scholarly journals Deschampsia antarctica E. Desv. plants with different chromosome number cultivated in vitro. Probabilistic relations of three adaptability indices with genome size

1970 ◽  
pp. 293-298
Author(s):  
N. Yu. Miryuta ◽  
I. Yu. Parnikoza ◽  
O. O. Poronnik ◽  
G. Yu. Myryuta ◽  
V. A. Kunakh
Keyword(s):  
Genome Size ◽  
Leaf Length ◽  
Cultivation Condition ◽  
Deschampsia Antarctica ◽  
Plant Leaves ◽  
Electrophoretic Method ◽  
In Vitro Methods ◽  
Special Information

Aim. This work deals with probabilistic relations between genome size and adaptability indices research in 11 genotypes plants revealed among seeds of Deschampsia antarctica E. Desv. which has different genome size under standardized cultivation condition in vitro. Methods. We have used electrophoretic method for determination of protein relative contents in plant leaves, and statistical analysis for probabilistic relations determination. Results. Protein densitometric profiles for 11 D. antarctica genotypes plants were analyzed. Probabilistic scheme have been created for all researched plants genotypes for all previously analyzed parameters. Three probabilistic schemes of genotypes plants received from seeds collected in the same loci and season have been shown and analyzed. Conclusions. Protective protein densitometric profiles were similar for 11 genotypes of D. antarctica plants under standardized cultivation condition in vitro but quantitative part of protective protein has been changed. Genome size had an influence on its probabilistic relations with flavonoids content, leaf length and some protein relative contents in general pool. Probabilistic relations between genome size and some protein relative content and between the last and flavonoids content and leaf length is assumed to express underlying in this plants adaptation process to uniqueness nature condition in dynamic hereditary memory special information in certain degree. Mechanisms of probabilistic relations changes are required a detailed research.Keywords: Deschampsia antarctica, plants in vitro, probabilistic relations between genome size and adaptability indices, plant adaptability.

scholarly journals Deschampsia antarctica E. Desv. plants with different chromosome number cultivated in vitro. Relations between genome size and two adaptability indices

1970 ◽  
pp. 304-309
Author(s):  
I. Yu. Parnikoza ◽  
N. Yu. Miryuta ◽  
M. Roiek ◽  
A. A. Betiekhtin ◽  
O. O. Poronnik ◽  
...  
Keyword(s):  
Genome Size ◽  
Leaf Length ◽  
Cultivation Condition ◽  
Deschampsia Antarctica ◽  
Flow Cytometry Analysis ◽  
Plant Leaves ◽  
Biochemical Method ◽  
In Vitro Methods

Aim. We have researched probabilistic relations between genome size and two adaptability indices in eleven genotypes revealed in seeds germ of Deschampsia antarctica E. Desv. which has different genome size under standardized cultivation condition in vitro. Methods. We have used biochemical method for determination of flavonoid contents in plant leaves and flow cytometry analysis for determination of genome size in plant leaves. Results. Eleven genotypes of Deschampsia antarctica with different genome size have been researched by three data rows. Probabilistic scheme have been created and analyzed for all researched genotypes. Conclusions. The Deschampsia antarctica genome size or karyotype changes have been shown to have effect on its probabilistic relations with flavonoids content and leaf length. Mechanisms of such relations is required a detailed research. Keywords: Deschampsia antarctica, genome size, leaf length, flavonoids.

scholarly journals Deschampsia antarctica E. Desv. plants with different chromosome number cultivated in vitro. Plants length and flavonoids in in vitro culture and in nature

1970 ◽  
pp. 310-313 ◽  
Author(s):  
O. O. Poronnik ◽  
I. Yu. Parnikoza ◽  
N. Yu. Miryuta ◽  
G. Yu. Myryuta ◽  
V. P. Grakhov ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Hplc Method ◽  
Leaf Length ◽  
Cultivated Plants ◽  
Cultivation Condition ◽  
Deschampsia Antarctica ◽  
Plant Leaves ◽  
Flavonoid Content ◽  
Native Populations

Aim. The aim of this work is to research two adaptability indices (leaf length and flavonoid content and spectrum) in eleven genotypes revealed in seeds germ of Deschampsia antarctica E. Desv. in standardized cultivation condition in vitro. Then these adaptability indices would compare with the same for native populations. Methods. We have used biochemical method for determination of flavonoid contents in plant leaves, HPLC method for research of flavonoid spectrum in plant leaves. Results. Heterogeneity of leaf length and flavonoid content has been shown. Both parameters difference have been detected in comparison with native populations. Flavonoid spectrum has no difference in comparison with native populations. Conclusions. Deschampsia antarctica genotypes under standardized cultivation condition in vitro have had longer leaves than plants in nature populations. Length of the leaves was varied depending on karyotype of corresponding genotype. Cultivated plants have had flavonoid spectrum similar to the same plants in nature. This fact allows to assume plants in vitro possible producent of secondary metabolites. Keywords: Deschampsia antarctica, leaf length, flavonoids, chromosome number.

