Genetic and morphological diversity in Stachys lavandulifolia (Lamiaceae) populations

Biologija ◽  
2016 ◽  
Vol 62 (1) ◽  
Author(s):  
Masoud Sheidai ◽  
Fatemeh Taban ◽  
Seyed Mehdi Talebi ◽  
Zahra Noormohammadi
Keyword(s):  
Genetic Diversity ◽  
Medicinal Plant ◽  
Gene Diversity ◽  
Genetic Difference ◽  
Morphological Variability ◽  
Population Diversity ◽  
Morphological Data ◽  
Gene Pools ◽  
Germplasm Management ◽  
Geographical Populations

Stachys lavandulifolia Vahl. (Lamiaceae) is an important medicinal plant that grows in different parts of Iran and forms many geographical populations. We have no information on its population genetic structure, genetic diversity, and morphological variability in Iran. Therefore, we planned a genetic and morphological investigation in St. lavandulifolia geographical populations in Iran. The obtained data are important for conservation and germplasm management of this medicinal plant species. Seventy-four plants were randomly collected from 14 geographical populations and studied for genetic diversity (ISSR molecular markers) and morphological variability. The  highest value for gene diversity occurred in populations 1 and 4 (0.133 and 0.129, respectively). The  latitude and altitude were positively correlated with gene diversity and genetic polymorphism while longitude was negatively correlated with them. The Mantel test showed correlation between genetic distance and geographical distance. AMOVA revealed a significant genetic difference among populations and showed that 58% of total genetic variation was due to within-population diversity. The STRUCTURE analysis and K-Means clustering identified two gene pools for St. lavandulifolia. The  consensus tree of both molecular and morphological data identified divergent populations.

scholarly journals Genetic diversity analysis of the medicinal plant Heracleum persicum desf. ex fischer

Genetika ◽  
2021 ◽  
Vol 53 (2) ◽  
pp. 757-768
Author(s):  
Faegheh Behzadifar ◽  
Sayeh Marandi ◽  
Ahmad Majd ◽  
Fahimeh Salimpour ◽  
Sedigheh Arbabian
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Genetic Variability ◽  
Medicinal Plant ◽  
Genetic Conservation ◽  
Gene Pools ◽  
Breeding Programs ◽  
Good Level ◽  
Heracleum Persicum ◽  
Geographical Populations

Persian hogweed (Heracleum persicum Desf. ex Fischer) is native to Iran but was introduced to Europe as an invasive plant. It has medicinal and edible values. Its fruits have been used to relieve flatulence, stomach aches as well as a flavoring as a digestive and an antiseptic. This medicinal plant forms many geographical populations in the country, particularly in mountainous regions; however, we have no data on the genetic structure and genetic diversity of this plant species. Such information can be used in gene pool identification and future genetic conservation and breeding programs. Therefore, the present study was performed with the following aims: 1- Provide data on genetic diversity of geographical populations, 2- Identify the potential gene pools for future genetic conservation and breeding programs. We used both Inter-simple sequence repeats (ISSR) and the Start Codon Targeted (SCoT) molecular markers as these are suitable molecular markers for genetic diversity investigations. There are highly polymorphic, reproducible, and relatively low cost markers. The present study revealed that both ISSR and SCoT markers produce data on the genetic variability and genetic affinity of the local populations. Both molecular markers revealed a good level of genetic variability within and among Heracleum persicum populations. Analysis of molecular variance (AMOVA) produced a significant differences between geographical populations for both markers. We could identify few ISSR as well as SCoT bands which can differentiate the studied populations. The moderate to a good level of genetic diversity we observed within each H. persicum may be due to cross pollination. In conclusion, we suggest using combination of ISSR and SCoT molecular markers to study population genetic variability in H. persicum geographical populations for future genetic conservation and germplasm collection of this medicinal plant.

scholarly journals Genetic and morphological variability in medicinal plant Helichrysum oocephalum Boiss. (Asteraceae) in Iran

Hacquetia ◽  
2020 ◽  
Vol 19 (2) ◽  
pp. 317-324
Author(s):  
Mobina Abbaszadeh ◽  
Masoud Sheidai ◽  
Narges Azizi ◽  
Fahimeh Koohdar
Keyword(s):  
Medicinal Plant ◽  
Population Genetic ◽  
Morphological Analysis ◽  
Morphological Variability ◽  
Gene Pools ◽  
Genetic Investigation ◽  
Morphological Studies ◽  
Geographical Populations ◽  
The Mean ◽  
Issr Molecular Markers

