The Polymorphism of EME1 Gene is Associated with an Increased Risk of Lung Cancer: A Case-Control Study from Chinese Population

Interstitial lung disease (ILD) seems to be associated with an increased risk of lung cancer (LC) and to have a poorer prognosis than LC without ILD. The frequency of ILD in an LC cohort and its prognosis implication need to be better elucidated. This retrospective, observational, cohort study evaluated the frequency of ILD among LC patients (LC–ILD) diagnosed over a 2-year period. LC–ILD patients’ characteristics were compared to those with LC without ILD (LC–noILD). Lastly, we conducted a case–control study within this cohort, matching three LC–noILDs to each LC–ILD patient, to evaluate the ILD impact on LC patients’ prognoses. Among 906 LC patients, 49 (5.4%) also had ILD. Comparing LC–ILD to LC–noILD patients, respectively, more were men (85.7% vs. 66.2%; p = 0.02); adenocarcinomas were less frequent (47.1% vs. 58.7%, p = 0.08); median [range] and overall survival was shorter: (9 [range: 0.1–39.4] vs. 17.5 [range: 0.8–50.4] months; p = 0.01). Multivariate analysis (hazard ratio [95% confidence interval]) retained two factors independently associated with LC risk of death: ILD (1.79 [1.22–2.62]; p = 0.003) and standard-of-care management (0.49 [0.33–0.72]; p < 0.001). Approximately 5% of patients with a new LC diagnosis had associated ILD. ILD was a major prognosis factor for LC and should be taken into consideration for LC management. Further studies are needed to determine the best therapeutic strategy for the LC–ILD population.

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Association between polymorphisms in the GSTA4 gene and risk of lung cancer: A case-control study in a Southeastern Chinese population

Molecular Carcinogenesis ◽

10.1002/mc.20478 ◽

2008 ◽

Vol 48 (3) ◽

pp. 253-259 ◽

Cited By ~ 6

Author(s):

Ji Qian ◽

Jianying Jing ◽

Guangfu Jin ◽

Haifeng Wang ◽

Yi Wang ◽

...

Keyword(s):

Lung Cancer ◽

Chinese Population ◽

Case Control Study ◽

Case Control ◽

Control Study

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Alpha-1 Antitrypsin Deficiency and Risk of Lung Cancer in Never-Smokers: A Multicentre Case-Control Study

10.21203/rs.3.rs-1032507/v1 ◽

2021 ◽

Author(s):

Ramón Antonio Tubío-Pérez ◽

María Torres-Durán ◽

María Esmeralda García-Rodríguez ◽

Cristina Candal-Pereira ◽

Julia Rey-Brandariz ◽

...

Keyword(s):

Lung Cancer ◽

Case Control Study ◽

Case Control ◽

Increased Risk ◽

Antitrypsin Deficiency ◽

Alpha 1 Antitrypsin Deficiency ◽

Alpha 1 Antitrypsin ◽

Never Smokers ◽

Epidemiological Characteristics ◽

Control Study

Abstract Background Lung cancer (LC) is the most commonly diagnosed cancer and the leading cause of cancer-related death in both sexes worldwide. Although its principal risk factor is smoking habit, there are genetic mutations, such as alpha-1 antitrypsin deficiency (AATD), that have been related with increased risk This study is the continuation of an earlier one published by the same group in 2015, aimed at analysing risk of LC in never-smokers, associated with carriers of the AATD genotype. Methods A multicentre case-control study was conducted in Spain across the period January 2011 to August 2019. Cases were patients with LC, and controls were patients, all never-smokers, undergoing major non-cancer-related surgery. Data were collected on epidemiological characteristics, exposure to environmental tobacco smoke (ETS), residential radon levels, and alpha-1 antitrypsin (AAT) genotype. Results The study included 457 cases (42%) and 631 controls (58%), with a predominance of women. The most frequent histological type was adenocarcinoma (77.5%), followed by squamous cell carcinoma (7.7%). No association of risk of LC was found with the status of AATD genotype carrier, both overall and broken down by age, sex, or exposure to ETS. Conclusions No risk association was found between being a carrier of an AAT deficiency genotype and LC among never-smokers. Even so, new studies are required to provide fuller information in this regard with respect to never-smokers, and possibly even include previous respiratory diseases.

