Isolation and Identification of Klebsiella Species from Various Clinical Samples at a Tertiary Care Hospital, South India

2019 ◽  
Vol 10 (11) ◽  
pp. 4033
Author(s):  
K. Sathyavathy ◽  
B. Kiran Madhusudhan
Keyword(s):  
South India ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Clinical Samples ◽  
Care Hospital ◽  
Isolation And Identification ◽  
Klebsiella Species

scholarly journals Prevalence and antibiotic susceptibility pattern of Klebsiella species isolated from various clinical samples in a tertiary care hospital Coimbatore

2021 ◽  
Vol 8 (10) ◽  
pp. 4886
Author(s):  
Vijayashree V. ◽  
Saikeerthana D. ◽  
Prabha P.
Keyword(s):  
Antibiotic Susceptibility ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Antibiotic Sensitivity ◽  
Clinical Samples ◽  
Care Hospital ◽  
Susceptibility Pattern ◽  
Klebsiella Species ◽  
Antibiotic Susceptibility Pattern ◽  
Sensitivity Pattern

Background: The genus Klebsiella of Enterobacteriaceae family is ubiquitous in nature. They cause many nosocomial infections like pneumonia, urinary tract infections, wound infections, bacteremia and septicemia. Multidrug resistance is seen in Klebsiella which serves as the most common cause of increased morbidity and mortality. This study reveals the prevalence and antibiotic sensitivity pattern of Klebsiella species from various clinical samples. The primary objectives are as follows: To isolate and characterize Klebsiella species from various clinical samples. To study the antibiotic susceptibility pattern of Klebsiella isolates.Methods: This prospective study was conducted in our tertiary care hospital during the period from August 2019 to October 2019. A total of 3521 samples were tested during this period. The samples include blood, sputum, urine, and pus.Results: Out of the total samples tested,1106 samples were showing the growth of the organisms. Among this,351 were identified as Klebsiella species and the highest rate of isolation of Klebsiella species is from the sputum sample and also the same was reported high in medical wards. The study also shows that the isolation of Klebsiella species shows male preponderance when compared to females. The antibiotic sensitivity pattern was done by Kirby-Bauer's disc diffusion method and the sensitivity was noted to be higher to amikacin and ciprofloxacin.Conclusions: Thus, this study shows the prevalence rate of Klebsiella species and sensitivity pattern of Klebsiella, which may help select appropriate antibiotics and prevent overuse and misuse of antibiotics.

scholarly journals Prevalence of nonfermentative gram-negative bacilli and their antimicrobial susceptibility profiles in a tertiary care hospital of Eastern India

2018 ◽  
Vol 5 (2) ◽  
pp. 366 ◽  
Author(s):  
Mandira Sarkar ◽  
Jagadananda Jena ◽  
Dipti Pattnaik ◽  
Bandana Mallick
Keyword(s):  
Antimicrobial Susceptibility ◽  
Nosocomial Infections ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Burkholderia Cepacia Complex ◽  
Diffusion Method ◽  
Clinical Samples ◽  
Care Hospital ◽  
Isolation And Identification ◽  
Gram Negative

Background: Nonfermentative gram-negative bacilli (nonfermenters) have emerged as a major concern for nosocomial infections. They exhibit resistance not only to the beta-lactam and other group of antibiotics but also to carbapenems. This study was undertaken to know the prevalence of nonfermenters from clinical samples along with their antimicrobial susceptibility profile.Methods: A cross-sectional study over a period of 21 months in the microbiology laboratory of a tertiary care hospital was done. Clinical samples were processed by conventional bacteriological methods for isolation and identification. Susceptibility testing was done by Kirby-Bauer disc diffusion method as recommended by Clinical and Laboratory Standard Institute.Results: 411 nonfermenters (13.18%) were isolated from 3116 culture positive clinical samples. Out of these nonfermenters, most were Acinetobacter baumannii (51.34%) followed by Pseudomonas aeruginosa (42.09%), Burkholderia cepacia complex (4.38%) and others (2.19%). Others included Burkholderia pseudomellei, Acinetobacter lwoffii and Stenotrophomonas maltophilia. Highest sensitivity to gentamicin and amikacin were shown by A. baumannii and P. aeruginosa respectively while both were mostly resistant to ceftriaxone. Burkholderia and Stenotrophomonas species showed 100% sensitivity to cotrimoxazole. A. baumannii was the most prevalent nonfermenter in intensive care units.Conclusions: Timely identification of nonfermenters and monitoring their susceptibility patterns will help in proper management of infections caused by them. Improved antibiotic stewardship and infection control measures should be implemented to prevent nosocomial infections and spread of drug resistant nonfermenters.

