scholarly journals Genotypic features of morphogenetic reactions of varieties and hybrids F1 of winter soft wheat during various stages of androgenesis in vitro

2018 ◽  
Vol 22 ◽  
pp. 252-256 ◽  
Author(s):  
I. S. Zambriborshch ◽  
O. L. Shestopal ◽  
M. S. Boyko
Keyword(s):  
Winter Wheat ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Soft Wheat ◽  
F1 Hybrids ◽  
Green Plants ◽  
Ability To Regenerate ◽  
Androgenesis In Vitro ◽  
Soft Winter Wheat

Aim. Testing the haploproduction ability of 47 varieties and 143 F1 hybrids of winter bread wheat. Methods. In vitro culture of isolated anthers of wheat. The percentage of callus and regeneration of green plants for each genotype calculated as a percentage of the planted anthers. Results. The differences in the frequency of сallus induction and the ability to regenerate plants in the process of androgenesiss in vitro of winter soft wheat were detected. The range of variation haploproduction activiti was broad. The sign of "the formation of сallus" for varieties was in limited from 0 to 21.2 % and for hybrids – from 0 to 38.8 %; on the sign of "regeneration of green plants" for varieties – from 0 to 9.4 %, and for hybrids from 0 to 9.1 %. The positive effect of 1BL/1RS translocation on signs of haploproduction in anther culture Triticum aestivum L. Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed. It has been found that F1 hybrids have a higher potential production potential in anther culture than varieties. During the period from 2011 to 2017, the 580 lines of winter wheat were transferred to the breeding departments. Keywords: soft winter wheat, anther culture in vitro, callus, regeneration.

scholarly journals Stabilization of soft wheat selection material which is the result of crossing with wild relatives

2021 ◽  
Vol 28 ◽  
pp. 83-87
Author(s):  
I. S. Zambriborshch ◽  
O. L. Shestopal ◽  
T. P. Nargan ◽  
M. S. Chekalova
Keyword(s):  
Winter Wheat ◽  
Anther Culture ◽  
Callus Formation ◽  
Wild Relatives ◽  
Soft Wheat ◽  
Ability To Regenerate ◽  
Androgenesis In Vitro ◽  
Callus Regeneration ◽  
Soft Winter Wheat

Aim. Testing the haploproduction ability of 30 hybrids of winter soft wheat. Methods. In vitro culture of isolated anthers of wheat. The percentage of callus and regeneration of green plants for each genotype calculated as a percentage of the planted anthers. Results. The differences in the frequency of callus induction and the ability to regenerate plants in the process of androgenesiss in vitro of winter soft wheat were detected. The microspores of 17 of 30 hybrids formed callus by in vitro anther culture were shown. The intensity of one process was different: more than half of the genotypes (18 pcs.) were characterized by a low percentage of callus (from 0.10 to 1.0%), 6 genotypes - medium (from 1.0 to 3.0%), and three - high (4.36%; 15.11% and 15.81%, respectively). Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed Samples P26 and P27 showed the highest level of callus formation. The 10 green regenerating plants were obtained. Keywords: hybrids, soft winter wheat, anther culture in vitro, callus, regeneration.

scholarly journals The testing of haploproduction ability of soft winter wheat different hybrids in anther culture in vitro

2020 ◽  
Vol 26 ◽  
pp. 207-211
Author(s):  
I. S. Zambriborshch ◽  
O. L. Shestopal ◽  
M. S. Chekalova ◽  
E. A. Golub
Keyword(s):  
Winter Wheat ◽  
Anther Culture ◽  
Hybrid Population ◽  
Culture In Vitro ◽  
Green Plants ◽  
Ability To Regenerate ◽  
Androgenesis In Vitro ◽  
Soft Winter Wheat ◽  
Range Of Variation

Aim. Study the the efficiency of androgenesis in vitro in anther culture of different genotyps winter wheat. Methods. Obtaining of soft winter wheat double haploid lines by anther culture in vitro. The statistical methods. Results The ability to androgenesis in an in vitro anthers culture of 30 soft winter wheat genotypes was tested. The differences in the frequency of callus induction and the ability to regenerate plants in the process of androgenesiss in vitro of winter soft wheat were detected. The range of variation haploproduction activiti (from % of implanted anthers) was broad. The sign of "the formation of callus" was in limited from 0 to 13.2 % and on the sign of "regeneration of green plants" – from 0 to 1.8 %. The 126 green plants-regenerants were received. Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed. It is advisable to use the anthers from the F2 hybrid population plant as a donor material to create an effective technology for producing double haploids of soft winter wheat was shown. Keywords: wheat hybrid, anther culture in vitro, callus, regeneration.

scholarly journals The efficiency of androgenesis in vitro in anther culture of soft wheat winter varieties and their different generations hybrids

