scholarly journals Peer Review #3 of "Comparative phosphoproteome analysis to identify candidate phosphoproteins involved in blue light-induced brown film formation in Lentinula edodes (v0.2)"

2020 ◽  
Keyword(s):  
Peer Review ◽  
Blue Light ◽  
Lentinula Edodes ◽  
Film Formation

scholarly journals Comparative phosphoproteome analysis to identify candidate phosphoproteins involved in blue light-induced brown film formation in Lentinula edodes

PeerJ ◽  
2020 ◽  
Vol 8 ◽  
pp. e9859
Author(s):  
Tingting Song ◽  
Yingyue Shen ◽  
Qunli Jin ◽  
Weilin Feng ◽  
Lijun Fan ◽  
...  
Keyword(s):  
Blue Light ◽  
Molecular Mechanisms ◽  
Lentinula Edodes ◽  
Film Formation ◽  
Quantitative Information ◽  
Light Signal ◽  
Post Translational Modification ◽  
Bioinformatics Analyses ◽  
Phosphorylated Proteins ◽  
Liquid Chromatography Tandem Mass

Light plays an important role in the growth and differentiation of Lentinula edodes mycelia, and mycelial morphology is influenced by light wavelengths. The blue light-induced formation of brown film on the vegetative mycelial tissues of L. edodes is an important process. However, the mechanisms of L. edodes’ brown film formation, as induced by blue light, are still unclear. Using a high-resolution liquid chromatography-tandem mass spectrometry integrated with a highly sensitive immune-affinity antibody method, phosphoproteomes of L. edodes mycelia under red- and blue-light conditions were analyzed. A total of 11,224 phosphorylation sites were identified on 2,786 proteins, of which 9,243 sites on 2,579 proteins contained quantitative information. In total, 475 sites were up-regulated and 349 sites were down-regulated in the blue vs red group. To characterize the differentially phosphorylated proteins, systematic bioinformatics analyses, including gene ontology annotations, domain annotations, subcellular localizations, and Kyoto Encyclopedia of Genes and Genomes pathway annotations, were performed. These differentially phosphorylated proteins were correlated with light signal transduction, cell wall degradation, and melanogenesis, suggesting that these processes are involved in the formation of the brown film. Our study provides new insights into the molecular mechanisms of the blue light-induced brown film formation at the post-translational modification level.

Comparative phosphoproteome analysis to identify candidate phosphoproteins involved in blue light-induced brown film formation in Lentinula edodes

2020 ◽  
Author(s):  
Tingting Song ◽  
Yingyue Shen ◽  
Qunli Jin ◽  
Weilin Feng ◽  
Lijun Fan ◽  
...  
Keyword(s):  
Blue Light ◽  
Molecular Mechanisms ◽  
Lentinula Edodes ◽  
Film Formation ◽  
Quantitative Information ◽  
Light Signal ◽  
Post Translational Modification ◽  
Bioinformatics Analyses ◽  
Phosphorylated Proteins ◽  
Liquid Chromatography Tandem Mass

Abstract BackgroundLight plays an important role in the growth and differentiation of Lentinula edodes mycelia, and mycelial morphology is influenced by light wavelengths. The blue light-induced formation of brown film on the vegetative mycelial tissues of L. edodes is an important process. However, the mechanisms of L. edodes’ brown film formation, as induced by blue light, are still unclear. Using a high-resolution liquid chromatography-tandem mass spectrometry integrated with a highly sensitive immune-affinity antibody method, phosphoproteomes of L. edodes mycelia under red- and blue-light conditions were analyzed. ResultsA total of 11,224 phosphorylation sites were identified on 2,786 proteins, of which 9,243 sites on 2,579 proteins contained quantitative information. In total, 475 sites were up-regulated and 349 sites were down-regulated in the blue vs red group. To characterize the differentially phosphorylated proteins, systematic bioinformatics analyses, including gene ontology annotations, domain annotations, subcellular localizations, and Kyoto Encyclopedia of Genes and Genomes pathway annotations, were performed. These differentially phosphorylated proteins were correlated with light signal transduction, cell wall degradation, and melanogenesis, suggesting that these processes are involved in the formation of the brown film. ConclusionsOur study provides new insights into the molecular mechanisms of the blue light-induced brown film formation at the post-translational modification level.

Synergistic effects of antiwear and friction modifier additives

2020 ◽  
Vol 72 (8) ◽  
pp. 1019-1025
Author(s):  
Jennifer Eickworth ◽  
Enes Aydin ◽  
Martin Dienwiebel ◽  
Thomas Rühle ◽  
Patrick Wilke ◽  
...  
Keyword(s):  
Peer Review ◽  
Photoelectron Spectroscopy ◽  
Synergistic Effects ◽  
Film Formation ◽  
Wear Volume ◽  
Friction Modifier ◽  
Quartz Crystals ◽  
Content Type ◽  
Representative Study ◽  
Wear Reduction