scholarly journals MULTI-DRUG RESISTANT ACINETOBACTER SPECIES FROM VARIOUS CLINICAL SAMPLES IN A TERTIARY CARE HOSPITAL FROM SOUTH INDIA

2013 ◽  
Vol 06 (12) ◽  
Author(s):  
Vijayan Sivaranjani ◽  
Sivaraman Umadevi ◽  
Sreenivasan Srirangaraj ◽  
Arunava Kali ◽  
Kunigal S Seetha
Keyword(s):  
South India ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Clinical Samples ◽  
Care Hospital ◽  
Drug Resistant ◽  
Acinetobacter Species

scholarly journals Frequency of MexA gene in pseudomonas aeruginosa isolated from clinical samples of a Tertiary Care Hospital in Pakistan.

2020 ◽  
Vol 27 (11) ◽  
pp. 2389-2393
Author(s):  
Syed Luqman Shuaib ◽  
Amina Gul ◽  
Jawad Ahmed ◽  
Noor Rehman ◽  
Liaqt Ali ◽  
...  
Keyword(s):  
Pseudomonas Aeruginosa ◽  
Resistance Genes ◽  
Efflux Pump ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Clinical Samples ◽  
Specific Gene ◽  
Care Hospital ◽  
Conventional Pcr ◽  
Isolation And Identification

The drug resistance genes are responsible to preserve the Pseudomonas. This also happens in the case of Mex drug efflux pumps and expression increase of MexA, MexB and OprM genes in Pseudomonas aeruginosa, when grown in sub-inhibitory concentrations of antibiotics. Objectives: This study was designed to detect the MexA gene of Pseudomonas aeruginosa resistant strains in tertiary care hospital, Peshawar. Study Design: Cross-sectional study. Setting: Department of Pathology, Khyber Teaching Hospital, Peshawar, Pakistan. Period: 14 months duration from April 2015 to May 2016. Material & Methods: The specimens including burn wound swabs, pus and urine were obtained from different patients and were processed on blood agar and MacConkey medium for isolation and identification. Conventional PCR was performed for the MexA gene on 50 specimens. Results: The simple conventional PCR was done for MexA (The Mex AB OprM operon resistance genes) and the O-antigen acetylase gene (the species-specific gene) separately, gave positive bands for 49 out of the 50 specimens. Our finding confirms the presence of the MexA gene (and hence most probably MexABOprM operon) in 49 out of 50 specimens of Pseudomonas aeruginosa. Conclusion: Among other resistance mechanisms to antibiotics and disinfectants, the MexABOprM efflux pump might have a role.

scholarly journals Susceptibility Pattern of Fosfomycin Against Drug Resistant Bacteria Obtained from Non - Urinary Clinical Samples in a Tertiary Care Hospital, South India

2018 ◽  
Vol 5 (5) ◽  
pp. A367-372
Author(s):  
Premalatha Ethirajulau ◽  
Jeyakumari Duraipandian ◽  
Kandasamy Sankararaman ◽  
Sony Mary Paul ◽  
Priestly Vivekkumar ◽  
...  
Keyword(s):  
South India ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Clinical Samples ◽  
Resistant Bacteria ◽  
Care Hospital ◽  
Drug Resistant ◽  
Susceptibility Pattern ◽  
Drug Resistant Bacteria

scholarly journals Isolation, Identification, Speciation and Antibiotic Susceptibility Pattern of Klebsiella Species among Various Clinical Samples at Tertiary Care Hospital

2021 ◽  
pp. 78-87
Author(s):  
K. Sathyavathy ◽  
B. Kiran Madhusudhan
Keyword(s):  
Antimicrobial Susceptibility ◽  
Tertiary Care Hospital ◽  
Tertiary Care ◽  
Clinical Samples ◽  
Care Hospital ◽  
Susceptibility Pattern ◽  
Klebsiella Species ◽  
Antimicrobial Susceptibility Pattern ◽  
Strip Method ◽  
Phenotypic Methods

To isolate, identify and speciate Klebsiella from various clinical samples and their Antimicrobial susceptibility pattern at tertiary care hospital, Chennai. Out of this 76 ESBL producing Klebsiella pneumoniae, 64(84%) were positive by Double Disc Synergy Test (DDST), 71(93%) by phenotypic confirmatory disc diffusion test (PCDDT) and 69(90.7%) by Etest strip method. Sensitivity was found to be maximum (93%) by PCDDT method, compared to other phenotypic methods such as DDST and Etest strip method. The Present study also highlights the need for the continued monitoring of Antimicrobial susceptibility patterns of important bacterial pathogens, so that rational antibiotic policies can be formulated.

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