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Is Opium Use Associated with an Increased Risk of Lung Cancer? A Case-Control Study

10.21203/rs.2.15585/v3 ◽

2020 ◽

Author(s):

Ahmad Naghibzadeh Tahami ◽

Maryam Marzban ◽

Vahid Yazdi Feyzabadi ◽

Shahryar Dabiri ◽

Shokrollah Mohseni ◽

...

Keyword(s):

Lung Cancer ◽

Case Control Study ◽

Conditional Logistic Regression ◽

Daily Intake ◽

Case Control ◽

Control Group ◽

Related Risk ◽

Increased Risk ◽

Control Study ◽

Opium Use

Abstract Background: In recent years, lung cancer (LC) incidence has increased in Iran. The use of opium and its derivatives (O&D) has increased as well. This study aimed to investigate the association between the use of O&D and LC incidence.Methods: In this case-control study conducted in Kerman, Iran; 140 patients with lung cancer and 280 healthy controls matched by age, sex, and place of residence were included. Data, including O&D use, cigarette smoking, alcohol use, and diet, were collected using a structured questionnaire. The relation between the use of O&D and LC was evaluated using conditional logistic regression adjusted for tobacco smoking, education, daily intake of fruit, vegetables, red meat, and hydrogenated fats.Results: Opium ever-use was associated with an increased risk of LC (Adjusted Odds Ratio (AOR) =5.95, 95 % CI: 1.87 -18.92). Participants were divided into low and high use groups based on the median of opium use in the control group. A significant dose-response relation was observed between the amount of daily O&D use and LC; and the relation was stronger in high users (AOR low users = 3.81 % CI: 1.13 -12.77 and OR high users= 9.36, 95% CI: 2.05 -42.72). Also, LC was higher among participants starting the use of O&D at younger ages (≤ 41 years old vs never users AOR = 8.64, 95 % CI: 1.90 -39.18) compared to those who started at an older age ( >41 years old vs never users, AOR = 4.71, 95 % CI: 1.38 - 16.08). The association between opium, and lung cancer among non-smokers was OR: 6.50 (95% CI: 2.89 to 14.64).Conclusion: The results of this study show that opium use is probably a dose related risk factor for lung cancer.

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Is Opium Use Associated with an Increased Risk of Lung Cancer? A Case-Control Study

10.21203/rs.2.14374/v1 ◽

2019 ◽

Author(s):

Ahmad Naghibzadeh-Tahami ◽

Maryam Marzban ◽

Vahid Yazdi-Feyzabadi ◽

Shahryar Dabiri ◽

Reza Abbasi Rayeni ◽

...

Keyword(s):

Lung Cancer ◽

Case Control Study ◽

Conditional Logistic Regression ◽

Case Control ◽

Test Results ◽

Increased Risk ◽

Study Support ◽

Control Study ◽

Opium Use ◽

Structured Questionnaire

Abstract Background: In recent years, lung cancer (LC) incidence has increased in Iran. The use of opium and its derivatives (O&D) has increased as well. The aim of this study was to investigate the association between the use of O&D and LC incidence. Methods: In this case-control study conducted in Kerman, Iran; 140 patients with lung cancer and 284 healthy controls matched by age, sex, and place of residence were included. Data including O&D use, cigarette smoking, alcohol use, and diet were collected using a structured questionnaire. The relation between the use of O&D and LC was evaluated using conditional logistic regression test. Results: The use of opium was associated with an increased risk of LC (Adjusted Odds Ratio (AOR) = 7.95, 95% CI: 3.78 -16.73). A significant dose-response relation between the use of opium and its derivatives was observed (low use AOR = 7.47, 95% CI: 2.33-23.98 and high use AOR = 11.57, 95% CI: 2.25-59.49). Also, there was a relation between starting to use O&D at lower ages and an increased risk of LC (AOR = 5.78, 95 % CI: 2.36 - 14.14). Conclusion: The results of this study support that opium use could be considered as a strong risk factor for LC. Thus, it is highly suggested that tailored policies to be applied in order to reduce use of opium.

