Molecular characterization of intestinal protozoan parasites from children facing diarrheal disease and associated risk factors in Yamoussoukro, Cte dIvoire

BackgroundIntestinal parasites are a major public health problem in the developing world and have attracted increasing levels of interest from health researchers over the past decade. Epidemiology-based studies have shown that the prevalence of intestinal parasites is high and they frequently recur in regions with poor sanitation and inadequate sewerage facilities. In this study, we determined the prevalence of intestinal parasites, their egg intensities per sample, and associated risk factors in an informal settlement.MethodsThis was a cross-sectional study conducted in three randomly selected public primary schools located in the informal settlements of Nakuru town. A total of 248 stool samples were collected from asymptomatic pupils and screened, using the Kato Katz technique, for infections caused by soil-transmitted helminths (STH). A random subset of stool samples (n=96) was also screened by polymerase chain reaction (PCR) to detect intestinal protozoa. Socio-demographic variables were collected using a pre-tested structured questionnaire; these data were analysed to identify risk factors for infection. ResultsThe overall prevalence of intestinal parasites was 17.3% (43/248 pupils). The overall prevalence of both STH and intestinal protozoan parasites was 1.2% and 41.7%, respectively. The most commonly diagnosed STH infection was Trichuris trichiura (1.2%), followed by hookworms (0.4%) and Ascaris lumbricoides (0.4%). The prevalence of intestinal protozoan parasites ranged from 0% to 38.5% and included Entamoeba histolytica, Entamoeba hartmanni, Entamoeba dispar, Giardia intestinalis, and Entamoeba coli. All infections were light, with an egg intensity <100 for each of the STH infections. The prevalence of multiple infections, including intestinal protozoan parasites, was 5.2% (n=5) and 0.4% (n=1) for STH in the subset samples. Finally, our analysis identified several significant risk factors for intestinal parasitic infections, including goat rearing (p=0.046), living in a home with an earthen floor (p=0.022), the number of rooms in the household (p=0.035), and the source of food (p=0.016). ConclusionThe low prevalence of intestinal parasites in the informal settlements of Nakuru may be attributed to improvements in hygiene and sanitation, deworming, and general good health practices that are facilitated by the Department of Public Health.

Download Full-text

Prevalence of Intestinal Protozoan Parasites and Associated Risk Factors among School Children in Merhabete District, Central Ethiopia

Journal of Parasitology Research ◽

10.1155/2021/9916456 ◽

2021 ◽

Vol 2021 ◽

pp. 1-7

Author(s):

Nigus Dagne ◽

Amir Alelign

Keyword(s):

Risk Factors ◽

School Children ◽

Parasitic Infection ◽

Parasitic Infections ◽

Protozoan Parasites ◽

Intestinal Protozoan ◽

Cross Sectional ◽

Tropical Regions ◽

Central Ethiopia ◽

Associated Risk Factors

Background. Intestinal protozoan parasitic infections remain one of the major public health problems in tropical regions especially, among developing countries such as Ethiopia. However, no enough epidemiological data is available in this respect in many parts of the country. Hence, this study was aimed at assessing the prevalence of intestinal protozoan parasitic infections and their associated risk factors among school children in Zeita village, Merhabete District, North Shewa Zone, Central Ethiopia. Methods. A cross-sectional parasitological survey was conducted from January to March 2020. A total of 280 children from Zeita primary school were selected using stratified random sampling techniques. Stool samples were collected and examined using direct wet mount, Formal-Ether concentration and Modified Zeihel-Neelsen staining techniques. Results. The overall prevalence of intestinal protozoan parasitic infection was found to be 46.8% (131/280). The three predominant protozoan parasites detected in this study were Entamoeba histolytica, Giardia lamblia, and Cryptosporidium parvum which were observed in 70 (25.2%), 54 (19.3%), and 7 (2.5%) of the study participants, respectively. Sociodemographic characteristics of the study subjects such as family occupation ( P = 0.028 ), sources of drinking water ( P = 0.001 ), water handling practice ( P = 0.027 ), habit of eating vegetable ( P = 0.001 ), and presence of latrine were observed to be significantly associated risk factors for the occurrence of human intestinal protozoan parasites. Conclusion. A high prevalence of intestinal protozoan parasitic infection which has been contributed by different risk factors was revealed in this study. The findings suggested a need of collaborative effort among the educational and health authorities to control the infection in the study area.

