scholarly journals Cryptic diversity in the subgenus Oxyphortica (Diptera, Drosophilidae, Stegana)

PeerJ ◽  
2021 ◽  
Vol 9 ◽  
pp. e12347
Author(s):  
Ya-Lian Wang ◽  
Nan-Nan Wang ◽  
Yuan Zhang ◽  
Shun-Chern Tsaur ◽  
Hong-Wei Chen
Keyword(s):  
New Species ◽  
Species Delimitation ◽  
Southwest China ◽  
Morphological Characteristics ◽  
Mitochondrial Genes ◽  
Species Boundaries ◽  
Molecular Evidence ◽  
Hengduan Mountains ◽  
Cryptic Diversity ◽  
Barcode Gap

Phylogenetic relationships of the subgenus Oxyphortica were reconstructed based on two mitochondrial genes (COI and ND2). The results revealed the paraphyly of Oxyphortica and supported high levels of cryptic diversity within this subgenus. By integrating morphological characteristics and molecular evidence, we identified 17 new species as members of Oxyphortica: S. (O.) amphigya sp. nov., S. (O.) armillata sp. nov., S. (O.) ashima sp. nov., S. (O.) bawo sp. nov., S. (O.) crypta sp. nov., S. (O.) gelea sp. nov., S. (O.) hengduanmontana sp. nov., S. (O.) jinmingi sp. nov., S. (O.) mengbalanaxi sp. nov., S. (O.) mouig sp. nov., S. (O.) setipes sp. nov., S. (O.) shangrila sp. nov., S. (O.) tsauri sp. nov., S. (O.) valleculata sp. nov., S. (O.) wanhei sp. nov., S. (O.) yangjin sp. nov. and S. (O.) hypophaia sp. nov. To test the early morphological identifications and confirm the species boundaries, different species delimitation methods, including Automatic Barcode Gap Discovery (ABGD) and Bayesian Phylogenetics and Phylogeography (BP&P), were used, together with traditional distance. All species boundaries were clearly defined. As Oxyphortica species are mainly distributed across Southwest China (e.g., 20 spp. from the Hengduan Mountains), the complex climate and topographic landforms of the area may be responsible for the high levels of species diversity and endemism.

Molecular phylogeny and species delimitation of Amiota alboguttata and Amiota basdeni species groups (Diptera: Drosophilidae) from East Asia

2020 ◽  
Vol 189 (4) ◽  
pp. 1370-1397 ◽  
Author(s):  
Ya-Lian Wang ◽  
Hui-Luo Cao ◽  
Hong-Wei Chen
Keyword(s):  
New Species ◽  
Molecular Phylogeny ◽  
East Asia ◽  
Species Delimitation ◽  
Mitochondrial Gene ◽  
Molecular Evidence ◽  
Centre Of Origin ◽  
Species Groups ◽  
Three New Species ◽  
Barcode Gap

Abstract Phylogenetic relationships of the two largest species groups in the genus Amiota [the Amiota alboguttata group (29 known and six new species) and the Amiota basdeni group (17 known and three new species)] were reconstructed using two mitochondrial gene sequences (COI and ND2). The paraphyly of the A. alboguttata group was identified based on molecular evidence. The monophyletic A. basdeni group is found to be nested in the A. alboguttata group, rendering the latter paraphyletic. Automatic barcode gap discovery and Bayesian phylogenetics and phylogeography methods were used to assess species limits of the A. alboguttata and A. basdeni groups. The results indicate that most analysed species could be delimited clearly, including nine new species (Amiota beama sp. nov., Amiota cyclophylla sp. nov., Amiota flormontana sp. nov., Amiota obtusa sp. nov., Amiota planiceps sp. nov., Amiota scrobicula sp. nov., Amiota jianjuni sp. nov., Amiota tentacula sp. nov. and Amiota xinglaii sp. nov.), except for some closely related morphospecies. Furthermore, based on the highly biased distributions of these two groups, southwestern China is hypothesized to be a possible centre of origin and diversification for Amiota in East Asia.

scholarly journals Integrative taxonomy of the genus Pseudostegana (Diptera, Drosophilidae) from China, with descriptions of eleven new species

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5160 ◽  
Author(s):  
Jinming Lu ◽  
Yuan Zhang ◽  
Hongwei Chen
Keyword(s):  
New Species ◽  
Species Delimitation ◽  
Morphological Characteristics ◽  
Morphological Characters ◽  
Molecular Data ◽  
Integrative Taxonomy ◽  
Southwestern China ◽  
28S Rrna ◽  
Nuclear Locus ◽  
Barcode Gap

