. Thirty-two Wistar albino rats with a weight of 200 to 250 g were used in the study. They were randomly divided into 3 groups (n = 10 for each group): sham only operated group(group I); IR group (group II); and IR + curcumin treatment group (group III). Curcumin (curcumin from Curcuma longa) 20 mg/kg/day was given orally to the curcumin group. All animals were given 109 E. Coli by orogastric intubation 12 hours before sampling. Seventy-two hours after the first operation, mesenteric lymph node and blood samples were obtained and cultured. Blood samples of 2 mL were obtained for a polymerase chain reaction study. A piece of terminal ileum was also sampled for histopathologic examination. Mesenteric lymph node and blood cultures of all control animals were positive for microbiological growth, and polymerase chain reaction results were positive in seven of the eight rats. Histopathologically, edema, vasodilatation and inflammatory cell infiltration were found to be less in the other groups in comparison to the control group. Curcumin reduced bacterial translocation in blood, hepatocellular damage, and plasma cytokine levels. Curcumin reduced the incidence of bacterial translocation in intestinal I/R. rats. These results suggest that Curcumin would be clinically useful in the treatment of intestinal I/R injury.

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