Basal/Myoepithelial Cells in Chronic Sinusitis, Respiratory Epithelial Adenomatoid Hamartoma, Inverted Papilloma, and Intestinal-Type and Nonintestinal-Type Sinonasal Adenocarcinoma: An Immunohistochemical Study

2007 ◽  
Vol 131 (4) ◽  
pp. 530-537 ◽  
Author(s):  
John A. Ozolek ◽  
E. Leon Barnes ◽  
Jennifer L. Hunt
Keyword(s):  
Proliferative Activity ◽  
Chronic Sinusitis ◽  
S100 Protein ◽  
Myoepithelial Cells ◽  
Inverted Papilloma ◽  
Proliferation Index ◽  
Ki 67 ◽  
Respiratory Epithelial Adenomatoid Hamartoma ◽  
Sinonasal Adenocarcinoma ◽  
Respiratory Epithelial

Abstract Context.—The pathogenesis of respiratory epithelial adenomatoid hamartoma (REAH) and inverted papilloma (IP) is poorly understood, especially compared with sinonasal adenocarcinoma (SNAC). One feature of malignant glandular lesions is loss of the basal/myoepithelial layer. The immunophenotype of the basal/myoepithelial layer has not been fully examined in benign glandular lesions of the sinonasal tract. Objective.—To examine benign and malignant glandular lesions in the sinonasal tract for the immunophenotype of basal/myoepithelial cells, proliferation index, and cytokeratin and intestinal differentiation profiles. Design.—Sinonasal adenocarcinoma (intestinal-type adenocarcinoma [ITAC] and nonintestinal type adenocarcinoma [non-ITAC]), REAH, IP, and chronic sinusitis (CS) were stained for cytokeratin (CK) 7, CK20, 34βE12, CDX-2, p63, Ki-67, smooth muscle actin (SMA), S100 protein, and calponin. Results.—Basal/myoepithelial cells in CS and REAH were positive for p63 and 34βE12 but negative for SMA, S100 protein, and calponin. Proliferative activity was localized to the compartment containing p63-positive cells. Inverted papilloma demonstrated broad areas staining for p63 and 34βE12, with intermediate proliferative activity in these areas. Sinonasal adenocarcinoma had the highest Ki-67 labeling index, and p63-positive SNACs had higher proliferation indices than p63-negative SNACs. REAH, IP, CS, and most SNACs expressed CK7. Only SNAC expressed CK20. Sixty percent of morphologic ITACs expressed CDX-2. Conclusions.—Basal/myoepithelial cells in CS and REAH should be considered basal and not myoepithelial cells. In benign lesions, proliferative activity is limited to the compartments with p63 staining. In SNAC and IP, p63 expression correlates with proliferation index. REAH, IP, and CS share similar immunoprofiles (CK7+, CK20−, and CDX-2−), contrasting with SNAC (CK7+, CK20+/−, CDX-2−/+).

scholarly journals Mandibular odontogenic sarcoma (ameloblastic fibrodentinosarcoma) in an aged cat — Short communication

2017 ◽  
Vol 65 (1) ◽  
pp. 89-95
Author(s):  
Gustavo A. Ramírez ◽  
Lorenzo Ressel ◽  
Jaume Altimira ◽  
Miquel Vilafranca
Keyword(s):  
Alveolar Bone ◽  
S100 Protein ◽  
Final Diagnosis ◽  
Proliferation Index ◽  
Cell Component ◽  
Ki 67 ◽  
Histologic Subtype ◽  
Odontogenic Tumours ◽  
Odontogenic Epithelium ◽  
Shaped Cell

A 13-year-old male cat presented with an ill-defined mass in the rostral mandible causing destruction and loss of alveolar bone. Microscopically, the mass consisted of cords or islands of benign odontogenic epithelium and a malignant, pleomorphic spindle-shaped cell component with dysplastic dentine formation. Immunohistochemically, neoplastic mesenchymal cells proved to be strongly positive for vimentin and negative for cytokeratins, desmin, actin and S100 protein; the Ki67 proliferation index was high. Morphological and immunohistochemical features largely overlap those reported for ameloblastic fibrodentinosarcoma, an uncommon histologic subtype of odontogenic sarcoma recognised in humans but no reported previously in animals. Ki-67 expression assessment may help to discriminate between malignant and benign forms of odontogenic tumours but the final diagnosis is mainly morphological.

scholarly journals Respiratory epithelial adenomatoid hamartoma: a diagnostic challenge in sinonasal lesions