1970 ◽  
pp. 194-197
Author(s):  
I. S. Zambriborshch ◽  
O. L. Shestopal ◽  
M. S. Boiko ◽  
H. O. Dobrova ◽  
S. V. Ahafonova
Keyword(s):  
Winter Wheat ◽  
Anther Culture ◽  
Bread Wheat ◽  
Culture In Vitro ◽  
Winter Bread Wheat ◽  
Effective Technology ◽  
Androgenesis In Vitro ◽  
Soft Winter Wheat ◽  
Wheat Hybrids

Aim. To estimate the level of in vitro haploproduction androgenic ability of different generations populations (F1, F2) soft winter wheat hybrids of the same combination to maximize the use of biotechnology cereals. Methods. Obtaining of bread wheat double haploid lines by anther culture in vitro. The statistical methods. Results. The influence different generation of wheat hybrids on the processes of induction and regeneration in anther culture of winter bread wheat were studied. The 21 green plants-regenerants were received. Conclusions. The using of anthers of the second generation simple hybrids as donor material for the effective technology creation of doubled haploid of bread winter wheat was shown.Keywords: wheat, anther culture in vitro, callus, regeneration, generation simple hybrids.

scholarly journals Efficiency of anther culture technique in the production of wheat double haploids

2008 ◽  
pp. 35-40 ◽  
Author(s):  
Ankica Kondic-Spika ◽  
Borislav Kobiljski ◽  
Nikola Hristov
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Green Plant ◽  
Culture Technique ◽  
Average Yield ◽  
Green Plants ◽  
Double Haploids

The objective of the study was to investigate efficiency of anther culture in the production of spontaneous double haploids from randomly selected heterozygous genotypes of wheat (Triticum aestivum L.). Anthers of 20 F1 wheat combinations were grown in vitro on a modified Potato-2 medium. All of the examined genotypes have shown the ability to produce pollen calluses as well as to regenerate green plants. On average for the whole experiment material, 47.2 calluses were produced per 100 cultured anthers. The green plant regeneration ranged from 0.8 to 13.4 green plants per spike, with an overall mean of 5.8. From the total of 582 regenerated green plants, 47.9% (279) were spontaneous double haploids. The final average yield from the study was 2.8 double haploids per spike.

scholarly journals Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture

2020 ◽  
Vol 56 (No. 4) ◽  
pp. 150-158
Author(s):  
Osama Zuhair Kanbar ◽  
Csaba Lantos ◽  
Paul Karumba Chege ◽  
Erzsébet Kiss ◽  
János Pauk
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Genetic Material ◽  
Triticum Aestivum L ◽  
Average Value ◽  
Doubled Haploid Lines ◽  
Dh Lines

We investigated the anther culture (AC) efficiency of thirteen F<sub>4</sub> combinations of winter wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic entities, i.e. embryo-like structures (ELS), regenerated-, green-, albino-, and transplanted plantlets. The number of green plantlets per 100 anthers (GP/100A) varied from 0.36 to 24.74 GP/100A with a mean of 8.31 GP/100A. Albino plantlets (AP) occurred in each combination, ranging from 0.20 to 22.80 AP/100A with an average value of 5.59 AP/100A. Between 25–87.76 doubled haploid (DH) plants per 100 acclimatised plantlets (DH/100ADP), depending on the combination, with a mean of 59.74% were recovered. We have found the highest DH production in the combinations Béres/Midas, Kalász/Tacitus, Béres/Pamier, and Premio/5009. This improves remarkably the choice of basic genetic material in subsequent crossing programmes. These observations emphasise the usability and efficiency of in vitro AC in producing a large number of DH lines for breeding and the applied researches of winter wheat. Although albinism was found in each combination, it was mitigated by the in vitro AC application.

scholarly journals Androgenic responses of winter wheat (Triticum aestivum L.) combinations in in vitro anther culture

Genetika ◽  
2020 ◽  
Vol 52 (1) ◽  
pp. 335-350
Author(s):  
Osama Kanbar ◽  
Csaba Lantos ◽  
Erzsebet Kiss ◽  
Janos Pauk
Keyword(s):  
Winter Wheat ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Published Data ◽  
In Vitro Production ◽  
The Mean ◽  
Dh Lines ◽  
Vitro Production

