scholarly journals Caracterización epidemiológica de casos humanos compatibles con rickettsiosis que consultaron a instituciones de salud del departamento de Caldas-Colombia, años 2016-2019

Biomédica ◽  
2021 ◽  
Vol 41 (Sp. 2) ◽  
Author(s):  
Jorge Enrique Pérez ◽  
Gloria Inés Estrada ◽  
Yuliana Zapata ◽  
Marylin Hidalgo ◽  
Cristian Camilo Serna ◽  
...  
Keyword(s):  
Rickettsia Typhi ◽  
Rickettsia Felis ◽  
Rickettsia Rickettsii

Introducción: Las rickettsiosis son enfermedades zoonóticas, algunos artrópodos cumplen el papel de vectores. la inespecificidad de los síntomas hace que su diagnóstico clínico sea difícil. La Inmunofluorescencia indirecta (IFI), se usa en el diagnóstico. En Colombia, a partir del 2003, ha resurgido el interés por realizar estudios en búsqueda de dicha patología. Objetivo: Caracterizar epidemiológicamente los casos humanos compatibles con rickettsiosis que consultan a las instituciones de salud del departamento de Caldas-Colombia, durante el periodo de tiempo comprendido en los años 2016- 2019. Métodos: Se realizó un estudio de diseño cuantitativo, observacional, descriptivo, con una muestra no probabilística constituida por 175 pacientes con síntomas compatibles con rickettsiosis que consultaron en diferentes municipios de Caldas- Colombia; se les realizo IFI para la detección de anticuerpos en fase aguda y convaleciente frente a Rickettsia rickettsii, Rickettsia typhi y Rickettsia felis. Resultados: El promedio de edad fue de 31 años, Los municipios con mayor proporción de casos son Belalcázar, Chinchiná, Filadelfia, La Dorada, La Merced, Manizales. El 66 % tenían mascotas, frecuentemente perros; el 12% manifiestan picaduras por artrópodos. Los signos y síntomas más frecuentes fueron: cefalea 69,7%, artromialgia 60%, fiebre 58,2%. La seroprevalencia por IgG fue del 60%, 47,9% y 24% para R. rickettsii, R. typhi y R. felis respectivamente. Cinco pacientes presentaron seroconversión frente a R. rickettsii y R. felis y uno frente a R. typhi. Conclusión: Hay evidencia de enfermedad rickettsial en el departamento, predominantemente asociada con rickettsias del grupo de las fiebres manchadas.

scholarly journals Rickettsia typhi IN RODENTS AND R. felis IN FLEAS IN YUCATÁN AS A POSSIBLE CAUSAL AGENT OF UNDEFINED FEBRILE CASES

2015 ◽  
Vol 57 (2) ◽  
pp. 129-132 ◽  
Author(s):  
Gaspar PENICHE-LARA ◽  
Karla DZUL-ROSADO ◽  
Carlos PÉREZ-OSORIO ◽  
Jorge ZAVALA-CASTRO
Keyword(s):  
Rattus Rattus ◽  
Conventional Polymerase Chain Reaction ◽  
Causal Agent ◽  
Wild Rodents ◽  
Murine Typhus ◽  
Rickettsia Typhi ◽  
Rickettsia Felis ◽  
Vector Borne ◽  
Polymerase Chain ◽  
First Time

Rickettsia typhi is the causal agent of murine typhus; a worldwide zoonotic and vector-borne infectious disease, commonly associated with the presence of domestic and wild rodents. Human cases of murine typhus in the state of Yucatán are frequent. However, there is no evidence of the presence of Rickettsia typhi in mammals or vectors in Yucatán. The presence of Rickettsia in rodents and their ectoparasites was evaluated in a small municipality of Yucatán using the conventional polymerase chain reaction technique and sequencing. The study only identified the presence of Rickettsia typhi in blood samples obtained from Rattus rattus and it reported, for the first time, the presence of R. felis in the flea Polygenis odiosus collected from Ototylomys phyllotis rodent. Additionally, Rickettsia felis was detected in the ectoparasite Ctenocephalides felis fleas parasitizing the wild rodent Peromyscus yucatanicus. This study’s results contributed to a better knowledge of Rickettsia epidemiology in Yucatán.

Shell-vial culture and real-time PCR applied to Rickettsia typhi and Rickettsia felis detection

2015 ◽  
Vol 31 (11) ◽  
pp. 1747-1754 ◽  
Author(s):  
Ferran Segura ◽  
Immaculada Pons ◽  
Júlia Pla ◽  
María-Mercedes Nogueras
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Rickettsia Typhi ◽  
Rickettsia Felis ◽  
Shell Vial

Seroepidemiological study of Rickettsia felis, Rickettsia typhi, and Rickettsia conorii infection among the population of southern Spain

2006 ◽  
Vol 25 (6) ◽  
pp. 375-381 ◽  
Author(s):  
M. Bernabeu-Wittel ◽  
M. D. Toro ◽  
M. M. Nogueras ◽  
M. A. Muniain ◽  
N. Cardeñosa ◽  
...  
Keyword(s):  
Southern Spain ◽  
Rickettsia Conorii ◽  
Rickettsia Typhi ◽  
Rickettsia Felis

scholarly journals Molecular and Functional Analysis of the lepB Gene, Encoding a Type I Signal Peptidase from Rickettsia rickettsii and Rickettsia typhi

