Effect of GA3 treatments and sowing conditions on ex situ seed germination of Oroxylum indicum (L) Benth. Ex Kurz: A threatened high value medicinal plant

Febjislami S, Kurniawati A, Melati M, Wahyu Y. 2019. Morphological characters, flowering and seed germination of the Indonesian medicinal plant Orthosiphon aristatus. Biodiversitas 20: 328-337. Orthosiphon aristatus (Blume) Miq is a popular medicinal plant in Southeast Asia. The morphological variation of O. aristatus is narrow and information on flowering and seed germination is limited. This study aimed to determine the morphological characters, flowering and seed germination of O. aristatus. The study was conducted on 19 accessions (ex situ collections) of O. aristatus from West, Central and East Java. It was found that the differences in morphological and flowering characters were mainly based on shape and color. The dominant stem color is strong yellowish green mixed with deep purplish pink in different proportions. The dominant leaf shape was medium elliptic. O. aristatus flower has three kinds of colors: purple, intermediate and white (the most common color). O. aristatus has heterostyled flower with a long-styled morph. The stigma has two shapes: open and close (the dominant shape). The open and close stigma was found in Tuban accession, the open stigma was found in Pamekasan accession. O. aristatus accessions have a narrow diversity of 84% similarity rate. O. aristatus started flowering at 4-5 weeks after planting and blooming 2-3 weeks later. O. aristatus seeds begin to germinate on the fourth day and take about eight days to grow from seedling to become complete sprouts. Count I ranged from day 5-6 and count II on days 10-11. Tuban accession has the highest germination rate of 72.97%. The results of this research can be considered as new information, regarding information about the diversity of morphological, flowering characters and the ability of O. aristatus seed to germinate.

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Ex-situ conservation of medicinal plant genetic resources managed by national agrobiodiversity center of Korea

Planta Medica ◽

10.1055/s-0032-1320321 ◽

2012 ◽

Vol 78 (11) ◽

Author(s):

JS Sung ◽

CW Jeong ◽

YY Lee ◽

HS Lee ◽

YA Jeon ◽

...

Keyword(s):

Medicinal Plant ◽

Genetic Resources ◽

Ex Situ Conservation ◽

Plant Genetic Resources ◽

Ex Situ

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Induction of Somatic Embryogenesis in Vulnerable Medicinal Plant Hedychium spicatum Buch-Ham ex Smith

Plant Tissue Culture and Biotechnology ◽

10.3329/ptcb.v23i2.17506 ◽

2014 ◽

Vol 23 (2) ◽

pp. 147-155 ◽

Cited By ~ 1

Author(s):

Dinesh Giri ◽

Sushma Tamta

Keyword(s):

Somatic Embryogenesis ◽

Medicinal Plant ◽

Somatic Embryos ◽

Synthetic Seeds ◽

Ex Situ ◽

Secondary Embryogenesis ◽

Zygotic Embryos ◽

High Concentration ◽

Cotyledon Explants ◽

Short Period

This protocol has been developed for somatic embryogenesis in Hedychium spicatum. Simultaneously, a method has also been developed for the production of synthetic seeds by using somatic embryos. Direct somatic embryos were developed on cotyledon explants of zygotic embryos on MS supplemented with high concentration of NAA (20.0 µM). Induction of secondary embryogenesis was best in 2,4-D supplemented medium fortified with activated charcoal. Germination of somatic embryos was enhanced by using GA3. Besides this, round and semi-hard beads of somatic embryos (synthetic seeds) could be produced by using 2% Na-alginate and 100 mM calcium chloride and more than 30% germination of synthetic seeds was achieved in MS. Well acclimated plants produced via somatic embryogenesis and/or synthetic seeds were transferred to field where more than 60% survived. This simple study enabled us to obtain a number of plantlets throughout the year each cycle requiring a short period of time. Besides propagation, this study provided an ex situ method for conservation of this vulnerable Himalayan species.D. O. I.http://dx.doi.org/10.3329/ptcb.v23i2.17506Plant Tissue Cult. & Biotech. 23(2): 147-155, 2013 (December)

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The reproductive biology of Calligonum L. in relation to ex situ conservation in a botanical garden

Archives of Biological Sciences ◽

10.2298/abs1103799x ◽

2011 ◽

Vol 63 (3) ◽

pp. 799-809 ◽

Cited By ~ 1

Author(s):

Kang Xiaoshan ◽

Pan Borong ◽

Duan Shimin ◽

Shi Wei ◽

Zhang Yongzhi

Keyword(s):

