scholarly journals Genetic diversity of spring common wheat varieties at storage protein loci

2018 ◽  
Vol 23 ◽  
pp. 334-339
Author(s):  
T. O. Sobko ◽  
L. V. Sirant ◽  
G. M. Lisova

Aim. The aim of this study was to investigate genetic diversity of storage protein loci in spring common wheat varieties licensed for growing in Ukraine in 2017 year. Methods. SDS and APAG electrophoresis were used to identify genotypes at the gliadin loci Gli-1 and the high-molecular-weight glutenin subunit loci Glu-1. Results. Genotypes at the gliadin loci Gli A1, Gli B1, Gli D1 and glutenin loci Glu A1, Glu B1, Glu D1 were identified in 31 spring common wheat. The wheat-rye translocation AL/1RS was identified in two varieties Etyud and Struna mironivsyka. Differences in frequencies of alleles at storage protein loci were revealed. Conclusions. The high level of allelic variation was observed at the Gli-1 loci in spring common wheat varieties. Predominant alleles (one or two per locus) were revealed: Gli A1f, Gli A1а, Gli B1e, Gli D1b, Gli D1і, Gli D1f , Glu А1а, Glu Bс , Glu D1d, Glu D1a. Keywords: Triticum aestivum L., spring wheat, storage protein.

2019 ◽  
Vol 24 ◽  
pp. 284-289
Author(s):  
T. O. Sobko ◽  
G. M. Lisova ◽  
L. V. Sirant

Aim. The aim of the study was to investigate genetic variability of storage protein loci in European common winter wheat varieties licensed for growing in Ukraine in 2018 year. Methods. SDS and APAG electrophoresis were used to identify genotypes at the high-molecular-weight (HMW) glutenin loci Glu-1 and gliadin loci Gli-1. Results. Genotypes at the gliadin loci Gli-A1, Gli-B1, Gli-D1 and HMW glutenin loci Glu-A1, Glu-B1, Glu-D1 were identified in 48 winter common wheat varieties. A total of twenty gliadin alleles (including 10 at the Gli-A1, 4 at the Gli-B1, 6 at the Gli-D1 loci) and eleven HMW-GS alleles (including 3 at the Glu-A1, 5 at the Glu-B1, 3 at the Glu-D1 loci) were revealed. Differences in frequencies of alleles at storage protein loci were established. Conclusions. The high level of allelic variation was observed at the Gli-1 and Glu-1 loci in European common winter wheat varieties. Predominant alleles were revealed: Gli-A1о, Gli-A1а, Gli-A1f, Gli-B1f, Gli-D1b, Gli-D1d, Glu-А1c, Glu-А1a, Glu-B1с, Glu-B1d, Glu-D1d, Glu-D1a. Differences in frequencies of alleles at the storage protein loci between European and Ukrainian common winter wheat varieties were found. Keywords: Triticum aestivum L., winter wheat, storage protein, alleles.


2020 ◽  
Vol 27 ◽  
pp. 270-275
Author(s):  
T. O. Sobko ◽  
G. M. Lisova ◽  
L. V. Sirant

Aim. The aim of the study was to investigate genetic variability of gliadin-coding loci Gli-A1, Gli-B1, Gli-D1 in Ukrainian winter common wheat landraces and obsolete varieties. Methods. APAG electrophoresis of gliadin was used to identify genotypes at Gli-1 loci. Results. Diversity at the Gli-1 loci of 41 winter common wheat landraces, including local varieties Krymka, Banatka, Girka, Theyka, and 13 obsolete winter common wheat varieties, were studied. A total 31 gliadin alleles (including new 7 alleles) were revealed. There are 11 alleles at the Gli-A1 loci, 14 - at the Gli-B1, 6 -at the Gli-D1. Differences in frequencies of gliadin alleles were established. Conclusions. The high level of allelic variation at the Gli-1 loci was observed in Ukrainian winter common wheat landraces. Predominant alleles were revealed: Gli-A1m (46,8 %), Gli-A1d (21 %), Gli-B1b (47,1 %), Gli-D1g (45,8 %), Gli-D1a (33,3 %), Gli-D1j (13,9 %). In genotype of modern Ukrainian winter common wheat are present only 3 of them - Gli-B1b, Gli-D1g, Gli-D1j, which positively relate to dough quality. Keywords: Triticum aestivum L., winter wheat, landraces, gliadin , alleles , polymorphism


2010 ◽  
Vol 58 (4) ◽  
pp. 533-543 ◽  
Author(s):  
Alexandra Yu. Novoselskaya-Dragovich ◽  
Andrey V. Fisenko ◽  
Nikolay K. Yankovsky ◽  
Alexander M. Kudryavtsev ◽  
Qijian Yang ◽  
...  

2021 ◽  
Vol 16 (1) ◽  
pp. 641-652
Author(s):  
Sławomir Franaszek ◽  
Bolesław Salmanowicz

Abstract The main purpose of this research was the identification and characterization of low-molecular-weight glutenin subunit (LMW-GS) composition in common wheat and the determination of the effect of these proteins on the rheological properties of dough. The use of capillary zone electrophoresis and reverse-phase high-performance liquid chromatography has made it possible to identify four alleles in the Glu-A3 and Glu-D3 loci and seven alleles in the Glu-B3 locus, encoding LMW-GSs in 70 varieties and breeding lines of wheat tested. To determine the technological quality of dough, analyses were performed at the microscale using a TA.XT Plus Texture Analyzer. Wheat varieties containing the Glu-3 loci scheme (Glu-A3b, Glu-A3f at the Glu-A3 locus; Glu-B3a, Glu-B3b, Glu-B3d, Glu-B3h at the Glu-B3 locus; Glu-D3a, Glu-D3c at the Glu-D3 locus) determined the most beneficial quality parameters.


2016 ◽  
Vol 17 (4) ◽  
pp. 957-969
Author(s):  
T�mea KUTKA HLOZ�KOV� ◽  
Edita GREGOV� ◽  
Martin VIVOD�K ◽  
Zdenka G�lov�

2020 ◽  
Vol 8 (3) ◽  
pp. 80-87
Author(s):  
Yousif M. Fattah ◽  
Nergiz N. Tayib

Wheat (Triticum spp.) is one of the most important cereal crops in Iraq and the world. It includes many species and varieties.  The two major cultivated species of wheat are, durum wheat (Tritium durum Desf.) which is tetraploid (2n= 28) and the common wheat (Triticum aestivum L.) which is hexaploid (2n = 42). Ten wheat varieties from both species were examined using ten Simple   sequence repeat (SSR) markers (WMC17, WMC20, WMC21, WMC24, WMC25, WMC48, WMC50, WMC283, Xgwm11 and Xgwm626). Various genetic parameters were calculated using Power Marker V3.25 software. A total of 156 alleles were detected in both species. The gene diversity in wheat varieties from both species collectively varied from 0.85 to 1.00, which indicates considerable genetic diversity in the examined varieties. All markers used in this study were highly informative and the polymorphic information content (PIC) values were higher than 0.50 in all loci. Hence all markers are considered useful for genetic diversity studies in wheat’s populations. The dendrogram separated the populations into two main clades and many subgroups. Azadi variety was simplicifolious. This study confirms the discriminating power of SSR typing and its usefulness for comparison within hard and soft wheat populations. 


Euphytica ◽  
2008 ◽  
Vol 166 (2) ◽  
pp. 177-182 ◽  
Author(s):  
Jingye Fang ◽  
Yong Liu ◽  
Jie Luo ◽  
Yuesheng Wang ◽  
Peter R. Shewry ◽  
...  

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