scholarly journals Genetic analysis of the Hungarian draft horse population using partial mitochondrial DNA D-loop sequencing

2017 ◽  
Author(s):  
Nikolett Csizmár ◽  
Sándor Mihók ◽  
András Jávor ◽  
Szilvia Kusza
Keyword(s):  
Nucleotide Diversity ◽  
Hypervariable Region ◽  
Conservation Strategies ◽  
Mixed Ancestry ◽  
Horse Population ◽  
Genetic Stocks ◽  
D Loop ◽  
Conservation Plan ◽  
Set Up

Background. Hungarian draft is a horse breed with a recent mixed ancestry. The interest in their conservation and characterization has increased over the last few years. It was developed in the 1920s by crossing local mares with draught horses imported from France and Belgium. The aim of this work is to contribute to the characterization of the endangered Hungarian heavy draft horse populations in order to obtain useful information to implement conservation strategies for these genetic stocks. Methods. To genetically characterize the breed and to set up the basis for a conservation programme, in this present study a hypervariable region of the mithocrondial DNA (D-loop) was used to assess genetic diversity in Hungarian draft horses. Two hundred and eighty five sequences obtained in our laboratory and 419 downloaded sequences available from Genbank were analyzed. Results. One hundred and sixty-four haplotypes were revealed. Thirty-six polymorphic sites were observed. High haplotype and nucleotide diversity values (Hd=0.954±0.004; π=0.028±0.0004) were identified in Hungarian populations, although they were higher within than among the total number of breeds (Hd =0.972±0.002; π=0.03097±0.002). Fourteen of the previously observed seventeen haplogroups were detected. Discussion. Our samples showed a large intra- and interbreed variation. There were no clear clustering on the median joining tree. The overall information given in this work led us to consider that the genetic scenario of this breed is more likely to be due to ‘ancestrally’ different genetic backrounds. This study could contribute to the development of a detailed breeding plan of Hungarian draft horse and help to formulate its genetic conservation plan, with the aim of increasing the population size, but avoiding inbreeding while, on the other hand, also facilitating genetic exchange among the populations.

scholarly journals Genetic analysis of the Hungarian draft horse population using partial mitochondrial DNA D-loop sequencing

2017 ◽  
Author(s):  
Nikolett Csizmár ◽  
Sándor Mihók ◽  
András Jávor ◽  
Szilvia Kusza
Keyword(s):  
Nucleotide Diversity ◽  
Hypervariable Region ◽  
Conservation Strategies ◽  
Mixed Ancestry ◽  
Horse Population ◽  
Genetic Stocks ◽  
D Loop ◽  
Conservation Plan ◽  
Set Up

Background. Hungarian draft is a horse breed with a recent mixed ancestry. The interest in their conservation and characterization has increased over the last few years. It was developed in the 1920s by crossing local mares with draught horses imported from France and Belgium. The aim of this work is to contribute to the characterization of the endangered Hungarian heavy draft horse populations in order to obtain useful information to implement conservation strategies for these genetic stocks. Methods. To genetically characterize the breed and to set up the basis for a conservation programme, in this present study a hypervariable region of the mithocrondial DNA (D-loop) was used to assess genetic diversity in Hungarian draft horses. Two hundred and eighty five sequences obtained in our laboratory and 419 downloaded sequences available from Genbank were analyzed. Results. One hundred and sixty-four haplotypes were revealed. Thirty-six polymorphic sites were observed. High haplotype and nucleotide diversity values (Hd=0.954±0.004; π=0.028±0.0004) were identified in Hungarian populations, although they were higher within than among the total number of breeds (Hd =0.972±0.002; π=0.03097±0.002). Fourteen of the previously observed seventeen haplogroups were detected. Discussion. Our samples showed a large intra- and interbreed variation. There were no clear clustering on the median joining tree. The overall information given in this work led us to consider that the genetic scenario of this breed is more likely to be due to ‘ancestrally’ different genetic backrounds. This study could contribute to the development of a detailed breeding plan of Hungarian draft horse and help to formulate its genetic conservation plan, with the aim of increasing the population size, but avoiding inbreeding while, on the other hand, also facilitating genetic exchange among the populations.