The androgenic parameters were investigated by in vitro anther culture (AC) on nine F2-5 breeding combinations of winter wheat (Triticum aestivium L.). Each combination produced embryo-like structures (ELS), green plantlets, albino plantlets, transplanted plantlets and acclimatized plantlets, with respect to the number of anthers in AC. The number of AC-derived ELS was between 11.73 and 52.76 ELS/100 anthers with the mean of 26.22 ELS/100 anthers, out of which the number of regenerated green plantlets varied from 3.20 to 26.40 green plantlets/100 anthers and the mean was 9.76 green plantlets/100 anthers, while the number of transplanted plantlets ranged from 2.16 to 21.77 transplanted plantlets/100 anthers. Furthermore, the number of albinos/100 anthers was mitigated and varied between 0.72 and 6.20 albinos/100 anthers. We also studied the number of green and albino plantlets per 100 ELS. The rate of green plantlets per 100 ELS ranged between 14.81% and 64.01%, with the overall mean 33.59%, while the rate of albinos per 100 ELS ranged from 4.82% to 20.70% with the overall mean 11.93%. In our experiment, the rate of acclimatized plantlets (70.15-91.57%) depended mostly on the combination. This study asserted the importance of AC method in wheat for in vitro production of green plants. Although albinism was found in each combination, it did not hinder the production of green plantlets. The satisfying results were achieved in green plantlets production compared to the previously published data, but further improvement will be needed continuously, experiment by experiment. The generated acclimatized plantlets will be used in the wheat breeding program as doubled haploid (DH) lines.

scholarly journals High production of wheat double haploids via anther culture

Genetika ◽  
2007 ◽  
Vol 39 (3) ◽  
pp. 405-411
Author(s):  
Ankica Kondic-Sipka ◽  
Boris Kobiljski ◽  
Nikola Hristov
Keyword(s):  
Anther Culture ◽  
Triticum Aestivum L ◽  
Green Plant ◽  
F1 Hybrids ◽  
Regeneration Ability ◽  
Breeding Programs ◽  
High Production ◽  
Dh Lines ◽  
Wheat Lines

Androgenous and regeneration abilities of 14 randomly selected F1 hybrids of wheat (Triticum aestivum L.) were analyzed. Anthers were grown in vitro on a modified Potato-2 inductive medium. The hybrid NS111-95/Ana had the highest average values for androgenous capacity (33%) and callus yield (119%), while the hybrid NS 92-250/Tiha had the lowest values for these traits (9 and 21%, respectively). Seven genotypes (50%) had a frequency of green plants relative to the number of isolated anthers of over 10%, with the highest frequency of 21.3% (NS111-95/Sremica). This hybrid produced 12.8 doubled haploid (DH) lines per spike used for isolation. In the other genotypes, the number of produced DH lines per spike ranged from 1 (30?Sc.Smoc.88-89/Hays-2) to 11.2 (NS111-95/Ana). As half of the randomly selected genotypes exhibited high green plant regeneration ability and a high production of DH lines per spike, it can be concluded that in vitro anther culture can be successfully used in breeding programs for rapid production of homozygous wheat lines.

HAPLOID PLANTS (2n = 21) FROM in vitro ANTHER CULTURE OF TRITICUM AESTIVUM

1974 ◽  
Vol 16 (3) ◽  
pp. 697-700 ◽  
Author(s):  
Iris L. Craig
Keyword(s):  
Triticum Aestivum ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Haploid Plants

Nine haploids (2n = 21) of Triticum aestivum L. (cv. Pitic–62) were obtained by in vitro anther culture, utilizing the medium described by Ouyang et al. (1973).

Selectivity of auxin for induction and growth of callus from excised embryo of spring and winter wheat

1984 ◽  
Vol 62 (7) ◽  
pp. 1393-1397 ◽  
Author(s):  
M. D. Zhou ◽  
T. T. Lee
Keyword(s):  
Winter Wheat ◽  
Chinese Spring ◽  
Shoot Growth ◽  
Callus Formation ◽  
Triticum Aestivum L ◽  
Dichlorophenoxyacetic Acid ◽  
Naphthaleneacetic Acid ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Methoxybenzoic Acid

The callus-promoting activity of most commonly known as well as some rarely tested auxins was compared with that of 2,4-dichlorophenoxyacetic acid (2,4-D) for in vitro culture of the excised embryo of spring and winter wheat (Triticum aestivum L.), cv. Chinese Spring and cv. Fredrick. Different auxins in a concentration range from 1 to 50 μM showed widely different activities. Also the two wheat cultivars responded differently to the auxins. When rapid callus formation with limited root growth was used as the basis for comparison, 2-(2-methyl-4-chlorophenoxy)propionic acid (2-MCPP), α-naphthaleneacetic acid, 3,6-dichloro-2-methoxybenzoic acid (dicamba), 4-amino-3,5,6,trichloropicolinic acid (picloram), γ-(2,4-dichlorophenoxy)butyric acid, 2,4,5-trichlorophenoxyacetic acid, and 2,4,5-trichlorophenoxypropionic acid, in the order of effectiveness, were superior to 2,4,-D for callus induction from the embryo of 'Chinese Spring,' although the concentration required was higher than that of 2,4-D. For the winter wheat 'Fredrick,' however, only picloram, dicamba, and 2-MCPP performed as well as 2,4-D. All auxins tested promoted shoot growth; 2-methyl-4-chlorophenoxypropionic acid was most effective for 'Chinese Spring,' whereas picloram was most effective for 'Fredrick.'

Sign in / Sign up

Export Citation Format

Share Document