scholarly journals High production of wheat double haploids via anther culture

Genetika ◽  
2007 ◽  
Vol 39 (3) ◽  
pp. 405-411
Author(s):  
Ankica Kondic-Sipka ◽  
Boris Kobiljski ◽  
Nikola Hristov
Keyword(s):  
Anther Culture ◽  
Triticum Aestivum L ◽  
Green Plant ◽  
F1 Hybrids ◽  
Regeneration Ability ◽  
Breeding Programs ◽  
High Production ◽  
Dh Lines ◽  
Wheat Lines

Androgenous and regeneration abilities of 14 randomly selected F1 hybrids of wheat (Triticum aestivum L.) were analyzed. Anthers were grown in vitro on a modified Potato-2 inductive medium. The hybrid NS111-95/Ana had the highest average values for androgenous capacity (33%) and callus yield (119%), while the hybrid NS 92-250/Tiha had the lowest values for these traits (9 and 21%, respectively). Seven genotypes (50%) had a frequency of green plants relative to the number of isolated anthers of over 10%, with the highest frequency of 21.3% (NS111-95/Sremica). This hybrid produced 12.8 doubled haploid (DH) lines per spike used for isolation. In the other genotypes, the number of produced DH lines per spike ranged from 1 (30?Sc.Smoc.88-89/Hays-2) to 11.2 (NS111-95/Ana). As half of the randomly selected genotypes exhibited high green plant regeneration ability and a high production of DH lines per spike, it can be concluded that in vitro anther culture can be successfully used in breeding programs for rapid production of homozygous wheat lines.

scholarly journals Efficiency of anther culture technique in the production of wheat double haploids

2008 ◽  
pp. 35-40 ◽  
Author(s):  
Ankica Kondic-Spika ◽  
Borislav Kobiljski ◽  
Nikola Hristov
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Green Plant ◽  
Culture Technique ◽  
Average Yield ◽  
Green Plants ◽  
Double Haploids

The objective of the study was to investigate efficiency of anther culture in the production of spontaneous double haploids from randomly selected heterozygous genotypes of wheat (Triticum aestivum L.). Anthers of 20 F1 wheat combinations were grown in vitro on a modified Potato-2 medium. All of the examined genotypes have shown the ability to produce pollen calluses as well as to regenerate green plants. On average for the whole experiment material, 47.2 calluses were produced per 100 cultured anthers. The green plant regeneration ranged from 0.8 to 13.4 green plants per spike, with an overall mean of 5.8. From the total of 582 regenerated green plants, 47.9% (279) were spontaneous double haploids. The final average yield from the study was 2.8 double haploids per spike.

scholarly journals Generation of doubled haploid lines from winter wheat (Triticum aestivum L.) breeding material using in vitro anther culture

2020 ◽  
Vol 56 (No. 4) ◽  
pp. 150-158
Author(s):  
Osama Zuhair Kanbar ◽  
Csaba Lantos ◽  
Paul Karumba Chege ◽  
Erzsébet Kiss ◽  
János Pauk
Keyword(s):  
Triticum Aestivum ◽  
Winter Wheat ◽  
Anther Culture ◽  
Doubled Haploid ◽  
Genetic Material ◽  
Triticum Aestivum L ◽  
Average Value ◽  
Doubled Haploid Lines ◽  
Dh Lines

We investigated the anther culture (AC) efficiency of thirteen F<sub>4</sub> combinations of winter wheat (Triticum aestivum L.). The genotype dependency was assessed during the induction of the androgenic entities, i.e. embryo-like structures (ELS), regenerated-, green-, albino-, and transplanted plantlets. The number of green plantlets per 100 anthers (GP/100A) varied from 0.36 to 24.74 GP/100A with a mean of 8.31 GP/100A. Albino plantlets (AP) occurred in each combination, ranging from 0.20 to 22.80 AP/100A with an average value of 5.59 AP/100A. Between 25–87.76 doubled haploid (DH) plants per 100 acclimatised plantlets (DH/100ADP), depending on the combination, with a mean of 59.74% were recovered. We have found the highest DH production in the combinations Béres/Midas, Kalász/Tacitus, Béres/Pamier, and Premio/5009. This improves remarkably the choice of basic genetic material in subsequent crossing programmes. These observations emphasise the usability and efficiency of in vitro AC in producing a large number of DH lines for breeding and the applied researches of winter wheat. Although albinism was found in each combination, it was mitigated by the in vitro AC application.