scholarly journals Calculation of United Quality Latent Indices of Deschampsia antarctica plants adaptability of different origin grown in vitro

2021 ◽  
pp. 56-81
Author(s):  
N. Miryuta ◽  
◽  
I. Parnikoza ◽  
O. Poronnik ◽  
G. Myryuta ◽  
...  
Keyword(s):  
Genome Size ◽  
Genetic Distances ◽  
Leaf Length ◽  
Dimensionless Number ◽  
Deschampsia Antarctica ◽  
In Vitro Cultivation ◽  
Genetic Characteristics ◽  
Auxin Metabolism ◽  
Correlation Models

The research was to develop and describe in detail the algorithm for calculating the United Quality Latent Index (UQLI, Iq ) of plant adaptability from the collection of Deschampsia antarctica Ė. Desv. genotypes obtained from seeds collected at different sites in the Argentine Islands region, the maritime Antarctic, and grown in vitro at the laboratory conditions. Genome size and genetic distances by ISSR and IRAP markers according to data from published articles were used as basic indices of initial genetic heterogeneity for analyzed plant genotypes. To assess individual adaptability indices for eleven D. antarctica genotypes, we used measurement of the leaf length morphometric index and determination of the flavonoids content by rutin and the content of photosynthetic pigments. The spectra of reserve and protective proteins in leaves were investigated by polyacrylamide gel electrophoresis. To obtain the United Quality Latent Index of Adaptability (Iqi, UQLI), the method of extreme grouping was used. The estimation of Iqi (UQLI) was performed using pairwise comparisons of indices from differences sets for each pair of genotypes. We developed and described in detail the algorithm for Iqi estimation for eleven D. antarctica genotypes. As an example of application, correlation models of probability relations of the indices are presented. To evaluate the complex adaptability for eleven D. antarctica genotypes grown in vitro we used developed algorithm for the UQLI calculation. The individuality of the adaptive portrait for all studied genotypes under in vitro cultivation conditions was shown. The influence of basic genetic characteristics (genome size and genetic distances) on auxin metabolism-related indices of leaf length and flavonoid content was shown. Such effect may be carried out by genetic characteristics both individually and together, probably via auxin metabolism. Among the eight genotypes researched, we distinguish four different variants by correlation models and two (positive and negative) by the general Iqi value. Thus the Iqi (UQLI) is proposed to describe a large number of source data at different organization levels which characterize sample genotypes by reducing the dimensions to one dimensionless number. This genotypes’ individuality and the peculiarities of their grouping by Iqi should be taken into account when doing experimental studies using these genotypes as model plants, especially in experiments studying the regulation of productivity and the effect of the various exogenous factors, etc.

Standardization of in-vitro methods for determination of antimicrobial properties of essential oils

2021 ◽  
Vol Publish Ahead of Print ◽  
Author(s):  
Iraj Rasooli ◽  
Horieh Saderi ◽  
Reza MohammadSalehi ◽  
Masoud Dadashi ◽  
Parviz Owlia
Keyword(s):  
Essential Oils ◽  
Antimicrobial Properties ◽  
In Vitro Methods

scholarly journals Mycotoxin bioaccessibility/absorption assessment using in vitro digestion models: a review

2013 ◽  
Vol 6 (2) ◽  
pp. 167-184 ◽  
Author(s):  
C.A. González-Arias ◽  
S. Marín ◽  
V. Sanchis ◽  
A.J. Ramos
Keyword(s):  
Animal Studies ◽  
In Vitro Digestion ◽  
Contamination Level ◽  
Food Matrix ◽  
Gi Tract ◽  
Cell Culture Techniques ◽  
In Vitro Methods

In the evaluation of the oral bioavailability of a mycotoxin, the first step is the determination of its bioaccessibility, i.e. the percentage of mycotoxin released from the food matrix during digestion in the gastrointestinal (GI) tract that could be absorbed through the intestinal epithelium. Different in vitro digestion models have been recently used for determination of bioaccessibility, thereby avoiding the use of more complex cell culture techniques or the use of animals in expensive in vivo experiments. In vitro methods offer an appealing alternative to human and animal studies. They usually are rapid, simple and reasonably low in cost, and can be used to perform simplified experiments under uniform and well-controlled conditions, providing insights not achievable in whole animal studies. The available in vitro methods for GI simulation differ in the design of the system, the composition of the physiological juices assayed, as well as in the use or not of intestinal microbiota. There are models that only simulate the upper part of the GI tract (mouth-stomach-small intestine), whereas other methods include the large intestine, so that the model chosen could have some influence on the bioaccessibility data obtained. Bioaccessibility depends on the food matrix, as well as on the contamination level and the way the food/feed is contaminated (spiked or naturally). This review focuses on the currently available data regarding in vitro digestion models for the study of the bioaccessibility or absorption of mycotoxins, detailing the characteristics of each digestion step and the importance of the physiological juices employed during digestion. The effect that different factors play on mycotoxin release from the food matrix in the GI tract is also considered, and existing data on bioaccessibility of the main mycotoxins are given.

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