scholarly journals Morphological characters, flowering and seed germination of the Indonesian medicinal plant Orthosiphon aristatus

2019 ◽  
Vol 20 (2) ◽  
pp. 328-337 ◽  
Author(s):  
SHALATI FEBJISLAMI ◽  
ANI KURNIAWATI ◽  
MAYA MELATI ◽  
YUDIWANTI WAHYU
Keyword(s):  
Seed Germination ◽  
Medicinal Plant ◽  
Southeast Asia ◽  
Morphological Variation ◽  
Germination Rate ◽  
Leaf Shape ◽  
Morphological Characters ◽  
Ex Situ ◽  
New Information ◽  
Ex Situ Collections

Febjislami S, Kurniawati A, Melati M, Wahyu Y. 2019. Morphological characters, flowering and seed germination of the Indonesian medicinal plant Orthosiphon aristatus. Biodiversitas 20: 328-337. Orthosiphon aristatus (Blume) Miq is a popular medicinal plant in Southeast Asia. The morphological variation of O. aristatus is narrow and information on flowering and seed germination is limited. This study aimed to determine the morphological characters, flowering and seed germination of O. aristatus. The study was conducted on 19 accessions (ex situ collections) of O. aristatus from West, Central and East Java. It was found that the differences in morphological and flowering characters were mainly based on shape and color. The dominant stem color is strong yellowish green mixed with deep purplish pink in different proportions. The dominant leaf shape was medium elliptic. O. aristatus flower has three kinds of colors: purple, intermediate and white (the most common color). O. aristatus has heterostyled flower with a long-styled morph. The stigma has two shapes: open and close (the dominant shape). The open and close stigma was found in Tuban accession, the open stigma was found in Pamekasan accession. O. aristatus accessions have a narrow diversity of 84% similarity rate. O. aristatus started flowering at 4-5 weeks after planting and blooming 2-3 weeks later. O. aristatus seeds begin to germinate on the fourth day and take about eight days to grow from seedling to become complete sprouts. Count I ranged from day 5-6 and count II on days 10-11. Tuban accession has the highest germination rate of 72.97%. The results of this research can be considered as new information, regarding information about the diversity of morphological, flowering characters and the ability of O. aristatus seed to germinate.

scholarly journals FEATURES SEED REPRODUCTION OF HEDYSARUM GRANDIFLORUM PALL. EX SITU AND IN VITRO

2020 ◽  
pp. 286-293
Author(s):  
N. A. Suprun ◽  
◽  
E. V. Malaeva ◽  
S. A. Shumikhin ◽  
◽  
...  
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Natural Populations ◽  
Seed Vigor ◽  
Ex Situ ◽  
Optimal Temperature ◽  
High Productivity ◽  
Seed Reproduction ◽  
Optimal Mode

Hedysarum grandiflorum samples were collected from 7 natural populations of the Volgograd, Samara and Lugansk People's Republic and the features of seed reproduction were studied under ex situ and in vitro conditions. The optimal temperature for seed germination and the dependence of germination and seed vigor on the storage were revealed. We observed the maximum germination rate in the scarified seeds of H. grandiflorum of 2018 collection and it was equal to 70%.It was found that the seed germina-tion and seed vigor are low and quickly decrease with years of storage, while their scarification allows to increase these indicators. It is noted that the relatively low germination of the studied seeds is leveled by the rather high productivity of species in general. The optimal mode of sterilization of H. grandiflorum seeds was experimentally determined - 10% Lysofornin® 3000 solution for 5 minutes. The percentage of sterile seedlings was 60%.

scholarly journals Morphological Variation among Flowers of Bulbophyllum ovalifolium (Blume) Lindl. (Orchidaceae) in Bali

2017 ◽  
Vol 3 (4) ◽  
pp. 136
Author(s):  
Muhammad Bima Atmaja ◽  
Aninda Retno Utami Wibowo ◽  
I Gede Tirta
Keyword(s):  
Southeast Asia ◽  
Morphological Variation ◽  
Morphological Characters ◽  
High Variability ◽  
Morphological Observation ◽  
Altitude Range ◽  
Botanic Garden ◽  
Widespread Species ◽  
Orchid Species ◽  
Flowering Season

