scholarly journals Promotion of growth and metal accumulation of alfalfa by coinoculation with Sinorhizobium and Agrobacterium under copper and zinc stress

PeerJ ◽  
2019 ◽  
Vol 7 ◽  
pp. e6875 ◽  
Author(s):  
Liru Jian ◽  
Xiaoli Bai ◽  
Hui Zhang ◽  
Xiuyong Song ◽  
Zhefei Li
Keyword(s):  
Agrobacterium Tumefaciens ◽  
Plant Growth ◽  
Contaminated Soils ◽  
Sinorhizobium Meliloti ◽  
Plant Biomass ◽  
Antioxidant Activities ◽  
Synergistic Effects ◽  
Medicago Lupulina ◽  
Nitrogenase Activities ◽  
Zn Stress

The Legume-Rhizobium symbiosis has been proposed as a promising technique for the phytoremediation of contaminated soils due to its beneficial activity in symbiotic nitrogen fixation. However, numerous studies have shown that excessive heavy metals reduce the efficiency of symbiotic nodulation with Rhizobium and inhibit plant growth. In this study, we aimed to evaluate the synergistic effects of IAA-producing bacteria and Rhizobium on Medicago lupulina growth under Cu and Zn stress. Pot experiments showed that 400 mg kg−1 Cu2 + and Zn2 + greatly inhibited plant growth, but dual inoculation of Medicago lupulina with Sinorhizobium meliloti CCNWSX0020 and Agrobacterium tumefaciens CCNWGS0286 significantly increased the number of nodules and plant biomass by enhancing antioxidant activities. Under double stress of 400 mg kg−1 Cu2 + and Zn2 +, the nodule number and nitrogenase activities of dual-inoculated plants were 48.5% and 154.4% higher, respectively, than those of plants inoculated with Sinorhizobium meliloti. The root and above-ground portion lengths of the dual-inoculated plants were 32.6% and 14.1% greater, respectively, than those of the control, while the root and above-ground portion dry weights were 34.3% and 32.2% greater, respectively, than those of the control. Compared with S. meliloti and A. tumefaciens single inoculation, coinoculation increased total Cu uptake by 39.1% and 47.5% and increased total Zn uptake by 35.4% and 44.2%, respectively, under double metal stress conditions. Therefore, coinoculation with Sinorhizobium meliloti and Agrobacterium tumefaciens enhances metal phytoextraction by increasing plant growth and antioxidant activities under Cu/Zn stress, which provides a new approach for bioremediation in heavy metal-contaminated soil.

scholarly journals Divergence of compost extract and bio-organic manure effects on lucerne plant and soil

PeerJ ◽  
2017 ◽  
Vol 5 ◽  
pp. e3775 ◽  
Author(s):  
Haiyan Ren ◽  
Jian Hu ◽  
Yifei Hu ◽  
Gaowen Yang ◽  
Yingjun Zhang
Keyword(s):  
Plant Growth ◽  
Nutritive Value ◽  
Plant Biomass ◽  
Synergistic Effects ◽  
Soil Health ◽  
Treatment Plant ◽  
Organic Manure ◽  
Economic Returns ◽  
Rhizobium Inoculation ◽  
Compost Extract

AimApplication of organic materials into agricultural systems enhances plant growth and yields, and improves soil fertility and structure. This study aimed to examine the effects of “compost extract (CE)”, a soil conditioner, and bio-organic manure (BOM) on the growth of lucerne (Medicago sativa), and compare the efficiency between BOM (including numbers of microorganisms) and CE (including no added microorganisms).MethodA greenhouse experiment was conducted with four soil amendment treatments (control, BOM, CE and CEBOM), and was arranged in a completely randomized design with 10 replicates for each treatment. Plant biomass, nutritive value and rhizobia efficacy as well as soil characteristics were monitored.ResultCE rather than BOM application showed a positive effect on plant growth and soil properties when compared with the control. Lucerne nodulation responded equally to CE addition and rhizobium inoculation. CE alone and in combination with BOM significantly increased plant growth and soil microbial activities and improved soil structure. The synergistic effects of CE and BOM indicate that applying CE and BOM together could increase their efficiency, leading to higher economic returns and improved soil health. However, CE alone is more effective for legume growth since nodulation was suppressed by nitrogen input from BOM. CE had a higher efficiency than BOM for enriching soil indigenous microorganisms instead of adding microorganisms and favouring plant nodulation.

scholarly journals Transcriptome Response to Heavy Metals in Sinorhizobium meliloti CCNWSX0020 Reveals New Metal Resistance Determinants That Also Promote Bioremediation by Medicago lupulina in Metal-Contaminated Soil

