scholarly journals Vinblastine and Vincristine Production on Madagascar Periwinkle (Catharanthus roseus (L.) G. Don) Callus Culture Treated with Polethylene Glycol

2016 ◽  
Vol 20 (1) ◽  
Author(s):  
Nisa Nur Iskandar ◽  
Iriawati Iriawati
Keyword(s):  
Callus Culture ◽  
Catharanthus Roseus ◽  
Madagascar Periwinkle

scholarly journals Analysis of Several Popular Cultivars of Madagascar Periwinkle (Catharanthus roseus (L.) G. Don.) using Biochemical Markers

2013 ◽  
Vol 5 (4) ◽  
pp. 458-461
Author(s):  
Owk ANIEL KUMAR ◽  
Sape S. TATA ◽  
Kancharla PAVAN KUMAR
Keyword(s):  
Catharanthus Roseus ◽  
Gel Electrophoresis ◽  
Biochemical Markers ◽  
Polyacrylamide Gel Electrophoresis ◽  
Cultivar Differences ◽  
Madagascar Periwinkle ◽  
Large White ◽  
Electrophoretic Patterns ◽  
Native Polyacrylamide Gel Electrophoresis ◽  
Isozyme Polymorphism

Band designs of esterase (EST), peroxidase (PO) and polyphenol oxidase (PPO) isozymes in several selected cultivars of Catharanthus roseus by using native polyacrylamide gel electrophoresis (PAGE) were investigated in this study. It was confirmed that cultivar differences in isozyme polymorphism can be revealed by applied electrophoretic patterns. Three isozyme systems produced a total of 16 bands with polymorphism ranged from 66.6-100%. Considering the patterns of isozyme variations in the five cultivars of Catharanthus roseus, it is evident that the cultivar ‘First kiss coral’ displayed crimson red petal with large white eye’ displayed demarked profiles of EST, PO and PPO isozymes than other cultivars. This is the first report on isozyme polymorphism in members of the Cathanarathus roseus (L.) G. Don.

Development of STMS markers from the medicinal plant Madagascar periwinkle [Catharanthus roseus (L.) G. Don.]

2005 ◽  
Vol 5 (4) ◽  
pp. 818-820 ◽  
Author(s):  
BHUMIKA SHOKEEN ◽  
NIROJ K. SETHY ◽  
SHALU CHOUDHARY ◽  
SABHYATA BHATIA
Keyword(s):  
Medicinal Plant ◽  
Catharanthus Roseus ◽  
Madagascar Periwinkle ◽  
Stms Markers

scholarly journals The bHLH transcription factor BIS1 controls the iridoid branch of the monoterpenoid indole alkaloid pathway in Catharanthus roseus

2015 ◽  
Vol 112 (26) ◽  
pp. 8130-8135 ◽  
Author(s):  
Alex Van Moerkercke ◽  
Priscille Steensma ◽  
Fabian Schweizer ◽  
Jacob Pollier ◽  
Ivo Gariboldi ◽  
...  
Keyword(s):  
Transcription Factor ◽  
Catharanthus Roseus ◽  
Transcriptional Activation ◽  
Indole Alkaloid ◽  
Bioactive Metabolites ◽  
Bhlh Transcription Factor ◽  
Ethylene Response Factor ◽  
Madagascar Periwinkle ◽  
Genes Encoding ◽  
Monoterpenoid Indole Alkaloid

Plants make specialized bioactive metabolites to defend themselves against attackers. The conserved control mechanisms are based on transcriptional activation of the respective plant species-specific biosynthetic pathways by the phytohormone jasmonate. Knowledge of the transcription factors involved, particularly in terpenoid biosynthesis, remains fragmentary. By transcriptome analysis and functional screens in the medicinal plant Catharanthus roseus (Madagascar periwinkle), the unique source of the monoterpenoid indole alkaloid (MIA)-type anticancer drugs vincristine and vinblastine, we identified a jasmonate-regulated basic helix–loop–helix (bHLH) transcription factor from clade IVa inducing the monoterpenoid branch of the MIA pathway. The bHLH iridoid synthesis 1 (BIS1) transcription factor transactivated the expression of all of the genes encoding the enzymes that catalyze the sequential conversion of the ubiquitous terpenoid precursor geranyl diphosphate to the iridoid loganic acid. BIS1 acted in a complementary manner to the previously characterized ethylene response factor Octadecanoid derivative-Responsive Catharanthus APETALA2-domain 3 (ORCA3) that transactivates the expression of several genes encoding the enzymes catalyzing the conversion of loganic acid to the downstream MIAs. In contrast to ORCA3, overexpression of BIS1 was sufficient to boost production of high-value iridoids and MIAs in C. roseus suspension cell cultures. Hence, BIS1 might be a metabolic engineering tool to produce sustainably high-value MIAs in C. roseus plants or cultures.

