scholarly journals Peer Review #1 of "Growth and fatty acid characterization of microalgae isolated from municipal waste-treatment systems and the potential role of algal-associated bacteria in feedstock production (v0.1)"

2016 ◽  
Keyword(s):  
Fatty Acid ◽  
Peer Review ◽  
Potential Role ◽  
Waste Treatment ◽  
Municipal Waste ◽  
Associated Bacteria ◽  
Feedstock Production

scholarly journals Characterization of symbiotic and associated bacteria from entomopathogenic nematode Heterorhabditis sp. (nematode: Heterorhabditidae) isolated from India

2020 ◽  
Vol 30 (1) ◽  
Author(s):  
Rashid Pervez ◽  
Showkat Ahmad Lone ◽  
Sasmita Pattnaik
Keyword(s):  
Insect Pests ◽  
Entomopathogenic Nematode ◽  
Alcaligenes Faecalis ◽  
Symbiotic Bacteria ◽  
Rrna Gene ◽  
Main Body ◽  
Associated Bacteria ◽  
Molecular Approaches

Abstract Background Entomopathogenic nematodes (EPNs) harboring symbiotic bacteria are one of the safest alternatives to the chemical insecticides for the control of various insect pests. Infective juveniles of EPNs locate a target insect, enter through the openings, and reach the hemocoel, where they release the symbiotic bacteria and the target gets killed by the virulence factors of the bacteria. Photorhabdus with Heterorhabditis spp. are well documented; little is known about the associated bacteria. Main body In this study, we explored the presence of symbiotic and associated bacteria from Heterorhabditis sp. (IISR-EPN 09) and characterized by phenotypic, biochemical, and molecular approaches. Six bacterial isolates, belonging to four different genera, were recovered and identified as follows: Photorhabdus luminescens, one each strain of Providencia vermicola, Pseudomonas entomophila, Alcaligenes aquatilis, and two strains of Alcaligenes faecalis based on the phenotypic, biochemical criteria and the sequencing of 16S rRNA gene. Conclusion P. luminescens is symbiotically associated with Heterorhabditis sp. (IISR-EPN 09), whereas P. vermicola, P. entomophila, A. aquatilis, and A. faecalis are the associated bacteria. Further studies are needed to determine the exact role of the bacterial associates with the Heterorhabditis sp.

Characterization of Italian populations ofLoliumspp. resistant and susceptible to diclofop by inter simple sequence repeat

Weed Science ◽  
2004 ◽  
Vol 52 (4) ◽  
pp. 554-563 ◽  
Author(s):  
Giovanni Dinelli ◽  
Alessandra Bonetti ◽  
Ilaria Marotti ◽  
Maurizio Minelli ◽  
Pietro Catizone
Keyword(s):  
Potential Role ◽  
Inter Simple Sequence Repeat ◽  
Issr Markers ◽  
Intergeneric Hybridization ◽  
Banding Patterns ◽  
Inter Simple Sequence Repeats ◽  
Range Of Variation ◽  
Simple Sequence

Three ItalianLoliumweed populations, one susceptible and two resistant to diclofop, were characterized by the technique of inter simple sequence repeats (ISSR). The goal of this study was to taxonomically identify theseLoliumpopulations as well as to evaluate evidence for introgression of ISSR fragments fromFestucaand the potential role of this introgression in the diclofop response. ISSR analysis confirmed the genomic background of the weed populations to be consistent with that ofLolium. However, the great range of variation in ISSR banding patterns highlighted that the three ryegrass accessions are mixed populations made up of individuals resulting presumably from intrageneric and intergeneric hybridization in theLolium–Festucacomplex. TwoFestucagenus-discriminating and 20Festucaspecies-discriminating ISSR markers were screened among all the three ryegrass populations. The resistant Tuscania population carried the highest percentage ofFestucagenome (16.8%) followed by the resistant Roma (13.6%) and susceptible Vetralla (7.6%) populations. On the basis of these data some influence ofFestucagenome in diclofop resistance levels of studied ryegrass populations could be hypothesized.

