scholarly journals Genetic analysis reveals Finnish Formica fennica populations do not form a separate genetic entity from F. exsecta

PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e6013 ◽  
Author(s):  
Sanja Maria Hakala ◽  
Perttu Seppä ◽  
Maria Heikkilä ◽  
Pekka Punttila ◽  
Jouni Sorvari ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Morphological Characters ◽  
Coi Gene ◽  
Species Status ◽  
Separate Species ◽  
Mitochondrial Coi ◽  
Recent Addition ◽  
Morphological Differences ◽  
Dna Microsatellite ◽  
Dna Microsatellite Markers

Coptoformica Müller, 1923 is a subgenus of Formica Linnaeus, 1758 that consists of c. a dozen species of ants that typically inhabit open grassy habitats and build small nest mounds. The most recent addition to the group is Formica fennica Seifert, 2000. The description was based on morphological characters, but the species status has not been confirmed by molecular methods. In this study, we use thirteen DNA microsatellite markers and a partial mitochondrial COI gene sequence to assess the species status of F. fennica, by comparing the genetic variation among samples identified as F. fennica and six other boreal Formica (Coptoformica) species. Most of the species studied form separate, discontinuous clusters in phylogenetic and spatial analyses with only little intraspecific genetic variation. However, both nuclear and mitochondrial markers fail to separate the species pair F. exsecta Nylander, 1846 and F. fennica despite established morphological differences. The genetic variation within the F. exsecta/fennica group is extensive, but reflects spatial rather than morphological differences. Finnish F. fennica populations studied so far should not be considered a separate species, but merely a morph of F. exsecta.

Mitochondrial DNA sequence evidence supporting the recognition of a new North Atlantic Pseudostichopus species (Echinodermata: Holothuroidea)

2004 ◽  
Vol 84 (5) ◽  
pp. 1077-1084 ◽  
Author(s):  
Francisco A. Solís-Marín ◽  
David S.M. Billett ◽  
Joanne Preston ◽  
Alex D. Rogers
Keyword(s):  
Dna Sequences ◽  
Mitochondrial Gene ◽  
Distinct Species ◽  
Molecular Study ◽  
Morphological Characters ◽  
Coi Gene ◽  
Species Status ◽  
Separate Species ◽  
The Family ◽  
A New Species

A new species of the synallactid sea cucumber genus Pseudostichopus is described, P. aemulatus sp. nov., based on genetic (DNA sequences of the mitochondrial gene Cytochrome Oxidase I [COI] gene) and morphological characters. A comparative molecular study with two other species of the same genus (P. villosus and P. mollis) and from a different family (Isostichopus fuscus) was carried out in order to clarify its taxonomic identity. The nucleotide distance between P. aemulatus sp. nov. and P. villosus and P. mollis is sufficient to support distinct species status. The estimated difference in the number of amino acids, coded for by a partially sequenced COI gene, within the species of the family Synallactidae ranged from 4 to 18. The phylogenetic analysis clearly supports separate species status of these sympatric morphotypes, as indicated by the morphological analysis.

Are Tetranychus macfarlanei Baker & Pritchard and Tetranychus malaysiensis Ehara (Acari: Tetranychidae) one species? Morphological and molecular evidences for synonymy between these two spider mite species and a note on invasiveness of T. macfarlanei on okra and eggplant in India

2017 ◽  
Vol 22 (4) ◽  
pp. 467
Author(s):  
Mahran Zeity ◽  
Nagappa Srinivas ◽  
Chinnamade Channegowde Gowda
Keyword(s):  
Spider Mite ◽  
Morphological Characters ◽  
Molecular Data ◽  
Mite Species ◽  
Coi Gene ◽  
High Similarity ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
High Degree ◽  
Degree Of Similarity

Study of morphological characters of Tetranychus macfarlanei Baker & Pritchard and Tetranychus malaysiensis Ehara revealed high similarity by comparing all the important characters in addition to the characters pointed out by Ehara to separate those two species. Molecular phylogeny of seven Indian populations of T. macfarlanei and one population of T. malaysiensis from Philippines along with few distantly related species of Tetranychus was attempted. High degree of similarity between these two species at mitochondrial COI gene (96%) as well as ITS2 (rDNA) (96–99%) region was evident. Based on both morphological features and molecular data, T. malaysiensis is proposed as a junior synonym of T. macfarlanei based on ICZN’s law of priority. Also more female characters are prompted in this study to distinctly discriminate T. macfarlanei from its most resembling species, Tetranychus ludeni Zacher. Tetranychus macfarlanei has emerged as a pest of several cultivated crop plants in India. 

