scholarly journals An ecological implication of glandular trichome-sequestered artemisinin: as a sink of biotic/abiotic stress-triggered singlet oxygen

2015 ◽  
Author(s):  
Jiang He ◽  
Qian Gao ◽  
Tao Liao ◽  
Qing-Ping Zeng
Keyword(s):  
Abiotic Stress ◽  
Singlet Oxygen ◽  
Artemisia Annua ◽  
Glandular Trichome ◽  
Stress Signaling ◽  
Ecological Implication ◽  
Oxygen Species ◽  
Ecological Implications ◽  
Dihydroartemisinic Acid ◽  
Artemisinin Biosynthesis

Artemisinin is accumulated in wormwood (Artemisia annua) with uncertain ecological implications. Here, we suggest that artemisinin is generated in response to biotic/abiotic stress, during which dihydroartemisinic acid, a direct artemisinin precursor, quenches singlet oxygen (1O2), one kind of reactive oxygen species. Evidence supporting artemisinin as a sink of 1O2 emerges from that volatile isoprenoids protect plants from biotic/abiotic stress; biotic/abiotic stress induces artemisinin biosynthesis; and stress signaling pathways are involved in the biosynthesis of volatile isoprenoids among plants as well as the biosynthesis of artemisinin in A. annua. In this review, we address the ecological implication of glandular trichome-sequestered artemisinin as a sink sink of biotic/abiotic stress-triggered 1O2 , and also summarize the cumulating data on the transcriptomic and metabolic profiling of stress-enhanced artemisinin production upon eliciting 1O2 omission from chloroplasts and initiating retrograde 1O2 signaling from chloroplasts to nuclei.

scholarly journals An ecological implication of glandular trichome-sequestered artemisinin: as a sink of biotic/abiotic stress-triggered singlet oxygen

2015 ◽  
Author(s):  
Jiang He ◽  
Qian Gao ◽  
Tao Liao ◽  
Qing-Ping Zeng
Keyword(s):  
Abiotic Stress ◽  
Singlet Oxygen ◽  
Artemisia Annua ◽  
Glandular Trichome ◽  
Stress Signaling ◽  
Ecological Implication ◽  
Oxygen Species ◽  
Ecological Implications ◽  
Dihydroartemisinic Acid ◽  
Artemisinin Biosynthesis

Artemisinin is accumulated in wormwood (Artemisia annua) with uncertain ecological implications. Here, we suggest that artemisinin is generated in response to biotic/abiotic stress, during which dihydroartemisinic acid, a direct artemisinin precursor, quenches singlet oxygen (1O2), one kind of reactive oxygen species. Evidence supporting artemisinin as a sink of 1O2 emerges from that volatile isoprenoids protect plants from biotic/abiotic stress; biotic/abiotic stress induces artemisinin biosynthesis; and stress signaling pathways are involved in the biosynthesis of volatile isoprenoids among plants as well as the biosynthesis of artemisinin in A. annua. In this review, we address the ecological implication of glandular trichome-sequestered artemisinin as a sink sink of biotic/abiotic stress-triggered 1O2 , and also summarize the cumulating data on the transcriptomic and metabolic profiling of stress-enhanced artemisinin production upon eliciting 1O2 omission from chloroplasts and initiating retrograde 1O2 signaling from chloroplasts to nuclei.

Early Events in Plant Abiotic Stress Signaling: Interplay Between Calcium, Reactive Oxygen Species and Phytohormones

2018 ◽  
Vol 37 (4) ◽  
pp. 1033-1049 ◽  
Author(s):  
Tapan Kumar Mohanta ◽  
Tufail Bashir ◽  
Abeer Hashem ◽  
Elsayed Fathi Abd_Allah ◽  
Abdul Latif Khan ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Abiotic Stress ◽  
Reactive Oxygen ◽  
Stress Signaling ◽  
Oxygen Species ◽  
Plant Abiotic Stress

GLANDULAR TRICHOME-SPECIFIC WRKY 1 promotes artemisinin biosynthesis in Artemisia annua

2016 ◽  
Vol 214 (1) ◽  
pp. 304-316 ◽  
Author(s):  
Minghui Chen ◽  
Tingxiang Yan ◽  
Qian Shen ◽  
Xu Lu ◽  
Qifang Pan ◽  
...  
Keyword(s):  
Artemisia Annua ◽  
Glandular Trichome ◽  
Artemisinin Biosynthesis

scholarly journals Chromatin Accessibility Is Associated with Artemisinin Biosynthesis Regulation in Artemisia annua

