Effect of extracts of traditional Chinese medicines on anti-tyrosinase and antioxidant activities

Abstract Background Currently, although Inula nervosa Wall is well investigated, little is understood about blossoms of Inula nervosa Wall (BINW). Objective In this work, we systematically investigated the antioxidant activity of the extract from BINW by various standard assays including DPPH free radical ability, ABTS• +, and FRAP. Methods Chemical compounds are tentatively identified through an UHPLC-QTOF-MS system. Furthermore, the contents of nine compounds were detected with UHPLC method coupled with PDA. By carefully analyzing the quantitative data via clusters analysis and PCA. Results 46 compounds are tentatively identified and our results showed that 9 compound samples in 21 batches of BINW collected from different areas could be differentiated and analyzed by a heatmap visualization. In addition, the contents of nine compounds (flavonoids, phenolic acids) exhibited a total of higher amounts and better antioxidant activities from Yunnan than those from other three origins. Conclusions Our study not only developed a powerful platform to explain the difference traditional Chinese medicines (TCMs) species that in closely related through the chemometric and chemical profiling, but also presented a useful method to establish quality criteria of BINW with multiple origins. Highlights To detailly characterize the BINW, we not only performed DPPH, FRAP and ABTS assays to investigate its antioxidant activity, but also established UHPLC-QTOF-MS/MS and UHPLC-PDA based methods to comprehensively identify and qualitatively analyze its components.

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The Study of Steaming Durations and Temperatures on the Chemical Characterization, Neuroprotective, and Antioxidant Activities of Panax notoginseng

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2022/3698518 ◽

2022 ◽

Vol 2022 ◽

pp. 1-13

Author(s):

Didi Ma ◽

Jue Wang ◽

Guo Yin ◽

Lijun Wang ◽

Yibao Jin ◽

...

Keyword(s):

Antioxidant Activities ◽

Chemical Characterization ◽

Panax Notoginseng ◽

Absorption Capacity ◽

Traditional Chinese Medicines ◽

Chinese Medicines ◽

Hplc Fingerprint ◽

Relationship Analysis ◽

Antioxidant Effects ◽

Spectrum Effect

Panax notoginseng (PN) is one of the most valuable traditional Chinese medicines and has extensive pharmacological effects. Recent studies demonstrated that PN exhibited pharmacological effect related to Alzheimer’s disease (AD). However, whether steaming process can boost its anti-AD activity is still unexplored. To fill this gap, effects of steaming durations and temperatures on the chemical characterization, neuroprotective and antioxidant activities of PN were systematically investigated in this study. HPLC fingerprint coupled with quantitative analysis demonstrated striking conversion of original saponins to less polar ones with the increase in the steaming time and temperature. In the viewpoint of anti-AD activity on neuroprotective and antioxidant effects, several steamed PN samples (110°C-6/8/10 h, 120°C ‐4/6 h samples) displayed a significant increase both in cell viability and oxygen radical absorption capacity (ORAC) values compared with the no steamed one ( P < 0.01 or P < 0.005 ). Steaming temperature had the greater impact on the change of chemical composition and anti-AD activity of PN. Moreover, the spectrum-effect relationship analysis revealed that the transformed saponins were partially responsible for the increased neuroprotective and antioxidant effects of steamed PN. Therefore, steamed PN could be used as a potential crude drug for prevention and treatment of AD.

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Monitoring quality consistency of Ixeris sonchifolia (Bunge) Hance injection by integrating UV spectroscopic fingerprints, a multi-wavelength fusion fingerprint method, antioxidant activities and UHPLC/Q-TOF-MS

RSC Advances ◽

10.1039/c6ra16436f ◽

2016 ◽

Vol 6 (90) ◽

pp. 87616-87627 ◽

Cited By ~ 8

Author(s):

Lanping Yang ◽

Xiuman Xie ◽

Liu Yang ◽

Jing Zhang ◽

Guoxiang Sun

Keyword(s):

Antioxidant Activities ◽

Herbal Medicines ◽

Detection Technique ◽

Traditional Chinese Medicines ◽

Chinese Medicines ◽

Multi Wavelength ◽

Ixeris Sonchifolia ◽

Monitoring Quality ◽

Tof Ms

Traditional Chinese medicines/herbal medicines (TCM/HM) are too complicated mixture systems to investigate their quality consistency effectively using a single detection technique.

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