Peer Review #2 of "Characterization of antimicrobial resistance genes in Haemophilus parasuis isolated from pigs in China (v0.2)"

Background: Haemophilus parasuis is a common porcine respiratory disease that causes high rates of morbidity and mortality in farmed swine. We performed a molecular characterization of antimicrobial resistance genes harbored by H. parasuis from pig farms in China. Methods: We screened 143 H. parasuis isolates for the presence of 64 antimicrobial resistance genes by PCR amplification and DNA sequence analysis. We determined quinolone resistance determining region mutations of DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE). The genetic relatedness among the strains was analyzed by pulsed-field gel electrophoresis. Results: We found 14 antimicrobial resistance genes were present in these isolates, including TEM-1, ROB-1.ermB,ermA ,flor, catl,tetB,tetC, rmtB, rmtD, aadA1, aac(3’)-ⅡC, sul1, and sul2 genes. Interestingly, one isolate carried 5 antibiotic resistance genes (tetB, tetC, flor, rmtB, sul1). The genes tetB, rmtB, and flor were the most prevalent resistance genes in H. parasuis in China. Alterations in the gyrA gene (S83F/Y, D87Y/N/H/G) were detected in 81% of the strains and parC mutations were often accompanied by a gyrA mutation. pulsed-field gel electrophoresis typing revealed 51 unique patterns in the isolates carrying antibiotic resistance genes indicating considerable genetic diversity and suggesting the genes were spread horizontally. Discussion: The current study demonstrated that the high antibiotic resistance of H. parasuis in piglets is a combination of transferable antibiotic resistance genes and multiple target gene mutations. GyrA gene mutation also was the most important role in quinolone resistance. These data provide novel insights for the better understanding of the prevalence and epidemiology of antimicrobial resistance in H. parasuis.

Download Full-text

Characterization of antimicrobial resistance genes in Haemophilus parasuis isolated from pigs in China

10.7287/peerj.preprints.3386 ◽

2017 ◽

Author(s):

Yongda Zhao ◽

Lili Guo ◽

Jie Li ◽

Xianhui Huang ◽

Binghu Fang

Keyword(s):

Antibiotic Resistance ◽

Antimicrobial Resistance ◽

Resistance Genes ◽

Gel Electrophoresis ◽

Antibiotic Resistance Genes ◽

Pulsed Field Gel Electrophoresis ◽

Haemophilus Parasuis ◽

Pulsed Field ◽

Antimicrobial Resistance Genes

Background: Haemophilus parasuis is a common porcine respiratory disease that causes high rates of morbidity and mortality in farmed swine. We performed a molecular characterization of antimicrobial resistance genes harbored by H. parasuis from pig farms in China. Methods: We screened 143 H. parasuis isolates for the presence of 64 antimicrobial resistance genes by PCR amplification and DNA sequence analysis. We determined quinolone resistance determining region mutations of DNA gyrase (gyrA and gyrB) and topoisomerase IV (parC and parE). The genetic relatedness among the strains was analyzed by pulsed-field gel electrophoresis. Results: We found 14 antimicrobial resistance genes were present in these isolates, including TEM-1, ROB-1.ermB,ermA ,flor, catl,tetB,tetC, rmtB, rmtD, aadA1, aac(3’)-ⅡC, sul1, and sul2 genes. Interestingly, one isolate carried 5 antibiotic resistance genes (tetB, tetC, flor, rmtB, sul1). The genes tetB, rmtB, and flor were the most prevalent resistance genes in H. parasuis in China. Alterations in the gyrA gene (S83F/Y, D87Y/N/H/G) were detected in 81% of the strains and parC mutations were often accompanied by a gyrA mutation. pulsed-field gel electrophoresis typing revealed 51 unique patterns in the isolates carrying antibiotic resistance genes indicating considerable genetic diversity and suggesting the genes were spread horizontally. Discussion: The current study demonstrated that the high antibiotic resistance of H. parasuis in piglets is a combination of transferable antibiotic resistance genes and multiple target gene mutations. GyrA gene mutation also was the most important role in quinolone resistance. These data provide novel insights for the better understanding of the prevalence and epidemiology of antimicrobial resistance in H. parasuis.

