scholarly journals Calculation of United Quality Latent Indices of Deschampsia antarctica plants adaptability of different origin grown in vitro

2021 ◽  
pp. 56-81
Author(s):  
N. Miryuta ◽  
◽  
I. Parnikoza ◽  
O. Poronnik ◽  
G. Myryuta ◽  
...  
Keyword(s):  
Genome Size ◽  
Genetic Distances ◽  
Leaf Length ◽  
Dimensionless Number ◽  
Deschampsia Antarctica ◽  
In Vitro Cultivation ◽  
Genetic Characteristics ◽  
Auxin Metabolism ◽  
Correlation Models

The research was to develop and describe in detail the algorithm for calculating the United Quality Latent Index (UQLI, Iq ) of plant adaptability from the collection of Deschampsia antarctica Ė. Desv. genotypes obtained from seeds collected at different sites in the Argentine Islands region, the maritime Antarctic, and grown in vitro at the laboratory conditions. Genome size and genetic distances by ISSR and IRAP markers according to data from published articles were used as basic indices of initial genetic heterogeneity for analyzed plant genotypes. To assess individual adaptability indices for eleven D. antarctica genotypes, we used measurement of the leaf length morphometric index and determination of the flavonoids content by rutin and the content of photosynthetic pigments. The spectra of reserve and protective proteins in leaves were investigated by polyacrylamide gel electrophoresis. To obtain the United Quality Latent Index of Adaptability (Iqi, UQLI), the method of extreme grouping was used. The estimation of Iqi (UQLI) was performed using pairwise comparisons of indices from differences sets for each pair of genotypes. We developed and described in detail the algorithm for Iqi estimation for eleven D. antarctica genotypes. As an example of application, correlation models of probability relations of the indices are presented. To evaluate the complex adaptability for eleven D. antarctica genotypes grown in vitro we used developed algorithm for the UQLI calculation. The individuality of the adaptive portrait for all studied genotypes under in vitro cultivation conditions was shown. The influence of basic genetic characteristics (genome size and genetic distances) on auxin metabolism-related indices of leaf length and flavonoid content was shown. Such effect may be carried out by genetic characteristics both individually and together, probably via auxin metabolism. Among the eight genotypes researched, we distinguish four different variants by correlation models and two (positive and negative) by the general Iqi value. Thus the Iqi (UQLI) is proposed to describe a large number of source data at different organization levels which characterize sample genotypes by reducing the dimensions to one dimensionless number. This genotypes’ individuality and the peculiarities of their grouping by Iqi should be taken into account when doing experimental studies using these genotypes as model plants, especially in experiments studying the regulation of productivity and the effect of the various exogenous factors, etc.

scholarly journals Deschampsia antarctica E. Desv. plants with different chromosome number cultivated in vitro. Relations between genome size and two adaptability indices

1970 ◽  
pp. 304-309
Author(s):  
I. Yu. Parnikoza ◽  
N. Yu. Miryuta ◽  
M. Roiek ◽  
A. A. Betiekhtin ◽  
O. O. Poronnik ◽  
...  
Keyword(s):  
Genome Size ◽  
Leaf Length ◽  
Cultivation Condition ◽  
Deschampsia Antarctica ◽  
Flow Cytometry Analysis ◽  
Plant Leaves ◽  
Biochemical Method ◽  
In Vitro Methods

Aim. We have researched probabilistic relations between genome size and two adaptability indices in eleven genotypes revealed in seeds germ of Deschampsia antarctica E. Desv. which has different genome size under standardized cultivation condition in vitro. Methods. We have used biochemical method for determination of flavonoid contents in plant leaves and flow cytometry analysis for determination of genome size in plant leaves. Results. Eleven genotypes of Deschampsia antarctica with different genome size have been researched by three data rows. Probabilistic scheme have been created and analyzed for all researched genotypes. Conclusions. The Deschampsia antarctica genome size or karyotype changes have been shown to have effect on its probabilistic relations with flavonoids content and leaf length. Mechanisms of such relations is required a detailed research. Keywords: Deschampsia antarctica, genome size, leaf length, flavonoids.

scholarly journals Deschampsia antarctica E. Desv. plants with different chromosome number cultivated in vitro. Probabilistic relations of three adaptability indices with genome size