AbstractHelichrysum oocephalum is a medicinal plant of the genus Helichrysum that have limited distribution in Iran. Local geographical populations may differ in their genetic content and form different gene pools. Therefore, we carried out population genetic investigation and morphological studies in five geographical populations of Helichrysum oocephalum by using ISSR molecular markers. AMOVA produced the significant genetic differences. The mean Nm value revealed some degree of gene flow among Helichrysum oocephalum 8. Molecular and morphological analysis indicated that we have 2 groups in the studied populations. The present findings may be of use in the conservation of this medicinal plant in Iran.

scholarly journals Population genetic diversity and structure in Ziziphora tenuior L.: Identification of potential gene pools

Genetika ◽  
2016 ◽  
Vol 48 (2) ◽  
pp. 565-578
Author(s):  
Raheleh Tabaripoor ◽  
Masoud Sheidai ◽  
Seyyed Talebi ◽  
Zahra Noormohammadi
Keyword(s):  
Genetic Diversity ◽  
Population Genetic ◽  
Isolation By Distance ◽  
Genetic Difference ◽  
Gene Pools ◽  
Diversity Analysis ◽  
Genetic Diversity And Structure ◽  
Main Gene ◽  
Geographical Populations ◽  
Issr Molecular Markers

Ziziphora tenuior L. is a medicinal plant species of the genus Ziziphora (Labiatae) that grows in different areas of Iran. In order to study the population genetic structure in Ziziphora tenuior, we collected 107 plant specimens from 20 geographical populations that are located in 17 provinces. ISSR molecular markers were used for genetic diversity analysis. The populations studied revealed intra- and inter-population genetic variability. AMOVA test showed significant genetic difference among the studied populations. STRUCTURE plot identified two main gene pools for Ziziphora tenuior in Iran. These populations showed isolation by distance and restrict gene flow occurred among them.

Species delimitation and population structure in three Onosma (Boraginaceae) species

Biologia ◽  
2015 ◽  
Vol 70 (10) ◽  
Author(s):  
Masoud Sheidai ◽  
Samira Sadeghi ◽  
Mahnaz-Arab Ameri ◽  
Ahmad-Reza Mehrabeian
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Species Delimitation ◽  
Genetic Difference ◽  
Morphological Variability ◽  
Morphological Diversity ◽  
Mantel Test ◽  
Geographical Distance ◽  
Nj Tree ◽  
Issr Molecular Markers

AbstractThe present study aimed to reveal genetic diversity and population structure in three Onosma species i.e., Onosma bulbotrichum DC., O. elwendicum Wettst. and O. sericeum Willd. (Boraginaceae) growing in Iran. This study was performed to check if the ISSR molecular markers could be used in the Onosma species delimitation. We also investigated the infra-specific morphological variability and if we can identify infra-species taxonomic forms. The present study revealed within species genetic and morphological diversity. ISSR data could delimit the studied species as they were separated from each other in NJ tree, maximum parsimony and STRUCTURE analysis. AMOVA and Hickory tests produced significant genetic difference among the studied species. The Mantel test showed no correlation between genetic distance and geographical distance of the populations for all three species. Reticulation analysis and Nm estimation showed gene flow among these populations. We could identify a few ISSR loci that are adaptive. Data obtained can be used in conservation of these medicinal plants in Iran.

scholarly journals Assessment of Genetic Diversity of Promising Castor Been (Ricinus communis L.) Genotypes in Nigeria

2019 ◽  
Vol 11 (3) ◽  
pp. 467-474
Author(s):  
Bolaji Zuluqurineen SALIHU ◽  
Olamide Ahmed FALUSI ◽  
Adeyinka Olufemi ADEPOJU ◽  
Ibrahim Wasiu AROLU ◽  
Oladipupo Yusuf DAUDU ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Diversity Index ◽  
Gene Diversity ◽  
Ricinus Communis ◽  
Polymorphic Information Content ◽  
Block Design ◽  
Genetic Material ◽  
Morphological Data ◽  
Research Attention ◽  
Ricinus Communis L