A new species of Cyrtodactylus (Squamata: Gekkonidae) from Ninh Thuan Province, southern Vietnam

Zootaxa ◽  
2021 ◽  
Vol 4999 (1) ◽  
pp. 58-76
Author(s):  
Quyen Hanh Do ◽  
TRUNG MY PHUNG ◽  
HANH THI NGO ◽  
MINH DUC LE ◽  
THOMAS ZIEGLER ◽  
...  
Keyword(s):  
New Species ◽  
Phylogenetic Analyses ◽  
Coi Gene ◽  
Unique Combination ◽  
Mitochondrial Coi ◽  
Southern Vietnam ◽  
Mitochondrial Coi Gene ◽  
Morphological Differences ◽  
Dorsal Pattern ◽  
A New Species

A new species of the Cyrtodactylus irregularis group is described from Ninh Thuan Province, southern Vietnam based on molecular divergence and morphological differences. Cyrtodactylus orlovi sp. nov. is distinguished from the remaining Indochinese bent-toed geckos by having the unique combination of the following characters: size medium (SVL 61.0–77.7 mm); dorsal tubercles in 16–20 irregular rows; 36–39 ventral scale rows; precloacal pores absent in females, 5 or 6 in males, in a continuous row; femoral pores absent; 3–8 enlarged femoral scales; postcloacal spurs 1 or 2; lamellae under toe IV 16–19; a continuous neckband; a highly irregular transverse banded dorsal pattern; the absence of transversely enlarged median subcaudal scales. In phylogenetic analyses, the new species was revealed to be the sister taxon to a clade consisting of Cyrtodactylus cattienensis and the most recently described species from Vietnam, C. chungi, with 12.1–12.4% and 11.7 % pairwise genetic divergence from the two species, respectively, based on a fragment of the mitochondrial COI gene.  

A revision of the dark-bellied, stream-dwelling snakes of the genus Hebius (Reptilia: Squamata: Natricidae) with the description of a new species from China, Vietnam and Thailand

Zootaxa ◽  
2021 ◽  
Vol 4911 (1) ◽  
pp. 1-61
Author(s):  
PATRICK DAVID ◽  
GERNOT VOGEL ◽  
TRUONG QUANG NGUYEN ◽  
NIKOLAI L. ORLOV ◽  
OLIVIER S. G. PAUWELS ◽  
...  
Keyword(s):  
New Species ◽  
Southern China ◽  
Morphological Characters ◽  
Species Status ◽  
Distribution Maps ◽  
Northern Vietnam ◽  
Full Species ◽  
North Eastern ◽  
Morphological Differences ◽  
A New Species

Species of the genus Hebius Thompson, 1913 with 17 or 19 dorsal scale rows at midbody and an overall dark venter are reviewed, including the two species previously known as Parahelicops annamensis Bourret, 1934 and Pararhabdophis chapaensis Bourret, 1934. Specimens with 17 scale rows are morphologically similar to Hebius venningi (Wall, 1910), which is here redefined based on external morphological characters such as scalation, and dorsal and ventral patterns. Consequently, Natrix nigriventer Wall, 1925 is resurrected from its synonymy with Hebius venningi, whereas Natrix taronensis Smith, 1940, previously considered a subspecies of H. venningi or a full species by some authors but without justification, is here confirmed to full species status. Another group of species, mostly similar in coloration and pattern to the H. venningi group but with 19 dorsal scale rows, includes H. modestus (Günther, 1875), H. deschauenseei (Taylor, 1934) and a new species which is described herein based on specimens from northern Vietnam, southern China and north-eastern Thailand due to distinct morphological differences. We also provide updated taxonomic accounts for the species of this group, including an identification key and distribution maps. 

scholarly journals Discovery of Eubasilissa signata Wiggins, 1998 (Trichoptera, Phryganeidae) from Taiwan