1970 ◽  
pp. 293-298
Author(s):  
N. Yu. Miryuta ◽  
I. Yu. Parnikoza ◽  
O. O. Poronnik ◽  
G. Yu. Myryuta ◽  
V. A. Kunakh
Keyword(s):  
Genome Size ◽  
Leaf Length ◽  
Cultivation Condition ◽  
Deschampsia Antarctica ◽  
Plant Leaves ◽  
Electrophoretic Method ◽  
In Vitro Methods ◽  
Special Information

Aim. This work deals with probabilistic relations between genome size and adaptability indices research in 11 genotypes plants revealed among seeds of Deschampsia antarctica E. Desv. which has different genome size under standardized cultivation condition in vitro. Methods. We have used electrophoretic method for determination of protein relative contents in plant leaves, and statistical analysis for probabilistic relations determination. Results. Protein densitometric profiles for 11 D. antarctica genotypes plants were analyzed. Probabilistic scheme have been created for all researched plants genotypes for all previously analyzed parameters. Three probabilistic schemes of genotypes plants received from seeds collected in the same loci and season have been shown and analyzed. Conclusions. Protective protein densitometric profiles were similar for 11 genotypes of D. antarctica plants under standardized cultivation condition in vitro but quantitative part of protective protein has been changed. Genome size had an influence on its probabilistic relations with flavonoids content, leaf length and some protein relative contents in general pool. Probabilistic relations between genome size and some protein relative content and between the last and flavonoids content and leaf length is assumed to express underlying in this plants adaptation process to uniqueness nature condition in dynamic hereditary memory special information in certain degree. Mechanisms of probabilistic relations changes are required a detailed research.Keywords: Deschampsia antarctica, plants in vitro, probabilistic relations between genome size and adaptability indices, plant adaptability.

scholarly journals Initial Growth of Seedlings of Diferent Pineapple Cultivars in Boa Vista, RR

2020 ◽  
Vol 24 (1) ◽  
pp. 41-46
Author(s):  
Deyse Cristina Oliveira Da Silva ◽  
Sandra Catia Pereira Uchôa ◽  
José Maria Arcanjo Alves ◽  
Valdinar Ferreira Melo ◽  
Cineone Nascimento Da Silva ◽  
...  
Keyword(s):  
Climatic Conditions ◽  
Leaf Length ◽  
Export Market ◽  
In Vitro Cultivation ◽  
Initial Growth ◽  
Vegetative Development ◽  
Percentage Survival ◽  
Randomized Design ◽  
Fruit Growing

Atualmente, no Brasil, o cultivo do abacaxizeiro, em escala comercial, é quase que exclusivamente com as cultivares Smooth Cayenne e Pérola, no entanto, ambas são suscetíveis a fusariose. A inserção de novas cultivares, com aceitação no mercado de exportação e resistente à fusariose é imprescindível para o sucesso da cultura. O objetivo deste estudo foi avaliar o desenvolvimento vegetativo precoce de cinco cultivares de abacaxi derivadas de micropropagação (BRS Ajubá, BRS Imperial, IAC Fantástico, Pérola e BRS Vitória). As mudas foram produzidas e aclimatizadas no Laboratório de Biotecnologia Biomudas, localizado em Venda Nova do Imigrante – ES, sendo posteriormente transportadas para Boa Vista, RR para implantação do experimento, onde foram conduzidas à casa de vegetação, sob condições climáticas controladas. Utilizou-se o delineamento inteiramente casualizado, em esquema de parcelas subdivididas, sendo cinco cultivares de abacaxizeiro (BRS Ajubá, IAC Fantástico, BRS Imperial, Pérola e BRS Vitória), oriundas de micropropagação, aleatorizadas nas parcelas e cinco épocas de avaliação (30, 60, 90, 120 e 150 dias após o transplantio - DAT) nas subparcelas, com quinze repetições. As variáveis de desenvolvimento vegetativo avaliadas foram: altura da planta, diâmetro da roseta, número de folhas, comprimento e largura de folha ”D”, além da percentagem de sobrevivência, ao final do experimento. As cultivares IAC Fantástico e Pérola apresentaram maior desenvolvimento vegetativo em relação à BRS Ajubá, BRS Imperial e BRS Vitória. As cultivares IAC Fantástico e Pérola, em razão da maior precocidade, podem ser transplantadas para local definitivo a partir de 30 DAT das mudas em casa de vegetação, as demais cultivares requerem 150 DAT. Palavras-chave: Aclimatação de Plantas. Crescimento de Mudas. Cultivo in vitro. Fruticultura. Micropropagação de Plantas.   Abstract Currently, the commercial pineapple cultivation in Brazil relies almost exclusively on the cultivars Smooth Cayenne and Pérola. However, both are susceptible to fusariosis. Develment of new cultivars, that are both acceptable to the export market and resistant to fusariosis, is essential for the continued crop success. The objective of this study was to evaluate early vegetative development of five micropropagation-derived pineapple cultivars (BRS Ajubá, BRS Imperial, IAC Fantástico, Pérola and BRS Vitória). Seedlings were produced and acclimatized at the Biomudas Biotechnology Laboratory, Venda Nova do Imigrante, Espírito Santo, Brazil, and were then transported to Boa Vista, Roraima, for the experiments. Here they were placed in a greenhouse under controlled climatic conditions and a completely randomized design implemented, using a subdivided plots scheme, with the micro-porpergation generated seedlings. Location randomized within plots, and evaluation running for five different periods (30, 60, 90, 120 and 150 days after transplant - DAT) in the subplots, with fifteen replicates. Evaluated vegetative development variables weere: plant height, rosette diameter, number of leaves, "D" leaf length and width, as well as percentage survival at experiment end. Cultivars IAC Fantástico and Pérola showed greater vegetative development than BRS Ajubá, BRS Imperial and BRS Vitória. The cultivars IAC Fantástico and Pérola, due to their greater precocity, can be transplanted to a final growth location 30 days after seedling transplantion into a greenhouse, while other cultivars require 150 DAT occur. Keywords: Plants Acclimation . Seedlings Growth. In vitro Cultivation. Fruit Growing. Pineapple Micropropagation .    