scholarly journals Utilization of in Vitro Anther Culture in Spelt Wheat Breeding

Plants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 436 ◽  
Author(s):  
Csaba Lantos ◽  
Szandra Purgel ◽  
Katalin Ács ◽  
Bernadett Langó ◽  
Lajos Bóna ◽  
...  
Keyword(s):  
Anther Culture ◽  
Heading Date ◽  
Wheat Breeding ◽  
F1 Hybrids ◽  
Spelt Wheat ◽  
Advanced Line ◽  
Flour Yield ◽  
The Mean ◽  
Dh Lines

The efficiency of in vitro anther culture was screened in a full diallel population of four spelt wheat genotypes and ten F1 hybrids. Genotype dependency was observed based on the data of embryo-like structures (ELS), green-, albino plantlets. In the diallel population and ten F1 hybrids, the green plantlets production ranged from 13.75 to 85.00 and from 6.30 to 51.00, respectively. The anther culture-derived plants of F1 hybrids were grown up in the nursery. At the harvest, 436 spontaneous doubled haploid (DH) plants were identified among the 1535 anther culture-derived transplanted and grown up individual plants. The mean of spontaneous rediploidization was 28.4% which ranged from 9.76% to 54.24%. In two consecutive years, the agronomic values of ‘Tonkoly.pop1’ advanced line were compared with seven DH lines of ‘Tonkoly.pop1’ in the nursery. The DH lines achieved competitive values in comparison with ‘Tonkoly.pop1’ advanced line based on the 11 measured parameters (heading date, plant height, yield, hardness, width and length of seed, TKW, hulling yield, flour yield, protein and wet gluten content). These observations presage the efficient utilization of anther culture in spelt wheat breeding.

scholarly journals Genotypic features of morphogenetic reactions of varieties and hybrids F1 of winter soft wheat during various stages of androgenesis in vitro

2018 ◽  
Vol 22 ◽  
pp. 252-256 ◽  
Author(s):  
I. S. Zambriborshch ◽  
O. L. Shestopal ◽  
M. S. Boyko
Keyword(s):  
Winter Wheat ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Soft Wheat ◽  
F1 Hybrids ◽  
Green Plants ◽  
Ability To Regenerate ◽  
Androgenesis In Vitro ◽  
Soft Winter Wheat

Aim. Testing the haploproduction ability of 47 varieties and 143 F1 hybrids of winter bread wheat. Methods. In vitro culture of isolated anthers of wheat. The percentage of callus and regeneration of green plants for each genotype calculated as a percentage of the planted anthers. Results. The differences in the frequency of сallus induction and the ability to regenerate plants in the process of androgenesiss in vitro of winter soft wheat were detected. The range of variation haploproduction activiti was broad. The sign of "the formation of сallus" for varieties was in limited from 0 to 21.2 % and for hybrids – from 0 to 38.8 %; on the sign of "regeneration of green plants" for varieties – from 0 to 9.4 %, and for hybrids from 0 to 9.1 %. The positive effect of 1BL/1RS translocation on signs of haploproduction in anther culture Triticum aestivum L. Conclusions. Genotype-specific of microspores morphogenetic reactions of soft winter wheat in the process of androgenesis in vitro were revealed. It has been found that F1 hybrids have a higher potential production potential in anther culture than varieties. During the period from 2011 to 2017, the 580 lines of winter wheat were transferred to the breeding departments. Keywords: soft winter wheat, anther culture in vitro, callus, regeneration.

scholarly journals Androgenic responses of winter wheat (Triticum aestivum L.) combinations in in vitro anther culture

Genetika ◽  
2020 ◽  
Vol 52 (1) ◽  
pp. 335-350
Author(s):  
Osama Kanbar ◽  
Csaba Lantos ◽  
Erzsebet Kiss ◽  
Janos Pauk
Keyword(s):  
Winter Wheat ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Wheat Breeding ◽  
Published Data ◽  
In Vitro Production ◽  
The Mean ◽  
Dh Lines ◽  
Vitro Production