2019 ◽  
Vol 12 (8) ◽  
pp. e230082
Author(s):  
Shailesh Ramesh Agrawal ◽  
Anagha Atul Joshi ◽  
Nikhil Dhorje ◽  
Renuka Bradoo
Keyword(s):  
Nasal Cavity ◽  
Nasal Septum ◽  
Low Grade ◽  
Diagnostic Challenge ◽  
Posterior Aspect ◽  
Respiratory Epithelial Adenomatoid Hamartoma ◽  
Complete Surgical Resection ◽  
Sinonasal Adenocarcinoma ◽  
Rare Lesion ◽  
Respiratory Epithelial

Respiratory epithelial adenomatoid hamartoma (REAH) is a rare lesion in nasal cavity first reported by Wenig and Heffner in 1995. Most commonly seen in men in third to ninth decade of life. Majority of cases presents as a polypoidal mass in one or both nasal cavities. We experienced such a case of REAH originating from the nasal septum, in posterior aspect, treated by endoscopic approach. It is important to differentiate REAH from other sinonasal pathologies like inverted papilloma and low grade sinonasal adenocarcinoma. Complete surgical resection is the treatment of choice.

scholarly journals A Case of Respiratory Epithelial Adenomatoid Hamartoma (REAH) in a Patient with History of Radiation Exposure

2019 ◽  
Vol 2019 ◽  
pp. 1-3
Author(s):  
Darshan N. Patel ◽  
Yekaterina A. Koshkareva ◽  
Miles McFarland ◽  
Nathan A. Deckard
Keyword(s):  
Radiation Exposure ◽  
Inverted Papilloma ◽  
Respiratory Epithelial Adenomatoid Hamartoma ◽  
Histological Appearance ◽  
History Of ◽  
Patient’S History ◽  
Respiratory Epithelial

Respiratory epithelial adenomatoid hamartomas (REAHs) are becoming a more commonly recognized otolaryngologic tumor and are often misdiagnosed as inverted papilloma. Here, we present such a case in a patient with history of previous radiation exposure. Otolaryngologists and pathologists should be aware of the mucinous histological appearance of REAH to help differentiate from other growths. Given our patient’s history, an association between REAH and previous radiation exposure is worth consideration.

scholarly journals Correlation of 11C-Methionine Combined Positron Emission and Computed Tomography and Ki-67 Proliferation Index in Pretreatment Assessment of Cerebral Gliomas

2021 ◽  
pp. 34-48
Author(s):  
T. Yu. Skvortsova ◽  
Zh. I. Savintceva ◽  
D. V. Zakhs ◽  
A. F. Gurchin ◽  
A. I. Kholyavin ◽  
...  
Keyword(s):  
Computed Tomography ◽  
Proliferative Activity ◽  
Hot Spot ◽  
Proliferation Index ◽  
Low Grade ◽  
Normal Brain ◽  
Ki 67 ◽  
Positron Emission ◽  
Pet Ct ◽  
Cerebral Gliomas

The purpose of the study was to explore the correlation between 11С-methionine (Met) uptake measured by combined positron emission and computed tomography (PET/CT) in newly diagnosed cerebral gliomas and tumor proliferative activity as measured by Ki-67 labeling index (Ki-67 LI).The results of PET/CT with 11С-methionine (PET-Met) of 236 adult patients with pretreated glial brain tumors were included in retrospective analysis. The final diagnosis of glioma according to WHO classification of CNS tumors (2007) was based on both histology and immunohistochemistry using Ki-67 antibodies. On PET-Met tumor-to normal brain uptake ratio (TBR) was calculated by dividing maximum Met uptake in the tumor (hot spot 10 mm in diameter) to activity concentration in the contralateral cortex. The Spearmen rank correlation test was used to analyze the relationships between TBR and Ki-67 LI.PET-Met analysis showed that TBR increases with an increase in the aggressiveness of the glial tumor. The differences of TBR values between gliomas grade II vs III and grade III vs IV were significant (p < 0,001). Among grades II-III gliomas Met uptake was significantly higher in oligodendroglial and mixed gliomas than in astrocytomas (p < 0,001), but the differences did not depend on Ki-67 LI.Correlation analysis demonstrated significant correlation between Ki-67 LI and TBR values (r = 0,49, p < 0,05, Spearman rank test). With analyzing glioma subgroups TBR values correlated with Ki-67 LI in diffuse astrocytomas (r = 0,52, p < 0,05), oligodendrogliomas (r = 0,40, p < 0,05), oligoastrocytomas (r = 0,47, p < 0,05) and in high-grade gliomas (r = 0,45, p < 0,05) but not in low-grade gliomas. Comparison between TBR value and Ki-67 LI in each glioma showed a lack of coincidence in 22 % of cases (high Met uptake but low Ki-67 LI and vice versa). The main reasons for such discrepancies were tumor molecular biology or incorrect biopsy target.Met uptake in diffuse gliomas correlates with proliferative activity which justifies the use of PET-Met for glioma grading. In case of mismatch between two biomarkers one should rely on the indicator that implies a higher aggressiveness of the glioma.

