Effects and mechanism of stem cells from human exfoliated deciduous teeth combined with hyperbaric oxygen therapy in type 2 diabetic rats

Abstract Objectives This study aimed to examine the impact of hyperbaric oxygen therapy (HBOT) on serum C-reactive protein (CRP) levels, osteoclast numbers, and osteoprotegerin (OPG) expression in periodontitis-induced diabetic rats Materials and Methods This study constituted an in vivo laboratory-based experiment incorporating a posttest only control group design. Thirty male Wistar rats were divided into three groups of research subjects: a healthy group (K0), periodontitis-induced diabetic group (K1), and periodontitis-induced diabetic group treated with HBOT for 7 days (K2). After treatment, the subjects were sacrificed to determine the level of serum CRP by the ELISA method. Immunohistochemical analysis was conducted to check the level of OPG expression, while a histological analysis was undertaken to quantify the number of osteoclasts. Statistical Analysis The data was analyzed using a one-way ANOVA and Least Significant Difference (LSD) test on which a result of p < 0.05 was considered statistically significant. Results HBOT appreciably decreased serum CRP levels, significantly enhancing OPG expression in periodontitis-induced diabetic (p < 0.05) and decreasing the number of osteoclasts in -periodontitis-induced diabetic (p > 0.05). Conclusion HBOT reduced the serum CRP level, increased OPG expression, and decreased osteoclast numbers in periodontitis-induced diabetic rats.

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Hyperbaric Oxygen Therapy Improves Glucose Homeostasis in Type 2 Diabetes Patients: A Likely Involvement of the Carotid Bodies

Advances in Experimental Medicine and Biology - Arterial Chemoreceptors in Physiology and Pathophysiology ◽

10.1007/978-3-319-18440-1_24 ◽

2015 ◽

pp. 221-225 ◽

Cited By ~ 14

Author(s):

P. Vera-Cruz ◽

F. Guerreiro ◽

M. J. Ribeiro ◽

M. P. Guarino ◽

S. V. Conde

Keyword(s):

Type 2 Diabetes ◽

Hyperbaric Oxygen ◽

Glucose Homeostasis ◽

Hyperbaric Oxygen Therapy ◽

Oxygen Therapy ◽

Carotid Bodies ◽

Diabetes Patients

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Proliferation of neural stem cells correlates with Wnt-3 protein in hypoxic-ischemic neonate rats after hyperbaric oxygen therapy

Neuroreport ◽

10.1097/wnr.0b013e3282f0ec09 ◽

2007 ◽

Vol 18 (16) ◽

pp. 1753-1756 ◽

Cited By ~ 20

Author(s):

Xiao-Li Wang ◽

Yu-Jia Yang ◽

Min Xie ◽

Xiao-He Yu ◽

Chen-Tao Liu ◽

...

Keyword(s):

Stem Cells ◽

Neural Stem Cells ◽

Hyperbaric Oxygen ◽

Hyperbaric Oxygen Therapy ◽

Oxygen Therapy

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Role of Hyperbaric Oxygen Therapy on Microvascular Diabetic Complications and Metabolic Profile among Patients with Type 2 Diabetes Mellitus

Oman Medical Journal ◽

10.5001/omj.2020.47 ◽

2020 ◽

Vol 35 (3) ◽

pp. e129-e129

Author(s):

Abdulhakeem Al-Rawahi

Keyword(s):

Diabetes Mellitus ◽

Type 2 Diabetes ◽

Type 2 Diabetes Mellitus ◽

Hyperbaric Oxygen ◽

Diabetic Complications ◽

Hyperbaric Oxygen Therapy ◽

Metabolic Profile ◽

Oxygen Therapy

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Effect of HBO therapy on adipose-derived stem cells, fibroblasts and co-cultures: In vitro study of oxidative stress, angiogenic potential and production of pro-inflammatory growth factors in co-cultures1

Clinical Hemorheology and Microcirculation ◽

10.3233/ch-209222 ◽

2020 ◽

pp. 1-13

Author(s):

P. Engel ◽

M. Ranieri ◽

O. Felthaus ◽

S. Geis ◽

F. Haubner ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Stem Cells ◽

Wound Healing ◽

Hyperbaric Oxygen ◽

Hyperbaric Oxygen Therapy ◽

Oxygen Therapy ◽

Reactive Oxygen ◽

Adipose Derived Stem Cells ◽

Oxygen Species

BACKGROUND: A key moderator of wound healing is oxygen. Wound healing is a dynamic and carefully orchestrated process involving blood cells, cytokines, parenchymal cells (i.e. fibroblasts and mesenchymal stem cells) and extracellular matrix reorganization. Human adipose derived stem cells as well as human fibroblasts produce soluble factors, exhibit diverse effects on inflammation and anti inflammation response and are involved in wound healing processes. Hyperbaric oxygen therapy is an effective adjunct treatment for ischemic disorders such as chronic infection or chronic wounds. In vitro effects of hyperbaric oxygen therapy on human cells were presented in many studies except for those on mono- and co-cultures of human adipose derived stem cells and fibroblasts. OBJECTIVE: The aim of this study was to investigate the effects of hyperbaric oxygen therapy on mono- and co-cultures of human adipose derived stem cells and fibroblasts. METHODS: Mono- and co-cultures from human adipose derived stem cells and fibroblasts were established. These cultures were exposed to hyperbaric oxygen therapy every 24 h for five consecutive days. Measuring experiments were performed on the first, third and fifth day. Therapy effects on the expression of VEGF, IL 6 and reactive oxygen species were investigated. RESULTS: After exposure to hyperbaric oxygen, cell culturess showed a significant increase in the expression of VEGF after 3 and 5 days. All cultures showed significantly reduced formation of reactive oxygen species throughout the experiments. The expression of IL-6 decreased during the experiment in mono-cultures of human adipose derived stem cells and co-cultures. In contrast, mono-cultures of human skin fibroblasts showed an overall significantly increased expression of IL-6. CONCLUSIONS: Hyperbaric oxygen therapy leads to immunmodulatory and proangiogenetic effects in a wound-like enviroment of adipose derived stem cells and fibroblasts.

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