Effect of genotype, explant and culture media on direct plant regeneration in eggplant

2015 ◽  
Vol 72 (2) ◽  
pp. 232 ◽  
Author(s):  
Mohinder Kaur ◽  
Ajmer S. Dhatt ◽  
J.S. Sandhu ◽  
S.S. Gosal
Keyword(s):  
Plant Regeneration ◽  
Culture Media ◽  
Direct Plant Regeneration

scholarly journals Cell suspension culture and plant regeneration of a Brazilian plantain, cultivar Terra

2008 ◽  
Vol 43 (10) ◽  
pp. 1325-1330 ◽  
Author(s):  
Lucymeire Souza Morais-Lino ◽  
Janay Almeida dos Santos-Serejo ◽  
Sebastião de Oliveira e Silva ◽  
José Raniere Ferreira de Santana ◽  
Adilson Kenji Kobayashi
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Somatic Embryos ◽  
Culture Media ◽  
Ms Medium ◽  
Regenerated Plants ◽  
Male Flowers

The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no visible somaclonal variation was observed in vitro. It is possible to regenerate plants from cell suspensions of plantain banana cultivar Terra using MS medium supplemented with 11.4 µM of IAA and 2.2 µM of BAP.

In vitro culture of pointed gourd (Trichosanthes dioica Roxb.)

1970 ◽  
Vol 35 (1) ◽  
pp. 135-142 ◽  
Author(s):  
MA Malek ◽  
D Khanam ◽  
M Khatun ◽  
MH Molla ◽  
MA Mannan
Keyword(s):  
Plant Regeneration ◽  
In Vitro Culture ◽  
Culture Media ◽  
Culture Conditions ◽  
Root Induction ◽  
Ms Medium ◽  
Trichosanthes Dioica ◽  
Pointed Gourd ◽  
Regenerated Plantlets

An experiment was conducted to study the in vitro culture of pointed gourd. Cotyledon rescued from physiologically matured seeds (PMS) and immatured seeds (IMS) of pointed gourd were used as explants. Cotyledon excised from PMS responded very well in all culture conditions. Plant regenerated from cotyledon of PMS ranged from 38 to 96% in different hormonal formulations of culture media. Highest percentage of shoot regeneration was observed in MS + 1.0 mg/l BAP and lowest in MS + 2.5 mg/l BAP. No plant regeneration was observed in cotyledon from immatured seeds. The highest percentage of root induction (99%) was achieved in half MS medium supplemented with 0.5 mg/l NAA. The regenerated plantlets were successfully established in earthen pot. Keywords: Cotyledon; in vitro; pointed gourd. DOI: 10.3329/bjar.v35i1.5874Bangladesh J. Agril. Res. 35(1) : 135-142, March 2010

Direct plant regeneration from mature leaf explants of pistachio, Pistacia vera L.

2009 ◽  
Vol 121 (3) ◽  
pp. 361-365 ◽  
Author(s):  
Engin Tilkat ◽  
Ahmet Onay ◽  
Hakan Yıldırım ◽  
Emine Ayaz
Keyword(s):  
Plant Regeneration ◽  
Leaf Explants ◽  
Mature Leaf ◽  
Pistacia Vera ◽  
Direct Plant Regeneration

In Vitro direct plant regeneration using shoot tip explants in sugarcane (Saccharum officinarum L.) for rapid mass cloning

2017 ◽  
Vol 37 (2) ◽  
Author(s):  
Rupinder Kaur ◽  
Manish Kapoor
Keyword(s):  
Plant Regeneration ◽  
Saccharum Officinarum ◽  
Shoot Tip ◽  
Direct Plant Regeneration ◽  
Shoot Tip Explants ◽  
Rapid Mass

In Vitro direct plant regeneration using shoot tip explants in sugarcane (Saccharum officinarum L.) for rapid mass cloning

scholarly journals A Protocol for In-vitro direct plant regeneration from leaf tissues for micropropagation of sugarcane

2017 ◽  
Vol 29 (1) ◽  
pp. 55
Author(s):  
D. N. Balagalla ◽  
A. Wijesuriya ◽  
N. P. Ranathunge ◽  
A. M. M. S. Perera
Keyword(s):  
Plant Regeneration ◽  
Leaf Tissues ◽  
Direct Plant Regeneration

scholarly journals ISOLATION AND CULTURE OF Celosia cristata L. CELL SUSPENSION PROTOPLASTS

2003 ◽  
Vol 9 (1) ◽  
pp. 1-6
Author(s):  
Retno Mastuti ◽  
Hiroshi Miyake ◽  
Takeshi Taniguchi ◽  
Yoji Takeoka
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
Protoplast Culture ◽  
Somatic Hybridization ◽  
Culture Media ◽  
Developmental Competence ◽  
Gelling Agent ◽  
Ms Medium ◽  
L Cell ◽  
Celosia Cristata

Developmental competence of Celosia cristata L. cell suspension-derived protoplasts was investigated. The protoplasts were isolated from 3- to 9-d old cultures in enzyme solution containing 2 percent (w/v) Cellulase YC and 0.5 percent (w/v) Macerozyme R-10 which was dissolved in washing solution (0.4 M mannitol and 10 mM CaCl2) at pH 5.6 for 3 hours. The highest number of viable protoplasts was released from 5-d old culture of a homogenous cell suspension. Subsequently, three kinds of protoplast culture media were simultaneously examined with four kinds of concentration of gelling agent. Culturing the protoplasts on KM8p medium solidified with 1.2 percent agarose significantly enhanced plating efficiency as well as microcolony formation. Afterwards, the microcalli actively proliferated into friable watery callus when they were subcultured on MS medium supplemented with 0.3 mg/l 2,4-D and 1.0 mg/l kinetin. Although the plant regeneration from the protoplasts-derived calli has not yet been obtained, the reproducible developmental step from protoplasts to callus in this study may facilitate the establishment of somatic hybridization using C. cristata as one parent.

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