The genus Pseudostegana (Okada, 1978) currently contains thirty-nine described species. A number of Pseudostegana were collected from the fieldwork in southwestern China from 2010 to 2017. Eleven new species were discovered and are described from southwestern China: Pseudostegana alpina Zhang & Chen, sp. nov.; Pseudostegana amnicola Zhang & Chen, sp. nov.; Pseudostegana amoena Zhang & Chen, sp. nov.; Pseudostegana mailangang Zhang & Chen, sp. nov.; Pseudostegana meiduo Zhang & Chen, sp. nov.; Pseudostegana meiji Zhang & Chen, sp. nov.; Pseudostegana mystica Zhang & Chen, sp. nov.; Pseudostegana stictiptrata Zhang & Chen, sp. nov.; Pseudostegana stigmatptera Zhang & Chen, sp. nov.; Pseudostegana ximalaya Zhang & Chen, sp. nov. and Pseudostegana zhuoma Zhang & Chen, sp. nov. A key to all Chinese Pseudostegana species based on morphological characters is provided. Two mitochondrial loci (COI and ND2) and one nuclear locus (28S rRNA) were sequenced for the Pseudostegana specimens, and Bayesian and RAxML concatenated analyses were run. Molecular species delimitation is performed using the distance-based automatic barcode gap discovery (ABGD) method. Molecular data support the morphological characteristics observed among these Chinese species and confirm the new species as being distinctly different.

scholarly journals One new species of Cymbella C.A. Agardh (Bacillariophyta) from high altitude lakes in the Hengduan Mountains of Southwest China

2021 ◽  
Vol 80 (2) ◽  
pp. 184-190
Author(s):  
Qi Liu ◽  
Han Wu ◽  
Yan-Ling Li ◽  
John Patrick Kociolek
Keyword(s):  
Electron Microscopy ◽  
New Species ◽  
Southwest China ◽  
Central Area ◽  
Morphological Characteristics ◽  
Morphological Description ◽  
Hengduan Mountains ◽  
One New Species ◽  
A New Species ◽  
Scanning Electron

This paper describes a new species of the genus Cymbella C.A. Agardh from an alpine lake in the Hengduan Mountains of southwestern China. A detailed morphological description of the new species, named Cymbella luoyulanae sp. nov., is presented; the description is based on light and scanning electron microscopy. The main features of C. luoyulanae are strongly dorsiventral valves with strongly reverse-lateral raphe branches near the proximal ends, a large central area occupying approximately a half of the width with the valve and slit-like areolae comprising striae that may be unseriate or partially biseriate. The new species has morphological characteristics that resemble those of C. heihainensis Y.Li et Gong, C. modicepunctata Krammer and C. asiatica Metzeltin, Lange-Bertalot et Y.Li, but it differs from these three species in details of size, valve shape, striae density, central area, and number of stigmata.

Ceriporia albomellea (Phanerochaetaceae, Basidiomycota), a new species from tropical China based on morphological and molecular evidences

Phytotaxa ◽  
2017 ◽  
Vol 298 (1) ◽  
pp. 20 ◽  
Author(s):  
YUAN YUAN ◽  
XIAO-HONG JI ◽  
FANG WU ◽  
JIA-JIA CHEN
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Ribosomal Rna ◽  
Internal Transcribed Spacer ◽  
Large Subunit ◽  
Morphological Characteristics ◽  
Molecular Evidence ◽  
Its Regions ◽  
Tropical China ◽  
A New Species

A new polypore, Ceriporia albomellea, collected from tropical China, is described and illustrated based on morphological characteristics and molecular evidence. It is characterized by thin, resupinate basidiome with a white subiculum, cottony margin, white to cinnamon-buff pores, clavate cystidia and oblong-ellipsoid basidiospores measured as 3.1–3.8 × 1.7–2 µm. Phylogenetic analysis based on the internal transcribed spacer (ITS) regions and nuclear large subunit (nLSU) ribosomal RNA gene regions supported C. albomellea as a distinctive species belonging to Ceriporia.