The androgenic parameters were investigated by in vitro anther culture (AC) on nine F2-5 breeding combinations of winter wheat (Triticum aestivium L.). Each combination produced embryo-like structures (ELS), green plantlets, albino plantlets, transplanted plantlets and acclimatized plantlets, with respect to the number of anthers in AC. The number of AC-derived ELS was between 11.73 and 52.76 ELS/100 anthers with the mean of 26.22 ELS/100 anthers, out of which the number of regenerated green plantlets varied from 3.20 to 26.40 green plantlets/100 anthers and the mean was 9.76 green plantlets/100 anthers, while the number of transplanted plantlets ranged from 2.16 to 21.77 transplanted plantlets/100 anthers. Furthermore, the number of albinos/100 anthers was mitigated and varied between 0.72 and 6.20 albinos/100 anthers. We also studied the number of green and albino plantlets per 100 ELS. The rate of green plantlets per 100 ELS ranged between 14.81% and 64.01%, with the overall mean 33.59%, while the rate of albinos per 100 ELS ranged from 4.82% to 20.70% with the overall mean 11.93%. In our experiment, the rate of acclimatized plantlets (70.15-91.57%) depended mostly on the combination. This study asserted the importance of AC method in wheat for in vitro production of green plants. Although albinism was found in each combination, it did not hinder the production of green plantlets. The satisfying results were achieved in green plantlets production compared to the previously published data, but further improvement will be needed continuously, experiment by experiment. The generated acclimatized plantlets will be used in the wheat breeding program as doubled haploid (DH) lines.

scholarly journals Androgenous ability of heterozygous wheat genotypes and cytological characteristics of green regenerants

Genetika ◽  
2006 ◽  
Vol 38 (2) ◽  
pp. 153-158
Author(s):  
Branka Ljevnaic ◽  
Ankica Kondic-Sipka ◽  
Borislav Kobiljski ◽  
Srbislav Dencic
Keyword(s):  
Triticum Aestivum ◽  
Plant Regeneration ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Green Plant ◽  
Regeneration Ability ◽  
Cytological Analysis ◽  
Green Plants ◽  
Albino Plants ◽  
Cytological Characteristics

Androgenous and regeneration abilities of 8 heterozygous wheat (Triticum aestivum L.) genotypes were analyzed in anther culture. Cytological characteristics of green regenerants were also analyzed. Significant differences were found among the genotypes in their androgenous and regeneration abilities. The average androgenous capacity of all genotypes was 4.4% ranging from 0.7% (Prima/Hueguen) to 11.0% (NS173-98/NS164-98). The average callus yield was 8.7%. The genotype Banks/F53-70 produced the highest number of green plants (33 green plants), while the genotype Banks/Pobeda showed no green plant regeneration ability. The frequency of albino plants ranged between 0% (Banks/Rodna) and 5.3% (Anastasija/Radika). A total of 61 green and 61 albino plants were regenerated. The results of cytological analysis showed that, from the total number of regenerated green plants, 40.9% were haploids and 59.1% were spontaneous double haploids.

scholarly journals Response of some Tunisian bread wheat varieties (Triticum aestivum L.) to anther culture system

2018 ◽  
Vol 19 (1) ◽  
pp. 1-9
Author(s):  
Ali Ltifi ◽  
◽  
nissa Sahli ◽  
Wejden Brahmi ◽  
Insaf Nefzi ◽  
...  
Keyword(s):  
Anther Culture ◽  
Bread Wheat ◽  
Culture System ◽  
Culture Media ◽  
Triticum Aestivum L ◽  
Green Plant ◽  
Wheat Varieties ◽  
In Vitro Androgenesis ◽  
Good Number