scholarly journals Genetic analysis of the Hungarian draft horse population using partial mitochondrial DNA D-loop sequencing

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e4198 ◽  
Author(s):  
Nikolett Csizmár ◽  
Sándor Mihók ◽  
András Jávor ◽  
Szilvia Kusza
Keyword(s):  
Hypervariable Region ◽  
Conservation Strategies ◽  
Mixed Ancestry ◽  
Horse Population ◽  
Genetic Stocks ◽  
D Loop ◽  
Conservation Plan ◽  
Hungarian Population ◽  
Different Populations ◽  
Set Up

Background The Hungarian draft is a horse breed with a recent mixed ancestry created in the 1920s by crossing local mares with draught horses imported from France and Belgium. The interest in its conservation and characterization has increased over the last few years. The aim of this work is to contribute to the characterization of the endangered Hungarian heavy draft horse populations in order to obtain useful information to implement conservation strategies for these genetic stocks. Methods To genetically characterize the breed and to set up the basis for a conservation program, in the present study a hypervariable region of the mitochrondial DNA (D-loop) was used to assess genetic diversity in Hungarian draft horses. Two hundred and eighty five sequences obtained in our laboratory and 419 downloaded sequences available from Genbank were analyzed. Results One hundred and sixty-four haplotypes and thirty-six polymorphic sites were observed. High haplotype and nucleotide diversity values (Hd = 0.954 ± 0.004; π = 0.028 ± 0.0004) were identified in Hungarian population, although they were higher within than among the different populations (Hd = 0.972 ± 0.002; π = 0.03097 ± 0.002). Fourteen of the previously observed seventeen haplogroups were detected. Discussion Our samples showed a large intra- and interbreed variation. There was no clear clustering on the median joining network figure. The overall information collected in this work led us to consider that the genetic scenario observed for Hungarian draft breed is more likely the result of contributions from ‘ancestrally’ different genetic backgrounds. This study could contribute to the development of a breeding plan for Hungarian draft horses and help to formulate a genetic conservation plan, avoiding inbreeding while.

scholarly journals Genetic diversity of the Hungarian draft horse assessed by mitochondrial DNA

2016 ◽  
pp. 29-32
Author(s):  
Nikolett Csizmár ◽  
Sándor Mihók ◽  
András Jávor ◽  
Szilvia Kusza
Keyword(s):  
Mitochondrial Dna ◽  
Nucleotide Diversity ◽  
Haplotype Frequency ◽  
Mixed Ancestry ◽  
D Loop ◽  
Breeding Programmes ◽  
Conservation Plan ◽  
Dna Fragment ◽  
Set Up ◽  
Endangered Breed

Hungarian draft is a horse breed with a recent mixed ancestry. It was developed in the 1920s by crossing local mares with draught horses imported from France and Belgium. To genetically characterize the breed and to set up the basis for a conservation programme, we have employed a molecular marker: a 256-bp D-loop mitochondrial DNA fragment. We analyzed 124 horses representing Hungarian draft horses to assess the maternal phylogeography of the breed. Sequence analysis of a 256-bp segment revealed a total of 34 haplotypes with thirty-four polymorphic sites. High haplotype and nucleotide diversity values (Hd=0.953±0.001; π=0.024±0.001) were detected. The average number of pairwise differences were k=5.998. This breed counts 800 mares today, and only survive due to breeding programmes, this way each haplotype frequency depends on the extent to which mares are involved into the breeding. The reduced number of surviving maternal lineages emphasizes the importance of establishing a conservation plan for this endangered breed. Due to the revealed 34 polymorphic sites we could presuppose twelve maternal linages, which could be a first step for making a breeding programme.