<p><em>Bulbophyllum ovalifolium</em> (Blume) Lindl., an epiphyte orchid species was collected from several region in Bali and maintained in “Eka Karya” Bali Botanic Garden. The orchid is a common and widespread species in Southeast Asia, thus makes the flower has high variability. Here a study through morphological characters on the flower was done to give more information and understandable of characteristic range. The species was found at altitude range 700 to 2000 m a.s.l., the morphological observation was conducted on 2014 to 2015 particularly in the flowering season. There were three variants of flower based on the differences on size, colour, and the lip. The character on lip surface was found as spot character with three types of surface: coarsely verrucose, scattered verrucose, and glabrous.</p><div><p class="Els-keywords"><strong>Keywords:</strong> Bali; Bulbophyllum ovalifolium; morphological variation; orchidaceae; orchidi.</p></div>

AKURASI KARAKTERISASI TINGKAT IN SITU TANAMAN UBI JALAR PADA DUA KECAMATAN DI KABUPATEN SERAM BAGIAN BARAT

2017 ◽  
Vol 13 (2) ◽  
pp. 103-110
Author(s):  
Helen Hetharie ◽  
Simon H T Raharjo ◽  
Gelora H Augustyn ◽  
Marietje Pesireron
Keyword(s):  
Sweet Potato ◽  
Ipomoea Batatas ◽  
Leaf Shape ◽  
Morphological Characters ◽  
Morphological Characterization ◽  
Ex Situ ◽  
Survey Method ◽  
In Situ Characterization

Morphological characters of above ground plant parts can be used to distinguishsweet potato accessions. The objective of this study was to get a number of diversity of sweet potato accessions based on above ground part morphology and to determine the accuracy of in situ characterization. This study used a survey method in five villages in two sub-districts, namely Inomosol and Huamual Muka, Western Ceram District. The first stage of this study involved in situ characterization, and the second stage involved planting and ex situ morphological characterization of 2-month-old plants which was used sweet potato descriptors. Data were analyzed descriptively and qualitatively. The results showed that there were 25 accessions of sweet potato found in Huamual Muka and Inomosol Sub-districts, as distinguished by leaf shape and color, leaf patterns and leaf lobe number, petiole and stem colors, and vine twisting tendency. Verification results of the above ground morphology showed accuracy of ≥80% in 3 phenotypes in leaf lobe pattern, shape of central leaf lobe, linear middle leaves, leaves with 1, 5 and 7 lobes, green abaxial leaf veins, 2 petiole color phenotypes, purplish red stem, and stem additional colors. Meanwhile, the accuracy of characterization of other phenotypes was 0-76%. Keywords: diversity, ex situ, Ipomoea batatas, Maluku, morphology   ABSTRAK Karakter morfologi tajuk ubi jalar dapat digunakan sebagai pembeda antar aksesi. Tujuan penelitian untuk mendapatkan sejumlah klon ubi jalar yang beragam berdasarkan morfologi tajuk serta ketepatan karakterisasi morfologi pada tingkat in situ. Penelitian ini menggunakan metode survei di lima desa pada dua kecamatan yaitu kecamatan Inomosol dan Huamual Muka pada Kabupaten Seram Bagian Barat, Propinsi Maluku. Tahap pertama dengan mengkarakterisasi morfologi tajuk di kebun petani (in situ), dan tahap kedua penanaman dan karakterisasi ex situ di kebun koleksi pada umur tanaman 2 bulan menggunakan deskriptor ubi jalar. Data dianalisis secara deskriptif kualitatif. Hasil penelitian menunjukkan bahwa terdapat 25 aksesi ubi jalar di kecamatan Inomosol dan Huamual Muka yang dibedakan berdasarkan bentuk dan warna daun, pola dan jumlah lekuk daun, warna tangkai dan batang, serta sifat membelit. Hasil verifikasi terhadap karakterisasi in situ didapatkan akurasi ≥80% pada 3 fenotip pada pola lekuk daun, daun dengan bentuk bagian tengah linear, daun dengan jumlah cuping 1, 5 dan 7, tulang daun permukaan bawah berwarna hijau, 2 fenotip pada warna tangkai daun, batang berwarna merah keunguan, serta ada warna tambahan pada batang. Sedangkan akurasi karakterisasi pada fenotip lainnya yaitu 0-76%. Kata kunci: ex situ, Ipomoea batatas, keragaman, Maluku, morfologi

scholarly journals Alpine Pioneer Plants in Soil Bioengineering for Slope Stabilization and Restoration: Results of a Preliminary Analysis of Seed Germination and Future Perspectives