Download Full-text

Prevalence, risk factors of infection and molecular characterization of trichomonads in puppies from French breeding kennels

Veterinary Parasitology ◽

10.1016/j.vetpar.2013.07.030 ◽

2013 ◽

Vol 197 (3-4) ◽

pp. 418-426 ◽

Cited By ~ 12

Author(s):

Aurélien Grellet ◽

BrunoPolack ◽

Alexandre Feugier ◽

Corine Boucraut-Baralon ◽

Dominique Grandjean ◽

...

Keyword(s):

Risk Factors ◽

Molecular Characterization

Download Full-text

Prevalence, risk factors and molecular characterization of Enterocytozoon bieneusi in raccoon dogs (Nyctereutes procyonoides) in five provinces of Northern China

Acta Tropica ◽

10.1016/j.actatropica.2016.05.015 ◽

2016 ◽

Vol 161 ◽

pp. 68-72 ◽

Cited By ~ 13

Author(s):

Chao Xu ◽

Xusheng Ma ◽

Hao Zhang ◽

Xiao-Xuan Zhang ◽

Jia-Ping Zhao ◽

...

Keyword(s):

Risk Factors ◽

Molecular Characterization ◽

Northern China ◽

Enterocytozoon Bieneusi ◽

Nyctereutes Procyonoides ◽

Raccoon Dogs

Download Full-text

Prevalence and associated risk factors of human intestinal parasitic infections: a population-based study in the southeast of Kerman province, southeastern Iran

10.21203/rs.2.15697/v1 ◽

2019 ◽

Author(s):

Mohammad Javad Abbaszadeh Afshar ◽

Maryam Barkhori Mehni ◽

Mostafa Rezaeian ◽

Mehdi Mohebali ◽

Vali Baigi ◽

...

Keyword(s):

Risk Factors ◽

Intestinal Parasites ◽

Agar Plate ◽

Public Health Problem ◽

Effective Control ◽

Multiple Infections ◽

Parasitic Infections ◽

Intestinal Protozoan ◽

Intestinal Parasitic Infections ◽

Associated Risk Factors

Abstract Background Determination of the prevalence and distribution pattern of intestinal parasites is a fundamental step to set up an effective control program to improve the health status. This study aimed to determine the prevalence of intestinal parasitic infections and associated risk factors among inhabitants of Rudbar-e Jonub county, southeast of Kerman province, southeastern Iran.Methods In this cross-sectional study, 861 stool specimens were collected from inhabitants of Rudbar-e Jonub county through a multistage cluster sampling method in 2018. The collected specimens were examined by parasitological methods including, direct wet-mounting (for the fresh specimens with a watery consistency), formalin-ethyl acetate sedimentation and agar plate culture.Results The prevalence of intestinal parasites was 34.2% (95% CI 30.1 to 38.2). The prevalence of protozoan parasites 32.3% (95% CI 28.4 to 36.5) was significantly higher than helminthic parasites 3.2% (95% CI 2.1 to 4.7). Blastocystis sp. , Entamoeba coli and Giardia lamblia as protozoan parasite and Hymenolepis nana as helminthic parasite were the most common detected intestinal parasites in the study. Entamoeba histolytica/dispar , Iodamoeba bütschlii , Chilomastix mesnili , Entamoeba hartmanni , Ascaris lambercoides , and Enterobius vermicularis were other detected parasites. Multiple logistic regression revealed a significant association of intestinal parasitic infections with source of drinking water and residency status (rural/urban). Multiple infections with 2 or 3 parasitic agents constituted 22.7% of 295 infected cases.Conclusions This study revealed a high prevalence of intestinal protozoan infections among inhabitants of Rudbar-e Jonub county. Intestinal parasites especially protozoans remain a challenging public health problem wherever sanitation and health measures are limited in Iran.

Download Full-text