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Combined and interaction effect of Chlamydia pneumoniae infection and smoking on lung cancer: a case-control study in southeast China

10.21203/rs.2.23930/v2 ◽

2020 ◽

Author(s):

Xin Xu ◽

Zhiqiang Liu ◽

Weimin Xiong ◽

Minglian Qiu ◽

Shuling Kang ◽

...

Keyword(s):

Lung Cancer ◽

Passive Smoking ◽

Chlamydia Pneumoniae ◽

Case Control Study ◽

Case Control ◽

Combined Effects ◽

Increased Risk ◽

History Of ◽

Control Study ◽

History Of Cancer

Abstract Background: This case-control study investigated the role of Chlamydia pneumoniae (Cpn) infection in the pathogenesis of lung cancer and the combined and interaction effect of Cpn infection, smoking, and various environmental factors.Methods: The study comprised 449 lung cancer patients and 512 age- and gender-matched healthy controls. All participants provided a 5 ml fasting peripheral venous blood sample for testing Cpn-specific IgG and IgA by using micro-immunofluorescence. Besides analyzing the associations between Cpn and lung cancer, combined effect analysis, logistic regression, and the Excel table made by Andersson were used to analyze the combined and interaction effects of Cpn and environmental factors on lung cancer.Results: Compared to those with no evidence of serum Cpn IgA or Cpn IgG, those with both Cpn IgG+ and IgA+ had 2.00 times the risk (95% CI: 1.34–3.00) of developing lung cancer. Cpn IgG+ or IgA+ was associated with a significantly increased risk of lung cancer among smokers; the adjusted odds ratio (OR) was 1.79 (95% CI: 1.10–2.91) and 2.27 (95% CI: 1.38–3.72), respectively. Those exposed to passive smoking with Cpn IgG+ or IgA+ also showed an increased risk of lung cancer; the adjusted OR was 1.82 (95% CI: 1.20–2.77) or 1.87 (95% CI: 1.22–2.87), respectively. Similar results were also observed among alcohol drinkers. Multiplicative and additive interactions were not observed between Cpn infection and environmental factors. The combined effects of Cpn IgG+ or IgA+ with smoking, passive smoking, and family history of cancer on lung cancer were determined.Conclusion: Cpn infection is potentially associated with primary lung cancer in the Chinese Han population and has combined effects with smoking, passive smoking, and family history of cancer.

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A Functional Polymorphism in Accessible Chromatin Region Confers Risk of Non-Small Cell Lung Cancer in Chinese Population

Frontiers in Oncology ◽

10.3389/fonc.2021.698993 ◽

2021 ◽

Vol 11 ◽

Author(s):

Jieyi Long ◽

Tingting Long ◽

Ying Li ◽

Peihong Yuan ◽

Ke Liu ◽

...

Keyword(s):