2017 ◽  
Vol 83 (20) ◽  
Author(s):  
Mingmei Lu ◽  
Shuo Jiao ◽  
Enting Gao ◽  
Xiuyong Song ◽  
Zhefei Li ◽  
...  
Keyword(s):  
Heavy Metal ◽  
Contaminated Soils ◽  
Sinorhizobium Meliloti ◽  
Heavy Metal Resistance ◽  
Metal Resistance ◽  
Content Type ◽  
Resistance Determinants ◽  
Medicago Lupulina ◽  
Legume Symbiosis ◽  
Cu And Zn

ABSTRACT The symbiosis of the highly metal-resistant Sinorhizobium meliloti CCNWSX0020 and Medicago lupulina has been considered an efficient tool for bioremediation of heavy metal-polluted soils. However, the metal resistance mechanisms of S. meliloti CCNWSX00200 have not been elucidated in detail. Here we employed a comparative transcriptome approach to analyze the defense mechanisms of S. meliloti CCNWSX00200 against Cu or Zn exposure. Six highly upregulated transcripts involved in Cu and Zn resistance were identified through deletion mutagenesis, including genes encoding a multicopper oxidase (CueO), an outer membrane protein (Omp), sulfite oxidoreductases (YedYZ), and three hypothetical proteins (a CusA-like protein, a FixH-like protein, and an unknown protein), and the corresponding mutant strains showed various degrees of sensitivity to multiple metals. The Cu-sensitive mutant (ΔcueO) and three mutants that were both Cu and Zn sensitive (ΔyedYZ, ΔcusA-like, and ΔfixH-like) were selected for further study of the effects of these metal resistance determinants on bioremediation. The results showed that inoculation with the ΔcueO mutant severely inhibited infection establishment and nodulation of M. lupulina under Cu stress, while inoculation with the ΔyedYZ and ΔfixH-like mutants decreased just the early infection frequency and nodulation under Cu and Zn stresses. In contrast, inoculation with the ΔcusA-like mutant almost led to loss of the symbiotic capacity of M. lupulina to even grow in uncontaminated soil. Moreover, the antioxidant enzyme activity and metal accumulation in roots of M. lupulina inoculated with all mutants were lower than those with the wild-type strain. These results suggest that heavy metal resistance determinants may promote bioremediation by directly or indirectly influencing formation of the rhizobium-legume symbiosis. IMPORTANCE Rhizobium-legume symbiosis has been promoted as an appropriate tool for bioremediation of heavy metal-contaminated soils. Considering the plant-growth-promoting traits and survival advantage of metal-resistant rhizobia in contaminated environments, more heavy metal-resistant rhizobia and genetically manipulated strains were investigated. In view of the genetic diversity of metal resistance determinants in rhizobia, their effects on phytoremediation by the rhizobium-legume symbiosis must be different and depend on their specific assigned functions. Our work provides a better understanding of the mechanism of heavy metal resistance determinants involved in the rhizobium-legume symbiosis, and in further studies, genetically modified rhizobia harboring effective heavy metal resistance determinants may be engineered for the practical application of rhizobium-legume symbiosis for bioremediation in metal-contaminated soils.

Bioremediation model of oil-contaminated soil in Lapindo mud using multisymbiotic organism

2020 ◽  
Vol 31 (3) ◽  
pp. 586-601
Author(s):  
Yuni Sri Rahayu
Keyword(s):  
Plant Growth ◽  
Contaminated Soil ◽  
Contaminated Soils ◽  
Plant Biomass ◽  
Root Nodules ◽  
Interaction Pattern ◽  
Total Petroleum Hydrocarbons ◽  
Phosphate Solubilizing Bacteria ◽  
Content Type ◽  
Tripartite Symbiosis

PurposeThe study aimed at developing the bioremediation model of Lapindo mud through multisymbiotic organism.Design/methodology/approachThe research was conducted using completely randomized design. The model plants chosen in this research were soybean. The interaction pattern during the treatment was used to develop the bioremediation model based on the parameters.FindingsThe results showed that there was an effect of the type of organism on the parameters, namely: the growth of plant (biomass, height, length of root, and number of leaves), the biomass of root nodules, the percentage of mycorrhizal infection, the content of water, nitrogen, phosphorus, and total petroleum hydrocarbons (TPHs). There was a pattern of multisymbiotic interaction between each organism and roles of each symbiont in that interaction. Therefore, the plants were capable of surviving in the environment of Sidoarjo Lapindo mud. This pattern can be named as the bioremediation model proposed, which is the analogy of tripartite symbiosis between plants, mycorrhizae, and Rhizobium but also adding plant growth bacteria such as phosphate-solubilizing bacteria and hydrocarbon degradation bacteria. The implementation of this model can be used to treat oil-contaminated soil in order to be used as a plant growth medium.Originality/valuePhytoremediation is a new and promising approach to remove contaminants in the environment but using plants alone for remediation confronts many limitations. Therefore, the application of plant-growth-promoting rhizobia (PGPR) has been extended to remediate contaminated soils in association with plants (Zhuang et al., 2007). The development of the model will use the analogy of tripartite symbiosis between plants, mycorrhizae, and Rhizobium. The developed model will be based on the interaction pattern on each parameters obtained. Bioremediation is chosen because it is considered an effective technique to transform toxic components into less toxic products without disrupting the surrounding environment. Besides, bioremediation is cheaper and environment-friendly because it utilizes microorganisms to clean pollutants from the environment (Nugroho, 2006).