Seed Germination ◽

Ex Situ Conservation ◽

Flowering Phenology ◽

Botanical Garden ◽

Breeding Systems ◽

Ex Situ ◽

Germplasm Resources ◽

Botanic Garden ◽

Theoretical Support ◽

Germplasm Resource

In this study, we observed the flowering phenology, breeding system, pollination and seed germination of four species of Calligonum (C. calliphysa, C. rubicundum, C. densum and C. ebinuricum) in the Turpan Eremophytes Botanic Garden, China. Our results showed that the species had overlapping flowering phenologies and were pollinated by similar pollination agents. Their breeding systems were self-compatible, and with signs of outbreeding, but not of hybridization with each other; the main isolation mechanism was post-zygotic isolation and they also had high seed germination rates. Therefore, they are suited to ex situ conservation in the Turpan Eremophytes Botanic Garden, and can supply sufficient seeds for renewal populations and the conservation of germplasm resources. Furthermore, these results provide theoretical support for the construction of a national germplasm resource garden of Calligonum, and for the introduction to the garden of other eremophyteplants and their conservation.

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Hue and cry for Fritillaria cirrhosa D.Don, a threatened medicinal plant in the Western Himalaya

Discover Sustainability ◽

10.1007/s43621-021-00048-5 ◽

2021 ◽

Vol 2 (1) ◽

Author(s):

Manisha Mathela ◽

Amit Kumar ◽

Monika Sharma ◽

Gurinderjit Singh Goraya

Keyword(s):

Medicinal Plant ◽

Population Decline ◽

Plant Conservation ◽

Western Himalaya ◽

Ex Situ ◽

Conservation Strategies ◽

Market Demand ◽

Wild Populations ◽

Threatened Medicinal Plant ◽

Fritillaria Cirrhosa

AbstractThe unique Himalayan ecosystems are repositories to the wild populations of diverse flora and fauna. The high value medicinal and aromatic plant species (MAPs) are an example of the same. Since time immemorial, these MAPs have been traditionally used by the local inhabitants and have eventually developed a high market value all over the world. Increasing market demand engenders over-extraction of species, unsustainable collection further catalyses decline in wild populations. The current communication raises high conservation concern on the rapid population decline of Fritillaria cirrhosa D.Don in the Western Himalaya. Harvested and traded with a new trade name i.e., ‘Jangli lehsun’ probably to disguise common Allium species, the species is facing tremendous decline in wild populations due to its illegal harvesting and trade in Himachal Pradesh. Further, F. cirrhosa faces threat due to unorganized, over-extraction, unsustainable and premature harvesting of the bulbs, coupled with illegal hidden markets functioning parallelly. Considering that this valuable species is under multiple threats being a medicinally important plant, priority should be given for its conservation through in-situ such as identification of medicinal plant conservation areas and ex-situ methods for its propagation and multiplication. Further, to ensure the long-term conservation of Fritillaria cirrhosa, prioritized conservation strategies such as strengthening of the Biodiversity Management Committees, capacity building through awareness programs for the key stakeholders and sustainable harvesting would be the practical solution.

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Rapid in vitro selection of salt-tolerant genotypes of the potentially medicinal plant Centaurium maritimum (L.) fritsch

Archives of Biological Sciences ◽

10.2298/abs0901057m ◽

2009 ◽

Vol 61 (1) ◽

pp. 57-69 ◽

Cited By ~ 9

Author(s):

Danijela Misic ◽

B. Siler ◽

Biljana Filipovic ◽

Zorica Popovic ◽

Suzana Zivkovic ◽

...

Keyword(s):

Seed Germination ◽

Medicinal Plant ◽

Salinity Tolerance ◽

Growth Phase ◽

In Vitro Selection ◽

Growth Phases ◽

Salt Tolerant ◽

Variable Response ◽

Selection Of

We investigated differences of salinity tolerance between 'salt-tolerant' (ST) and 'salt-sensitive' (SS) genotypes of yellow centaury [Centaurium maritimum (L.) Fritsch] selected during the germination phase. The ability of in vitro cultured C. maritimum to complete the whole ontogenetic cycle in less than 6 months enabled us to deterine salinity tolerance during different growth phases. Based on the physiological attributes measured in this study (growth, morphogenesis, photosynthesis, flowering, seed germination), it can be concluded that C. maritimum genotypes differing in salinity tolerance showed a variable response to elevated salt concentrations during both the vegetative and the generative growth phase.

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