Lung Cancer ◽

Small Cell Lung Cancer ◽

Chinese Population ◽

Case Control Study ◽

Case Control ◽

Small Cell ◽

Small Cell Lung ◽

Using Data ◽

Accessible Chromatin ◽

Control Study

BackgroundThe disease-associated non-coding variants identified by genome-wide association studies (GWASs) were enriched in open chromatin regions (OCRs) and implicated in gene regulation. Genetic variants in OCRs thus may exert regulatory functions and contribute to non-small cell lung cancer (NSCLC) susceptibility.ObjectiveTo fine map potential functional variants in GWAS loci that contribute to NSCLC predisposition using chromatin accessibility and histone modification data and explore their functions by population study and biochemical experimental analyses.MethodsWe mapped the chromatin accessible regions of lung tissues using data of assay for transposase-accessible chromatin using sequencing (ATAC-seq) in The Cancer Genome Atlas (TCGA) and prioritized potential regulatory variants within lung cancer GWAS loci by aligning with histone signatures using data of chromatin immunoprecipitation assays followed by sequencing (ChIP-seq) in the Encyclopedia of DNA Elements (ENCODE). A two-stage case–control study with 1,830 cases and 2,001 controls was conducted to explore the associations between candidate variants and NSCLC risk in Chinese population. Bioinformatic annotations and biochemical experiments were performed to further reveal the potential functions of significant variants.ResultsSixteen potential functional single-nucleotide polymorphisms (SNPs) were selected as candidates from bioinformatics analyses. Three variants out of the 16 candidate SNPs survived after genotyping in stage 1 case–control study, and only the results of SNP rs13064999 were successfully validated in the analyses of stage 2 case–control study. In combined analyses, rs13064999 was significantly associated with NSCLC risk [additive model; odds ratio (OR) = 1.17; 95%CI, 1.07–1.29; p = 0.001]. Functional annotations indicated its potential enhancer bioactivity, and dual-luciferase reporter assays revealed a significant increase in luciferase activity for the reconstructed plasmid with rs13064999 A allele, when compared to the one with wild-type G allele (pA549 < 0.001, pSK-MES-1 = 0.004). Further electrophoretic mobility shift assays (EMSA) and super-shift assays confirmed a stronger affinity of HP1γ for the binding motif containing SNP rs13064999 A allele.ConclusionThese findings suggested that the functional variant rs13064999, identified by the integration of ATAC-seq and ChIP-seq data, contributes to the susceptibility of NSCLC by affecting HP1γ binding, while the exact biological mechanism awaits further exploration.

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Combined and interaction effect of chlamydia pneumoniae infection and smoking on lung cancer: a case-control study in Southeast China

BMC Cancer ◽

10.1186/s12885-020-07418-8 ◽

2020 ◽

Vol 20 (1) ◽

Author(s):

Xin Xu ◽

Zhiqiang Liu ◽

Weimin Xiong ◽

Minglian Qiu ◽

Shuling Kang ◽

...

Keyword(s):

Lung Cancer ◽

Environmental Factors ◽

Passive Smoking ◽

Chlamydia Pneumoniae ◽

Case Control Study ◽

Case Control ◽

Increased Risk ◽

History Of ◽

Control Study ◽

History Of Cancer

Abstract Background This case-control study investigated the role of Chlamydia pneumoniae (Cpn) infection in the pathogenesis of lung cancer and the combined and interaction effect of Cpn infection, smoking, and various environmental factors. Methods The study comprised 449 lung cancer patients and 512 age- and sex-matched healthy controls. All participants provided a 5 ml fasting peripheral venous blood sample for testing Cpn-specific IgG and IgA by using micro-immunofluorescence. Besides analyzing the associations between Cpn and lung cancer, combined effect analysis, logistic regression, and the Excel table made by Andersson were used to analyze the combined and interaction effects of Cpn and environmental factors on lung cancer. Results Compared to those with no evidence of serum Cpn IgA or Cpn IgG, those with both Cpn IgG+ and IgA+ had 2.00 times the risk (95% CI: 1.34–3.00) of developing lung cancer. Cpn IgG+ or IgA+ was associated with a significantly increased risk of lung cancer among smokers; the adjusted odds ratio (OR) was 1.79 (95% CI: 1.10–2.91) and 2.27 (95% CI: 1.38–3.72), respectively. Those exposed to passive smoking with Cpn IgG+ or IgA+ also showed an increased risk of lung cancer; the adjusted OR was 1.82 (95% CI: 1.20–2.77) or 1.87 (95% CI: 1.22–2.87), respectively. Similar results were also observed among alcohol drinkers. Multiplicative and additive interactions were not observed between Cpn infection and environmental factors. The combined effects of Cpn IgG+ or IgA+ with smoking, passive smoking, and family history of cancer on lung cancer were determined. Conclusion Cpn infection is potentially associated with primary lung cancer in the Chinese Han population and has combined effects with smoking, passive smoking, and family history of cancer.

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