2020 ◽  
Vol 12 (17) ◽  
pp. 7190
Author(s):  
Luca Giupponi ◽  
Valeria Leoni
Keyword(s):  
Calcium Carbonate ◽  
Seed Germination ◽  
Room Temperature ◽  
Restoration Ecology ◽  
Germination Rate ◽  
Ex Situ ◽  
Pioneer Species ◽  
Soil Bioengineering ◽  
Pioneer Plants ◽  
Dryas Octopetala

Stabilization of slopes subject to landslide by measures with low impact, such as those of bioengineering, is a topic of interest. The use of scarcely studied alpine pioneer plants could contribute to innovation in soil bioengineering and restoration ecology but to use them, knowledge of the ex situ germinability of their seeds is fundamental. This research analysed the germinability of seeds of nine alpine pioneer species (Papaver aurantiacum, Rumex scutatus, Tofieldia calyculata, Pulsatilla alpina, Silene glareosa, Adenostyles alpina, Dryas octopetala, Laserpitium peucedanoides and Laserpitium krapfii) treated with water, gibberellic acid (GA3) and/or calcium carbonate at room temperature. The seeds had different responses to the treatments: Laserpitium peucedanoides, L. krapfii and Silene glareosa showed difficulty in germinating (germination < 2.5%), while Dryas octopetala had good germination (39–61%) regardless of treatment. GA3 significantly increased the seed germination rate of Papaver aurantiacum, Pulsatilla alpina, Rumex scutatus and Tofieldia calyculata, while the addition of calcium carbonate made the seeds of Rumex scutatus and Tofieldia calyculata germinate more quickly. Results are discussed focusing on the perspectives of using alpine pioneer species in future soil bioengineering work for slopes stabilization and restoration, and on the actions that stakeholders should take to make this happen.

scholarly journals Investigation of vegetative properties and generative production of the potential ornamental and narrow endemic species Verbascum yurtkuranianum (Scrophulariaceae) for ex situ conservation

BioResources ◽  
2021 ◽  
Vol 16 (4) ◽  
pp. 7530-7549
Author(s):  
Kamil Erken
Keyword(s):  
Seed Germination ◽  
Endemic Species ◽  
Ex Situ Conservation ◽  
Germination Rate ◽  
Ultrasonic Waves ◽  
Ornamental Plant ◽  
Ex Situ ◽  
Narrow Endemic ◽  
Single Location ◽  
Number Of Individuals

Verbascum yurtkuranianum is a narrow endemic species occurring in a single location, the northern Bursa province (Turkey). It is an endangered and potentially ornamental plant. No conducted study on its life and biology, production, and aesthetic features is available. This study aimed to reveal its vegetative properties, seed characteristics, methods and requirements for seed germination, germination speed, and potential ornamental value so it can be conserved ex situ and produced. Verbascum yurtkuranianum has potential value as an ornamental plant regarding its aesthetic features as a flower. This study revealed that the total number of individuals in the species is 788. Without any treatment, 70.7% germination rate is achieved if the seeds are stored at 4 °C. The optimum germination temperature was from 15 to 20 °C (77.3% and 78.7%, respectively), and the photoperiod regulation for seed germination was 12/12 or 8/16 (light/dark) hours (74.7% and 76.0%, respectively). The most effective treatment to promote germination rate was found by implementation of 60 min ultrasonic waves (94.3%) or application of 120 min vacuum (95.3%). Germination occurred between 8 and 10 days. A parcel of ex situ conservation was constituted with the seedlings obtained from the germination studies.

scholarly journals Germination and Protocorm Formation of Ophrys Sphegodes Mill. – In Vitro Protocol for a Rare Orchid Species