This study focusedon wheat haploid production by anther culture. The main purpose wasto compare the in vitro androgenesis response often wheat Tunisian bread wheat varieties on two different induction media. The anthers were introduced in vitro when microspores were in the middle uninucleate stage and obtainedfrom treated spikes at 4° C for two weeks. The culture media 190-2 and BAC3 were used. Plants regenerated from induced embryos and calli were grown on the 190-2 medium without any growth regulators. The response to anther culture was different between the studied varieties. Androgenesis induction was better on the 190-2 medium than on BAC3 medium. In addition, the development of calli and the regeneration of embryos were better on 190-2 medium than on BAC3. Both green plantlets and albina were regenerated from induced embryos on the 190-2 medium, while no green plant regenerated from the embryos growingon BAC3 medium. Four varieties over ten studied gave good number of green plants. These results indicatedthat 190-2 medium was most favorable for the in vitro androgenesis of the Tunisian bread wheat varieties.

HAPLOID PLANTS (2n = 21) FROM in vitro ANTHER CULTURE OF TRITICUM AESTIVUM

1974 ◽  
Vol 16 (3) ◽  
pp. 697-700 ◽  
Author(s):  
Iris L. Craig
Keyword(s):  
Triticum Aestivum ◽  
Anther Culture ◽  
Triticum Aestivum L ◽  
Haploid Plants

Nine haploids (2n = 21) of Triticum aestivum L. (cv. Pitic–62) were obtained by in vitro anther culture, utilizing the medium described by Ouyang et al. (1973).

Genotype and Environment Strongly Influence Barley Anther Culture Response Using Australian Genotypes

1993 ◽  
Vol 41 (2) ◽  
pp. 227 ◽  
Author(s):  
SJ Logue ◽  
LC Giles ◽  
DHB Sparrow
Keyword(s):  
Anther Culture ◽  
Doubled Haploids ◽  
Growth Conditions ◽  
Green Plant ◽  
Optimal Conditions ◽  
Donor Plant ◽  
Breeding Programs ◽  
Barley Cultivars ◽  
Large Numbers ◽  
Anther Culture Response

A screening of several Australian barley cultivars of commercial interest has identified a number of genotypes that respond well to anther culture, with average levels of green plant regeneration between 23 and 134 plants/100 anthers cultured. Donor plant growth conditions have a large impact on anther culture response and, although optimal conditions for specific genotypes could possibly be identified, it is likely to be more effective for the production of large numbers of doubled haploids to settle for a broadly acceptable environment. Recent advances in methodology and the identification of responsive genotypes makes anther culture a feasible procedure for Australian barley breeding programs.

scholarly journals Chromosomal effects on In Vitro morphogenesis in Wheat intervarietal Substitution Lines

2011 ◽  
Vol 42 (No. 1) ◽  
pp. 15-19 ◽  
Author(s):  
N.D. Tyankova ◽  
N. Zagorska ◽  
V. Chardakov ◽  
A. Dryanova ◽  
B. Dimitrov
Keyword(s):  
Chinese Spring ◽  
Regeneration Ability ◽  
Substitution Lines ◽  
In Vitro Morphogenesis ◽  
Chromosome Substitutions ◽  
Allele Variation ◽  
Two Parameters ◽  
Wheat Lines ◽  
Intervarietal Substitution Lines

The effect of individual chromosomes on in vitro morphogenic ability of immature embryos was studied using a Chinese Spring/Timstein substitution series. For this purpose 20 wheat lines of Chinese Spring with consequently substituted chromosome pairs from the cultivar Timstein together with the two parental cultivars were investigated. The regeneration ability of the studied lines was quantified by two parameters: frequency of regenerative calli and coefficient of propagation. The influence of the 5A, 1B and 4D chromosomes on in vitro morphogenesis was determined and the effect of 2A, 3A, 3B, 4B, 6B and 1D chromosomes, reported by other authors, was confirmed. The data indicated that the intervarietal substitutions of certain chromosomes caused significant variation in the number of morphogenic calli and recovered regenerants, in spite of the fact that the two parental cultivars were of similar morphogenic ability. This probably reflects the allele variation in a given locus on these chromosomes and/or background effect. The data suggest that the chromosome substitutions may have different compensatory effects depending on the similarity of the corresponding chromosomes and also on the possibilities of their expression in an alien genetic background. &nbsp;

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