Neutron phase contrast tomographic imaging method

Abstract Objectives This article shows an imaging method of the stomach that does not use imaging agents. X-ray phase-contrast images of different stages of gastric development were taken using X-ray in-line phase-contrast imaging (XILPCI). The aim of the study was to demonstrate that XILPCI is a micron imaging method for gastric structures. Methods The stomachs of 4-, 6- and 12-week-old rats were removed and cleaned. XILPCI has 1000 times greater soft tissue contrast than that of X-ray traditional absorption radiography. The projection images of the rats’ stomachs were recorded by an XILPCI charge coupled device (CCD) at 9 μm image resolution. Results The X-ray in-line phase-contrast images of the different stages of rat gastric specimens clearly showed the gastric architectures and the details of the gastroduodenal region. 3-dimensional stomach anatomical structure images were reconstruction. Conclusion The reconstructed gastric 3D images can clearly display the internal structure of the stomach. XILPCI may be a useful method for medical research in the future. Keywords: Synchrotron radiation phase-contrast imaging, 3-dimensional gastric structure images

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Amyloid-β plaque deposition measured using propagation-based X-ray phase contrast CT imaging

Journal of Synchrotron Radiation ◽

10.1107/s1600577516004045 ◽

2016 ◽

Vol 23 (3) ◽

pp. 813-819 ◽

Cited By ~ 10

Author(s):

Alberto Astolfo ◽

Aurélien Lathuilière ◽

Vanessa Laversenne ◽

Bernard Schneider ◽

Marco Stampanoni

Keyword(s):

Spatial Resolution ◽

Phase Contrast ◽

Ex Vivo ◽

Three Dimensional ◽

Amyloid Β ◽

High Sensitivity ◽

Final Diagnosis ◽

Pathological Process ◽

Imaging Method ◽

X Ray

Amyloid beta accumulation into insoluble plaques (Aβp) is known to play a significant role in the pathological process in Alzheimer's disease (AD). The presence of Aβp is also one of the neuropathological hallmarks for the disease. AD final diagnosis is generally acknowledged after the evaluation of Aβp deposition in the brain. Insoluble Aβp accumulation may also concur to cause AD as postulated in the so-calledamyloid hypothesis. Therefore, the visualization, evaluation and quantification of Aβp are nowadays the keys for a better understanding of the disease, which may point to a possible cure for AD in the near future. Synchrotron-based X-ray phase contrast (XPC) has been demonstrated as the only imaging method that can retrieve the Aβp signal with high spatial resolution (up to 10 µm), high sensitivity and three-dimensional information at the same time. Although at the moment XPC is suitable forex vivosamples only, it may develop into an alternative to positron emission tomography and magnetic resonance imaging in Aβp imaging. In this contribution the possibility of using synchrotron-based X-ray phase propagation computed tomography to visualize and measure Aβp on mouse brains is presented. A careful setup optimization for this application leads to a significant improvement of spatial resolution (∼1 µm), data acquisition speed (five times faster), X-ray dose (five times lower) and setup complexity, without a substantial loss in sensitivity when compared with the classic implementation of grating-based X-ray interferometry.

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Direct information retrieval after 3D reconstruction in grating-based X-ray phase-contrast computed tomography

Journal of Synchrotron Radiation ◽

10.1107/s1600577518008019 ◽

2018 ◽

Vol 25 (4) ◽

pp. 1222-1228 ◽

Cited By ~ 1

Author(s):

Zhao Wu ◽

Kun Gao ◽

Zhili Wang ◽

Chenxi Wei ◽

Faiz Wali ◽

...

Keyword(s):

Computed Tomography ◽

Information Retrieval ◽

Phase Contrast ◽

Three Dimensional ◽

Large Field ◽

Computational Time ◽

Imaging Method ◽

Phase Reconstruction ◽

Phase Information ◽

X Ray

Grating-based X-ray differential phase-contrast imaging has attracted a great amount of attention and has been considered as a potential imaging method in clinical medicine because of its compatibility with the traditional X-ray tube source and the possibility of a large field of view. Moreover, phase-contrast computed tomography provides three-dimensional phase-contrast visualization. Generally, two-dimensional information retrieval performed on every projection is required prior to three-dimensional reconstruction in phase-contrast computed tomography. In this paper, a three-dimensional information retrieval method to separate absorption and phase information directly from two reconstructed images is derived. Theoretical derivations together with numerical simulations have been performed to confirm the feasibility and veracity of the proposed method. The advantages and limitations compared with the reverse projection method are also discussed. Owing to the reduced data size and the absence of a logarithm operation, the computational time for information retrieval is shortened by the proposed method. In addition, the hybrid three-dimensional images of absorption and phase information were reconstructed using an absorption reconstruction algorithm, hence the existing data pre-processing methods and iterative reconstruction algorithms in absorption reconstruction may be utilized in phase reconstruction immediately.