Variation in the microbiome of the spider mite Tetranychus truncatus with sex, instar and endosymbiont infection

2020 ◽  
Vol 96 (2) ◽  
Author(s):  
Yu-Xi Zhu ◽  
Zhang-Rong Song ◽  
Shi-Mei Huo ◽  
Kun Yang ◽  
Xiao-Yue Hong
Keyword(s):  
Spider Mite ◽  
Potential Role ◽  
Developmental Stages ◽  
Spider Mites ◽  
Rrna Gene ◽  
Associated Bacteria ◽  
Operational Taxonomic Units ◽  
Dominant Bacteria ◽  
The 16S Rrna Gene

ABSTRACT Most arthropod-associated bacterial communities play a crucial role in host functional traits, whose structure could be dominated by endosymbionts. The spider mite Tetranychus truncatus is a notorious agricultural pest harboring various endosymbionts, yet the effects of endosymbionts on spider mite microbiota remain largely unknown. Here, using deep sequencing of the 16S rRNA gene, we characterized the microbiota of male and female T. truncatus with different endosymbionts (Wolbachia and Spiroplasma) across different developmental stages. Although the spider mite microbiota composition varied across the different developmental stages, Proteobacteria were the most dominant bacteria harbored in all samples. Positive relationships among related operational taxonomic units dominated the significant coassociation networks among bacteria. Moreover, the spider mites coinfected with Wolbachia and Spiroplasma had a significantly higher daily fecundity and juvenile survival rate than the singly infected or uninfected spider mites. The possible function of spider-mite associated bacteria was discussed. Our results highlight the dynamics of spider mite microbiotas across different life stages, and the potential role of endosymbionts in shaping the microbiota of spider mites and improving host fitness.

scholarly journals Growth and fatty acid characterization of microalgae isolated from municipal waste-treatment systems and the potential role of algal-associated bacteria in feedstock production

PeerJ ◽  
2016 ◽  
Vol 4 ◽  
pp. e1780 ◽  
Author(s):  
Kevin Stemmler ◽  
Rebecca Massimi ◽  
Andrea E. Kirkwood
Keyword(s):  
Fatty Acid ◽  
Environmental Sustainability ◽  
Municipal Wastewater ◽  
Unsaturated Fatty Acids ◽  
Waste Treatment ◽  
Commensal Bacteria ◽  
Biodiesel Production ◽  
Municipal Wastewater Treatment ◽  
Biofuel Feedstock ◽  
Feedstock Production

Much research has focused on growing microalgae for biofuel feedstock, yet there remain concerns about the feasibility of freshwater feedstock systems. To reduce cost and improve environmental sustainability, an ideal microalgal feedstock system would be fed by municipal, agricultural or industrial wastewater as a main source of water and nutrients. Nonetheless, the microalgae must also be tolerant of fluctuating wastewater quality, while still producing adequate biomass and lipid yields. To address this problem, our study focused on isolating and characterizing microalgal strains from three municipal wastewater treatment systems (two activated sludge and one aerated-stabilization basin systems) for their potential use in biofuel feedstock production. Most of the 19 isolates from wastewater grew faster than two culture collection strains under mixotrophic conditions, particularly with glucose. The fastest growing wastewater strains included the generaChlorellaandDictyochloris. The fastest growing microalgal strains were not necessarily the best lipid producers. Under photoautotrophic and mixotrophic growth conditions, single strains ofChlorellaandScenedesmuseach produced the highest lipid yields, including those most relevant to biodiesel production. A comparison of axenic and non-axenic versions of wastewater strains showed a notable effect of commensal bacteria on fatty acid composition. Strains grown with bacteria tended to produce relatively equal proportions of saturated and unsaturated fatty acids, which is an ideal lipid blend for biodiesel production. These results not only show the potential for using microalgae isolated from wastewater for growth in wastewater-fed feedstock systems, but also the important role that commensal bacteria may have in impacting the fatty acid profiles of microalgal feedstock.

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