2003 ◽  
Vol 185 (15) ◽  
pp. 4578-4584 ◽  
Author(s):  
M. Sayeedur Rahman ◽  
Jason A. Simser ◽  
Kevin R. Macaluso ◽  
Abdu F. Azad
Keyword(s):  
Spotted Fever ◽  
Rocky Mountain ◽  
Amino Acid Sequences ◽  
Signal Peptidase ◽  
Type I ◽  
Rocky Mountain Spotted Fever ◽  
Rickettsia Typhi ◽  
Rickettsia Rickettsii ◽  
Signal Peptidase I

ABSTRACT The type I signal peptidase lepB genes from Rickettsia rickettsii and Rickettsia typhi, the etiologic agents of Rocky Mountain spotted fever and murine typhus, respectively, were cloned and characterized. Sequence analysis of the cloned lepB genes from R. rickettsii and R. typhi shows open reading frames of 801 and 795 nucleotides, respectively. Alignment analysis of the deduced amino acid sequences reveals the presence of highly conserved motifs that are important for the catalytic activity of bacterial type I signal peptidase. Reverse transcription-PCR and Northern blot analysis demonstrated that the lepB gene of R. rickettsii is cotranscribed in a polycistronic message with the putative nuoF (encoding NADH dehydrogenase I chain F), secF (encoding protein export membrane protein), and rnc (encoding RNase III) genes in a secF-nuoF-lepB-rnc cluster. The cloned lepB genes from R. rickettsii and R. typhi have been demonstrated to possess signal peptidase I activity in Escherichia coli preprotein processing in vivo by complementation assay.

Prevalence of Rickettsia species antibodies and Rickettsia species DNA in the blood of cats with and without fever

2009 ◽  
Vol 11 (4) ◽  
pp. 266-270 ◽  
Author(s):  
Danielle B. Bayliss ◽  
Arianne K. Morris ◽  
Mauricio C. Horta ◽  
Marcelo B. Labruna ◽  
Steven V. Radecki ◽  
...  
Keyword(s):  
Body Temperature ◽  
Deoxyribonucleic Acid ◽  
Matched Control ◽  
Clinical Signs ◽  
Rickettsial Infection ◽  
Rickettsia Felis ◽  
Rickettsia Species ◽  
Rickettsia Rickettsii ◽  
Paired Samples ◽  
Polymerase Chain

Rickettsia species antibodies have been detected in some cats but it is unknown whether infected cats develop clinical signs. The prevalence of Rickettsia species deoxyribonucleic acid (DNA) in blood from clinically ill cats has not been determined. The objective of this study was to determine if cats with fever (body temperature ≥102.5°F [39.2°C]) were more likely to have evidence of rickettsial infection than healthy, age-matched, control cats with a body temperature<102.5°F. Rickettsia species polymerase chain reaction (PCR) assays were performed to detect rickettsial DNA extracted from blood (71 paired samples), indirect immunofluorescence assays (IFA) were performed to detect serum antibodies against Rickettsia felis (90 paired samples) and Rickettsia rickettsii (91 paired samples), and the results between pairs were compared. All samples were negative for Rickettsia species DNA. More cats with fever were seropositive for R felis or R rickettsii than control cats, but results were not statistically significant. Results of this pilot study failed to show an association between Rickettsia species DNA or Rickettsia species antibodies and fever.

Fleas of Shrews and Rodents in Rural Lowland Taiwan

2019 ◽  
Author(s):  
Hsi-Chieh Wang ◽  
Pei-Lung Lee ◽  
Chi-Chien Kuo
Keyword(s):  
Human Case ◽  
Rickettsia Conorii ◽  
Mammal Species ◽  
Rickettsia Felis ◽  
Closely Related Species ◽  
Main Island ◽  
Xenopsylla Cheopis ◽  
Rickettsia Rickettsii ◽  
Southern Taiwan ◽  
Small Mammal Species

Abstract Fleas transmit a variety of pathogens to humans but are relatively understudied in comparison to mosquitoes and ticks, including in Taiwan, where fleas in rural lowlands have never been systematically surveyed. In total, 700 fleas of four species were collected from 1,260 shrews and rodents at nine counties across lowland Taiwan. Nosopsyllus nicanus Jordan (Siphonaptera: Ceratophyllidae) and Xenopsylla cheopis Rothschild (Siphonaptera: Pulicidae) were the most abundant flea species (79.0 and 14.6% of total fleas, respectively); the former was largely limited to the islets, while the latter was restricted to the Taiwan main island. Rattus losea Swinhoe (Rodentia: Muridae) was the most common small mammal species (49.3% of total) and hosted the majority of fleas (88.3% of total). Five Rickettsia spp., including Rickettsia conorii Brumpt (Rickettsiales: Rickettsiaceae), Rickettsia felis Bouyer et al. Rickettsia japonica Uchida, Rickettsia raoultii Mediannikov, and Rickettsia rickettsii Brumpt or closely related species, were identified from 67 individually assayed fleas based on ompB and gltA genes. Rickettsia felis, mainly transmitted by fleas, was detected in one X. cheopis in southern Taiwan where a confirmed human case of infection with R. felis has been reported. The presence of R. felis, along with the other four tick-borne Rickettsia spp., demonstrates that a variety of rickettsiae circulate in rural lowland Taiwan and could pose risks to human health.

Evidence of Infection in Humans with Rickettsia typhi and Rickettsia felis in Catalonia in the Northeast of Spain

2006 ◽  
Vol 1078 (1) ◽  
pp. 159-161 ◽  
Author(s):  
M. M. NOGUERAS ◽  
N. CARDENOSA ◽  
I. SANFELIU ◽  
T. MUNOZ ◽  
B. FONT ◽  
...  
Keyword(s):  
Rickettsia Typhi ◽  
Rickettsia Felis
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