Castor oil plant (Ricinus communis L.) is an important oil crop with little research attention in Nigeria. In the present research, extent of genetic diversity among 20 Nigerian castor genotypes was determined using morphological descriptors and molecular markers. The genotypes were laid out on a randomized complete block design with three replicated plots. Molecular genotyping of the genotypes was carried out using genomic Simple Sequence Repeats (SSR). The genotypes revealed high divergence in seed colour, seed shape, seed mottle, seed caruncle and seed sizes. Seedling establishment varied from 70.18% (in Acc. 006) to 93.25% (Acc. 001) with average mean of 81.53%. Raceme length ranged from 15.90 cm to 29.54 cm with population mean of 20.80 cm. The highest seed yield (1222.98 kg/ha) was recorded in Acc. 001 and the least (611.46 kg/ha) was observed in Acc. 006. Seed oil content varied between 32.15% in Acc. 042 and 54.03% in Acc. 006. Agglomerative cluster dendrogram constructed from morphological data showed random distribution of the genotypes into three cluster groups irrespective of the sources/collection points. The genetic diversity based on SSR Marker Analysis revealed high average expected heterozygosity (0.74), Polymorphic information content (0.68), Nei’s gene diversity index (0.72) and Shannon's Information index (1.43). The dendrogram constructed from molecular data grouped the twenty genotypes into three groups at coefficient of 0.34. From these findings, it showed that the twenty genotypes evaluated are divergent in nature and they could serve as good genetic material for castor breeding in Nigeria.

Genetic diversity of eight millet genera assessed by using molecular and morphological markers

2016 ◽  
Vol 67 (2) ◽  
pp. 181
Author(s):  
Zdislava Dvořáková ◽  
Petra Hlásná Čepková ◽  
Iva Viehmannová ◽  
Lenka Havlíčková ◽  
Dagmar Janovská
Keyword(s):  
Genetic Diversity ◽  
Morphological Variability ◽  
Aflp Markers ◽  
Morphological Data ◽  
Plant Materials ◽  
Morphological Attributes ◽  
Shannon’S Index ◽  
High Level ◽  
Close Relatedness ◽  
Nei's Genetic Distance

In this study, the genetic diversity and relationships among eight millet genera were investigated by molecular and morphological data analyses. Sixty-nine millet accessions were analysed by using amplified fragment length polymorphism (AFLP) markers, and evaluated for morphological traits. Eight AFLP primer pairs were amplified successfully and 779 bands were scored for all accessions, with a high level of polymorphism detected. Nei’s genetic distance among all accessions varied from 0.0123 to 0.4246 and the Shannon’s index was estimated at 0.9708. The neighbour joining tree, using the unweighted neighbour-joining method and Dice’s dissimilarity coefficient, was constructed. The AFLP markers revealed the close relatedness between the Eragrostis and Panicum genera, whereas the greatest distance was found the Pennisetum and Echinochloa genera. Cluster analysis based on the AFLP profiles revealed that the majority of accessions of a given millet genus tend to group together. Clustering from morphological data allocated individuals into three main clusters with high variation. The genetic variability found between the analysed accessions was weakly negatively correlated (r = –0.074) with their morphological attributes. However, high molecular and morphological variability indicated that this collection includes rich and valuable plant materials for millet breeding.

scholarly journals Understanding population differentiation using geographical, morphological and genetic characterization in Erodium cicunium

2020 ◽  
Vol 80 (04) ◽  
Author(s):  
Xingxing Si ◽  
Lei GAO ◽  
Yuwei Song ◽  
Majid Khayatnezhad ◽  
Amir Abbas Minaeifar
Keyword(s):  
Genetic Difference ◽  
Morphological Characters ◽  
Molecular Data ◽  
Population Diversity ◽  
Start Codon ◽  
Principal Coordinates Analysis ◽  
Forage Plants ◽  
Principal Coordinates ◽  
Geographical Populations ◽  
Study Population

Erodium cicunium (Geraniaceae) species are distributed in different habitats of Iran. Some species are of medicinal importance while some are well known weeds and used as forage plants. An investigation was carried out to evaluate 124 randomly collected plants of E. cicunium from 15 geographical populations in 5 provinces to study population structure and for morphological and molecular characters. Start Codon Targeted (SCoT) markers were used to analyse molecular diversity. Analysis of molecular variance (AMOVA) revealed significant genetic difference among the studied populations and also revealed that 60% of total genetic variability was due to within population diversity, while 40% genetic differentiation was recorded among populations. Principal Coordinates Analysis (PCoA) of populations based on morphological characters was not in agreement with Metric Multidimensional Scaling (MDS) plot of molecular data.

scholarly journals Genetic Diversity and Population Structure of Endangered Catfish Rita rita (Hamilton, 1822) Revealed by Heterologous DNA Microsatellite Markers

2021 ◽  
Vol 34 (2) ◽  
Author(s):  
MUHAMMAD FORHAD ALI ◽  
◽  
MD. RAFIQUL ISLAM SARDER ◽  
MOHAMMAD MATIUR RAHMAN ◽  
MD. FAZLUL AWAL MOLLAH ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Population Structure ◽  
Gene Diversity ◽  
Polymorphic Information Content ◽  
Genetic Difference ◽  
Information Index ◽  
Hardy Weinberg Equilibrium ◽  
High Gene ◽  
High Level ◽  
Dna Microsatellite