2018 ◽  
Vol 67 (3-4) ◽  
pp. 196-201
Author(s):  
Jovana Dulić ◽  
Mirjana Ljubojević ◽  
Ines Prlainović ◽  
Goran Barać ◽  
Tijana Narandžić ◽  
...  
Keyword(s):  
Seed Germination ◽  
Germination Rate ◽  
Casein Hydrolysate ◽  
Habitat Destruction ◽  
Ex Situ ◽  
Organic Additives ◽  
Orchid Species ◽  
Short Period ◽  
Protocorm Formation

Summary Ophrys sphegodes Mill. is a wild orchid species which is threatened and protected due to its pollination biology, small seed and habitat destruction. The aim of this study was to establish asymbiotic germination protocol for the purpose of ex situ conservation. Two basal media Knudson C (KC) and Malmgren (MM), supplemented with organic additives (peptone (PE), L-glutamin (A)e, folic acid, casein hydrolysate (CA)) added separately and control media KC--C and MM--C were used in the present research. All the nutrition media contained 2% sucrose, 7% agar and 1% activated carbon, while their pH was adjusted to 5.8 ± 0.02 before autoclaving at 121 ° C for 20 minutes. The seeds were examined under two illumination conditions, 0/24 light/dark (L/D) and 16/8 L/D. The presented results indicate a huge influence of illumination and nutrition media on the seed germination and protocorm formation. The seed germination was overall significantly more successful in dark conditions (0/24 L/D) than with lighting (16/8 L/D). Protocorm, rhizoids and shoot formation were achieved only on the seeds cultured on MM medium, while the KC medium caused only swelling of the embryo. Organic additives had positive influence on the germination rate. According to the obtained results, the best germination rate and seedling development were achieved on MM-PE media, cultured in dark. The presented procedure accelerates the germination period and can provide a large number of plants in a relatively short period of time so it can be used for conservation programs and mass production protocol.

Ex-situ conservation of medicinal plant genetic resources managed by national agrobiodiversity center of Korea

Planta Medica ◽  
2012 ◽  
Vol 78 (11) ◽  
Author(s):  
JS Sung ◽  
CW Jeong ◽  
YY Lee ◽  
HS Lee ◽  
YA Jeon ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Genetic Resources ◽  
Ex Situ Conservation ◽  
Plant Genetic Resources ◽  
Ex Situ

scholarly journals TEMPERATURE - GERMINATION RESPONSES OF SUNFLOWER (Helianthus annuus L.) GENOTYPES / GERMINACION COMO REACCION A LA TEMPERATURA EN LOS GENOTIPOS DEL GIRASOL (Helianthus annuus L.) / GERMINATION - RÉACTION À LA TEMPÉRATURE DANS LES GÉNOTYPES DE TOURNESOL (Helianthus annuus L.)

Helia ◽  
2000 ◽  
Vol 23 (33) ◽  
pp. 97-104
Author(s):  
F.M. Khalifa ◽  
A.A. Schneiter ◽  
E.I. El Tayeb
Keyword(s):  
Seed Germination ◽  
Helianthus Annuus ◽  
Maximum Rate ◽  
Germination Rate ◽  
Thermal Time ◽  
Large Temperature ◽  
Climatic Zones ◽  
Helianthus Annuus L ◽  
Wide Range ◽  
Cardinal Temperature

SUMMARY Seed germination of six sunflower (Helianthus annuus L.) hybrids was investigated across a range of eleven constant temperatures between 5°C and 45°C. Large temperature differences in germination rate 1/t (d-1), cardinal temperature (°C) and thermal time θ (°cd) were observed among hybrids. Base temperatures (Tb) varied between 3.3°C and 6.7°C whereas maximum germination temperatures (Tm) varied between 41.7°C and 48.9°C. Final germination fraction was attained at 15°C - 25°C whereas the maximum rate of germination was attained at 30.4°C - 35.6°C. The maximum germination rate of hybrid USDA 894, the cultivar with the slowest germination rate, was only 50% of that of hybrid EX 47. The low Tb and high Tm of sunflower appear to be one of the factors which explain the successful adaptation of sunflower to a wide range of temperature. These findings are discussed in relation to the origin of the crop and its wide adaptations in diverse habitats and climatic zones.

Sign in / Sign up

Export Citation Format

Share Document