scholarly journals Characterization of Kenyan French Bean genotypes into gene pool affiliations using allele specific markers

2020 ◽  
Vol 19 (9) ◽  
pp. 653-660
Author(s):  
Esther Arunga Edith ◽  
Shadrack Odikara Oriama
Keyword(s):  
Gene Pool ◽  
French Bean ◽  
Allele Specific

scholarly journals Genetic diversity of common bean (Phaseolus vulgaris L.) breeding collection in Serbia

Genetika ◽  
2019 ◽  
Vol 51 (1) ◽  
pp. 1-15 ◽  
Author(s):  
Aleksandra Savic ◽  
Milka Brdar-Jokanovic ◽  
Miodrag Dimitrijevic ◽  
Sofija Petrovic ◽  
Milan Zdravkovic ◽  
...  
Keyword(s):  
Common Bean ◽  
Gene Pool ◽  
Gene Diversity ◽  
Principal Component ◽  
Molecular Data ◽  
Phenotypic Traits ◽  
Gene Pools ◽  
Vegetable Crops ◽  
Novi Sad

The characterization of 41 common bean cultivars and landraces from breeding collection of Institute of Field and Vegetable Crops, Novi Sad, Serbia, was done based on phenotypic traits and microsatellite markers. Phenotypic traits were chosen from Bioversity International descriptor list. In addition, main yield components were investigated. Analysis of phaseolin type revealed affiliation of cultivars and landraces to Mesoamerican or Andean gene pool. Cultivars and landraces demonstrated significant diversity level with regard to studied phenotypic traits. Identified variation showed high potential for developing new cultivars with desirable combination of traits. Principal component analysis based on phenotypic traits separated bean cultivars and landraces in two groups, which corresponded to Mesoamerican and Andean determined according to phaseolin type. Putative hybrids, with combination of traits between gene pools were also identified. Analysis of microsatellite data, using twenty-two SSR primer pairs, showed medium gene diversity in studied material. Microsatellite-based cluster analysis separated genotypes in two discrete clusters and several subclusters. No clear separation according to gene pool was found between the clusters, however grouping according to gene pool and patterns of phenotypic variation, following these gene pools, were observed within subclusters. Knowledge on detailed relationships of cultivars and landraces based on phenotypic and molecular data would facilitate identification of candidates for future breeding.

Purification and characterization of a constitutive polygalacturonase associated with the infection process of French bean leaves by Botrytis cinerea

1990 ◽  
Vol 68 (9) ◽  
pp. 1921-1930 ◽  
Author(s):  
G. Leone ◽  
E. A. M. Schoffelmeer ◽  
J. Van den Heuvel
Keyword(s):  
Botrytis Cinerea ◽  
Reaction Rate ◽  
French Bean ◽  
Infection Process ◽  
Purification Procedure ◽  
Purification And Characterization ◽  
Titration Curves ◽  
Optimal Ph ◽  
Bean Leaves

The constitutively produced polygalacturonase isoenzyme PG2 was isolated from culture filtrates of Botrytis cinerea, purified to homogeneity, and characterized. The shape of titration curves of PG2 and two other polygalacturonase isoenzymes explained the difficulties found in separating superimposed pectic enzyme activities during the purification procedure. PG2 hydrolyzed sodium polygalacturonate more quickly than pectin. The optimal pH for PG2 activity with polygalacturonate was 4.5 and with pectin, 4.0. PG2 activity was also influenced by the presence of NaCl or CaCl2 in the reaction mixture. Analysis of the breakdown products by paper chromatography and a comparison of the reaction rate by viscosimetry and reducing group assay revealed that PG2 has an endocatalytic mode of action on polygalacturonate. The isoelectric point and the molecular mass of PG2 were estimated to be 9.1 and 23.0 kDa, respectively. Key words: Botrytis cinerea, chromatofocusing, endopolygalacturonase, purification, substrate specificity, titration curve.

scholarly journals In vivo characterization of regulatory polymorphisms by allele-specific quantification of RNA polymerase loading

2003 ◽  
Vol 33 (4) ◽  
pp. 469-475 ◽  
Author(s):  
Julian C. Knight ◽  
Brendan J. Keating ◽  
Kirk A. Rockett ◽  
Dominic P. Kwiatkowski
Keyword(s):  
Rna Polymerase ◽  
Allele Specific ◽  
In Vivo Characterization

scholarly journals The S –locus and unilateral incompatibility

2003 ◽  
Vol 358 (1434) ◽  
pp. 1133-1140 ◽  
Author(s):  
C. Nathan Hancock ◽  
Katsuhiko Kondo ◽  
Brian Beecher ◽  
Bruce McClure
Keyword(s):  
Control Mechanisms ◽  
S Locus ◽  
Pollen Rejection ◽  
Stigma Surface ◽  
Allele Specific ◽  
Genetic Mechanisms ◽  
Active Research ◽  
Compatible Pollen ◽  
Identification And Characterization

Plants have many ways to regulate the type of pollen that arrives on the stigma surface. Once there, further control mechanisms regulate compatibility. The latter controls are largely based on biochemical interactions that support compatible pollination and prevent incompatible matings. S–RNase–based self–incompatibility (SI) systems are the most phylogenetically widespread mechanisms for controlling pollination. Studies of Nicotiana establish a firm link between SI and unilateral interspecific incompatibility. Although implicated in both inter– and intraspecific compatibility, S–RNase operates through at least three distinct genetic mechanisms that differ in their dependence on non–S–RNase factors. Identification and characterization of these non–S–RNase factors is currently an area of active research. Searching for genetic and biochemical interactions with S–RNase can identify candidate non–S–RNase factors. HT–protein is one factor that is required for S –allele–specific pollen rejection in the Solanaceae. Major style arabinogalactan proteins such as TTS interact biochemically with S–RNase. These glycoproteins are known to interact with compatible pollen tubes and have long been suggested as possible recognition molecules. Their binding to S–RNase implies a link between stylar systems for compatibility and incompatibility. Thus, genetic and biochemical studies suggest a highly networked picture of pollen–pistil interactions.

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