The behavior of Salmonella Typhimurium, Salmonella Typhi, Salmonella Agona and Salmonella Montevideo in raw carrot and in fresh unpasteurized carrot juice

Customizable PCR-microplate arrays were developed for the rapid identification of Salmonella Typhimurium, Salmonella Saintpaul, Salmonella Typhi, Shigella dysenteriae, Escherichia coli O157:H7, Francisella tularensis subsp. tularensis, Francisella tularensis subsp. novicida, Vibrio cholerae, Vibrio parahaemolyticus, Yersinia pestis, and Yersinia pseudotuberculosis. Previously, we identified highly specific primers targeting each of these pathogens. Here, we report the development of customizable PCR-microplate arrays for simultaneous identification of the pathogens using the primers identified. A mixed aliquot of genomic DNA from 38 strains was used to validate three PCR-microplate array formats. Identical PCR conditions were used to run all the samples on the three formats. Specific amplifications were obtained on all three custom plates. In preliminary tests performed to evaluate the sensitivity of these assays in samples inoculated in the laboratory with Salmonella Typhimurium, amplifications were obtained from 1 g of beef hot dog inoculated at as low as 9 CFU/ml or from milk inoculated at as low as 78 CFU/ml. Such microplate arrays could be valuable tools for initial identification or secondary confirmation of contamination by these pathogens.

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In-vitro Anti-bacterial Effects of Jatropha curcas on Salmonella Typhi and Salmonella Typhimurium Isolated from Presumptive Typhoid Fever Patients in Akure Metropolis, Nigeria

European Journal of Medicinal Plants ◽

10.9734/ejmp/2016/23308 ◽

2016 ◽

Vol 12 (2) ◽

pp. 1-10

Author(s):

O Ajayi ◽

S Awala ◽

F Okogbue ◽

A Ogunleye ◽

T Adeyeye

Keyword(s):

Typhoid Fever ◽

Salmonella Typhimurium ◽

Jatropha Curcas ◽

Salmonella Typhi

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Genomic Subtraction Identifies Salmonella typhimurium Prophages, F-Related Plasmid Sequences, and a Novel Fimbrial Operon, stf, Which Are Absent inSalmonella typhi

Journal of Bacteriology ◽

10.1128/jb.181.18.5652-5661.1999 ◽

1999 ◽

Vol 181 (18) ◽

pp. 5652-5661 ◽

Cited By ~ 36

Author(s):

Melanie Emmerth ◽

Werner Goebel ◽

Samuel I. Miller ◽

Christoph J. Hueck

Keyword(s):

Salmonella Typhimurium ◽

Virulent Strain ◽

Inbred Mice ◽

Subtractive Hybridization ◽

Salmonella Typhi ◽

Virulence Plasmid ◽

Sugar Transporters ◽

Subtraction Procedure ◽

Hybridization Procedure ◽

K 12

ABSTRACT Salmonella typhimurium causes systemic and fatal infection in inbred mice, while the related serotype Salmonella typhi is avirulent for mammals other than humans. In order to identify genes from the virulent strain S. typhimurium ATCC 14028 that are absent in S. typhi Ty2, and therefore might be involved in S. typhimurium mouse virulence, a PCR-supported genomic subtractive hybridization procedure was employed. We have identified a novel putative fimbrial operon,stfACDEFG, located at centisome 5 of the S. typhimurium chromosome, which is absent in S. typhi,Salmonella arizonae, and Salmonella bongori but was detected in several other Salmonella serotypes. The fimbrial genes represent a genomic insertion in S. typhimurium compared to the respective region betweenfhuB and hemL in Escherichia coliK-12. In addition, the subtraction procedure yielded F plasmid-related sequences from the S. typhimurium virulence plasmid, a number of DNA fragments representing parts of lambdoid prophages and putative sugar transporters, and several fragments with unknown sequences. The majority of subtracted chromosomal sequences map to three distinct locations, around centisomes 5, 27, and 57.