scholarly journals Iron concentrations in the in vitro cultivation of native Brazilian orchid Schomburgkia crispa

2018 ◽  
Vol 4 (2) ◽  
pp. 93
Author(s):  
Douglas Bertoncelli ◽  
Guilherme Alves ◽  
Gustavo Freiria ◽  
Felipe Furlan ◽  
Helio Neto ◽  
...  
Keyword(s):  
Root Length ◽  
Iron Concentration ◽  
Leaf Length ◽  
In Vitro Cultivation ◽  
Nutritional Requirement ◽  
Initial Development ◽  
Shoot Height ◽  
Shoot Number ◽  
Number Of Leaves

In vitro cultivation is a highly important biotechnological method widely used for the production of orchid seedlings, but it is necessary to study the suitability of the nutrients used in different kinds of formulation, as the nutritional requirement varies according to the species. The objective was to evaluate different concentrations of iron in the in vitro cultivation of Schomburgkia crispa Lindl seedlings. Seedlings were obtained from seeds germinated in vitro. Modified MS culture medium was used with half of the macronutrient concentration. The micronutrients were added according to the original formulation, except for the iron which was added from a stock solution of FeEDTA (FeSO4.7H2O: 5.6 g L-1 and EDTA: 7.48 g L-1) at 0.0; 2.5; 5.0; 7.5; 10.0 and 12.5mL L-1. At 200 days after seedling transplantation, shoot height, root length, number of leaves, shoot number, leaf length, leaf width, aerial and root dry mass, chlorophyll a, b and carotenoids content were evaluated. A completely randomized design was used, with six treatments and ten replicates of five seedlings. Regression analysis was performed at 5% of significance. The increase in iron concentration caused a reduction in root length and an increase in the number of leaves and shoots. The concentration of 4.13 mL L-1 of FeEDTA was the one that provided the best in vitro growth of S. crispa plants. High concentrations of iron caused a reduction of initial development, but stimulated an increase in the number of shoots.

scholarly journals Deschampsia antarctica E. Desv. plants with different chromosome number cultivated in vitro. Plants length and flavonoids in in vitro culture and in nature

1970 ◽  
pp. 310-313 ◽  
Author(s):  
O. O. Poronnik ◽  
I. Yu. Parnikoza ◽  
N. Yu. Miryuta ◽  
G. Yu. Myryuta ◽  
V. P. Grakhov ◽  
...  
Keyword(s):  
Chromosome Number ◽  
Hplc Method ◽  
Leaf Length ◽  
Cultivated Plants ◽  
Cultivation Condition ◽  
Deschampsia Antarctica ◽  
Plant Leaves ◽  
Flavonoid Content ◽  
Native Populations