Purpose Interactions of different additive types for antiwear/friction modification on surfaces can be synergistic or antagonistic in nature. This paper aims to investigate whether there are interactions between different additives in the adsorption process and whether they synergistic or antagonistic. The yielded correlations will be validated with tribological experiments to answer the question whether synergistic effects in adsorption also lead to synergistic effects in wear reduction. Design/methodology/approach In a representative study, zinc dialkyl-dithiophosphate and dithiophosphate were elaborated in combination with two different friction modifiers, a glycerol monooleate and an organic friction modifier. As base oils, mineral oil and poly alpha olefine were used. The adsorption behavior was studied via quartz crystal microbalance with dissipation using Fe2O3 coated quartz crystals. The tribological performance was evaluated in a ball-on-three disk tribometer. White light interferometry was used to determine the wear volume and X-ray photoelectron spectroscopy depth profiles of the tribofilms were obtained on selected systems. Findings The combination of dithiophosphate and an organic friction modifier (OFM) revealed a synergistic effect in terms of wear. If the initially formed films are viscoelastic, the third body formation during a tribo experiment is more pronounced and thereby wear can be reduced. As a mechanism, the adsorption of the OFM on the formed antiwear layer is proposed. Originality/value Correlating the analytical findings with performance experiments provides further understanding of the interactions between different constituents and their implications on film formation processes and wear reduction mechanisms. Peer review The peer review history for this article is available at: https://publons.com/publon/10.1108/ILT-07-2019-0293/

scholarly journals Transcriptome Analysis Identified Candidate Genes Involved in Fruit Body Development under Blue Light in Lentinula edodes

2021 ◽  
Vol 11 (15) ◽  
pp. 6997
Author(s):  
Dae Yeon Kim ◽  
Myoung-Jun Jang ◽  
Youn-Jin Park ◽  
Jae Yoon Kim
Keyword(s):  
Transcription Factor ◽  
Blue Light ◽  
Lentinula Edodes ◽  
Fruit Body ◽  
Light Condition ◽  
Gene Set Enrichment Analysis ◽  
Glycoside Hydrolases ◽  
Light Sources ◽  
Body Development ◽  
Fruit Body Development

Lentinula edodes is an edible mushroom that is rich in polysaccharides, glucan, and lentinan. It is famous for its earthy, sweet, umami flavor, and is used in various foods all over the world. Although Lentinula edodes does not carry out photosynthesis with light, its fruit body development is regulated by light. In this study, we analyzed the morphological changes of L. edodes strain Sanjo701ho and identified the global gene expression patterns using EdgeR of fruit body development under blue light. The phenotype analysis under different light sources revealed that the pileus diameter grew, while the stipe length was suppressed under blue light. To understand the changes in the transcriptome under different light sources in L. edodes, gene set enrichment analysis (GSEA), KOG functional categories, and KEGG pathways were used and compared to the no-light condition. Lignocellulose, CAZyme, and transcription factor classified DEGs (differentially expressed genes) were identified to better understand the significant DEGs affected by light sources in the synthesis, metabolism, and recognition of complex carbohydrates. Six glycoside hydrolases (GHs), four auxiliary activities (AAs), three carbohydrate esterases (CEs), and glycosyltransferases (GTs) were identified as upregulated in the CAZyme DEGs. Furthermore, four β-glucosidase, one glucose oxidase, and one multicopper oxidase-related gene for lignocellulolytic genes were upregulated in the blue light condition, and AT_hook transcription factor, CBFD_NFYB_HMF transcription factor, HMG_box transcription factor, and fungal specific transcription factor were upregulated in the blue light condition. This study helps us understand fruit body development in mushroom-breeding programs.

Temperature dependence of friction coefficient and transfer film formation in organic friction materials containing different abrasive components

2020 ◽  
Vol 72 (4) ◽  
pp. 483-489
Author(s):  
Jin Oh Chung ◽  
Sang Ryul Go ◽  
Hee Bum Choi ◽  
Tae Kwan Son
Keyword(s):  
Temperature Dependence ◽  
Peer Review ◽  
High Performance ◽  
Film Formation ◽  
Friction Material ◽  
Transfer Film ◽  
Friction Coefficients ◽  
Friction Materials ◽  
Content Type ◽  
Friction Temperature

Purpose This paper aims to investigate the temperature dependence of transfer film formation and friction coefficients in NAO friction materials with four different abrasive components, ZrO2, ZrSiO4, Al2O3 and Fe3O4. Design/methodology/approach 8.5% SnS2 was added as a lubricating component to friction materials. Friction tests comprised 100 times of consecutive braking application for each friction material under constant temperature of 300°C, 400°C, 500°C and 600°C. After the friction tests, the friction surfaces of the counterpart disks were examined by scanning electron microscope to access the formation of transfer film. Findings Coefficients of friction depended on not only friction temperature but also friction history which is related to development of transfer film. The effect of the transfer film formation was to reduce the friction coefficients for most friction materials. Quantities of the transfer film formation varied with friction materials; at low temperature below 400° the transfer film formation was most active in the Fe3O4 materials, while at 600° it was the most active in the Al2O3 material. The effect of the lubricating component SnS2 was to suppress the formation of transfer film, thus enhancing friction coefficients. Social implications The enhancement of friction coefficients with addition of small amount of lubricating components such as SnS2 is expected to open a new approach in developing high performance-brake pads. Originality/value Temperature was the controlling parameter in the present test. Under these test modes, transfer film could be fully developed to access the role of the transfer film. Peer review The peer review history for this article is available at: https://publons.com/publon/10.1108/ILT-10-2019-0427/

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