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Peer Review #1 of "Cell-free supernatants from cultures of lactic acid bacteria isolated from fermented grape as biocontrol against Salmonella Typhi and Salmonella Typhimurium virulence via autoinducer-2 and biofilm interference (v0.1)"

10.7287/peerj.7555v0.1/reviews/1 ◽

2019 ◽

Author(s):

MÁ Martínez-Téllez

Keyword(s):

Lactic Acid ◽

Lactic Acid Bacteria ◽

Salmonella Typhimurium ◽

Peer Review ◽

Salmonella Typhi ◽

Autoinducer 2

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Genomic Subtractive Hybridization and Selective Capture of Transcribed Sequences Identify a Novel Salmonella typhimurium Fimbrial Operon and Putative Transcriptional Regulator That Are Absent from the Salmonella typhi Genome

Infection and Immunity ◽

10.1128/iai.67.10.5106-5116.1999 ◽

1999 ◽

Vol 67 (10) ◽

pp. 5106-5116 ◽

Cited By ~ 37

Author(s):

Brian J. Morrow ◽

James E. Graham ◽

Roy Curtiss

Keyword(s):

Salmonella Typhimurium ◽

Transcriptional Regulator ◽

Subtractive Hybridization ◽

Salmonella Typhi ◽

Etiologic Agent ◽

Nucleotide Sequence Analysis ◽

Broad Host Range ◽

Macrophage Phagocytosis ◽

Cdna Nucleotide Sequence ◽

High Level

ABSTRACT Salmonella typhi, the etiologic agent of typhoid fever, is adapted to the human host and unable to infect nonprimate species. The genetic basis for host specificity in S. typhi is unknown. The avirulence of S. typhi in animal hosts may result from a lack of genes present in the broad-host-range pathogenSalmonella typhimurium. Genomic subtractive hybridization was successfully employed to isolate S. typhimurium genomic sequences which are absent from the S. typhi genome. These genomic subtracted sequences mapped to 17 regions distributed throughout the S. typhimurium chromosome. A positive cDNA selection method was then used to identify subtracted sequences which were transcribed by S. typhimurium following macrophage phagocytosis. A novel putative transcriptional regulator of the LysR family was identified as transcribed by intramacrophage S. typhimurium. This putative transcriptional regulator was absent from the genomes of the human-adapted serovars S. typhi andSalmonella paratyphi A. Mutations within this gene did not alter the level of S. typhimurium survival within macrophages or virulence within mice. A subtracted genomic fragment derived from the ferrichrome operon also hybridized to the intramacrophage cDNA. Nucleotide sequence analysis of S. typhimurium and S. typhi chromosomal sequences flanking the ferrichrome operon identified a novel S. typhimurium fimbrial operon with a high level of similarity to sequences encoding Proteus mirabilis mannose-resistant fimbriae. The novel fimbrial operon was absent from the S. typhi genome. The absence of specific genes may have allowedS. typhi to evolve as a highly invasive, systemic human pathogen.

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Frecuencia de bacterias gramnegativas en teléfonos celulares de estudiantes de enfermería

SANUS ◽

10.36789/sanus.vi11.145 ◽

2019 ◽

pp. 6-18

Author(s):

María Isabel Álvarez-Rangel ◽

Gerardo Flores-Patiño ◽

Itzen Lazarini-Torres ◽

Said Alejandro Cazares-Patiño ◽

Dolores Monserrat Silva-Camacho ◽

...

Keyword(s):

Escherichia Coli ◽

Klebsiella Pneumoniae ◽

Salmonella Typhimurium ◽

Enterobacter Aerogenes ◽

Salmonella Typhi ◽

Para Determinar

Introducción: Hoy en día, el uso indiscriminado del teléfono celular ha llevado a manipularlo en condiciones inadecuadas de higiene. Objetivo: Identificar la frecuencia de bacterias gramnegativas (Salmonella typhimurium, Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae y Pseudomona aeruginosa) en los teléfonos celulares de los estudiantes de la Licenciatura en Enfermería de una Universidad del centro de México. Metodología: El estudio corresponde a un enfoque cuantitativo, transversal y un alcance descriptivo. Se realizó un muestreo no probabilístico por conveniencia, eligiendo a 60 alumnos con previo consentimiento informado. Se tomaron las muestras en los teléfonos celulares, se procedió a la incubación por 24 horas en tubos con medio Soya Tripticaseína, se sembró en cajas petri, dejándose incubar por 48 horas y se procedió a la caracterización morfológica de las bacterias para determinar su presencia. Resultados: Del 100% de las muestras, el 41.67% no presentó crecimiento bacteriano y en el 58.33% de los teléfonos se obtuvieron los siguientes resultados: Salmonella Typhi con 2.98%, Enterobacter Aerogenes 28.35%, Escherichia Coli 28.35%, Klebsiella 11.94%, Pseudomona 0.00% y otras 28.35%. Conclusión: La mayoría de la muestra de estudio, porta en sus teléfonos celulares bacterias potencialmente patógenas, lo que supone un riesgo de contaminación cruzada y una posible fuente de brotes de infecciones intra y extrahospitalarias.

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