Zoosymposia ◽  
2019 ◽  
Vol 14 (1) ◽  
pp. 103-107
Author(s):  
RYOICHI B. KURANISHI ◽  
LI-PENG HSU
Keyword(s):  
Dna Barcode ◽  
Species Group ◽  
Coi Gene ◽  
Single Female ◽  
Separate Species ◽  
Other Information ◽  
Group A ◽  
Morphological Differences ◽  
Male Morphology ◽  
First Time

The phryganeid caddisfly Eubasilissa signata Wiggins, 1998 was originally described based on a single female specimen from Korea, and no other information on the species was available. Here we record an additional five male and five female specimens of E. signata from Taiwan and describe the male morphology for the first time. Based on morphological differences with other Eubasilissa species, E. signata should be regarded as a separate species and also not a member of the “tibetana” species group. A 658-bp fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene of the new specimens of E. signata is provided as a DNA barcode.

Magnolia sinostellata and relatives (Magnoliaceae)

Phytotaxa ◽  
2013 ◽  
Vol 154 (1) ◽  
pp. 47 ◽  
Author(s):  
YA-LING WANG ◽  
ERLAND EJDER ◽  
JIAN-FEN YANG ◽  
RAO LIU ◽  
LI-MING YE ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Plastid Dna ◽  
Distinct Species ◽  
Morphological Characters ◽  
Phylogenetic Study ◽  
Wild Populations ◽  
Species Status ◽  
Separate Species ◽  
Magnolia Sinostellata ◽  
Normal Meiosis

Magnolia sinostellata has been considered a synonym of Magnolia stellata by several taxonomists due to many shared morphological characters. With similar leaves and twigs, Magnolia amoena is distributed in areas near M. sinostellata. These three species were studied by comparing morphological, cytological and palynological characters, creating a maximum parsimony phylogenetic tree based on plastid DNA sequences and studying these taxa in the field. The results are as follows: M. sinostellata is a diploid, 2n=2x=38, and there are heterozygotes with paracentric inversion chromosomes in wild populations. Magnolia stellata is also a diploid, and there are heterozygotes with pericentric inversion chromosomes in wild populations. The abnormal chromosome behaviour in meiosis has serious effects on survival of the two species. Magnolia amoena is diploid with more or less normal meiosis except for a few lagging chromosomes in anaphase I and II. Magnolia stellata has a more complicated exine sculpture than the other two; exine structure is different in all three species. Separate species status for M. sinostellata is also supported by results of the plastid DNA phylogenetic study. Distributions, population descriptions and observations are provided, and based on all the evidence presented we conclude that M. sinostellata is a distinct species in M. subgenus Yulania. 

scholarly journals Molecular Identity of Subterranean Termites

2020 ◽  
Vol 8 (4) ◽  
pp. 410-416
Author(s):  
Srinivasa Murthy Kotilingam
Keyword(s):  
Sequence Analysis ◽  
Life Stage ◽  
Morphological Characters ◽  
Caste System ◽  
Coi Gene ◽  
Subterranean Termites ◽  
Morphological Identification ◽  
Sequence Information ◽  
Molecular Sequence ◽  
Morphological Differences

Identification of termites is a challenging task due to variable morphological differences among species and their eusocial behaviour with different caste system. A method for easy and accurate species-level identification at any life stage is required. In this study, a 658-base pair region of the mitochondrial cytochrome oxidase I (COI) gene was used to explore its utility in the identification of important subterranean termites. Termite specimens collected from 25 locations from various states, were characterized using specific primers for their identification. Sequence analysis and divergence among the species was assessed. The composition of the mitochondrial sequence of the COI gene in the present study was expectedly AT biased. GenBank accession numbers were obtained for the species. Sequence analysis and divergence among the species was assessed, which revealed significantly high percentage of A+T base composition. Molecular sequence information from NCBI revealed relatedness in all the collected termites, accurately as revealed by their morphological characters. The studies indicate the relevance of DNA sequencing to match different forms of termites and address ambiguities in morphological identification. The information on species diversity would help plan strategies for management of termites. Int. J. Appl. Sci. Biotechnol. Vol 8(4): 410-416

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