scholarly journals Peer Review #3 of "Pore size is a critical parameter for obtaining sustained protein release from electrochemically synthesized mesoporous silicon microparticles (v0.1)"

2015 ◽  
Keyword(s):  
Pore Size ◽  
Peer Review ◽  
Critical Parameter ◽  
Protein Release ◽  
Mesoporous Silicon ◽  
Sustained Protein Release

scholarly journals Pore size is a critical parameter for obtaining sustained protein release from electrochemically synthesized mesoporous silicon microparticles

PeerJ ◽  
2015 ◽  
Vol 3 ◽  
pp. e1277 ◽  
Author(s):  
Ester L. Pastor ◽  
Elaine Reguera-Nuñez ◽  
Eugenia Matveeva ◽  
Marcos Garcia-Fuentes
Keyword(s):  
Particle Size ◽  
Pore Size ◽  
Internal Surface ◽  
Protein Release ◽  
Burst Release ◽  
Internal Surface Area ◽  
Release Process ◽  
Mesoporous Silicon ◽  
Model Protein ◽  
Sustained Protein Release

Mesoporous silicon has become a material of high interest for drug delivery due to its outstanding internal surface area and inherent biodegradability. We have previously reported the preparation of mesoporous silicon microparticles (MS-MPs) synthesized by an advantageous electrochemical method, and showed that due to their inner structure they can adsorb proteins in amounts exceeding the mass of the carrier itself. Protein release from these MS-MPs showed low burst effect and fast delivery kinetics with complete release in a few hours. In this work, we explored if tailoring the size of the inner pores of the particles would retard the protein release process. To address this hypothesis, three new MS-MPs prototypes were prepared by electrochemical synthesis, and the resulting carriers were characterized for morphology, particle size, and pore structure. All MS-MP prototypes had 90 µm mean particle size, but depending on the current density applied for synthesis, pore size changed between 5 and 13 nm. The model proteinα-chymotrypsinogen was loaded into MS-MPs by adsorption and solvent evaporation. In the subsequent release experiments, no burst release of the protein was detected for any prototype. However, prototypes with larger pores (>10 nm) reached 100% release in 24–48 h, whereas prototypes with small mesopores (<6 nm) still retained most of their cargo after 96 h. MS-MPs with ∼6 nm pores were loaded with the osteogenic factor BMP7, and sustained release of this protein for up to two weeks was achieved. In conclusion, our results confirm that tailoring pore size can modify protein release from MS-MPs, and that prototypes with potential therapeutic utility for regional delivery of osteogenic factors can be prepared by convenient techniques.

Injectable Acylhydrazone‐linked RAFT Polymer Hydrogels for Sustained Protein Release and Cell Encapsulation

2021 ◽  
pp. 2101284
Author(s):  
Fang‐Yi Lin ◽  
Nathan H. Dimmitt ◽  
Mariana Moraes de Lima Perini ◽  
Jiliang Li ◽  
Chien‐Chi Lin
Keyword(s):  
Cell Encapsulation ◽  
Protein Release ◽  
Polymer Hydrogels ◽  
Sustained Protein Release

Pore size engineering of mesoporous silicon nitride materials

2002 ◽  
Vol 4 (9) ◽  
pp. 1675-1681 ◽  
Author(s):  
Stefan Kaskel ◽  
Klaus Schlichte ◽  
Bodo Zibrowius
Keyword(s):  
Silicon Nitride ◽  
Pore Size ◽  
Mesoporous Silicon

Metal-chelating affinity hydrogels for sustained protein release

2007 ◽  
Vol 83A (4) ◽  
pp. 954-964 ◽  
Author(s):  
Chien-Chi Lin ◽  
Andrew T. Metters
Keyword(s):  
Protein Release ◽  
Metal Chelating ◽  
Sustained Protein Release
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