Effects of 1-aminocyclopropane-1-carboxylate (ACC) deaminase-overproducing Sinorhizobium meliloti on plant growth and copper tolerance of Medicago lupulina

2015 ◽  
Vol 391 (1-2) ◽  
pp. 383-398 ◽  
Author(s):  
Zhaoyu Kong ◽  
Bernard R. Glick ◽  
Jin Duan ◽  
Shulan Ding ◽  
Jie Tian ◽  
...  
Keyword(s):  
Plant Growth ◽  
Sinorhizobium Meliloti ◽  
Acc Deaminase ◽  
Copper Tolerance ◽  
Medicago Lupulina

scholarly journals Species-specific synergistic effects of two plant growth—promoting microbes on green roof plant biomass and photosynthetic efficiency

PLoS ONE ◽  
2018 ◽  
Vol 13 (12) ◽  
pp. e0209432 ◽  
Author(s):  
Long Xie ◽  
Susanna Lehvävirta ◽  
Sari Timonen ◽  
Jutta Kasurinen ◽  
Juhamatti Niemikapee ◽  
...  
Keyword(s):  
Plant Growth ◽  
Photosynthetic Efficiency ◽  
Plant Biomass ◽  
Synergistic Effects ◽  
Green Roof ◽  
Plant Growth Promoting ◽  
Growth Promoting ◽  
Plant Growth Promoting Microbes ◽  
Species Specific

The synergistic effects of sucrose and plant growth regulators on morphogenesis and evaluation of antioxidant activities in regenerated tissues of Caralluma tuberculata

2016 ◽  
Vol 38 (8) ◽  
Author(s):  
Roshan Zamir ◽  
Shahid Akbar Khalil ◽  
Nisar Ahmad ◽  
Abdur Rab ◽  
Syed Tariq Shah ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Antioxidant Activities ◽  
Synergistic Effects

scholarly journals Silicon substances for restoration of oil-contaminated areas

2021 ◽  
Vol 931 (1) ◽  
pp. 012015
Author(s):  
P Zhang ◽  
V V Matichenkov ◽  
E A Bocharnikova ◽  
S M Sevostianov
Keyword(s):  
Plant Growth ◽  
Plant Biomass ◽  
Antioxidant Activities ◽  
Water Soluble ◽  
Soil Reclamation ◽  
Plant Tolerance ◽  
Soil Enzymatic Activity ◽  
Soil Microbial ◽  
Grey Forest Soil ◽  
Soil Microbial Population

Abstract Numerous investigations demonstrate that active forms of silicon (Si) enhance the plant tolerance against abiotic stresses by several mechanisms, including increasing the antioxidant activities and minimizing oxidative damage. Soil contamination with oil and oil products relates to abiotic stress that detrimentally affects soil microbial population and plant growth. Considering the crucial role of microorganisms and plants in bioremediation of oil-polluted areas, Si substances can be beneficial to acceleration of soil reclamation. In greenhouse experiment, wheat was grown in Grey Forest Soil contaminated with used motor oil. The effect of fumed silica and monosilicic acid on soil enzymatic activity and plant growth was studied. Both Si substances provided increasing the plant biomass and the activities of catalase and dehydrogenase. As regards the plant growth, the effect of Si was more pronounced in polluted soil, while the enzyme activity was higher affected in unpolluted soil. The activities of catalase and dehydrogenase were closely correlated to the water-soluble Si in soil (R=0.91-0.92). Silicon substances with high content of, plant-and microorganism-available Si might be promising for involvement in bioremediation technology for oil-contaminated soil.

scholarly journals Improved chromium tolerance of Medicago sativa by plant growth-promoting rhizobacteria (PGPR)

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Nabil Tirry ◽  
Aziza Kouchou ◽  
Bouchra El Omari ◽  
Mohamed Ferioun ◽  
Naïma El Ghachtouli
Keyword(s):  
Plant Growth ◽  
Medicago Sativa ◽  
Contaminated Soils ◽  
Plant Biomass ◽  
Growth Promotion ◽  
Plant Growth Promoting Rhizobacteria ◽  
Biochemical Tests ◽  
Pgp Traits ◽  
Plant Growth Promoting ◽  
Growth Promoting