Molecules ◽  
2021 ◽  
Vol 26 (4) ◽  
pp. 1194
Author(s):  
Limeng Zhou ◽  
Yingzhang Huang ◽  
Qi Wang ◽  
Dianjing Guo
Keyword(s):  
Gene Expression ◽  
Artemisia Annua ◽  
Glandular Trichomes ◽  
Regulation Of Gene Expression ◽  
Glandular Trichome ◽  
Chromatin Accessibility ◽  
Biosynthetic Genes ◽  
Nuclear Molecules ◽  
Accessible Chromatin ◽  
Artemisinin Biosynthesis

Glandular trichome (GT) is the dominant site for artemisinin production in Artemisia annua. Several critical genes involved in artemisinin biosynthesis are specifically expressed in GT. However, the molecular mechanism of differential gene expression between GT and other tissue types remains elusive. Chromatin accessibility, defined as the degree to which nuclear molecules are able to interact with chromatin DNA, reflects gene expression capacity to a certain extent. Here, we investigated and compared the landscape of chromatin accessibility in Artemisia annua leaf and GT using the Assay for Transposase-Accessible Chromatin using sequencing (ATAC-seq) technique. We identified 5413 GT high accessible and 4045 GT low accessible regions, and these GT high accessible regions may contribute to GT-specific biological functions. Several GT-specific artemisinin biosynthetic genes, such as DBR2 and CYP71AV1, showed higher accessible regions in GT compared to that in leaf, implying that they might be regulated by chromatin accessibility. In addition, transcription factor binding motifs for MYB, bZIP, C2H2, and AP2 were overrepresented in the highly accessible chromatin regions associated with artemisinin biosynthetic genes in glandular trichomes. Finally, we proposed a working model illustrating the chromatin accessibility dynamics in regulating artemisinin biosynthetic gene expression. This work provided new insights into epigenetic regulation of gene expression in GT.

Reactive oxygen species in abiotic stress signaling

2010 ◽  
Vol 138 (4) ◽  
pp. 405-413 ◽  
Author(s):  
Pinja Jaspers ◽  
Jaakko Kangasjärvi
Keyword(s):  
Reactive Oxygen Species ◽  
Abiotic Stress ◽  
Reactive Oxygen ◽  
Stress Signaling ◽  
Oxygen Species

scholarly journals AaCycTL Regulates Cuticle and Trichome Development in Arabidopsis and Artemisia annua L.

2021 ◽  
Vol 12 ◽  
Author(s):  
Boran Dong ◽  
Xingxing Wang ◽  
Rui Jiang ◽  
Shiyuan Fang ◽  
Jinxing Li ◽  
...  
Keyword(s):  
Artemisia Annua ◽  
Glandular Trichome ◽  
Biosynthesis Pathway ◽  
Trichome Density ◽  
Trichome Development ◽  
Pathway Gene ◽  
Chinese Traditional Herb ◽  
Artemisinin Biosynthesis ◽  
Artemisinin Content ◽  
Important Drug

Artemisinin is an important drug for resistance against malaria. Artemisinin is derived from the glandular trichome of leaves, stems, or buds of the Chinese traditional herb Artemisia annua. Increasing the trichome density may enhance the artemisinin content of A. annua. It has been proven that cyclins are involved in the development of trichomes in tomato, Arabidopsis, and tobacco, but it is unclear whether the cyclins in A. annua influence trichome development. In this study, we showed that AaCycTL may regulate trichome development and affect the content of artemisinin. We cloned AaCycTL and found that it has the same expression files as the artemisinin biosynthesis pathway gene. We overexpressed AaCycTL in Arabidopsis, and the results indicated that AaCycTL changed the wax coverage on the surface of Arabidopsis leaves. The trichome density decreased as well. Using yeast two-hybrid and BiFC assays, we show that AaCycTL can interact with AaTAR1. Moreover, we overexpressed AaCycTL in A. annua and found that the expression of AaCycTL was increased to 82–195%. Changes in wax coverage on the surface of transgenic A. annua leaves or stems were found as well. We identified the expression of the artemisinin biosynthesis pathway genes ADS, CYP71AV1, and ALDH1 has decreased to 88–98%, 76–97%, and 82–97% in the AaCycTL-overexpressing A. annua lines, respectively. Furthermore, we found reduced the content of artemisinin. In agreement, overexpression of AaCycTL in A. annua or Arabidopsis may alter waxy loading, change the initiation of trichomes and downregulate trichome density. Altogether, AaCycTL mediates trichome development in A. annua and thus may serve to regulate trichome density and be used for artemisinin biosynthesis.