Aim. The aim of this work is to research two adaptability indices (leaf length and flavonoid content and spectrum) in eleven genotypes revealed in seeds germ of Deschampsia antarctica E. Desv. in standardized cultivation condition in vitro. Then these adaptability indices would compare with the same for native populations. Methods. We have used biochemical method for determination of flavonoid contents in plant leaves, HPLC method for research of flavonoid spectrum in plant leaves. Results. Heterogeneity of leaf length and flavonoid content has been shown. Both parameters difference have been detected in comparison with native populations. Flavonoid spectrum has no difference in comparison with native populations. Conclusions. Deschampsia antarctica genotypes under standardized cultivation condition in vitro have had longer leaves than plants in nature populations. Length of the leaves was varied depending on karyotype of corresponding genotype. Cultivated plants have had flavonoid spectrum similar to the same plants in nature. This fact allows to assume plants in vitro possible producent of secondary metabolites. Keywords: Deschampsia antarctica, leaf length, flavonoids, chromosome number.

scholarly journals Study of Phytophthora infestans Mont. de Bary isolates in the planting of potatoes

2021 ◽  
pp. 86-91
Author(s):  
N. V. Matsishina ◽  
P. V. Fisenko ◽  
O. A. Sobko ◽  
I. V. Kim ◽  
D. I. Volkov ◽  
...  
Keyword(s):  
Phytophthora Infestans ◽  
Molecular Genetic ◽  
Microscopic Analysis ◽  
Molecular Genetic Analysis ◽  
In Vitro Cultivation ◽  
Genetic Characteristics ◽  
Potato Varieties ◽  
Phytotoxic Activity ◽  
Wide Range

Relevance. One of the most common diseases of potatoes and other nightshade family species is late blight caused by a pathogenic oomycete of the Phytophthora infestans (Mont.) de Bary. At least 100 species of phytophthora have been described in nature, affecting a wide range of plant species. The phytophthora population is heterogeneous and is represented by races, as well as different types of mating. This leads to a rapid adaptation of the pathogen and the emergence of new, more aggressive, and resistant races. Phytophthora is a parasite, the damage from which cannot be avoided within the organic farming framework. Therefore, it is particularly important to know the pathogenesis and racial composition of phytophthora in each individual region of Solanaceae cultivation.Research methodology. Differentiation and collection of material from the natural population were carried out using potato varieties with known R-genes in the genome. Isolation and introduction into the culture were carried out from leaves with the dampening chambers method, followed by cultivation on nutrient media. The pathogen was identified by microscopic analysis. Culture filtrates were obtained on the liquid nutritious medium, followed by liquid filtration and autoclaving. Phytotoxic activity was determined by the effect on the seedlings of the nightshade, grass, and pea families by the standard method. Molecular genetic analysis of the isolates was carried out by ISSR analysis; the primer, amplification mixture, and temperature profile of the reaction were selected according to the literature data; the calculation of genetic characteristics was carried out using POPGENE software packages.Results. Samples of seven Phytophthora infestans isolates were collected and introduced into culture. As a result of in vitro cultivation, morphological differences were revealed, expressed in the structure and color of the mycelium, the shape of the colonies, the nature of sporulation, the color of the reverse, and the medium under the colonies. The genetic differences of the natural phytophthora material introduced into the culture, collected from potato varieties with single resistance genes (R1, R3, R4), were revealed. Differences in the phytotoxic activity of the studied isolates' cultural filtrates were revealed. The isolated isolates demonstrate differentiation at the phenotypic, genetic and physiological levels, which allows us to speak about their belonging to races.

scholarly journals Desenvolvimento in vitro da orquídea ameaça de extinção Laelia marginata Lindl. em meios de cultivo contendo diferentes relações nitrato/amônio

2017 ◽  
Vol 23 (4) ◽  
pp. 392-399
Author(s):  
Douglas Junior Bertoncelli ◽  
Guilherme Augusto Cito Alves ◽  
Rodrigo Thibes Hoshino ◽  
Gustavo Henrique Freiria ◽  
Felipe Favoretto Furlan ◽  
...  
Keyword(s):  
Root Length ◽  
Aerial Part ◽  
Culture Media ◽  
Dry Mass ◽  
Leaf Length ◽  
In Vitro Cultivation ◽  
Initial Development ◽  
Randomized Design ◽  
Vitro Development