Abstract Background Soil pollution by heavy metals increases the bioavailability of metals like hexavalent chromium (Cr (VI)), subsequently limiting plant growth and reducing the efficiency of phytoremediation. Plant growth-promoting rhizobacteria (PGPR) have substantial potential to enhance plant growth as well as plant tolerance to metal stress. The aim of this research was to investigate Cr (VI) phytoremediation enhancement by PGPR. Results The results showed that the 27 rhizobacterial isolates studied were confirmed as Cr (VI)-resistant PGPR, by using classical biochemical tests (phosphate solubilization, nitrogen fixation, indole acetic acid, exopolysaccharides, hydrogen cyanide, siderophores, ammonia, cellulase, pectinase, and chitinase production) and showed variable levels of Cr (VI) resistance (300–600 mg/L). The best four selected Cr (VI)-resistant PGPR (NT15, NT19, NT20, and NT27) retained most of the PGP traits in the presence of 100–200 mg/L concentrations of Cr (VI). The inoculation of Medicago sativa with any of these four isolates improved the shoot and root dry weight. The NT27 isolate identified using 16S rDNA gene sequence analyses as a strain of Pseudomonas sp. was most effective in terms of plant growth promotion and stress level decrease. It increased shoot and root dry weights of M. sativa by 97.6 and 95.4%, respectively, in the presence of Cr (VI) when compared to non-inoculated control plants. It also greatly increased chlorophyll content and decreased the levels of stress markers, malondialdehyde, hydrogen peroxide, and proline. The results of the effect of Pseudomonas sp. on Cr content and bioaccumulation factor (BAF) of the shoots and roots of M. sativa plants showed the increase of plant biomass concomitantly with the increase of Cr root concentration in inoculated plants. This would lead to a higher potential of Cr (VI) phytostabilization. Conclusions This study demonstrates that the association M. sativa-Pseudomonas sp. may be an efficient biological system for the bioremediation of Cr (VI)-contaminated soils.

scholarly journals Metal Accumulation Profile of Catharanthus roseus (L.) G.Don and Celosia argentea L. with EDTA Co-Application

Processes ◽  
2021 ◽  
Vol 9 (4) ◽  
pp. 598
Author(s):  
Muneeba Qurban ◽  
Cyrus Raza Mirza ◽  
Aqib Hassan Ali Khan ◽  
Walid Khalifa ◽  
Mustapha Boukendakdji ◽  
...  
Keyword(s):  
Plant Growth ◽  
Catharanthus Roseus ◽  
Metal Uptake ◽  
Metal Accumulation ◽  
Metal Tolerance ◽  
Plant Biomass ◽  
Ethylenediaminetetraacetic Acid ◽  
Ornamental Plants ◽  
Environmental Deterioration ◽  
Celosia Argentea

The problem of metal-induced toxicity is proliferating with an increase in industrialization and urbanization. The buildup of metals results in severe environmental deterioration and harmful impacts on plant growth. In this study, we investigated the potential of two ornamental plants, Catharanthus roseus (L.) G.Don and Celosia argentea L., to tolerate and accumulate Ni, Cr, Cd, Pb, and Cu. These ornamental plants were grown in Hoagland’s nutrient solution containing metal loads (50 µM and 100 µM) alone and in combination with a synthetic chelator, ethylenediaminetetraacetic acid (EDTA) (2.5 mM). Plant growth and metal tolerance varied in both plant species for Ni, Cr, Cd, Pb, and Cu. C. roseus growth was better in treatments without EDTA, particularly in Ni, Cr, and Pb treatments, and Pb content increased in all parts of the plant. In contrast, Cd content decreased with EDTA addition. In C. argentea, the addition of EDTA resulted in improved plant biomass at both doses of Cu. In contrast, plant biomass reduced significantly in the case of Ni. In C. argentea, without EDTA, root length in Cd and Cu treatments was significantly lower than the control and other treatments. However, the addition of EDTA resulted in improved growth at both doses for Pb and Cu. Metal accumulation in C. argentea enhanced significantly with EDTA addition at both doses of Cu and Cd. Hence, it can be concluded that EDTA addition resulted in improved growth and better metal uptake than treatments without EDTA. Metal accumulation increased with EDTA addition compared to treatments without EDTA, particularly for Pb in C. roseus and Cu and Cd in C. argentea. Based on the present results, C. roseus showed a better ability to phytostabilize Cu, Cd, and Ni, while C. argentea worked better for Ni, Cd, Cu, and Pb.

Sign in / Sign up

Export Citation Format

Share Document