A new species of Usnea (Parmeliaceae, lichenized Ascomycota) from Southwest China

Phytotaxa ◽  
2020 ◽  
Vol 472 (1) ◽  
pp. 23-32
Author(s):  
LIU-FU HAN ◽  
YU-HUAN XIE ◽  
HUAN-BING ZHANG ◽  
LI-SI LI ◽  
SHOU-YU GUO
Keyword(s):  
New Species ◽  
Southwest China ◽  
Sequence Data ◽  
Morphological Characteristics ◽  
Its Sequence ◽  
Nrdna Its ◽  
A New Species

During the study of Usnea from China, we identified and illustrated a new species, Usnea sulphuridiscoidea S. Y. Guo & L. F. Han from the Ailaoshan Mountain of Southwest China, based on morphological characteristics and nrDNA ITS sequence data. This new species can be distinguished morphologically from other apotheciate members of Usnea by the special lateral apothecia with sulphur disc, the inflated branches with glossy surface and numerous papillae as well as many fibrils, meanwhile lacking pseudocyphellae and soralia. The nrDNA ITS sequence data supported the recognition of the new species.

Clitopilus fusiformis (Entolomataceae; Agaricales), a new species from southwest China

Phytotaxa ◽  
2017 ◽  
Vol 321 (2) ◽  
pp. 201 ◽  
Author(s):  
DI WANG ◽  
WANG-QIU DENG ◽  
XIAO-LAN HE ◽  
WEI-HONG PENG ◽  
BING-CHENG GAN
Keyword(s):  
New Species ◽  
High Altitude ◽  
Southwest China ◽  
Molecular Evidence ◽  
New Taxon ◽  
Phylogenetic Affinities ◽  
A New Species

Clitopilus fusiformis, a new species in section Clitopilus, is described from high altitude areas in southwest China. Its detailed description and comparison with other similar members are presented. This new taxon is further confirmed by molecular evidence, and its phylogenetic affinities are discussed based on ITS and RPB2 sequences.

scholarly journals Twenty-six new species of Hoploscopa (Lepidoptera, Crambidae) from South-East Asia revealed by morphology and DNA barcoding

ZooKeys ◽  
2020 ◽  
Vol 907 ◽  
pp. 1-99 ◽  
Author(s):  
Théo Léger ◽  
Christian Kehlmaier ◽  
Charles S. Vairappan ◽  
Matthias Nuss
Keyword(s):  
New Species ◽  
East Asia ◽  
Species Delimitation ◽  
South East Asia ◽  
Museum Specimens ◽  
Cryptic Diversity ◽  
Undescribed Species ◽  
Allopatric Divergence ◽  
Scientific Collections ◽  
Molecular Characters

Hoploscopa Meyrick (Lepidoptera: Crambidae) is a fern-feeding genus found in montane areas of South-East Asia and Melanesia, eastwards up to the Samoan Islands. It includes sixteen described species, with at least 70 further undescribed species known from scientific collections. An iterative approach including morphological and molecular characters was used in order to explore the diversity of Hoploscopa. The hitherto described species are revised, and descriptions authored by T. Léger and M. Nuss are provided for an additional 26 new species: H. agtuuganonensissp. nov., H. albipunctasp. nov., H. albomaculatasp. nov., H. anacanthasp. nov., H. boletasp. nov., H. cynodontasp. nov., H. danaoensissp. nov., H. gombongisp. nov., H. gracilissp. nov., H. ignitamaculaesp. nov., H. isarogensissp. nov., H. jubatasp. nov., H. kelamasp. nov., H. kinabaluensissp. nov., H. mallyisp. nov., H. marijoweissaesp. nov., H. matheaesp. nov., H. niveofasciasp. nov., H. pangrangoensissp. nov., H. parvimaculasp. nov., H. pseudometacrossasp. nov., H. sepanggisp. nov., H. sumatrensissp. nov., H. titikasp. nov., H. tonsepisp. nov., H. ypsilonsp. nov. Using a protocol specific for the amplification of DNA from old museum specimens, we recovered 101 COI barcodes for all but one of the newly described species, with 76 being barcode compliant (>487 bp). Species delimitation analyses suggest cryptic diversity, with six cases reflecting allopatric divergence, and two further cases found in sympatry.

scholarly journals Taxonomic Revision of the Genus Lactifluus (Russulales, Basidiomycota) of South Korea

2021 ◽  
Author(s):  
Hyun Lee ◽  
Komsit Wissitrassameewong ◽  
Myung Soo Park ◽  
Jonathan J. Fong ◽  
Annemieke Verbeken ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
New Species ◽  
Morphological Variation ◽  
North American ◽  
Species Delimitation ◽  
Taxonomic Revision ◽  
Molecular Data ◽  
Similar Species ◽  
Cryptic Diversity ◽  
Intraspecific Morphological Variation