scholarly journals In silico identification of cis-Regulatory elements and their functional annotations from assembled ESTs of Artemisia annua L. involved in abiotic stress signaling

2021 ◽  
Vol 26 (2) ◽  
pp. 2384-2395
Author(s):  
PRAVEJ ALAM ◽  
◽  
THAMER AL BALAWI ◽  
Keyword(s):  
Salt Stress ◽  
Abiotic Stress ◽  
Artemisia Annua ◽  
Regulatory Elements ◽  
Common Side Effect ◽  
Stress Signaling ◽  
Functional Annotations ◽  
In Silico Identification ◽  
Plant Hormone Signaling ◽  
Artemisinin Content

Salt stress is a common side-effect in plants impacted on plant growth, metabolism and productivity. A. annua L. is one of the well-known antimalarial plants, biosynthesized artemisinin in its leaf, now introduced in all-over the world. In this article, we have analyzed the A. annua L. ESTs under salt stress and predicted cis-regulatory elements, roles in abiotic stress signaling. Further, the predicted abiotic stress responsive factors were analyzed in order to their function annotations as compare to the genome of Arabidopsis thaliana. 11 EST-contigs assembled from 127 were 29 signals elements were identified by CAP3 program. In order to evaluate accuracy of the identified factors, gene ontology functions were performed. GOBP analysis enriched the genes (85.71%) as the response to abiotic signaling. The co-expression analysis was revealed by gene investigators and String 10.0, these factors-oriented genes had at least 0.40 correlations and 0.7 mutual connection. In projected PPI network, the recognized factors belong to plant hormone signaling and diterpene pathways. These factors (ABF1, APX CCC1, CPK6, JAZ1, MYC2) introduced as candidate genes responsive factors could be overexpressed in A. annua L. plants either alone or in a shuttle may led the good metabolism and higher artemisinin content.

scholarly journals Artemisinin Biosynthesis in Non-glandular Trichome Cells of Artemisia annua

2019 ◽  
Vol 12 (5) ◽  
pp. 704-714 ◽  
Author(s):  
Rika Judd ◽  
M. Caleb Bagley ◽  
Mingzhuo Li ◽  
Yue Zhu ◽  
Caiyan Lei ◽  
...  
Keyword(s):  
Artemisia Annua ◽  
Glandular Trichome ◽  
Artemisinin Biosynthesis

Light-Induced Artemisinin Biosynthesis Is Regulated by the bZIP Transcription Factor AaHY5 in Artemisia annua

2019 ◽  
Vol 60 (8) ◽  
pp. 1747-1760 ◽  
Author(s):  
Xiaolong Hao ◽  
Yijun Zhong ◽  
Hans-Wilhelm N�tzmann ◽  
Xueqing Fu ◽  
Tingxiang Yan ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Leucine Zipper ◽  
Artemisia Annua ◽  
Glandular Trichome ◽  
Bzip Transcription Factor ◽  
Light Conditions ◽  
Basic Leucine Zipper ◽  
Ubiquitin E3 Ligase ◽  
Changing Light ◽  
Artemisinin Biosynthesis

Abstract Artemisinin, the frontline drug against malaria, is a sesquiterpenoid extracted from Artemisia annua. Light has been proposed to play an important role in the activation of artemisinin biosynthesis. Here, we report the basic leucine zipper transcription factor (TF) AaHY5 as a key regulator of light-induced biosynthesis of artemisinin. We show that AaHY5 transcription overlaps with that of artemisinin biosynthesis genes in response to light and in A. annua tissues. Analysis of AaHY5 overexpression and RNAi-suppression lines suggests that AaHY5 is a positive regulator of the expression of artemisinin biosynthesis genes and accumulation of artemisinin. We show that AaHY5 complements the hy5 mutant in Arabidopsis thaliana. Our data further suggest that AaHY5 interacts with AaCOP1, the ubiquitin E3 ligase CONSTITUTIVE PHOTOMORPHOGENIC1 in A. annua. In yeast one-hybrid and transient expression assays, we demonstrate that AaHY5 acts via the TF GLANDULAR TRICHOME-SPECIFIC WRKY 1 (AaGSW1) in artemisinin regulation. In summary, we present a novel regulator of artemisinin gene expression and propose a model in which AaHY5 indirectly controls artemisinin production in response to changing light conditions.

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