Genetic information is essential for conservation and future aquaculture development of the endangered catfish Rita rita (Hamilton, 1822). Two hundred catfish, R. rita, 50 from four rivers, the Old Brahmaputra, Jamuna, Meghna and Kangsa were collected and analysed to evaluate the genetic diversity and population structure using five microsatellite primers (Cba06-KUL, Cba08-KUL, Cba09-KUL, Phy03-KUL and Phy07-KUL). Four of the five amplified loci were found polymorphic (P95) in all the populations and 46 alleles were recorded with 9 to 14 alleles per locus. Differences were observed in the total number of alleles ranging from 41 to 44, effective number of alleles from 29.96 to 37.46, observed heterozygosity from 0.57 to 0.76, Shannon’s information index from 2.09 to 2.30 and polymorphic information content from 0.84 to 0.88 among the four populations. Results exposed the highest levels of genetic diversity in the Meghna population while the lowest in the Kangsa population of R. rita. All the populations were significantly deviated (P < 0.001) from the Hardy-Weinberg equilibrium for all the loci. Nei’s genetic distance between populations ranged 0.007 to 0.017 with low overall genetic difference FST = 0.011 and high gene flow Nm = 24.333, indicating that R. rita populations were not subdivided. This study revealed a high level of gene diversity with deficiency in genetic heterogeneity in all the populations of R. rita, emphasising natural management, conservation and rehabilitation measures of this species.

scholarly journals Molecular Characterization of Cultivated Pawpaw (Asimina triloba) Using RAPD Markers

2003 ◽  
Vol 128 (1) ◽  
pp. 85-93 ◽  
Author(s):  
Hongwen Huang ◽  
Desmond R. Layne ◽  
Thomas L. Kubisiak
Keyword(s):  
Genetic Diversity ◽  
Gene Pool ◽  
Rapd Markers ◽  
Genetic Relatedness ◽  
Gene Diversity ◽  
Pedigree Information ◽  
Germplasm Management ◽  
Asimina Triloba ◽  
Selection History ◽  
Upgma Cluster Analysis

Thirty-four extant pawpaw [Asimina triloba (L.) Dunal] cultivars and advanced selections representing a large portion of the gene pool of cultivated pawpaws were investigated using 71 randomly amplified polymorphic DNA (RAPD) markers to establish genetic identities and evaluate genetic relatedness. All 34 cultivated pawpaws were uniquely identified by as few as 14 loci of eight primers. Genetic diversity of the existing gene pool of cultivated pawpaws, as estimated by Nei's gene diversity (He), was similar to that of wild pawpaw populations. The genetic relatedness among the cultivated pawpaws examined by UPGMA cluster analysis separated 34 cultivars and selections into two distinct clusters, a cluster of PPF (The PawPaw Foundation) selections and a cluster including a majority of the extant cultivars selected from the wild and their derived selections. The results are in general agreement with the known selection history and pedigree information available. The consensus fingerprint profile using the genetically defined RAPD markers is a useful and reliable method for establishing the genetic identities of the pawpaw cultivars and advanced selections. This also proved to be an improved discriminating tool over isozyme markers for the assessment of genetic diversity and relatedness. RAPD profiling of data presented in this study provides a useful reference for germplasm curators engaged in making decisions of sampling strategies, germplasm management and for breeders deciding which parents to select for future breeding efforts.

Genetic diversity in natural populations of Acacia mearnsii

2000 ◽  
Vol 48 (2) ◽  
pp. 279 ◽  
Author(s):  
S. D. Searle ◽  
J. C. Bell ◽  
G. F. Moran
Keyword(s):  
Genetic Diversity ◽  
Gene Diversity ◽  
New South ◽  
Natural Populations ◽  
South Australia ◽  
Population Diversity ◽  
Regional Level ◽  
Acacia Mearnsii ◽  
South Wales ◽  
Isozyme Loci

Twenty-three isozyme loci were used to examine genetic diversity within and between 19 natural populations of Acacia mearnsii De Wild. selected to represent its entire geographic range. Acacia mearnsii was found to have moderate genetic diversity (species level gene diversity HT = 0.201) with the majority (89.2%) of variation occurring within populations. All measures of population diversity were higher in the northern (New South Wales) than the southern (Victoria, South Australia, Tasmania) populations. There was some evidence of differentiation between populations but no strong clustering at a regional level.

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