scholarly journals Expressed sequence tags from Madagascar periwinkle (Catharanthus roseus)

FEBS Letters ◽  
2006 ◽  
Vol 580 (18) ◽  
pp. 4501-4507 ◽  
Author(s):  
Jun Murata ◽  
Dorothee Bienzle ◽  
Jim E. Brandle ◽  
Christoph W. Sensen ◽  
Vincenzo De Luca
Keyword(s):  
Catharanthus Roseus ◽  
Expressed Sequence Tags ◽  
Madagascar Periwinkle ◽  
Expressed Sequence

Colonization of Madagascar periwinkle (Catharanthus roseus), by endophytes encoding gfp marker

2013 ◽  
Vol 195 (7) ◽  
pp. 483-489 ◽  
Author(s):  
Adalgisa Ribeiro Torres ◽  
Welington Luiz Araújo ◽  
Luciana Cursino ◽  
Priscilla de Barros Rossetto ◽  
Mateus Mondin ◽  
...  
Keyword(s):  
Catharanthus Roseus ◽  
Madagascar Periwinkle

Missing enzymes in the biosynthesis of the anticancer drug vinblastine in Madagascar periwinkle

Science ◽  
2018 ◽  
Vol 360 (6394) ◽  
pp. 1235-1239 ◽  
Author(s):  
Lorenzo Caputi ◽  
Jakob Franke ◽  
Scott C. Farrow ◽  
Khoa Chung ◽  
Richard M. E. Payne ◽  
...  
Keyword(s):  
Bioactive Compounds ◽  
Anticancer Drug ◽  
Catharanthus Roseus ◽  
Chemical Diversity ◽  
Additional Source ◽  
Madagascar Periwinkle ◽  
Biosynthetic Genes

Vinblastine, a potent anticancer drug, is produced byCatharanthus roseus(Madagascar periwinkle) in small quantities, and heterologous reconstitution of vinblastine biosynthesis could provide an additional source of this drug. However, the chemistry underlying vinblastine synthesis makes identification of the biosynthetic genes challenging. Here we identify the two missing enzymes necessary for vinblastine biosynthesis in this plant: an oxidase and a reductase that isomerize stemmadenine acetate into dihydroprecondylocarpine acetate, which is then deacetoxylated and cyclized to either catharanthine or tabersonine via two hydrolases characterized herein. The pathways show how plants create chemical diversity and also enable development of heterologous platforms for generation of stemmadenine-derived bioactive compounds.

scholarly journals Catharanthus roseus, an Experimental Host Plant for the Citrus Strain of Xylella fastidiosa

Plant Disease ◽  
2001 ◽  
Vol 85 (3) ◽  
pp. 246-251 ◽  
Author(s):  
P. B. Monteiro ◽  
J. Renaudin ◽  
S. Jagoueix-Eveillard ◽  
A. J. Ayres ◽  
M. Garnier ◽  
...  
Keyword(s):  
Catharanthus Roseus ◽  
Xylella Fastidiosa ◽  
Post Inoculation ◽  
Herbaceous Plant ◽  
Protein Subunit ◽  
Madagascar Periwinkle ◽  
Experimental Host ◽  
Leaf Chlorosis ◽  
Pathogenicity Tests ◽  
Leaf Deformation

We verified by pathogenicity tests that the herbaceous plant Catharanthus roseus (Madagascar periwinkle) can be used as an experimental host for the strain of Xylella fastidiosa that causes citrus variegated chlorosis (CVC). Plants were mechanically inoculated with CVC strain 9a5c, the genome of which was recently sequenced. Plants were inoculated with the virulent 8th passage (9a5c-8) and the 51st passage (9a5c-51). Leaf deformation and stunting were seen 2 months after inoculation on 18 of 21 plants with 9a5c-8 and 8 of 21 plants with 9a5c-51. The plants were infected with X. fastidiosa as shown by polymerase chain reaction. The bacterium could be reisolated from all plants tested, showing that CVC-X. fastidiosa multiplied and moved systemically in C. roseus plants causing dysfunction in plant growth. The disease symptoms evolved within 4 months post-inoculation to a severe leaf chlorosis in all inoculated plants. The localization of X. fastidiosa in the xylem was verified by immunofluorescence. Genes coding for proteins with homologies to plant sterol-C-methyltransferase, a transketolase-like protein, subunit III of photosystem I, and a desiccation protectant protein were found to be differentially expressed in symptomatic C. roseus plants as a response to infection with X. fastidiosa in comparison to healthy plants. A tentative correlation between the pattern of expression of these C. roseus genes with the mechanism of pathogenicity of X. fastidiosa is discussed.