Abstract Lactifluus (Pers.) Roussel is an ectomycorrhizal genus that was recently recognized to be distinct from the genus Lactarius. To date, 216 Lactifluus species have been reported worldwide. Misidentification of Lactifluus species is common because of intraspecific morphological variation, cryptic diversity, and the limited number of taxonomic keys available. Molecular data are indispensable for species delimitation; a multilocus phylogenetic analysis showed that most Asian Lactifluus species are not conspecific with morphologically similar species present on other continents. In particular, Korea has misused European and North American Lactifluus names. In this study, we evaluated the taxonomy of Lactifluus in Korea using both morphological and multilocus molecular (ITS, nrLSU, rpb1, and rpb2) data. We examined 199 Lactifluus specimens collected between 1980 and 2016, and a total of 24 species across the four Lactifluus subgenera were identified. All Korean species are distinct and clearly separated from European and North American species. Five taxa corresponded to previously described species from Asia and the remaining 19 taxa are confirmed as new species. Herein, we provide keys to the Korean Lactifluus species within their subgenera, molecular phylogenies, a summary of diversity, and detailed description of the new species.

scholarly journals Four new species in the Tremella fibulifera complex (Tremellales, Basidiomycota)

MycoKeys ◽  
2021 ◽  
Vol 82 ◽  
pp. 33-56
Author(s):  
Long-Fei Fan ◽  
Renato Lúcio Mendes Alvarenga ◽  
Tatiana Baptista Gibertoni ◽  
Fang Wu ◽  
Yu-Cheng Dai
Keyword(s):  
New Species ◽  
Rna Polymerase Ii ◽  
Phylogenetic Analyses ◽  
Large Subunit ◽  
Elongation Factor ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Small Subunit ◽  
Molecular Evidence ◽  
Rrna Gene

Samples of species close to Tremella fibulifera from China and Brazil are studied, and T. fibulifera is confirmed as a species complex including nine species. Five known species (T. cheejenii, T. fibulifera s.s., T. “neofibulifera”, T. lloydiae-candidae and T. olens) and four new species (T. australe, T. guangxiensis, T. latispora and T. subfibulifera) in the complex are recognized based on morphological characteristics, molecular evidence, and geographic distribution. Sequences of eight species of the complex were included in the phylogenetic analyses because T. olens lacks molecular data. The phylogenetic analyses were performed by a combined sequence dataset of the internal transcribed spacer (ITS) and the partial nuclear large subunit rDNA (nLSU), and a combined sequence dataset of the ITS, partial nLSU, the small subunit mitochondrial rRNA gene (mtSSU), the translation elongation factor 1-α (TEF1), the largest and second largest subunits of RNA polymerase II (RPB1 and RPB2). The eight species formed eight independent lineages with robust support in phylogenies based on both datasets. Illustrated description of the six species including Tremella fibulifera s.s., T. “neofibulifera” and four new species, and discussions with their related species, are provided. A table of the comparison of the important characteristics of nine species in the T. fibulifera complex and a key to the whitish species in Tremella s.s. are provided.

scholarly journals Ottelia fengshanensis, a new bisexual species of Ottelia (Hydrocharitaceae) from southwestern China

PhytoKeys ◽  
2019 ◽  
Vol 135 ◽  
pp. 1-10
Author(s):  
Zhi-Zhong Li ◽  
Shuang Wu ◽  
Chun-Yu Zhou ◽  
Yan Liu ◽  
Guang-Wan Hu ◽  
...  
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Species Delimitation ◽  
Southwest China ◽  
Morphological Features ◽  
Bisexual Species ◽  
Molecular Phylogenetic ◽  
Dark Green ◽  
Close Relatives ◽  
A New Species

Ottelia fengshanensis, a new species (Hydrocharitaceae) from southwest China is here described and illustrated. Comparing its morphological features to putative close relatives O. guanyangensis, it has 3–4 flowers (vs. 2–5) each spathe, hexagonal-cylindric fruit, white styles (vs. yellow), green leaves (vs. dark green) and fruit tiny winged (vs. winged obviously). Molecular phylogenetic investigation of four DNA sequences (ITS, rbcL, trnK5’ intron and trnS-trnG) and the Poisson Tree Processes model for species delimitation (PTP) analysis, further resolves O. fengshanensis as a new species that is close to O. guanyangensis with distinct support.

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