scholarly journals Maternal variability of Croatian Spotted goat (Capra hircus)

2019 ◽  
Vol 64 (No. 6) ◽  
pp. 248-254
Author(s):  
Ivana Drzaic ◽  
Ino Curik ◽  
Dinko Novosel ◽  
Vlatka Cubric-Curik
Keyword(s):  
Mitochondrial Dna ◽  
Nucleotide Diversity ◽  
Population Expansion ◽  
Capra Hircus ◽  
Phylogeographic Structure ◽  
Goat Breed ◽  
Loop Region ◽  
D Loop ◽  
Mtdna Diversity

Abstract: This study provides the first characterization of maternal ancestry and mitochondrial DNA (mtDNA) diversity in the Croatian Spotted goat (CSG), the most important autochthonous goat breed in Croatia. CSG (n = 25) were randomly sampled from seven herds and a 660-bp fragment from the mtDNA D-loop region was sequenced. Those sequences were compared with 122 corresponding GenBank sequences from goat populations in Albania, Austria, Egypt, Greece, Italy, Romania and Switzerland. CSG showed a great polymorphism (only three out of 17 haplotypes were shared) with high a haplotype (Hd = 0.967 ± 0.019) and nucleotide diversity (π = 0.01305 ± 0.00068). When compared with Mediterranean and ancient goats, all of the 25 CSG were randomly scattered inside haplogroup A showing the weak phylogeographic structure with within-breed variance accounting for 91.76% of the genetic variation. In addition, population expansion tests (mismatch distribution and Fu’s Fs statistic) supported these results suggesting at least one population expansion.

scholarly journals Mitochondrial D-loop sequence variation among Hucul horse

2013 ◽  
Vol 58 (No. 10) ◽  
pp. 437-442 ◽  
Author(s):  
V. Czerneková ◽  
T. Kott ◽  
I. Majzlík
Keyword(s):  
Mitochondrial Dna ◽  
Genetic Information ◽  
Sequence Variation ◽  
Dna Typing ◽  
Conservation Strategies ◽  
Base Pairs ◽  
Horse Population ◽  
Loop Region ◽  
Loop Sequence ◽  
D Loop

Genetic variation in the Czech Hucul horse population was analyzed using a sequence analysis of the D-loop region of mitochondrial DNA. One hundred and sixty-five Hucul horses were tested. Sequencing of the 700-base pairs fragment of the mitochondrial DNA D-loop region revealed 38 mutation sites representing 14 haplotypes, which were clustered into six haplogroups. The genetic information obtained from the mitochondrial DNA typing is of utmost importance for the future breed-conservation strategies.  

Rapid, Refined, and Robust Method for Expression, Purification, and Characterization of Recombinant Human Amyloid-beta M1-42

2019 ◽  
Author(s):  
Priya Prakash ◽  
Travis Lantz ◽  
Krupal P. Jethava ◽  
Gaurav Chopra
Keyword(s):  
Amyloid Beta ◽  
Western Blots ◽  
Force Microscopy ◽  
E Coli ◽  
Atomic Force ◽  
Set Up ◽  
Short Time

Amyloid plaques found in the brains of Alzheimer’s disease (AD) patients primarily consists of amyloid beta 1-42 (Ab42). Commercially, Ab42 is synthetized using peptide synthesizers. We describe a robust methodology for expression of recombinant human Ab(M1-42) in Rosetta(DE3)pLysS and BL21(DE3)pLysS competent E. coli with refined and rapid analytical purification techniques. The peptide is isolated and purified from the transformed cells using an optimized set-up for reverse-phase HPLC protocol, using commonly available C18 columns, yielding high amounts of peptide (~15-20 mg per 1 L culture) in a short time. The recombinant Ab(M1-42) forms characteristic aggregates similar to synthetic Ab42 aggregates as verified by western blots and atomic force microscopy to warrant future biological use. Our rapid, refined, and robust technique to purify human Ab(M1-42) can be used to synthesize chemical probes for several downstream in vitro and in vivo assays to facilitate AD research.