scholarly journals Proliferation Index and Karyometric Features of Pancreatic Intraductal Proliferative Lesions

1999 ◽  
Vol 19 (3-4) ◽  
pp. 175-185 ◽  
Author(s):  
Romana Tomaszewska ◽  
Krzysztof Okoń ◽  
Krystyna Nowak ◽  
Jerzy Stachura
Keyword(s):  
Pancreatic Cancer ◽  
Proliferative Activity ◽  
Diagnostic Value ◽  
Pancreatic Disease ◽  
Proliferation Index ◽  
Low Grade ◽  
Cell Nuclei ◽  
Ki 67 ◽  
Single Feature ◽  
Papillary Hyperplasia

The increasing frequency and poor prognosis in pancreatic cancer prompt us to search for morphological lesions being a substrate for its development. Studies of autopsy and surgically resected material as well as recent molecular studies have proved that one of the possible pathways of pancreatic neoplasia is the intraepithelial proliferation – dysplasia – cancer sequence. In the present paper we studied the proliferative activity (Ki‐67 index) in pancreatic intraepithelial proliferative lesions and its correlation with geometric features of cell nuclei as signs of increasing dysplasia. The studies were carried out in a group of 35 patients operated on for pancreatic cancer, chronic pancreatitis and other conditions not associated with the pancreas. We used immunohistochemical methods and basic morphometric parameters. The results of our studies indicate that the cell proliferative activity depends both on the type of epithelial proliferation and underlying pancreatic disease. The values of Ki‐67 index are significantly different in low‐grade proliferation (flat and papillary hyperplasia) and high‐grade proliferation (atypical papillary hyperplasia and carcinomain situ). A set of karyometric features correlates with Ki‐67 index but there is no single feature which would have a diagnostic value.

scholarly journals Proliferative activity of colorectal adenocarcinoma: objectification of criteria of morphological evaluation

2018 ◽  
Vol 18 (3) ◽  
pp. 52-56
Author(s):  
D P Kovtun ◽  
N M Anichkov ◽  
O G Polushin ◽  
E V Ponomaryeva ◽  
A I Lyubimov ◽  
...  
Keyword(s):  
Proliferative Activity ◽  
Rank Correlation ◽  
Immunohistochemical Method ◽  
Nuclear Antigen ◽  
Proliferation Index ◽  
Ki 67 ◽  
Cloud Clusters ◽  
Kendall Rank Correlation ◽  
Colon And Rectum ◽  
High Degree

Aim of the study. To analyze the correlation between proliferative activities determined on the basis of routine histological staining and immunohistochemical reaction to the nuclear antigen Ki-67 in colorectal adenocarcinomas. Methods. Thirty adenocarcinomas of the colon and rectum were retrospectively evaluated. Evaluation of proliferative activity was performed on serial histological preparations stained with hematoxylin and eosin and treated with antibodies to Ki-67. Calculation was carried out in the “hot spots” under four microscope high power fields (≈1 mm2). Results. An estimate of the Kendall rank correlation coefficient demonstrated a high degree of direct relationship between the number of mitoses counted by routine staining and proliferative activity estimated by the immunohistochemical method (τ = 0.708, p < 0.05). All observations on the dispersion diagram could be divided into three “cloud” clusters with similar values for Ki-67 with practically the same number of mitoses. Conclusions. On the basis of routine histological examination, it is possible to separate colorectal carcinomas from tumors with a low (1-2/mm2), moderate (3-5/mm2), and high (≥6/mm2) mitotic activity corresponding to the proliferation index values of Ki-67 <30%, 30%-50%, and >50%, respectively.

A case of synchronous hepatocellular carcinoma and aortic body chemodectoma in a dog — Pathological case report

2011 ◽  
Vol 59 (1) ◽  
pp. 113-121
Author(s):  
Ágnes Sterczer ◽  
Tibor Németh ◽  
Míra Mándoki ◽  
Péter Gálfi ◽  
Csaba Jakab
Keyword(s):  
Hepatocellular Carcinoma ◽  
Neuroendocrine Tumour ◽  
Primary Liver Cancer ◽  
S100 Protein ◽  
Neuron Specific Enolase ◽  
Lateral Lobe ◽  
Distant Metastases ◽  
Primary Hepatocellular Carcinoma ◽  
Proliferation Index ◽  
Ki 67