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Pseudocoherent phase-contrast imaging method with variable focus depth: an effective tool for low-contrast radiological image analysis

10.1117/12.310967 ◽

1998 ◽

Cited By ~ 1

Author(s):

Alexander M. Akhmetshin

Keyword(s):

Image Analysis ◽

Phase Contrast ◽

Phase Contrast Imaging ◽

Imaging Method ◽

Contrast Imaging ◽

Low Contrast ◽

Focus Depth ◽

Radiological Image ◽

Variable Focus

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Tomographic reconstruction using tilted Laue analyser-based X-ray phase-contrast imaging

Journal of Synchrotron Radiation ◽

10.1107/s1600577520013995 ◽

2021 ◽

Vol 28 (1) ◽

pp. 283-291

Author(s):

M. C. Chalmers ◽

M. J. Kitchen ◽

K. Uesugi ◽

G. Falzon ◽

P. Quin ◽

...

Keyword(s):

Phase Contrast ◽

Tomographic Reconstruction ◽

Phase Contrast Imaging ◽

Imaging Method ◽

Contrast Imaging ◽

Phase Gradient ◽

X Ray ◽

Sensitive Phase ◽

Phase Contrast Method ◽

Phase Sensitive

Analyser-based phase-contrast imaging (ABPCI) is a highly sensitive phase-contrast imaging method that produces high-contrast images of weakly absorbing materials. However, it is only sensitive to phase gradient components lying in the diffraction plane of the analyser crystal [i.e. in one dimension (1-D)]. In order to accurately account for and measure phase effects produced by the wavefield-sample interaction, ABPCI and other 1-D phase-sensitive methods must achieve 2-D phase gradient sensitivity. An inclined geometry method was applied to a Laue geometry setup for X-ray ABPCI through rotation of the detector and object about the optical axis. This allowed this traditionally 1-D phase-sensitive phase-contrast method to possess 2-D phase gradient sensitivity. Tomographic datasets were acquired over 360° of a multi-material phantom with the detector and sample tilted by 8°. The real and imaginary parts of the refractive index were reconstructed for the phantom.

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Three-dimensional Construction of Micrometer Level in Rat Stomach by Synchrotron Radiation

10.21203/rs.3.rs-38915/v2 ◽

2020 ◽

Author(s):

Qiang Tao ◽

Chen-Chen Gao ◽

Xue-Hong Tong ◽

Shi-Zhen Yuan ◽

Jingdong Xu

Keyword(s):

Synchrotron Radiation ◽

Phase Contrast ◽

Structural Changes ◽

Three Dimensional ◽

Image Resolution ◽

Imaging Method ◽

Contrast Imaging ◽

X Ray ◽

3 Dimensional ◽

Tissue Contrast

Abstract Background: The structural changes of gastric mucosa are considered as an important window of early gastric lesions. This article shows an imaging method of the stomach that does not use imaging agents. X-ray phase-contrast images of different stages of gastric development were taken using micrometer level X-ray in-line phase-contrast imaging (XILPCI) technique on synchrotron radiation facility. The aim of the study was to demonstrate that the imaging technique is an appropriate method for micron imaging of the gastric structures.Methods: The stomachs of 4-, 6- and 12-week-old rats were removed and cleaned. XILPCI has 1000 times greater soft tissue contrast than that of X-ray traditional absorption radiography. The projection images of the rats stomachs were recorded by an XILPCI charge coupled device (CCD) at 9 μm image resolution.Results: The X-ray in-line phase-contrast images of the different stages of rats’ gastric specimens clearly showed the gastric architectures and the details of the gastro-duodenal region. 3-dimensional stomach anatomical structure images were reconstruction. Conclusion: The reconstructed gastric 3D images can clearly display the internal structure of the stomach. XILPCI may be a useful method for medical research in the future.

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