Isolation and characterization of oleochemical compounds from watermelon seed

2020 ◽  
Vol 10 (4) ◽  
pp. 79-82
Author(s):  
Madhubhushan M ◽  
Seshaiah S ◽  
Chandrudu J ◽  
Sagar R ◽  
Akila CR
Keyword(s):  
Cutting Tool ◽  
Citrullus Lanatus ◽  
Fatty Esters ◽  
Mineral Oils ◽  
Light Yellow ◽  
Isolation And Characterization ◽  
The Common ◽  
Set Up ◽  
Natural Gradation

The common watermelon item (Citrullus lanatus) seeds were gained from castoffs verdant nourishments for use by decorating, sun ventilation and pulverizing. Light yellow-toned oil was gotten by dissolvable withdrawal using oil atmosphere and the going with traits were gotten using oil ether: pH, refractive rundown, thickness, dissolvable miscibility, coagulating temperature, fire nature, express gravity, streak point and warmth of consuming. With a shallow level of unsaturation, stepped level of smoothness, and proximity of raised degree of the sensible proportion of free unsaturated fats. The low assessment of the solidifying temperature of the oil offered a hint that the oil can be managed in various areas paying little heed to the qualification in temperature. The following level of linoleic destructive of the oil offers a hint of natural gradation of solidarity. Fatty esters are increasing expanding significance as a biodegradable swap for mineral oils. In some request regions, for example, cutting tool oil, gearbox, pressure-driven oils, and greases for raw petroleum creation, the oleochemical items are set up. Nonetheless, certain particular wellsprings of fatty esters are hitherto to be abused for this comparative reason. This exploration subsequently tests into one of the less used wellsprings of fatty esters in watermelon. The oil from the kernels demonstrations a top-notch yield presents significant utilitarian gatherings for change and thus was utilized to set up an assortment of oleochemicals which demonstrated excellence materials in contrast with the routinely utilized oils feed frameworks for oleochemicals after portrayal. The photopolymers acquired indicated piercing vinyl protons for consistent polymerize.

Genetic gap analysis of wildHordeumtaxa

2012 ◽  
Vol 10 (3) ◽  
pp. 242-253 ◽  
Author(s):  
Holly Vincent ◽  
Roland von Bothmer ◽  
Helmut Knüpffer ◽  
Ahmed Amri ◽  
Jan Konopka ◽  
...  
Keyword(s):  
Gap Analysis ◽  
Plant Genetic Resources ◽  
Ex Situ ◽  
Conservation Strategies ◽  
Gene Pools ◽  
Barley Plant ◽  
Genetic Reserve ◽  
Set Up ◽  
Global Inventory

To facilitate the updating ofin situandex situconservation strategies for wild taxa of the genusHordeumL., a combined ecogeographic survey and gap analysis was undertaken. The analysis was based on the Global Inventory of Barley Plant Genetic Resources held by ICARDA plus additional datasets, resulting in a database containing 17,131 wildHordeumaccessions. The analysis concluded that a genetic reserve should be established in the Mendoza Province of Argentina, as this is the most species-rich area globally forHordeum. A network of reserves should also be set up across the Fertile Crescent in Israel, Palestine, Syria, Jordan, Lebanon and Turkey to provide effective conservation within the centres of diversity for gene pools 1B (Hordeum vulgaresubsp.spontaneum(C. Koch) Thell.) and 2 (Hordeum bulbosumL.). The majority of the species were deemed under-collected, so further collecting missions are required worldwide where possible. Althoughex situandin situconservation strategies have been developed, there needs to be further investigation into the ecological environments thatHordeumspecies occupy to ensure that any adaptive traits expressed are fully conserved. Additionally, studies are required to characterize existing collections and test the viability of rare species accessions held in genebanks to determine whether furtherex situcollections are required alongside the proposedin situconservation.

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