Complete nucleotide sequences of two begomoviruses infecting Madagascar periwinkle (Catharanthus roseus) from Pakistan

2012 ◽  
Vol 158 (2) ◽  
pp. 505-510 ◽  
Author(s):  
Muhammad Ilyas ◽  
Kiran Nawaz ◽  
Muhammad Shafiq ◽  
Muhammad Saleem Haider ◽  
Ahmad Ali Shahid
Keyword(s):  
Catharanthus Roseus ◽  
Nucleotide Sequences ◽  
Madagascar Periwinkle

scholarly journals First Report of Sclerotium rolfsii on Catharanthus roseus

Plant Disease ◽  
2000 ◽  
Vol 84 (2) ◽  
pp. 200-200
Author(s):  
G. E. Holcomb
Keyword(s):  
Catharanthus Roseus ◽  
Fungal Pathogen ◽  
Baton Rouge ◽  
Sclerotium Rolfsii ◽  
The United States ◽  
Madagascar Periwinkle ◽  
Basal Stem Rot ◽  
First Report ◽  
Pathogenicity Tests ◽  
Stem Necrosis

Wilt, blight, and stem necrosis were observed on Catharanthus roseus (L.) G. Don ‘Mediterranean Deep Rose’ (MDR) plants (Madagascar or rose periwinkle) in August 1999 at Burden Research Plantation in Baton Rouge, LA. MDR was the only prostrate-form cultivar and the only cultivar of 11 that was diseased. Twelve of twenty-four plants of cv. MDR were killed in the trial planting. White mycelia and small (1 mm diameter) light brown sclerotia were present at the base of infected plants. The suspect fungus was isolated consistently on acidified water agar and maintained on acidified potato dextrose agar (APDA). Pathogenicity tests were done by pipetting 1 ml of blended inoculum (contents of one 7-day-old plate culture grown on APDA in 100 ml of deionized water) at the base of nine 15-cm-tall Madagascar periwinkle plants. Inoculated and noninoculated plants were held in a dew chamber for 3 days at 28°C and placed in a greenhouse where temperatures ranged between 25 and 31°C. All inoculated plants showed wilt, blight, and basal stem rot after 3 days and were dead after 10 days. Noninoculated plants remained symptomless. The fungal pathogen was identified as Sclerotium rolfsii Sacc. and was reisolated from inoculated plants. The fungus was previously reported on Lochnera rosea (L.) Rchb. (=C. roseus) from Taiwan (1). This is the first report of the occurrence of S. rolfsii on Madagascar periwinkle in the United States. Reference: (1) K. Goto. Trans. Nat. Hist. Soc. Formosa 23:37, 1933.

scholarly journals Terpenoid indole alkaloid biosynthesis in Catharanthus roseus: effects and prospects of environmental factors in metabolic engineering

2021 ◽  
Vol 43 (11) ◽  
pp. 2085-2103
Author(s):  
Yongliang Liu ◽  
Barunava Patra ◽  
Sanjay Kumar Singh ◽  
Priyanka Paul ◽  
Yan Zhou ◽  
...  
Keyword(s):  
Gene Expression ◽  
Metabolic Engineering ◽  
Environmental Factors ◽  
Catharanthus Roseus ◽  
Abiotic Factors ◽  
Indole Alkaloids ◽  
Indole Alkaloid ◽  
Terpenoid Indole Alkaloids ◽  
Madagascar Periwinkle ◽  
Specialized Metabolites

Abstract Plants synthesize a vast array of specialized metabolites that primarily contribute to their defense and survival under adverse conditions. Many of the specialized metabolites have therapeutic values as drugs. Biosynthesis of specialized metabolites is affected by environmental factors including light, temperature, drought, salinity, and nutrients, as well as pathogens and insects. These environmental factors trigger a myriad of changes in gene expression at the transcriptional and posttranscriptional levels. The dynamic changes in gene expression are mediated by several regulatory proteins that perceive and transduce the signals, leading to up- or down-regulation of the metabolic pathways. Exploring the environmental effects and related signal cascades is a strategy in metabolic engineering to produce valuable specialized metabolites. However, mechanistic studies on environmental factors affecting specialized metabolism are limited. The medicinal plant Catharanthus roseus (Madagascar periwinkle) is an important source of bioactive terpenoid indole alkaloids (TIAs), including the anticancer therapeutics vinblastine and vincristine. The emerging picture shows that various environmental factors significantly alter TIA accumulation by affecting the expression of regulatory and enzyme-encoding genes in the pathway. Compared to our understanding of the TIA pathway in response to the phytohormone jasmonate, the impacts of environmental factors on TIA biosynthesis are insufficiently studied and discussed. This review thus focuses on these aspects and discusses possible strategies for metabolic engineering of TIA biosynthesis. Purpose of work Catharanthus roseus is a rich source of bioactive terpenoid indole alkaloids (TIAs). The objective of this work is to present a comprehensive account of the influence of various biotic and abiotic factors on TIA biosynthesis and to discuss possible strategies to enhance TIA production through metabolic engineering.

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