The authors describe a case of synchronously occurring (double) tumours, i.e. primary hepatocellular carcinoma and aortic body chemodectoma in a 14-year-old mixed-breed male dog. The tumours were identified during necropsy, following euthanasia. In the last months of its life, the dog showed signs of weakness, anorexia, apathy, inactivity, and abdominal palpation elicited a painful reaction. The primary liver cancer emerged in the left lateral lobe without evidence of any distant metastases. Histopathological and immunohistochemical investigations revealed a well-differentiated, trabecular, claudin-7-, claudin-5- and pancytokeratin-negative hepatocellular carcinoma. The Ki-67 proliferation index was 33%. During necropsy, a synchronously occurring benign, grade I type aortic body chemodectoma was also detected in the dog. This neuroendocrine tumour showed chromogranin-, synaptophysin-, neuron-specific enolase- and S100 protein-positivity, and the Ki-67 proliferation index was 2%. The authors believe that this is the first description of synchronously occurring hepatocellular carcinoma and aortic body chemodectoma in a dog.

scholarly journals Markers of Lymphocytic-Macrophagal Infiltration and Their Association With the Receptor Phenotype and Proliferative Activity of Tumor Tissue in Various Molecular-Biological Types of Endometrial Cancer

2021 ◽  
Vol 5 (Supplement_1) ◽  
pp. A1026-A1026
Author(s):  
Lev M Berstein ◽  
Alexander O Ivantsov ◽  
Aglaya Iyevleva
Keyword(s):  
Endometrial Cancer ◽  
Proliferative Activity ◽  
Tumor Tissue ◽  
Practical Importance ◽  
Rank Correlation ◽  
Macrophage Infiltration ◽  
Proliferation Index ◽  
Molecular Profile ◽  
Ki 67 ◽  
Receptor Phenotype

Abstract Background and Aims: The last years were characterized by a shift from the former subdivision of endometrial cancer (EC) into two main types [1, 2] to modern molecular biological classifications of this disease [3-5]. The purpose of this investigation was an attempt to compare such prognostic indicators for EC as features of lymphocytic [6] and macrophage infiltration of tumor tissue with markers of its hormonal sensitivity (receptor phenotype) and the proliferation index Ki-67 [7], taking into account the molecular biological type of the disease. Materials and Methods: The study involved material from untreated patients with endometrial cancer (a total of 219 people). The average age of patients was close to 55-60 years. Using classification of Talhouk et al. [5] allowed to perform a search for POLE mutations, evaluate by IHC the expression of the oncoprotein p53 and MMR (mismatch-repair) proteins /MLH1, MSH2, MSH6 and PMS2/, and also identify the type of disease without a characteristic molecular profile (WCMP). The IHC method was also used to study the rate of estrogen (ER) and progesterone (PR) receptors, Ki-67 proliferative activity index, as well as the severity of macrophage-lymphocytic tissue infiltration of EC based on the analysis of the macrophage (CD68) and lymphocytic cells (cytotoxic CD8 and regulatory FoxP3) markers using reagents from Ventana and Dako. Statistical assessment of the relationships of the studied indicators was carried out by the Spearman rank correlation coefficient. Results: FoxP3 (in contrast to CD8 and CD68) positively and significantly correlates (ρ varies from 0.2895 to 0.3477) more often with ER, but not with PR. Ki-67 index in EC tissue positively and reliably correlates with FoxP3 both in the MMR-D and WCMP groups and in the combined cohort of EC patients. In the latter case, a similar relationship with Ki-67 extends to other studied markers of lymphocytic-macrophage infiltration, namely CD8 and CD68 (ρ 0,1746-0,3294). Only in the entire group of EC patients there is a positive rank correlation (0.4119!) between ER and PR expression. Conclusions: In patients with certain types of EC the connection between the estrogenic signal and PR induction is lost; it is especially noticeable in the MMR-D group, as exemplified by the negative correlation (-0.2951) of FoxP3 and PR expression. Taken together with existing data this indicates an important role of the endocrine component for differentiating separate groups of patients with EC, that may also be of practical importance. References: 1. Bokhman JV. Gynecol Oncol 1983; 15: 10-17. 2. Suarez AA et al. Gynecol Oncol 2017;144(2):243-249. 3. Murali R et al. Lancet Oncol 2014; 15: e268-278. 4.Berstein LM et al. Future Oncol. 2017 13(28):2593-2605. 5. Talhouk A. et al. Cancer. 2017;123(5):802-813. 6. Gargiulo P. et al. Cancer Treat Rev. 2016;48:61-8. 7. Kitson S. et al. Mod Pathol. 2017; 30(3): 459-468.

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