Download Full-text

Molecular and Phenotypic Characterization of Some Antimicrobial Resistance Genes in Escherichia coli Isolated from Human and Broiler Chickens

International Journal of Current Microbiology and Applied Sciences ◽

10.20546/ijcmas.2016.512.104 ◽

2016 ◽

Vol 5 (12) ◽

pp. 953-965

Author(s):

Mohamed Elmahdy Shehata ◽

Gamal Mohamed EL-Sherbiny ◽

Amira Hussainy Mohamed ◽

Hesham Mohamed Shafik

Keyword(s):

Escherichia Coli ◽

Antimicrobial Resistance ◽

Resistance Genes ◽

Broiler Chickens ◽

Phenotypic Characterization ◽

Antimicrobial Resistance Genes

Download Full-text

Characterization of antimicrobial resistance genes in Enterobacteriaceae carried by suburban mesocarnivores and locally owned and stray dogs

Zoonoses and Public Health ◽

10.1111/zph.12691 ◽

2020 ◽

Vol 67 (4) ◽

pp. 460-466

Author(s):

Katherine E. L. Worsley‐Tonks ◽

Elizabeth A. Miller ◽

Stanley D. Gehrt ◽

Shane C. McKenzie ◽

Dominic A. Travis ◽

...

Keyword(s):

Antimicrobial Resistance ◽

Resistance Genes ◽

Stray Dogs ◽

Antimicrobial Resistance Genes

Download Full-text

Detection and Characterization of Antimicrobial Resistance Genes in Pathogenic Bacteria *

Manual of Clinical Microbiology, 10th Edition ◽

10.1128/9781555816728.ch74 ◽

2011 ◽

pp. 1239-1261 ◽

Cited By ~ 1

Keyword(s):

Antimicrobial Resistance ◽

Resistance Genes ◽

Pathogenic Bacteria ◽

Antimicrobial Resistance Genes

Download Full-text

Genotypic antimicrobial resistance characterization of E. coli from dairy calves at high risk of respiratory disease administered enrofloxacin or tulathromycin

Scientific Reports ◽

10.1038/s41598-020-76232-w ◽

2020 ◽

Vol 10 (1) ◽

Author(s):

R. V. Pereira ◽

C. Foditsch ◽

J. D. Siler ◽

S. C. Dulièpre ◽

C. Altier ◽

...

Keyword(s):

High Risk ◽

Antimicrobial Resistance ◽

Respiratory Disease ◽

Resistance Genes ◽

Tetracycline Resistance ◽

Control Group ◽

E Coli ◽

Tetracycline Resistance Genes ◽

Antimicrobial Resistance Genes

Abstract The objective of this study was to evaluate the longitudinal effect of enrofloxacin or tulathromycin use in calves at high risk of bovine respiratory disease (BRD) on antimicrobial resistance genes and mutation in quinolone resistance-determining regions (QRDR) in fecal E. coli. Calves at high risk of developing BRD were randomly enrolled in one of three groups receiving: (1) enrofloxacin (ENR; n = 22); (2) tulathromycin (TUL; n = 24); or (3) no treatment (CTL; n = 21). Fecal samples were collected at enrollment and at 7, 28, and 56 days after beginning treatment, cultured for Escherichiacoli (EC) and DNA extracted. Isolates were screened for cephalosporin, quinolone and tetracycline resistance genes using PCR. QRDR screening was conducted using Sanger sequencing. The only resistance genes detected were aac(6′)Ib-cr (n = 13), bla-CTX-M (n = 51), bla-TEM (n = 117), tetA (n = 142) and tetB (n = 101). A significantly higher detection of gyrA mutated at position 248 at time points 7 (OR = 11.5; P value = 0.03) and 28 (OR = 9.0; P value = 0.05) was observed in the ENR group when compared to calves in the control group. Our findings support a better understanding of the potential impacts from the use of enrofloxacin in calves on the selection and persistence of resistance.

Download Full-text