In vitro cultivation is a very important method for the conservation of germplasm and the multiplication of endangered plants; however, studies on the adequacy of the nutrients used for a good development of each species are needed. The objective of this study was to evaluate the in vitro development of Laelia marginata Lindl. in culture media containing different nitrate/ ammonium ratios. Seedlings were obtained from seeds germinated in vitro. The treatments consisted of different nitrate/ ammonium ratios, with five proportions of NO3 - and NH4 + : T1-0/100; T2-25/75; T3-50/50; T4-75/25 and T5-100/0. At 200 days after seedling transplanting, height of the aerial part, root length, number of leaves, number of shoots, leaf length, leaf width, aerial and root dry mass and contents of chlorophyll a, b and carotenoids were evaluated. A completely randomized design was used, with five treatments and ten replicates. The combination of 50/50 nitrate/ammonium resulted in the highest values of aerial part and root length, dry mass of the aerial part and root, as well as leaf length and width. The proportion of 50/50 nitrate/ammonium resulted in the best initial development of L. marginata seedlings.

Thickness of endometrium: predictor of the effectiveness of IVF/ICSI programs (literature review)

GYNECOLOGY ◽  
2018 ◽  
Vol 20 (1) ◽  
pp. 113-116
Author(s):  
L A Bagdasaryan ◽  
I E Korneyeva
Keyword(s):  
Assisted Reproductive Technologies ◽  
Endometrial Thickness ◽  
Molecular Genetic ◽  
Uterine Cavity ◽  
Reproductive Technologies ◽  
Genetic Characteristics ◽  
Vitro Fertilization ◽  
Need To Evaluate ◽  
The Relationship

The aim of the study is to systematically analyze the data available in the modern literature on the relationship between endometrial thickness and the frequency of pregnancy in the program of assisted reproductive technologies (ART). Materials and methods. The review includes data from foreign and domestic articles found in PubMed on this topic. Results. The article presents data on the relationship between the thickness of the endometrium and the frequency of pregnancy in ART programs. The greatest number of studies is devoted to the evaluation of the relationship between the thickness of the endometrium and the frequency of pregnancy on the day of the ovulation trigger. Data are presented on the existence of a correlation between the thickness of the endometrium measured on the day of the ovulation trigger and the frequency of clinical pregnancy, as well as data on the need to evaluate the structure of the endometrium and the state of subendometric blood flow. The importance of multilayered (three-layered) endometrium as a prognostic marker of success in in vitro fertilization/intracytoplasmic sperm injection programs in the ovum is emphasized. The conclusion. The thickness of the endometrium can not be used as an argument for canceling the cycle or abolishing embryo transfer to the uterine cavity. Further studies in this direction are needed with a study of the morphological and molecular genetic characteristics of the endometrium, which in the future will allow us to evaluate the relationship between the thickness of the endometrium and the probability of pregnancy.

Research of carbohydrate type influence on Lonicera caerulea l. Morphogenetic potential in vitro

2020 ◽  
Vol 61 ◽  
pp. 54-60
Author(s):  
N. D. Orlova ◽  
D. A. Egorova
Keyword(s):  
Multiplication Factor ◽  
Lonicera Caerulea ◽  
Genetic Characteristics ◽  
Clonal Micropropagation ◽  
Morphogenetic Potential

The clonal micropropagation technique of valuable Lonicera caerulea L. varieties (Indigo Gem, Moskovskaya 23, Diana, Jugana) has been improved. The effect of various types and concentrations of carbohydrates on microshoots regeneration of the genus Lonicera L. representatives was studied. It is shown that the concentration of carbohydrates has the greatest effect on microshoots height and the type of carbohydrate nutrition has the greatest effect on multiplication factor. The type of carbohydrate nutrition (42 %) had the greatest influence on multiplication factor. Peculiarities of varieties, genetic characteristics interaction, carbohydrate nutrition type and concentration had a lesser effect (20 %, 14 % and 11 % respectively). The variety Moskovskaya 23 was characterized by the highest microshoots number (49.6 mm). The highest multiplication factor in the studied varieties, regardless of the type of carbohydrate nutrition, was observed at concentration of 40 g/l (11.57).

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